Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae)
- Autores
- Genovese, Griselda; Regueira, Mariana; Da Cuña, Rodrigo Hernán; Ferreira, Maria Florencia; Varela, María Luisa; Lo Nostro, Fabiana Laura
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In oviparous vertebrates, vitellogenin (VTG) is mainly produced by the liver in response to estrogen(E2) and its synthesis is traditionally coupled to estrogen receptor alpha induction. Even though VTG isa female-specific protein, chemicals that mimic natural estrogens, known as xenoestrogens, can acti-vate its expression in males causing endocrine disruption to wildlife and humans. Alkylphenols such asnonylphenol (NP) and octylphenol (OP) are industrial additives used in the manufacture of a wide vari-ety of plastics and detergents, and can disrupt endocrine functions in exposed animals. For more than adecade, the freshwater cichlid fish Cichlasoma dimerus has been used for ecotoxicological studies in ourlaboratory. We recently found an up-regulation of VTG gene expression in livers of male fish exposed toOP, from a silent state to values similar to those of E2-induced fish. To better understand the underlyingmechanisms behind the action of xenoestrogens, the aim of this study was to analyze the dose?responserelationship of C. dimerus VTG and estrogen receptors (ERs) gene expression after waterborne exposureto 0.15, 1.5, 15, and 150 g/L OP for up to 1 month (0, 1, 3, 7, 14, 21, and 28 days). At the end of theexperiment, histological features of exposed fish included active hepatocytes with basophilic cytoplasmand high eosinophilic content in their vascular system due to augmented expression of VTG. In testis,high preponderance of sperm was found in fish exposed to 150 g/L OP. A classic dose?response down-regulation of the expression of Na+/K+-ATPase, a ?non-gender specific gene? used for comparison, wasfound with increasing OP concentrations. No VTG and very low levels of ER were detected in controlmale livers, but an up-regulation of both genes was found in males exposed to 0.15 or 150 g/L OP. More-over, VTG transcripts were significant as early as day 3 or day 1 of exposure to these OP concentrations,respectively. Nearly no response was detected in 1.5 and 15 g/L OP exposed-fish. Data was curve-fittedevidencing a nonmonotonic dose?response curve. Interestingly, ER2 mRNA expression was augmentedabove baseline levels only when males were exposed to the lowest OP concentration. We speculate thatgenomic control of vitellogenesis is under control of multiple steroid receptors with different affinitiesfor ligands. ER isoform, only up-regulated with very low concentrations of ligand, would act as a sen-sors of OP (or E2) to induce ER and VTG. With high OP concentrations, the expression of ER isoform ispromptly augmented, with the concomitant VTG transactivation.
Fil: Genovese, Griselda. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Regueira, Mariana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Da Cuña, Rodrigo Hernán. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Ferreira, Maria Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Varela, María Luisa. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lo Nostro, Fabiana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina - Materia
-
Cichlid Fish
Vitellogenin Gene Expression
Estrogen Receptors
Octylphenol
Nonmonotonic Dose-Response - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/32169
Ver los metadatos del registro completo
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Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae)Genovese, GriseldaRegueira, MarianaDa Cuña, Rodrigo HernánFerreira, Maria FlorenciaVarela, María LuisaLo Nostro, Fabiana LauraCichlid FishVitellogenin Gene ExpressionEstrogen ReceptorsOctylphenolNonmonotonic Dose-Responsehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In oviparous vertebrates, vitellogenin (VTG) is mainly produced by the liver in response to estrogen(E2) and its synthesis is traditionally coupled to estrogen receptor alpha induction. Even though VTG isa female-specific protein, chemicals that mimic natural estrogens, known as xenoestrogens, can acti-vate its expression in males causing endocrine disruption to wildlife and humans. Alkylphenols such asnonylphenol (NP) and octylphenol (OP) are industrial additives used in the manufacture of a wide vari-ety of plastics and detergents, and can disrupt endocrine functions in exposed animals. For more than adecade, the freshwater cichlid fish Cichlasoma dimerus has been used for ecotoxicological studies in ourlaboratory. We recently found an up-regulation of VTG gene expression in livers of male fish exposed toOP, from a silent state to values similar to those of E2-induced fish. To better understand the underlyingmechanisms behind the action of xenoestrogens, the aim of this study was to analyze the dose?responserelationship of C. dimerus VTG and estrogen receptors (ERs) gene expression after waterborne exposureto 0.15, 1.5, 15, and 150 g/L OP for up to 1 month (0, 1, 3, 7, 14, 21, and 28 days). At the end of theexperiment, histological features of exposed fish included active hepatocytes with basophilic cytoplasmand high eosinophilic content in their vascular system due to augmented expression of VTG. In testis,high preponderance of sperm was found in fish exposed to 150 g/L OP. A classic dose?response down-regulation of the expression of Na+/K+-ATPase, a ?non-gender specific gene? used for comparison, wasfound with increasing OP concentrations. No VTG and very low levels of ER were detected in controlmale livers, but an up-regulation of both genes was found in males exposed to 0.15 or 150 g/L OP. More-over, VTG transcripts were significant as early as day 3 or day 1 of exposure to these OP concentrations,respectively. Nearly no response was detected in 1.5 and 15 g/L OP exposed-fish. Data was curve-fittedevidencing a nonmonotonic dose?response curve. Interestingly, ER2 mRNA expression was augmentedabove baseline levels only when males were exposed to the lowest OP concentration. We speculate thatgenomic control of vitellogenesis is under control of multiple steroid receptors with different affinitiesfor ligands. ER isoform, only up-regulated with very low concentrations of ligand, would act as a sen-sors of OP (or E2) to induce ER and VTG. With high OP concentrations, the expression of ER isoform ispromptly augmented, with the concomitant VTG transactivation.Fil: Genovese, Griselda. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Regueira, Mariana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Da Cuña, Rodrigo Hernán. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Ferreira, Maria Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Varela, María Luisa. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lo Nostro, Fabiana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; ArgentinaElsevier Science2014-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32169Lo Nostro, Fabiana Laura; Varela, María Luisa; Ferreira, Maria Florencia; Da Cuña, Rodrigo Hernán; Regueira, Mariana; Genovese, Griselda; et al.; Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae); Elsevier Science; Aquatic Toxicology; 156; 8-2014; 30-400166-445XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2014.07.019info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0166445X14002550info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:34Zoai:ri.conicet.gov.ar:11336/32169instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:34.827CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae) |
title |
Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae) |
spellingShingle |
Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae) Genovese, Griselda Cichlid Fish Vitellogenin Gene Expression Estrogen Receptors Octylphenol Nonmonotonic Dose-Response |
title_short |
Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae) |
title_full |
Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae) |
title_fullStr |
Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae) |
title_full_unstemmed |
Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae) |
title_sort |
Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae) |
dc.creator.none.fl_str_mv |
Genovese, Griselda Regueira, Mariana Da Cuña, Rodrigo Hernán Ferreira, Maria Florencia Varela, María Luisa Lo Nostro, Fabiana Laura |
author |
Genovese, Griselda |
author_facet |
Genovese, Griselda Regueira, Mariana Da Cuña, Rodrigo Hernán Ferreira, Maria Florencia Varela, María Luisa Lo Nostro, Fabiana Laura |
author_role |
author |
author2 |
Regueira, Mariana Da Cuña, Rodrigo Hernán Ferreira, Maria Florencia Varela, María Luisa Lo Nostro, Fabiana Laura |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Cichlid Fish Vitellogenin Gene Expression Estrogen Receptors Octylphenol Nonmonotonic Dose-Response |
topic |
Cichlid Fish Vitellogenin Gene Expression Estrogen Receptors Octylphenol Nonmonotonic Dose-Response |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
In oviparous vertebrates, vitellogenin (VTG) is mainly produced by the liver in response to estrogen(E2) and its synthesis is traditionally coupled to estrogen receptor alpha induction. Even though VTG isa female-specific protein, chemicals that mimic natural estrogens, known as xenoestrogens, can acti-vate its expression in males causing endocrine disruption to wildlife and humans. Alkylphenols such asnonylphenol (NP) and octylphenol (OP) are industrial additives used in the manufacture of a wide vari-ety of plastics and detergents, and can disrupt endocrine functions in exposed animals. For more than adecade, the freshwater cichlid fish Cichlasoma dimerus has been used for ecotoxicological studies in ourlaboratory. We recently found an up-regulation of VTG gene expression in livers of male fish exposed toOP, from a silent state to values similar to those of E2-induced fish. To better understand the underlyingmechanisms behind the action of xenoestrogens, the aim of this study was to analyze the dose?responserelationship of C. dimerus VTG and estrogen receptors (ERs) gene expression after waterborne exposureto 0.15, 1.5, 15, and 150 g/L OP for up to 1 month (0, 1, 3, 7, 14, 21, and 28 days). At the end of theexperiment, histological features of exposed fish included active hepatocytes with basophilic cytoplasmand high eosinophilic content in their vascular system due to augmented expression of VTG. In testis,high preponderance of sperm was found in fish exposed to 150 g/L OP. A classic dose?response down-regulation of the expression of Na+/K+-ATPase, a ?non-gender specific gene? used for comparison, wasfound with increasing OP concentrations. No VTG and very low levels of ER were detected in controlmale livers, but an up-regulation of both genes was found in males exposed to 0.15 or 150 g/L OP. More-over, VTG transcripts were significant as early as day 3 or day 1 of exposure to these OP concentrations,respectively. Nearly no response was detected in 1.5 and 15 g/L OP exposed-fish. Data was curve-fittedevidencing a nonmonotonic dose?response curve. Interestingly, ER2 mRNA expression was augmentedabove baseline levels only when males were exposed to the lowest OP concentration. We speculate thatgenomic control of vitellogenesis is under control of multiple steroid receptors with different affinitiesfor ligands. ER isoform, only up-regulated with very low concentrations of ligand, would act as a sen-sors of OP (or E2) to induce ER and VTG. With high OP concentrations, the expression of ER isoform ispromptly augmented, with the concomitant VTG transactivation. Fil: Genovese, Griselda. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina Fil: Regueira, Mariana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Da Cuña, Rodrigo Hernán. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina Fil: Ferreira, Maria Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Varela, María Luisa. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lo Nostro, Fabiana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Ecotoxicología Acuática; Argentina |
description |
In oviparous vertebrates, vitellogenin (VTG) is mainly produced by the liver in response to estrogen(E2) and its synthesis is traditionally coupled to estrogen receptor alpha induction. Even though VTG isa female-specific protein, chemicals that mimic natural estrogens, known as xenoestrogens, can acti-vate its expression in males causing endocrine disruption to wildlife and humans. Alkylphenols such asnonylphenol (NP) and octylphenol (OP) are industrial additives used in the manufacture of a wide vari-ety of plastics and detergents, and can disrupt endocrine functions in exposed animals. For more than adecade, the freshwater cichlid fish Cichlasoma dimerus has been used for ecotoxicological studies in ourlaboratory. We recently found an up-regulation of VTG gene expression in livers of male fish exposed toOP, from a silent state to values similar to those of E2-induced fish. To better understand the underlyingmechanisms behind the action of xenoestrogens, the aim of this study was to analyze the dose?responserelationship of C. dimerus VTG and estrogen receptors (ERs) gene expression after waterborne exposureto 0.15, 1.5, 15, and 150 g/L OP for up to 1 month (0, 1, 3, 7, 14, 21, and 28 days). At the end of theexperiment, histological features of exposed fish included active hepatocytes with basophilic cytoplasmand high eosinophilic content in their vascular system due to augmented expression of VTG. In testis,high preponderance of sperm was found in fish exposed to 150 g/L OP. A classic dose?response down-regulation of the expression of Na+/K+-ATPase, a ?non-gender specific gene? used for comparison, wasfound with increasing OP concentrations. No VTG and very low levels of ER were detected in controlmale livers, but an up-regulation of both genes was found in males exposed to 0.15 or 150 g/L OP. More-over, VTG transcripts were significant as early as day 3 or day 1 of exposure to these OP concentrations,respectively. Nearly no response was detected in 1.5 and 15 g/L OP exposed-fish. Data was curve-fittedevidencing a nonmonotonic dose?response curve. Interestingly, ER2 mRNA expression was augmentedabove baseline levels only when males were exposed to the lowest OP concentration. We speculate thatgenomic control of vitellogenesis is under control of multiple steroid receptors with different affinitiesfor ligands. ER isoform, only up-regulated with very low concentrations of ligand, would act as a sen-sors of OP (or E2) to induce ER and VTG. With high OP concentrations, the expression of ER isoform ispromptly augmented, with the concomitant VTG transactivation. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/32169 Lo Nostro, Fabiana Laura; Varela, María Luisa; Ferreira, Maria Florencia; Da Cuña, Rodrigo Hernán; Regueira, Mariana; Genovese, Griselda; et al.; Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae); Elsevier Science; Aquatic Toxicology; 156; 8-2014; 30-40 0166-445X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/32169 |
identifier_str_mv |
Lo Nostro, Fabiana Laura; Varela, María Luisa; Ferreira, Maria Florencia; Da Cuña, Rodrigo Hernán; Regueira, Mariana; Genovese, Griselda; et al.; Nonmonotonic response of vitellogenin and estrogen receptor geneexpression after octylphenol exposure of Cichlasoma dimerus(Perciformes, Cichlidae); Elsevier Science; Aquatic Toxicology; 156; 8-2014; 30-40 0166-445X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2014.07.019 info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0166445X14002550 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.13397 |