Multielectrode Recordings From Identified Neurons Involved in Visually Elicited Escape Behavior

Autores
Cámera, Alejandro Gabriel; Belluscio, Mariano Andres; Tomsic, Daniel
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A major challenge in current neuroscience is to understand the concerted functioning of distinct neurons involved in a particular behavior. This goal first requires achieving an adequate characterization of the behavior as well as an identification of the key neuronal elements associated with that action. Such conditions have been considerably attained for the escape response to visual stimuli in the crab Neohelice. During the last two decades a combination of in vivo intracellular recordings and staining with behavioral experiments and modeling, led us to postulate that a microcircuit formed by four classes of identified lobula giant (LG) neurons operates as a decision-making node for several important visually-guided components of the crab’s escape behavior. However, these studies were done by recording LG neurons individually. To investigate the combined operations performed by the group of LG neurons, we began to use multielectrode recordings. Here we describe the methodology and show results of simultaneously recorded activity from different lobula elements. The different LG classes can be distinguished by their differential responses to particular visual stimuli. By comparing the response profiles of extracellular recorded units with intracellular recorded responses to the same stimuli, two of the four LG classes could be faithfully recognized. Additionally, we recorded units with stimulus preferences different from those exhibited by the LG neurons. Among these, we found units sensitive to optic flow with marked directional preference. Units classified within a single group according to their response profiles exhibited similar spike waveforms and similar auto-correlograms, but which, on the other hand, differed from those of groups with different response profiles. Additionally, cross-correlograms revealed excitatory as well as inhibitory relationships between recognizable units. Thus, the extracellular multielectrode methodology allowed us to stably record from previously identified neurons as well as from undescribed elements of the brain of the crab. Moreover, simultaneous multiunit recording allowed beginning to disclose the connections between central elements of the visual circuits. This work provides an entry point into studying the neural networks underlying the control of visually guided behaviors in the crab brain.
Fil: Cámera, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Belluscio, Mariano Andres. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tomsic, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Materia
AVOIDANCE
CRUSTACEAN
GIANT NEURONS
INSECT
MOTION DETECTION
SIMULTANEOUS EXTRACELLULAR RECORDING
TETRODES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/141623

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spelling Multielectrode Recordings From Identified Neurons Involved in Visually Elicited Escape BehaviorCámera, Alejandro GabrielBelluscio, Mariano AndresTomsic, DanielAVOIDANCECRUSTACEANGIANT NEURONSINSECTMOTION DETECTIONSIMULTANEOUS EXTRACELLULAR RECORDINGTETRODEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A major challenge in current neuroscience is to understand the concerted functioning of distinct neurons involved in a particular behavior. This goal first requires achieving an adequate characterization of the behavior as well as an identification of the key neuronal elements associated with that action. Such conditions have been considerably attained for the escape response to visual stimuli in the crab Neohelice. During the last two decades a combination of in vivo intracellular recordings and staining with behavioral experiments and modeling, led us to postulate that a microcircuit formed by four classes of identified lobula giant (LG) neurons operates as a decision-making node for several important visually-guided components of the crab’s escape behavior. However, these studies were done by recording LG neurons individually. To investigate the combined operations performed by the group of LG neurons, we began to use multielectrode recordings. Here we describe the methodology and show results of simultaneously recorded activity from different lobula elements. The different LG classes can be distinguished by their differential responses to particular visual stimuli. By comparing the response profiles of extracellular recorded units with intracellular recorded responses to the same stimuli, two of the four LG classes could be faithfully recognized. Additionally, we recorded units with stimulus preferences different from those exhibited by the LG neurons. Among these, we found units sensitive to optic flow with marked directional preference. Units classified within a single group according to their response profiles exhibited similar spike waveforms and similar auto-correlograms, but which, on the other hand, differed from those of groups with different response profiles. Additionally, cross-correlograms revealed excitatory as well as inhibitory relationships between recognizable units. Thus, the extracellular multielectrode methodology allowed us to stably record from previously identified neurons as well as from undescribed elements of the brain of the crab. Moreover, simultaneous multiunit recording allowed beginning to disclose the connections between central elements of the visual circuits. This work provides an entry point into studying the neural networks underlying the control of visually guided behaviors in the crab brain.Fil: Cámera, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Belluscio, Mariano Andres. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tomsic, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFrontiers Media2020-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/141623Cámera, Alejandro Gabriel; Belluscio, Mariano Andres; Tomsic, Daniel; Multielectrode Recordings From Identified Neurons Involved in Visually Elicited Escape Behavior; Frontiers Media; Frontiers in Behavioral Neuroscience; 14; 11-2020; 1-151662-5153CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fnbeh.2020.592309/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fnbeh.2020.592309info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:25:23Zoai:ri.conicet.gov.ar:11336/141623instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:25:23.943CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Multielectrode Recordings From Identified Neurons Involved in Visually Elicited Escape Behavior
title Multielectrode Recordings From Identified Neurons Involved in Visually Elicited Escape Behavior
spellingShingle Multielectrode Recordings From Identified Neurons Involved in Visually Elicited Escape Behavior
Cámera, Alejandro Gabriel
AVOIDANCE
CRUSTACEAN
GIANT NEURONS
INSECT
MOTION DETECTION
SIMULTANEOUS EXTRACELLULAR RECORDING
TETRODES
title_short Multielectrode Recordings From Identified Neurons Involved in Visually Elicited Escape Behavior
title_full Multielectrode Recordings From Identified Neurons Involved in Visually Elicited Escape Behavior
title_fullStr Multielectrode Recordings From Identified Neurons Involved in Visually Elicited Escape Behavior
title_full_unstemmed Multielectrode Recordings From Identified Neurons Involved in Visually Elicited Escape Behavior
title_sort Multielectrode Recordings From Identified Neurons Involved in Visually Elicited Escape Behavior
dc.creator.none.fl_str_mv Cámera, Alejandro Gabriel
Belluscio, Mariano Andres
Tomsic, Daniel
author Cámera, Alejandro Gabriel
author_facet Cámera, Alejandro Gabriel
Belluscio, Mariano Andres
Tomsic, Daniel
author_role author
author2 Belluscio, Mariano Andres
Tomsic, Daniel
author2_role author
author
dc.subject.none.fl_str_mv AVOIDANCE
CRUSTACEAN
GIANT NEURONS
INSECT
MOTION DETECTION
SIMULTANEOUS EXTRACELLULAR RECORDING
TETRODES
topic AVOIDANCE
CRUSTACEAN
GIANT NEURONS
INSECT
MOTION DETECTION
SIMULTANEOUS EXTRACELLULAR RECORDING
TETRODES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A major challenge in current neuroscience is to understand the concerted functioning of distinct neurons involved in a particular behavior. This goal first requires achieving an adequate characterization of the behavior as well as an identification of the key neuronal elements associated with that action. Such conditions have been considerably attained for the escape response to visual stimuli in the crab Neohelice. During the last two decades a combination of in vivo intracellular recordings and staining with behavioral experiments and modeling, led us to postulate that a microcircuit formed by four classes of identified lobula giant (LG) neurons operates as a decision-making node for several important visually-guided components of the crab’s escape behavior. However, these studies were done by recording LG neurons individually. To investigate the combined operations performed by the group of LG neurons, we began to use multielectrode recordings. Here we describe the methodology and show results of simultaneously recorded activity from different lobula elements. The different LG classes can be distinguished by their differential responses to particular visual stimuli. By comparing the response profiles of extracellular recorded units with intracellular recorded responses to the same stimuli, two of the four LG classes could be faithfully recognized. Additionally, we recorded units with stimulus preferences different from those exhibited by the LG neurons. Among these, we found units sensitive to optic flow with marked directional preference. Units classified within a single group according to their response profiles exhibited similar spike waveforms and similar auto-correlograms, but which, on the other hand, differed from those of groups with different response profiles. Additionally, cross-correlograms revealed excitatory as well as inhibitory relationships between recognizable units. Thus, the extracellular multielectrode methodology allowed us to stably record from previously identified neurons as well as from undescribed elements of the brain of the crab. Moreover, simultaneous multiunit recording allowed beginning to disclose the connections between central elements of the visual circuits. This work provides an entry point into studying the neural networks underlying the control of visually guided behaviors in the crab brain.
Fil: Cámera, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Belluscio, Mariano Andres. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tomsic, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
description A major challenge in current neuroscience is to understand the concerted functioning of distinct neurons involved in a particular behavior. This goal first requires achieving an adequate characterization of the behavior as well as an identification of the key neuronal elements associated with that action. Such conditions have been considerably attained for the escape response to visual stimuli in the crab Neohelice. During the last two decades a combination of in vivo intracellular recordings and staining with behavioral experiments and modeling, led us to postulate that a microcircuit formed by four classes of identified lobula giant (LG) neurons operates as a decision-making node for several important visually-guided components of the crab’s escape behavior. However, these studies were done by recording LG neurons individually. To investigate the combined operations performed by the group of LG neurons, we began to use multielectrode recordings. Here we describe the methodology and show results of simultaneously recorded activity from different lobula elements. The different LG classes can be distinguished by their differential responses to particular visual stimuli. By comparing the response profiles of extracellular recorded units with intracellular recorded responses to the same stimuli, two of the four LG classes could be faithfully recognized. Additionally, we recorded units with stimulus preferences different from those exhibited by the LG neurons. Among these, we found units sensitive to optic flow with marked directional preference. Units classified within a single group according to their response profiles exhibited similar spike waveforms and similar auto-correlograms, but which, on the other hand, differed from those of groups with different response profiles. Additionally, cross-correlograms revealed excitatory as well as inhibitory relationships between recognizable units. Thus, the extracellular multielectrode methodology allowed us to stably record from previously identified neurons as well as from undescribed elements of the brain of the crab. Moreover, simultaneous multiunit recording allowed beginning to disclose the connections between central elements of the visual circuits. This work provides an entry point into studying the neural networks underlying the control of visually guided behaviors in the crab brain.
publishDate 2020
dc.date.none.fl_str_mv 2020-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/141623
Cámera, Alejandro Gabriel; Belluscio, Mariano Andres; Tomsic, Daniel; Multielectrode Recordings From Identified Neurons Involved in Visually Elicited Escape Behavior; Frontiers Media; Frontiers in Behavioral Neuroscience; 14; 11-2020; 1-15
1662-5153
CONICET Digital
CONICET
url http://hdl.handle.net/11336/141623
identifier_str_mv Cámera, Alejandro Gabriel; Belluscio, Mariano Andres; Tomsic, Daniel; Multielectrode Recordings From Identified Neurons Involved in Visually Elicited Escape Behavior; Frontiers Media; Frontiers in Behavioral Neuroscience; 14; 11-2020; 1-15
1662-5153
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.3389/fnbeh.2020.592309
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
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application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
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