A Medicago truncatula rdr6 allele impairs transgene silencing and endogenous phased siRNA production but not development

Autores
Bustos Sanmamed, Maria del Pilar; Hudik, Elodie; Laffont, Carole; Reynes, Christelle; Sallet, Erika; Wen, Jiangqi; Mysore, Kirankumar S.; Camproux, Anne Claude; Hartmann, Caroline; Gouzy, Jérome; Frugier, Florian; Crespi, Martin; Lelandais Brière, Christine
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Summary: RNA-dependent RNA polymerase 6 (RDR6) and suppressor of gene silencing 3 (SGS3) act together in post-transcriptional transgene silencing mediated by small interfering RNAs (siRNAs) and in biogenesis of various endogenous siRNAs including the tasiARFs, known regulators of auxin responses and plant development. Legumes, the third major crop family worldwide, has been widely improved through transgenic approaches. Here, we isolated rdr6 and sgs3 mutants in the model legume Medicago truncatula. Two sgs3 and one rdr6 alleles led to strong developmental defects and impaired biogenesis of tasiARFs. In contrast, the rdr6.1 homozygous plants produced sufficient amounts of tasiARFs to ensure proper development. High throughput sequencing of small RNAs from this specific mutant identified 354 potential MtRDR6 substrates, for which siRNA production was significantly reduced in the mutant. Among them, we found a large variety of novel phased loci corresponding to protein-encoding genes or transposable elements. Interestingly, measurement of GFP expression revealed that post-transcriptional transgene silencing was reduced in rdr6.1 roots. Hence, this novel mis-sense mutation, affecting a highly conserved amino acid residue in plant RDR6s, may be an interesting tool both to analyse endogenous pha-siRNA functions and to improve transgene expression, at least in legume species.
Fil: Bustos Sanmamed, Maria del Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Institut Des Sciences Du Végétal; Francia
Fil: Hudik, Elodie. Institut Des Sciences Du Végétal; Francia
Fil: Laffont, Carole. Institut Des Sciences Du Végétal; Francia
Fil: Reynes, Christelle. Molécules Thérapeutiques In Silico; Francia. Université Paris Diderot - Paris 7; Francia
Fil: Sallet, Erika. Laboratoire Des Interactions Plantes-microorganismes; Francia
Fil: Wen, Jiangqi. The Samuel Roberts Noble Foundation; Estados Unidos
Fil: Mysore, Kirankumar S.. The Samuel Roberts Noble Foundation; Estados Unidos
Fil: Camproux, Anne Claude. Université Paris Diderot - Paris 7; Francia. Molécules Thérapeutiques In Silico; Francia
Fil: Hartmann, Caroline. Institut Des Sciences Du Végétal; Francia. Université Paris Diderot - Paris 7; Francia
Fil: Gouzy, Jérome. Laboratoire Des Interactions Plantes-microorganismes; Francia
Fil: Frugier, Florian. Institut Des Sciences Du Végétal; Francia
Fil: Crespi, Martin. Institut Des Sciences Du Végétal; Francia
Fil: Lelandais Brière, Christine. Institut Des Sciences Du Végétal; Francia. Université Paris Diderot - Paris 7; Francia
Materia
LEGUMES
PHA-SIRNA
RDR6
RNA SILENCING
SGS3
SMALL RNAS
TA-SIRNA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/94271
Acceder
id CONICETDig_7d6770cb24f8323d18bd0ab641ed6587
oai_identifier_str oai:ri.conicet.gov.ar:11336/94271
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A Medicago truncatula rdr6 allele impairs transgene silencing and endogenous phased siRNA production but not developmentBustos Sanmamed, Maria del PilarHudik, ElodieLaffont, CaroleReynes, ChristelleSallet, ErikaWen, JiangqiMysore, Kirankumar S.Camproux, Anne ClaudeHartmann, CarolineGouzy, JéromeFrugier, FlorianCrespi, MartinLelandais Brière, ChristineLEGUMESPHA-SIRNARDR6RNA SILENCINGSGS3SMALL RNASTA-SIRNAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Summary: RNA-dependent RNA polymerase 6 (RDR6) and suppressor of gene silencing 3 (SGS3) act together in post-transcriptional transgene silencing mediated by small interfering RNAs (siRNAs) and in biogenesis of various endogenous siRNAs including the tasiARFs, known regulators of auxin responses and plant development. Legumes, the third major crop family worldwide, has been widely improved through transgenic approaches. Here, we isolated rdr6 and sgs3 mutants in the model legume Medicago truncatula. Two sgs3 and one rdr6 alleles led to strong developmental defects and impaired biogenesis of tasiARFs. In contrast, the rdr6.1 homozygous plants produced sufficient amounts of tasiARFs to ensure proper development. High throughput sequencing of small RNAs from this specific mutant identified 354 potential MtRDR6 substrates, for which siRNA production was significantly reduced in the mutant. Among them, we found a large variety of novel phased loci corresponding to protein-encoding genes or transposable elements. Interestingly, measurement of GFP expression revealed that post-transcriptional transgene silencing was reduced in rdr6.1 roots. Hence, this novel mis-sense mutation, affecting a highly conserved amino acid residue in plant RDR6s, may be an interesting tool both to analyse endogenous pha-siRNA functions and to improve transgene expression, at least in legume species.Fil: Bustos Sanmamed, Maria del Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Institut Des Sciences Du Végétal; FranciaFil: Hudik, Elodie. Institut Des Sciences Du Végétal; FranciaFil: Laffont, Carole. Institut Des Sciences Du Végétal; FranciaFil: Reynes, Christelle. Molécules Thérapeutiques In Silico; Francia. Université Paris Diderot - Paris 7; FranciaFil: Sallet, Erika. Laboratoire Des Interactions Plantes-microorganismes; FranciaFil: Wen, Jiangqi. The Samuel Roberts Noble Foundation; Estados UnidosFil: Mysore, Kirankumar S.. The Samuel Roberts Noble Foundation; Estados UnidosFil: Camproux, Anne Claude. Université Paris Diderot - Paris 7; Francia. Molécules Thérapeutiques In Silico; FranciaFil: Hartmann, Caroline. Institut Des Sciences Du Végétal; Francia. Université Paris Diderot - Paris 7; FranciaFil: Gouzy, Jérome. Laboratoire Des Interactions Plantes-microorganismes; FranciaFil: Frugier, Florian. Institut Des Sciences Du Végétal; FranciaFil: Crespi, Martin. Institut Des Sciences Du Végétal; FranciaFil: Lelandais Brière, Christine. Institut Des Sciences Du Végétal; Francia. Université Paris Diderot - Paris 7; FranciaWiley Blackwell Publishing, Inc2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94271Bustos Sanmamed, Maria del Pilar; Hudik, Elodie; Laffont, Carole; Reynes, Christelle; Sallet, Erika; et al.; A Medicago truncatula rdr6 allele impairs transgene silencing and endogenous phased siRNA production but not development; Wiley Blackwell Publishing, Inc; Plant Biotechnology Journal; 12; 9; 12-2014; 1308-13181467-7644CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/pbi.12230info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/pbi.12230info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:01Zoai:ri.conicet.gov.ar:11336/94271instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:01.394CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A Medicago truncatula rdr6 allele impairs transgene silencing and endogenous phased siRNA production but not development
title A Medicago truncatula rdr6 allele impairs transgene silencing and endogenous phased siRNA production but not development
spellingShingle A Medicago truncatula rdr6 allele impairs transgene silencing and endogenous phased siRNA production but not development
Bustos Sanmamed, Maria del Pilar
LEGUMES
PHA-SIRNA
RDR6
RNA SILENCING
SGS3
SMALL RNAS
TA-SIRNA
title_short A Medicago truncatula rdr6 allele impairs transgene silencing and endogenous phased siRNA production but not development
title_full A Medicago truncatula rdr6 allele impairs transgene silencing and endogenous phased siRNA production but not development
title_fullStr A Medicago truncatula rdr6 allele impairs transgene silencing and endogenous phased siRNA production but not development
title_full_unstemmed A Medicago truncatula rdr6 allele impairs transgene silencing and endogenous phased siRNA production but not development
title_sort A Medicago truncatula rdr6 allele impairs transgene silencing and endogenous phased siRNA production but not development
dc.creator.none.fl_str_mv Bustos Sanmamed, Maria del Pilar
Hudik, Elodie
Laffont, Carole
Reynes, Christelle
Sallet, Erika
Wen, Jiangqi
Mysore, Kirankumar S.
Camproux, Anne Claude
Hartmann, Caroline
Gouzy, Jérome
Frugier, Florian
Crespi, Martin
Lelandais Brière, Christine
author Bustos Sanmamed, Maria del Pilar
author_facet Bustos Sanmamed, Maria del Pilar
Hudik, Elodie
Laffont, Carole
Reynes, Christelle
Sallet, Erika
Wen, Jiangqi
Mysore, Kirankumar S.
Camproux, Anne Claude
Hartmann, Caroline
Gouzy, Jérome
Frugier, Florian
Crespi, Martin
Lelandais Brière, Christine
author_role author
author2 Hudik, Elodie
Laffont, Carole
Reynes, Christelle
Sallet, Erika
Wen, Jiangqi
Mysore, Kirankumar S.
Camproux, Anne Claude
Hartmann, Caroline
Gouzy, Jérome
Frugier, Florian
Crespi, Martin
Lelandais Brière, Christine
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv LEGUMES
PHA-SIRNA
RDR6
RNA SILENCING
SGS3
SMALL RNAS
TA-SIRNA
topic LEGUMES
PHA-SIRNA
RDR6
RNA SILENCING
SGS3
SMALL RNAS
TA-SIRNA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Summary: RNA-dependent RNA polymerase 6 (RDR6) and suppressor of gene silencing 3 (SGS3) act together in post-transcriptional transgene silencing mediated by small interfering RNAs (siRNAs) and in biogenesis of various endogenous siRNAs including the tasiARFs, known regulators of auxin responses and plant development. Legumes, the third major crop family worldwide, has been widely improved through transgenic approaches. Here, we isolated rdr6 and sgs3 mutants in the model legume Medicago truncatula. Two sgs3 and one rdr6 alleles led to strong developmental defects and impaired biogenesis of tasiARFs. In contrast, the rdr6.1 homozygous plants produced sufficient amounts of tasiARFs to ensure proper development. High throughput sequencing of small RNAs from this specific mutant identified 354 potential MtRDR6 substrates, for which siRNA production was significantly reduced in the mutant. Among them, we found a large variety of novel phased loci corresponding to protein-encoding genes or transposable elements. Interestingly, measurement of GFP expression revealed that post-transcriptional transgene silencing was reduced in rdr6.1 roots. Hence, this novel mis-sense mutation, affecting a highly conserved amino acid residue in plant RDR6s, may be an interesting tool both to analyse endogenous pha-siRNA functions and to improve transgene expression, at least in legume species.
Fil: Bustos Sanmamed, Maria del Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Institut Des Sciences Du Végétal; Francia
Fil: Hudik, Elodie. Institut Des Sciences Du Végétal; Francia
Fil: Laffont, Carole. Institut Des Sciences Du Végétal; Francia
Fil: Reynes, Christelle. Molécules Thérapeutiques In Silico; Francia. Université Paris Diderot - Paris 7; Francia
Fil: Sallet, Erika. Laboratoire Des Interactions Plantes-microorganismes; Francia
Fil: Wen, Jiangqi. The Samuel Roberts Noble Foundation; Estados Unidos
Fil: Mysore, Kirankumar S.. The Samuel Roberts Noble Foundation; Estados Unidos
Fil: Camproux, Anne Claude. Université Paris Diderot - Paris 7; Francia. Molécules Thérapeutiques In Silico; Francia
Fil: Hartmann, Caroline. Institut Des Sciences Du Végétal; Francia. Université Paris Diderot - Paris 7; Francia
Fil: Gouzy, Jérome. Laboratoire Des Interactions Plantes-microorganismes; Francia
Fil: Frugier, Florian. Institut Des Sciences Du Végétal; Francia
Fil: Crespi, Martin. Institut Des Sciences Du Végétal; Francia
Fil: Lelandais Brière, Christine. Institut Des Sciences Du Végétal; Francia. Université Paris Diderot - Paris 7; Francia
description Summary: RNA-dependent RNA polymerase 6 (RDR6) and suppressor of gene silencing 3 (SGS3) act together in post-transcriptional transgene silencing mediated by small interfering RNAs (siRNAs) and in biogenesis of various endogenous siRNAs including the tasiARFs, known regulators of auxin responses and plant development. Legumes, the third major crop family worldwide, has been widely improved through transgenic approaches. Here, we isolated rdr6 and sgs3 mutants in the model legume Medicago truncatula. Two sgs3 and one rdr6 alleles led to strong developmental defects and impaired biogenesis of tasiARFs. In contrast, the rdr6.1 homozygous plants produced sufficient amounts of tasiARFs to ensure proper development. High throughput sequencing of small RNAs from this specific mutant identified 354 potential MtRDR6 substrates, for which siRNA production was significantly reduced in the mutant. Among them, we found a large variety of novel phased loci corresponding to protein-encoding genes or transposable elements. Interestingly, measurement of GFP expression revealed that post-transcriptional transgene silencing was reduced in rdr6.1 roots. Hence, this novel mis-sense mutation, affecting a highly conserved amino acid residue in plant RDR6s, may be an interesting tool both to analyse endogenous pha-siRNA functions and to improve transgene expression, at least in legume species.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94271
Bustos Sanmamed, Maria del Pilar; Hudik, Elodie; Laffont, Carole; Reynes, Christelle; Sallet, Erika; et al.; A Medicago truncatula rdr6 allele impairs transgene silencing and endogenous phased siRNA production but not development; Wiley Blackwell Publishing, Inc; Plant Biotechnology Journal; 12; 9; 12-2014; 1308-1318
1467-7644
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94271
identifier_str_mv Bustos Sanmamed, Maria del Pilar; Hudik, Elodie; Laffont, Carole; Reynes, Christelle; Sallet, Erika; et al.; A Medicago truncatula rdr6 allele impairs transgene silencing and endogenous phased siRNA production but not development; Wiley Blackwell Publishing, Inc; Plant Biotechnology Journal; 12; 9; 12-2014; 1308-1318
1467-7644
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/pbi.12230
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/pbi.12230
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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