Innate immune responses to Proteus mirabilis flagellin in the urinary tract

Autores
Umpiérrez, Analía Alejandra Sofía; Scavone, Paola; Romanin, David Emmanuel; Marqués, Juan Martín; Chabalgoity Rodríguez, José Alejandro; Rumbo, Martín; Zunino, Pablo
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Flagella are bacterial virulence factors allowing microorganisms to move over surfaces. Flagellin, the structural component of flagella, is sensed by the host via Toll and NOD-like receptors and triggers pro-inflammatory responses. The use of Toll-like receptors agonists to modulate innate immune responses has aroused great interest as an alternative to improve the treatment of diverse infectious diseases. Proteus mirabilis is a Gram negative bacterium that causes urinary tract infections in humans. In the present work we used different approaches to study the ability of P. mirabilis flagellin to induce an innate immune response. We demonstrated that P. mirabilis flagellin has the ability to induce pro-inflammatory chemokines expression in T24 bladder cultures cells and in the mouse bladder after instillation. It was evidenced also that flagellin from different P. mirabilis strains differed in their capacity to induce an innate immune response in the CacoCCL20-Luc system. Also, flagellin elicited inflammation, with recruitment of leukocytes to the bladder epithelium. Flagellin instillation before an experimental P. mirabilis infection showed that the inflammatory response due to flagellin did not help to clear the infection but favored bacterial colonization. Thus, induction of inflammatory response in the bladder did not contribute to P. mirabilis infection neutralization
Fil: Umpiérrez, Analía Alejandra Sofía. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Scavone, Paola. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Romanin, David Emmanuel. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marqués, Juan Martín. Universidad de la República; Uruguay
Fil: Chabalgoity Rodríguez, José Alejandro. Universidad de la República; Uruguay
Fil: Rumbo, Martín. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zunino, Pablo. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Materia
Proteus Mirabilis
Uti
Flagellin
Innate Immune Response
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23369
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/23369
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Innate immune responses to Proteus mirabilis flagellin in the urinary tractUmpiérrez, Analía Alejandra SofíaScavone, PaolaRomanin, David EmmanuelMarqués, Juan MartínChabalgoity Rodríguez, José AlejandroRumbo, MartínZunino, PabloProteus MirabilisUtiFlagellinInnate Immune Responsehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Flagella are bacterial virulence factors allowing microorganisms to move over surfaces. Flagellin, the structural component of flagella, is sensed by the host via Toll and NOD-like receptors and triggers pro-inflammatory responses. The use of Toll-like receptors agonists to modulate innate immune responses has aroused great interest as an alternative to improve the treatment of diverse infectious diseases. Proteus mirabilis is a Gram negative bacterium that causes urinary tract infections in humans. In the present work we used different approaches to study the ability of P. mirabilis flagellin to induce an innate immune response. We demonstrated that P. mirabilis flagellin has the ability to induce pro-inflammatory chemokines expression in T24 bladder cultures cells and in the mouse bladder after instillation. It was evidenced also that flagellin from different P. mirabilis strains differed in their capacity to induce an innate immune response in the CacoCCL20-Luc system. Also, flagellin elicited inflammation, with recruitment of leukocytes to the bladder epithelium. Flagellin instillation before an experimental P. mirabilis infection showed that the inflammatory response due to flagellin did not help to clear the infection but favored bacterial colonization. Thus, induction of inflammatory response in the bladder did not contribute to P. mirabilis infection neutralizationFil: Umpiérrez, Analía Alejandra Sofía. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Scavone, Paola. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Romanin, David Emmanuel. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marqués, Juan Martín. Universidad de la República; UruguayFil: Chabalgoity Rodríguez, José Alejandro. Universidad de la República; UruguayFil: Rumbo, Martín. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zunino, Pablo. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayElsevier Masson2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23369Umpiérrez, Analía Alejandra Sofía; Scavone, Paola; Romanin, David Emmanuel; Marqués, Juan Martín; Chabalgoity Rodríguez, José Alejandro; et al.; Innate immune responses to Proteus mirabilis flagellin in the urinary tract; Elsevier Masson; Microbes and Infection; 15; 10-11; 6-2013; 688-6961286-4579CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.micinf.2013.06.007info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1286457913001226info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:43:17Zoai:ri.conicet.gov.ar:11336/23369instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:43:17.836CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Innate immune responses to Proteus mirabilis flagellin in the urinary tract
title Innate immune responses to Proteus mirabilis flagellin in the urinary tract
spellingShingle Innate immune responses to Proteus mirabilis flagellin in the urinary tract
Umpiérrez, Analía Alejandra Sofía
Proteus Mirabilis
Uti
Flagellin
Innate Immune Response
title_short Innate immune responses to Proteus mirabilis flagellin in the urinary tract
title_full Innate immune responses to Proteus mirabilis flagellin in the urinary tract
title_fullStr Innate immune responses to Proteus mirabilis flagellin in the urinary tract
title_full_unstemmed Innate immune responses to Proteus mirabilis flagellin in the urinary tract
title_sort Innate immune responses to Proteus mirabilis flagellin in the urinary tract
dc.creator.none.fl_str_mv Umpiérrez, Analía Alejandra Sofía
Scavone, Paola
Romanin, David Emmanuel
Marqués, Juan Martín
Chabalgoity Rodríguez, José Alejandro
Rumbo, Martín
Zunino, Pablo
author Umpiérrez, Analía Alejandra Sofía
author_facet Umpiérrez, Analía Alejandra Sofía
Scavone, Paola
Romanin, David Emmanuel
Marqués, Juan Martín
Chabalgoity Rodríguez, José Alejandro
Rumbo, Martín
Zunino, Pablo
author_role author
author2 Scavone, Paola
Romanin, David Emmanuel
Marqués, Juan Martín
Chabalgoity Rodríguez, José Alejandro
Rumbo, Martín
Zunino, Pablo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Proteus Mirabilis
Uti
Flagellin
Innate Immune Response
topic Proteus Mirabilis
Uti
Flagellin
Innate Immune Response
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Flagella are bacterial virulence factors allowing microorganisms to move over surfaces. Flagellin, the structural component of flagella, is sensed by the host via Toll and NOD-like receptors and triggers pro-inflammatory responses. The use of Toll-like receptors agonists to modulate innate immune responses has aroused great interest as an alternative to improve the treatment of diverse infectious diseases. Proteus mirabilis is a Gram negative bacterium that causes urinary tract infections in humans. In the present work we used different approaches to study the ability of P. mirabilis flagellin to induce an innate immune response. We demonstrated that P. mirabilis flagellin has the ability to induce pro-inflammatory chemokines expression in T24 bladder cultures cells and in the mouse bladder after instillation. It was evidenced also that flagellin from different P. mirabilis strains differed in their capacity to induce an innate immune response in the CacoCCL20-Luc system. Also, flagellin elicited inflammation, with recruitment of leukocytes to the bladder epithelium. Flagellin instillation before an experimental P. mirabilis infection showed that the inflammatory response due to flagellin did not help to clear the infection but favored bacterial colonization. Thus, induction of inflammatory response in the bladder did not contribute to P. mirabilis infection neutralization
Fil: Umpiérrez, Analía Alejandra Sofía. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Scavone, Paola. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Romanin, David Emmanuel. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marqués, Juan Martín. Universidad de la República; Uruguay
Fil: Chabalgoity Rodríguez, José Alejandro. Universidad de la República; Uruguay
Fil: Rumbo, Martín. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zunino, Pablo. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
description Flagella are bacterial virulence factors allowing microorganisms to move over surfaces. Flagellin, the structural component of flagella, is sensed by the host via Toll and NOD-like receptors and triggers pro-inflammatory responses. The use of Toll-like receptors agonists to modulate innate immune responses has aroused great interest as an alternative to improve the treatment of diverse infectious diseases. Proteus mirabilis is a Gram negative bacterium that causes urinary tract infections in humans. In the present work we used different approaches to study the ability of P. mirabilis flagellin to induce an innate immune response. We demonstrated that P. mirabilis flagellin has the ability to induce pro-inflammatory chemokines expression in T24 bladder cultures cells and in the mouse bladder after instillation. It was evidenced also that flagellin from different P. mirabilis strains differed in their capacity to induce an innate immune response in the CacoCCL20-Luc system. Also, flagellin elicited inflammation, with recruitment of leukocytes to the bladder epithelium. Flagellin instillation before an experimental P. mirabilis infection showed that the inflammatory response due to flagellin did not help to clear the infection but favored bacterial colonization. Thus, induction of inflammatory response in the bladder did not contribute to P. mirabilis infection neutralization
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23369
Umpiérrez, Analía Alejandra Sofía; Scavone, Paola; Romanin, David Emmanuel; Marqués, Juan Martín; Chabalgoity Rodríguez, José Alejandro; et al.; Innate immune responses to Proteus mirabilis flagellin in the urinary tract; Elsevier Masson; Microbes and Infection; 15; 10-11; 6-2013; 688-696
1286-4579
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23369
identifier_str_mv Umpiérrez, Analía Alejandra Sofía; Scavone, Paola; Romanin, David Emmanuel; Marqués, Juan Martín; Chabalgoity Rodríguez, José Alejandro; et al.; Innate immune responses to Proteus mirabilis flagellin in the urinary tract; Elsevier Masson; Microbes and Infection; 15; 10-11; 6-2013; 688-696
1286-4579
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micinf.2013.06.007
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1286457913001226
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Masson
publisher.none.fl_str_mv Elsevier Masson
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614467867901952
score 13.070432

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