Sublethal ciprofloxacin treatment leads to resistance via antioxidant systems in Proteus mirabilis
- Autores
- Aiassa, Virginia; Barnes, Ana Isabel; Smania, Andrea; Albesa, Inés
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- This study investigates new aspects of the possible role of antioxidant defenses in the mechanisms of resistance to ciprofloxacin in Proteus mirabilis. Four ciprofloxacin-resistant variants (CRVs), selected in vitro by repeated cultures in a subminimum inhibitory concentration (MIC) concentration of ciprofloxacin, attained different levels of antibiotic resistance and high Ferric reducing antioxidant power, with 10 6 frequencies. However, no mutations occurred in positions 83 or 87 of gyrA, 464 or 466 of gyrB, or 78, 80 or 84 of parC, suggesting that resistance took place without these typical mutations in DNA gyrase or topoisomerase IV. Assays with ciprofloxacin and the pump inhibitor carbonyl cyanide m-chlorophenylhydrazone showed that in addition to the antioxidant mechanisms, the influx/efflux mechanism also contributed to the increase in the resistance to ciprofloxacin in one CRV. Moreover, lipid oxidation to malondialdehyde and protein oxidation to carbonyls and advanced oxidation protein products were higher in sensitive than in the resistant strains, as a new factor involved in the mechanisms of resistance in P. mirabilis. The oxidative stress cross-resistance to telluride in CRVs enhanced the role of the antioxidants in the ciprofloxacin resistance of P. mirabilis, which was reinforced during the assays of reduction of susceptibility to ciprofloxacin by glutathione and ascorbic acid.
Fil: Aiassa, Virginia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Barnes, Ana Isabel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Smania, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Albesa, Inés. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina - Materia
-
Proteus mirabilis
ciprofloxacina
antioxidantes
resistencia a antibióticos - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/269086
Ver los metadatos del registro completo
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Sublethal ciprofloxacin treatment leads to resistance via antioxidant systems in Proteus mirabilisAiassa, VirginiaBarnes, Ana IsabelSmania, AndreaAlbesa, InésProteus mirabilisciprofloxacinaantioxidantesresistencia a antibióticoshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1This study investigates new aspects of the possible role of antioxidant defenses in the mechanisms of resistance to ciprofloxacin in Proteus mirabilis. Four ciprofloxacin-resistant variants (CRVs), selected in vitro by repeated cultures in a subminimum inhibitory concentration (MIC) concentration of ciprofloxacin, attained different levels of antibiotic resistance and high Ferric reducing antioxidant power, with 10 6 frequencies. However, no mutations occurred in positions 83 or 87 of gyrA, 464 or 466 of gyrB, or 78, 80 or 84 of parC, suggesting that resistance took place without these typical mutations in DNA gyrase or topoisomerase IV. Assays with ciprofloxacin and the pump inhibitor carbonyl cyanide m-chlorophenylhydrazone showed that in addition to the antioxidant mechanisms, the influx/efflux mechanism also contributed to the increase in the resistance to ciprofloxacin in one CRV. Moreover, lipid oxidation to malondialdehyde and protein oxidation to carbonyls and advanced oxidation protein products were higher in sensitive than in the resistant strains, as a new factor involved in the mechanisms of resistance in P. mirabilis. The oxidative stress cross-resistance to telluride in CRVs enhanced the role of the antioxidants in the ciprofloxacin resistance of P. mirabilis, which was reinforced during the assays of reduction of susceptibility to ciprofloxacin by glutathione and ascorbic acid.Fil: Aiassa, Virginia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Barnes, Ana Isabel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; ArgentinaFil: Smania, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Albesa, Inés. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; ArgentinaWiley Blackwell Publishing, Inc2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/269086Aiassa, Virginia; Barnes, Ana Isabel; Smania, Andrea; Albesa, Inés; Sublethal ciprofloxacin treatment leads to resistance via antioxidant systems in Proteus mirabilis; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 327; 1; 2-2012; 25-320378-1097CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article-abstract/327/1/25/534190?redirectedFrom=fulltextinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6968.2011.02453.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:16Zoai:ri.conicet.gov.ar:11336/269086instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:16.388CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Sublethal ciprofloxacin treatment leads to resistance via antioxidant systems in Proteus mirabilis |
title |
Sublethal ciprofloxacin treatment leads to resistance via antioxidant systems in Proteus mirabilis |
spellingShingle |
Sublethal ciprofloxacin treatment leads to resistance via antioxidant systems in Proteus mirabilis Aiassa, Virginia Proteus mirabilis ciprofloxacina antioxidantes resistencia a antibióticos |
title_short |
Sublethal ciprofloxacin treatment leads to resistance via antioxidant systems in Proteus mirabilis |
title_full |
Sublethal ciprofloxacin treatment leads to resistance via antioxidant systems in Proteus mirabilis |
title_fullStr |
Sublethal ciprofloxacin treatment leads to resistance via antioxidant systems in Proteus mirabilis |
title_full_unstemmed |
Sublethal ciprofloxacin treatment leads to resistance via antioxidant systems in Proteus mirabilis |
title_sort |
Sublethal ciprofloxacin treatment leads to resistance via antioxidant systems in Proteus mirabilis |
dc.creator.none.fl_str_mv |
Aiassa, Virginia Barnes, Ana Isabel Smania, Andrea Albesa, Inés |
author |
Aiassa, Virginia |
author_facet |
Aiassa, Virginia Barnes, Ana Isabel Smania, Andrea Albesa, Inés |
author_role |
author |
author2 |
Barnes, Ana Isabel Smania, Andrea Albesa, Inés |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Proteus mirabilis ciprofloxacina antioxidantes resistencia a antibióticos |
topic |
Proteus mirabilis ciprofloxacina antioxidantes resistencia a antibióticos |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
This study investigates new aspects of the possible role of antioxidant defenses in the mechanisms of resistance to ciprofloxacin in Proteus mirabilis. Four ciprofloxacin-resistant variants (CRVs), selected in vitro by repeated cultures in a subminimum inhibitory concentration (MIC) concentration of ciprofloxacin, attained different levels of antibiotic resistance and high Ferric reducing antioxidant power, with 10 6 frequencies. However, no mutations occurred in positions 83 or 87 of gyrA, 464 or 466 of gyrB, or 78, 80 or 84 of parC, suggesting that resistance took place without these typical mutations in DNA gyrase or topoisomerase IV. Assays with ciprofloxacin and the pump inhibitor carbonyl cyanide m-chlorophenylhydrazone showed that in addition to the antioxidant mechanisms, the influx/efflux mechanism also contributed to the increase in the resistance to ciprofloxacin in one CRV. Moreover, lipid oxidation to malondialdehyde and protein oxidation to carbonyls and advanced oxidation protein products were higher in sensitive than in the resistant strains, as a new factor involved in the mechanisms of resistance in P. mirabilis. The oxidative stress cross-resistance to telluride in CRVs enhanced the role of the antioxidants in the ciprofloxacin resistance of P. mirabilis, which was reinforced during the assays of reduction of susceptibility to ciprofloxacin by glutathione and ascorbic acid. Fil: Aiassa, Virginia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina Fil: Barnes, Ana Isabel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina Fil: Smania, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina Fil: Albesa, Inés. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina |
description |
This study investigates new aspects of the possible role of antioxidant defenses in the mechanisms of resistance to ciprofloxacin in Proteus mirabilis. Four ciprofloxacin-resistant variants (CRVs), selected in vitro by repeated cultures in a subminimum inhibitory concentration (MIC) concentration of ciprofloxacin, attained different levels of antibiotic resistance and high Ferric reducing antioxidant power, with 10 6 frequencies. However, no mutations occurred in positions 83 or 87 of gyrA, 464 or 466 of gyrB, or 78, 80 or 84 of parC, suggesting that resistance took place without these typical mutations in DNA gyrase or topoisomerase IV. Assays with ciprofloxacin and the pump inhibitor carbonyl cyanide m-chlorophenylhydrazone showed that in addition to the antioxidant mechanisms, the influx/efflux mechanism also contributed to the increase in the resistance to ciprofloxacin in one CRV. Moreover, lipid oxidation to malondialdehyde and protein oxidation to carbonyls and advanced oxidation protein products were higher in sensitive than in the resistant strains, as a new factor involved in the mechanisms of resistance in P. mirabilis. The oxidative stress cross-resistance to telluride in CRVs enhanced the role of the antioxidants in the ciprofloxacin resistance of P. mirabilis, which was reinforced during the assays of reduction of susceptibility to ciprofloxacin by glutathione and ascorbic acid. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/269086 Aiassa, Virginia; Barnes, Ana Isabel; Smania, Andrea; Albesa, Inés; Sublethal ciprofloxacin treatment leads to resistance via antioxidant systems in Proteus mirabilis; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 327; 1; 2-2012; 25-32 0378-1097 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/269086 |
identifier_str_mv |
Aiassa, Virginia; Barnes, Ana Isabel; Smania, Andrea; Albesa, Inés; Sublethal ciprofloxacin treatment leads to resistance via antioxidant systems in Proteus mirabilis; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 327; 1; 2-2012; 25-32 0378-1097 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article-abstract/327/1/25/534190?redirectedFrom=fulltext info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6968.2011.02453.x |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842268914765004800 |
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13.13397 |