Biochemical and Morphological Alterations in Hearts of Copper-Deficient Bovines

Autores
Olivares, Roberto Walter Israel; Postma, Gabriela Cintia; Schapira, Andrea; Iglesias, Dario Ezequiel; Valdez, Laura Batriz; Breininger, Elizabeth; Gazzaneo, Pablo Daniel; Minatel, Leonardo
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Copper deficiency is an important disease of cattle that produces several clinical signs and lesions, due to alterations in copper-dependent enzymes. One of the organs affected by this deficiency is the heart (falling disease), but nevertheless, these cardiac lesions have not been extensively studied in bovines. The aim of this work was to propose a possible pathogenic mechanism for cardiac lesions in cattle affected by copper deficiency. Because of the possible existence of oxidative distress caused by low levels of copper-zinc-superoxide dismutase and cytochrome oxidase, ultrastructural and histological lesions have been evaluated in the heart of bovines in which a Cu deficiency had been induced using high molybdenum and sulfur levels in the diet. Our results indicated that copper deficiency produces significant damage in myocardium with high levels of lipid oxidation and a significant reduction in copper-zinc-superoxide dismutase activity leading to an oxidative distress situation. However, cytochrome oxidase activity was not significantly reduced. Histological observation revealed a significant increase in the amount of connective tissue, enlarged basement membranes of myocytes, and numerous Anichkov cells, in the hearts of deficient animals. Ultrastructural observation showed a significant enhancement in the mitochondrial volume density, with presence of lesions such as swelling and cristae disruption. We conclude that copper deficiency in bovines causes morphological lesions in the heart due to an oxidative damage produced by copper-dependent enzyme alterations.
Fil: Olivares, Roberto Walter Israel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Postma, Gabriela Cintia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Schapira, Andrea. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Iglesias, Dario Ezequiel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Valdez, Laura Batriz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Breininger, Elizabeth. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gazzaneo, Pablo Daniel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Minatel, Leonardo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Materia
CALVES
COPPER
HEART
OXIDATIVE DISTRESS
ULTRASTRUCTURE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/88355
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/88355
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biochemical and Morphological Alterations in Hearts of Copper-Deficient BovinesOlivares, Roberto Walter IsraelPostma, Gabriela CintiaSchapira, AndreaIglesias, Dario EzequielValdez, Laura BatrizBreininger, ElizabethGazzaneo, Pablo DanielMinatel, LeonardoCALVESCOPPERHEARTOXIDATIVE DISTRESSULTRASTRUCTUREhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Copper deficiency is an important disease of cattle that produces several clinical signs and lesions, due to alterations in copper-dependent enzymes. One of the organs affected by this deficiency is the heart (falling disease), but nevertheless, these cardiac lesions have not been extensively studied in bovines. The aim of this work was to propose a possible pathogenic mechanism for cardiac lesions in cattle affected by copper deficiency. Because of the possible existence of oxidative distress caused by low levels of copper-zinc-superoxide dismutase and cytochrome oxidase, ultrastructural and histological lesions have been evaluated in the heart of bovines in which a Cu deficiency had been induced using high molybdenum and sulfur levels in the diet. Our results indicated that copper deficiency produces significant damage in myocardium with high levels of lipid oxidation and a significant reduction in copper-zinc-superoxide dismutase activity leading to an oxidative distress situation. However, cytochrome oxidase activity was not significantly reduced. Histological observation revealed a significant increase in the amount of connective tissue, enlarged basement membranes of myocytes, and numerous Anichkov cells, in the hearts of deficient animals. Ultrastructural observation showed a significant enhancement in the mitochondrial volume density, with presence of lesions such as swelling and cristae disruption. We conclude that copper deficiency in bovines causes morphological lesions in the heart due to an oxidative damage produced by copper-dependent enzyme alterations.Fil: Olivares, Roberto Walter Israel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Postma, Gabriela Cintia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Schapira, Andrea. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Iglesias, Dario Ezequiel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Valdez, Laura Batriz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Breininger, Elizabeth. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gazzaneo, Pablo Daniel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Minatel, Leonardo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaHumana Press2018-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/88355Olivares, Roberto Walter Israel; Postma, Gabriela Cintia; Schapira, Andrea; Iglesias, Dario Ezequiel; Valdez, Laura Batriz; et al.; Biochemical and Morphological Alterations in Hearts of Copper-Deficient Bovines; Humana Press; Biological Trace Element Research; 189; 2; 8-2018; 447-4550163-4984CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s12011-018-1476-xinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs12011-018-1476-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:20:56Zoai:ri.conicet.gov.ar:11336/88355instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:20:56.886CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biochemical and Morphological Alterations in Hearts of Copper-Deficient Bovines
title Biochemical and Morphological Alterations in Hearts of Copper-Deficient Bovines
spellingShingle Biochemical and Morphological Alterations in Hearts of Copper-Deficient Bovines
Olivares, Roberto Walter Israel
CALVES
COPPER
HEART
OXIDATIVE DISTRESS
ULTRASTRUCTURE
title_short Biochemical and Morphological Alterations in Hearts of Copper-Deficient Bovines
title_full Biochemical and Morphological Alterations in Hearts of Copper-Deficient Bovines
title_fullStr Biochemical and Morphological Alterations in Hearts of Copper-Deficient Bovines
title_full_unstemmed Biochemical and Morphological Alterations in Hearts of Copper-Deficient Bovines
title_sort Biochemical and Morphological Alterations in Hearts of Copper-Deficient Bovines
dc.creator.none.fl_str_mv Olivares, Roberto Walter Israel
Postma, Gabriela Cintia
Schapira, Andrea
Iglesias, Dario Ezequiel
Valdez, Laura Batriz
Breininger, Elizabeth
Gazzaneo, Pablo Daniel
Minatel, Leonardo
author Olivares, Roberto Walter Israel
author_facet Olivares, Roberto Walter Israel
Postma, Gabriela Cintia
Schapira, Andrea
Iglesias, Dario Ezequiel
Valdez, Laura Batriz
Breininger, Elizabeth
Gazzaneo, Pablo Daniel
Minatel, Leonardo
author_role author
author2 Postma, Gabriela Cintia
Schapira, Andrea
Iglesias, Dario Ezequiel
Valdez, Laura Batriz
Breininger, Elizabeth
Gazzaneo, Pablo Daniel
Minatel, Leonardo
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CALVES
COPPER
HEART
OXIDATIVE DISTRESS
ULTRASTRUCTURE
topic CALVES
COPPER
HEART
OXIDATIVE DISTRESS
ULTRASTRUCTURE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Copper deficiency is an important disease of cattle that produces several clinical signs and lesions, due to alterations in copper-dependent enzymes. One of the organs affected by this deficiency is the heart (falling disease), but nevertheless, these cardiac lesions have not been extensively studied in bovines. The aim of this work was to propose a possible pathogenic mechanism for cardiac lesions in cattle affected by copper deficiency. Because of the possible existence of oxidative distress caused by low levels of copper-zinc-superoxide dismutase and cytochrome oxidase, ultrastructural and histological lesions have been evaluated in the heart of bovines in which a Cu deficiency had been induced using high molybdenum and sulfur levels in the diet. Our results indicated that copper deficiency produces significant damage in myocardium with high levels of lipid oxidation and a significant reduction in copper-zinc-superoxide dismutase activity leading to an oxidative distress situation. However, cytochrome oxidase activity was not significantly reduced. Histological observation revealed a significant increase in the amount of connective tissue, enlarged basement membranes of myocytes, and numerous Anichkov cells, in the hearts of deficient animals. Ultrastructural observation showed a significant enhancement in the mitochondrial volume density, with presence of lesions such as swelling and cristae disruption. We conclude that copper deficiency in bovines causes morphological lesions in the heart due to an oxidative damage produced by copper-dependent enzyme alterations.
Fil: Olivares, Roberto Walter Israel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Postma, Gabriela Cintia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Schapira, Andrea. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Iglesias, Dario Ezequiel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Valdez, Laura Batriz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Breininger, Elizabeth. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gazzaneo, Pablo Daniel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Minatel, Leonardo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
description Copper deficiency is an important disease of cattle that produces several clinical signs and lesions, due to alterations in copper-dependent enzymes. One of the organs affected by this deficiency is the heart (falling disease), but nevertheless, these cardiac lesions have not been extensively studied in bovines. The aim of this work was to propose a possible pathogenic mechanism for cardiac lesions in cattle affected by copper deficiency. Because of the possible existence of oxidative distress caused by low levels of copper-zinc-superoxide dismutase and cytochrome oxidase, ultrastructural and histological lesions have been evaluated in the heart of bovines in which a Cu deficiency had been induced using high molybdenum and sulfur levels in the diet. Our results indicated that copper deficiency produces significant damage in myocardium with high levels of lipid oxidation and a significant reduction in copper-zinc-superoxide dismutase activity leading to an oxidative distress situation. However, cytochrome oxidase activity was not significantly reduced. Histological observation revealed a significant increase in the amount of connective tissue, enlarged basement membranes of myocytes, and numerous Anichkov cells, in the hearts of deficient animals. Ultrastructural observation showed a significant enhancement in the mitochondrial volume density, with presence of lesions such as swelling and cristae disruption. We conclude that copper deficiency in bovines causes morphological lesions in the heart due to an oxidative damage produced by copper-dependent enzyme alterations.
publishDate 2018
dc.date.none.fl_str_mv 2018-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/88355
Olivares, Roberto Walter Israel; Postma, Gabriela Cintia; Schapira, Andrea; Iglesias, Dario Ezequiel; Valdez, Laura Batriz; et al.; Biochemical and Morphological Alterations in Hearts of Copper-Deficient Bovines; Humana Press; Biological Trace Element Research; 189; 2; 8-2018; 447-455
0163-4984
CONICET Digital
CONICET
url http://hdl.handle.net/11336/88355
identifier_str_mv Olivares, Roberto Walter Israel; Postma, Gabriela Cintia; Schapira, Andrea; Iglesias, Dario Ezequiel; Valdez, Laura Batriz; et al.; Biochemical and Morphological Alterations in Hearts of Copper-Deficient Bovines; Humana Press; Biological Trace Element Research; 189; 2; 8-2018; 447-455
0163-4984
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s12011-018-1476-x
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs12011-018-1476-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Humana Press
publisher.none.fl_str_mv Humana Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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