Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae)

Autores
Genovese, Griselda; Regueira, Mariana; Da Cuña, Rodrigo Hernán; Ferreira, Maria Florencia; Varela, María Luisa; Lo Nostro, Fabiana Laura
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In oviparous vertebrates, vitellogenin (VTG) is mainly produced by the liver in response to estrogen(E2) and its synthesis is traditionally coupled to estrogen receptor alpha induction. Even though VTG isa female-specific protein, chemicals that mimic natural estrogens, known as xenoestrogens, can acti-vate its expression in males causing endocrine disruption to wildlife and humans. Alkylphenols such asnonylphenol (NP) and octylphenol (OP) are industrial additives used in the manufacture of a wide vari-ety of plastics and detergents, and can disrupt endocrine functions in exposed animals. For more than adecade, the freshwater cichlid fish Cichlasoma dimerus has been used for ecotoxicological studies in ourlaboratory. We recently found an up-regulation of VTG gene expression in livers of male fish exposed toOP, from a silent state to values similar to those of E2-induced fish. To better understand the underlyingmechanisms behind the action of xenoestrogens, the aim of this study was to analyze the dose?responserelationship of C. dimerus VTG and estrogen receptors (ERs) gene expression after waterborne exposureto 0.15, 1.5, 15, and 150 g/L OP for up to 1 month (0, 1, 3, 7, 14, 21, and 28 days). At the end of theexperiment, histological features of exposed fish included active hepatocytes with basophilic cytoplasmand high eosinophilic content in their vascular system due to augmented expression of VTG. In testis,high preponderance of sperm was found in fish exposed to 150 g/L OP. A classic dose?response down-regulation of the expression of Na+/K+-ATPase, a ?non-gender specific gene? used for comparison, wasfound with increasing OP concentrations. No VTG and very low levels of ER were detected in controlmale livers, but an up-regulation of both genes was found in males exposed to 0.15 or 150 g/L OP. More-over, VTG transcripts were significant as early as day 3 or day 1 of exposure to these OP concentrations,respectively. Nearly no response was detected in 1.5 and 15 g/L OP exposed-fish. Data was curve-fittedevidencing a nonmonotonic dose?response curve. Interestingly, ER2 mRNA expression was augmentedabove baseline levels only when males were exposed to the lowest OP concentration. We speculate thatgenomic control of vitellogenesis is under control of multiple steroid receptors with different affinitiesfor ligands. ER isoform, only up-regulated with very low concentrations of ligand, would act as a sen-sors of OP (or E2) to induce ER and VTG. With high OP concentrations, the expression of ER isoform ispromptly augmented, with the concomitant VTG transactivation.
Fil: Genovese, Griselda. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Regueira, Mariana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Da Cuña, Rodrigo Hernán. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Ferreira, Maria Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Varela, María Luisa. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lo Nostro, Fabiana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina
Materia
Cichlid Fish
Vitellogenin Gene Expression
Estrogen Receptors
Octylphenol
Nonmonotonic Dose-Response
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32169

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network_name_str CONICET Digital (CONICET)
spelling Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae)Genovese, GriseldaRegueira, MarianaDa Cuña, Rodrigo HernánFerreira, Maria FlorenciaVarela, María LuisaLo Nostro, Fabiana LauraCichlid FishVitellogenin Gene ExpressionEstrogen ReceptorsOctylphenolNonmonotonic Dose-Responsehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In oviparous vertebrates, vitellogenin (VTG) is mainly produced by the liver in response to estrogen(E2) and its synthesis is traditionally coupled to estrogen receptor alpha induction. Even though VTG isa female-specific protein, chemicals that mimic natural estrogens, known as xenoestrogens, can acti-vate its expression in males causing endocrine disruption to wildlife and humans. Alkylphenols such asnonylphenol (NP) and octylphenol (OP) are industrial additives used in the manufacture of a wide vari-ety of plastics and detergents, and can disrupt endocrine functions in exposed animals. For more than adecade, the freshwater cichlid fish Cichlasoma dimerus has been used for ecotoxicological studies in ourlaboratory. We recently found an up-regulation of VTG gene expression in livers of male fish exposed toOP, from a silent state to values similar to those of E2-induced fish. To better understand the underlyingmechanisms behind the action of xenoestrogens, the aim of this study was to analyze the dose?responserelationship of C. dimerus VTG and estrogen receptors (ERs) gene expression after waterborne exposureto 0.15, 1.5, 15, and 150 g/L OP for up to 1 month (0, 1, 3, 7, 14, 21, and 28 days). At the end of theexperiment, histological features of exposed fish included active hepatocytes with basophilic cytoplasmand high eosinophilic content in their vascular system due to augmented expression of VTG. In testis,high preponderance of sperm was found in fish exposed to 150 g/L OP. A classic dose?response down-regulation of the expression of Na+/K+-ATPase, a ?non-gender specific gene? used for comparison, wasfound with increasing OP concentrations. No VTG and very low levels of ER were detected in controlmale livers, but an up-regulation of both genes was found in males exposed to 0.15 or 150 g/L OP. More-over, VTG transcripts were significant as early as day 3 or day 1 of exposure to these OP concentrations,respectively. Nearly no response was detected in 1.5 and 15 g/L OP exposed-fish. Data was curve-fittedevidencing a nonmonotonic dose?response curve. Interestingly, ER2 mRNA expression was augmentedabove baseline levels only when males were exposed to the lowest OP concentration. We speculate thatgenomic control of vitellogenesis is under control of multiple steroid receptors with different affinitiesfor ligands. ER isoform, only up-regulated with very low concentrations of ligand, would act as a sen-sors of OP (or E2) to induce ER and VTG. With high OP concentrations, the expression of ER isoform ispromptly augmented, with the concomitant VTG transactivation.Fil: Genovese, Griselda. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Regueira, Mariana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Da Cuña, Rodrigo Hernán. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Ferreira, Maria Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Varela, María Luisa. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lo Nostro, Fabiana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; ArgentinaElsevier Science2014-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32169Lo Nostro, Fabiana Laura; Varela, María Luisa; Ferreira, Maria Florencia; Da Cuña, Rodrigo Hernán; Regueira, Mariana; Genovese, Griselda; et al.; Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae); Elsevier Science; Aquatic Toxicology; 156; 8-2014; 30-400166-445XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2014.07.019info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0166445X14002550info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:34Zoai:ri.conicet.gov.ar:11336/32169instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:34.827CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae)
title Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae)
spellingShingle Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae)
Genovese, Griselda
Cichlid Fish
Vitellogenin Gene Expression
Estrogen Receptors
Octylphenol
Nonmonotonic Dose-Response
title_short Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae)
title_full Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae)
title_fullStr Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae)
title_full_unstemmed Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae)
title_sort Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae)
dc.creator.none.fl_str_mv Genovese, Griselda
Regueira, Mariana
Da Cuña, Rodrigo Hernán
Ferreira, Maria Florencia
Varela, María Luisa
Lo Nostro, Fabiana Laura
author Genovese, Griselda
author_facet Genovese, Griselda
Regueira, Mariana
Da Cuña, Rodrigo Hernán
Ferreira, Maria Florencia
Varela, María Luisa
Lo Nostro, Fabiana Laura
author_role author
author2 Regueira, Mariana
Da Cuña, Rodrigo Hernán
Ferreira, Maria Florencia
Varela, María Luisa
Lo Nostro, Fabiana Laura
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cichlid Fish
Vitellogenin Gene Expression
Estrogen Receptors
Octylphenol
Nonmonotonic Dose-Response
topic Cichlid Fish
Vitellogenin Gene Expression
Estrogen Receptors
Octylphenol
Nonmonotonic Dose-Response
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In oviparous vertebrates, vitellogenin (VTG) is mainly produced by the liver in response to estrogen(E2) and its synthesis is traditionally coupled to estrogen receptor alpha induction. Even though VTG isa female-specific protein, chemicals that mimic natural estrogens, known as xenoestrogens, can acti-vate its expression in males causing endocrine disruption to wildlife and humans. Alkylphenols such asnonylphenol (NP) and octylphenol (OP) are industrial additives used in the manufacture of a wide vari-ety of plastics and detergents, and can disrupt endocrine functions in exposed animals. For more than adecade, the freshwater cichlid fish Cichlasoma dimerus has been used for ecotoxicological studies in ourlaboratory. We recently found an up-regulation of VTG gene expression in livers of male fish exposed toOP, from a silent state to values similar to those of E2-induced fish. To better understand the underlyingmechanisms behind the action of xenoestrogens, the aim of this study was to analyze the dose?responserelationship of C. dimerus VTG and estrogen receptors (ERs) gene expression after waterborne exposureto 0.15, 1.5, 15, and 150 g/L OP for up to 1 month (0, 1, 3, 7, 14, 21, and 28 days). At the end of theexperiment, histological features of exposed fish included active hepatocytes with basophilic cytoplasmand high eosinophilic content in their vascular system due to augmented expression of VTG. In testis,high preponderance of sperm was found in fish exposed to 150 g/L OP. A classic dose?response down-regulation of the expression of Na+/K+-ATPase, a ?non-gender specific gene? used for comparison, wasfound with increasing OP concentrations. No VTG and very low levels of ER were detected in controlmale livers, but an up-regulation of both genes was found in males exposed to 0.15 or 150 g/L OP. More-over, VTG transcripts were significant as early as day 3 or day 1 of exposure to these OP concentrations,respectively. Nearly no response was detected in 1.5 and 15 g/L OP exposed-fish. Data was curve-fittedevidencing a nonmonotonic dose?response curve. Interestingly, ER2 mRNA expression was augmentedabove baseline levels only when males were exposed to the lowest OP concentration. We speculate thatgenomic control of vitellogenesis is under control of multiple steroid receptors with different affinitiesfor ligands. ER isoform, only up-regulated with very low concentrations of ligand, would act as a sen-sors of OP (or E2) to induce ER and VTG. With high OP concentrations, the expression of ER isoform ispromptly augmented, with the concomitant VTG transactivation.
Fil: Genovese, Griselda. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Regueira, Mariana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Da Cuña, Rodrigo Hernán. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Ferreira, Maria Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Varela, María Luisa. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lo Nostro, Fabiana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina
description In oviparous vertebrates, vitellogenin (VTG) is mainly produced by the liver in response to estrogen(E2) and its synthesis is traditionally coupled to estrogen receptor alpha induction. Even though VTG isa female-specific protein, chemicals that mimic natural estrogens, known as xenoestrogens, can acti-vate its expression in males causing endocrine disruption to wildlife and humans. Alkylphenols such asnonylphenol (NP) and octylphenol (OP) are industrial additives used in the manufacture of a wide vari-ety of plastics and detergents, and can disrupt endocrine functions in exposed animals. For more than adecade, the freshwater cichlid fish Cichlasoma dimerus has been used for ecotoxicological studies in ourlaboratory. We recently found an up-regulation of VTG gene expression in livers of male fish exposed toOP, from a silent state to values similar to those of E2-induced fish. To better understand the underlyingmechanisms behind the action of xenoestrogens, the aim of this study was to analyze the dose?responserelationship of C. dimerus VTG and estrogen receptors (ERs) gene expression after waterborne exposureto 0.15, 1.5, 15, and 150 g/L OP for up to 1 month (0, 1, 3, 7, 14, 21, and 28 days). At the end of theexperiment, histological features of exposed fish included active hepatocytes with basophilic cytoplasmand high eosinophilic content in their vascular system due to augmented expression of VTG. In testis,high preponderance of sperm was found in fish exposed to 150 g/L OP. A classic dose?response down-regulation of the expression of Na+/K+-ATPase, a ?non-gender specific gene? used for comparison, wasfound with increasing OP concentrations. No VTG and very low levels of ER were detected in controlmale livers, but an up-regulation of both genes was found in males exposed to 0.15 or 150 g/L OP. More-over, VTG transcripts were significant as early as day 3 or day 1 of exposure to these OP concentrations,respectively. Nearly no response was detected in 1.5 and 15 g/L OP exposed-fish. Data was curve-fittedevidencing a nonmonotonic dose?response curve. Interestingly, ER2 mRNA expression was augmentedabove baseline levels only when males were exposed to the lowest OP concentration. We speculate thatgenomic control of vitellogenesis is under control of multiple steroid receptors with different affinitiesfor ligands. ER isoform, only up-regulated with very low concentrations of ligand, would act as a sen-sors of OP (or E2) to induce ER and VTG. With high OP concentrations, the expression of ER isoform ispromptly augmented, with the concomitant VTG transactivation.
publishDate 2014
dc.date.none.fl_str_mv 2014-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32169
Lo Nostro, Fabiana Laura; Varela, María Luisa; Ferreira, Maria Florencia; Da Cuña, Rodrigo Hernán; Regueira, Mariana; Genovese, Griselda; et al.; Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae); Elsevier Science; Aquatic Toxicology; 156; 8-2014; 30-40
0166-445X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32169
identifier_str_mv Lo Nostro, Fabiana Laura; Varela, María Luisa; Ferreira, Maria Florencia; Da Cuña, Rodrigo Hernán; Regueira, Mariana; Genovese, Griselda; et al.; Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae); Elsevier Science; Aquatic Toxicology; 156; 8-2014; 30-40
0166-445X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0166445X14002550
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dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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