Exploring the effects of honey's anti-quorum sensing activity on bacterial cell membrane

Autores
Carnicero, Anabela; Robles, Alicia Daniela; Iurlina, Miriam Ofelia; Saiz, Amelia Ivone
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In recent years, honey is being assayed in the food industry to inhibit communication between pathogenic microorganisms. One strategy to attack antibiotic resistant strain is by the development of new ways that reduced the virulence expression. Here, we evaluated the anti-Quorum Sensing (QS) activity of multi-floral honeys using Chromobacterium violaceum. The disruption of the cell membrane was confirmed through FT-IR spectroscopy, hydrophobicity, and Scanning Electron Microscopy (SEM). Shifts in the frequency of the main peaks (cm 1), showed that bioactive compounds from honey affected the structure of the C. violaceum membrane. The most significant changes were observed at the membrane proteins and lipids level. The polarity of the cell membrane affects the diffusion of the inducing molecules. Supporting the changes in hydrophobicity, SEM revealed alterations in the appearance of the cell surface. Consequently, honey contributes to avoid bacterial injury by the disruption of membrane, and hence, cell communication and virulence.
Fil: Carnicero, Anabela. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Universidad Nacional de Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada; Argentina
Fil: Robles, Alicia Daniela. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Iurlina, Miriam Ofelia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Fil: Saiz, Amelia Ivone. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Materia
HONEY COMPOUNDS
CELL COMMUNICATION
FTIR
SEM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/263214
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Exploring the effects of honey's anti-quorum sensing activity on bacterial cell membraneCarnicero, AnabelaRobles, Alicia DanielaIurlina, Miriam OfeliaSaiz, Amelia IvoneHONEY COMPOUNDSCELL COMMUNICATIONFTIRSEMhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In recent years, honey is being assayed in the food industry to inhibit communication between pathogenic microorganisms. One strategy to attack antibiotic resistant strain is by the development of new ways that reduced the virulence expression. Here, we evaluated the anti-Quorum Sensing (QS) activity of multi-floral honeys using Chromobacterium violaceum. The disruption of the cell membrane was confirmed through FT-IR spectroscopy, hydrophobicity, and Scanning Electron Microscopy (SEM). Shifts in the frequency of the main peaks (cm 1), showed that bioactive compounds from honey affected the structure of the C. violaceum membrane. The most significant changes were observed at the membrane proteins and lipids level. The polarity of the cell membrane affects the diffusion of the inducing molecules. Supporting the changes in hydrophobicity, SEM revealed alterations in the appearance of the cell surface. Consequently, honey contributes to avoid bacterial injury by the disruption of membrane, and hence, cell communication and virulence.Fil: Carnicero, Anabela. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Universidad Nacional de Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada; ArgentinaFil: Robles, Alicia Daniela. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Iurlina, Miriam Ofelia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; ArgentinaFil: Saiz, Amelia Ivone. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; ArgentinaElsevier2024-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/263214Carnicero, Anabela; Robles, Alicia Daniela; Iurlina, Miriam Ofelia; Saiz, Amelia Ivone; Exploring the effects of honey's anti-quorum sensing activity on bacterial cell membrane; Elsevier; Food Chemistry Advances; 4; 100701; 6-2024; 1-82772-753XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2772753X24000972info:eu-repo/semantics/altIdentifier/doi/10.1016/j.focha.2024.100701info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:00Zoai:ri.conicet.gov.ar:11336/263214instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:00.738CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Exploring the effects of honey's anti-quorum sensing activity on bacterial cell membrane
title Exploring the effects of honey's anti-quorum sensing activity on bacterial cell membrane
spellingShingle Exploring the effects of honey's anti-quorum sensing activity on bacterial cell membrane
Carnicero, Anabela
HONEY COMPOUNDS
CELL COMMUNICATION
FTIR
SEM
title_short Exploring the effects of honey's anti-quorum sensing activity on bacterial cell membrane
title_full Exploring the effects of honey's anti-quorum sensing activity on bacterial cell membrane
title_fullStr Exploring the effects of honey's anti-quorum sensing activity on bacterial cell membrane
title_full_unstemmed Exploring the effects of honey's anti-quorum sensing activity on bacterial cell membrane
title_sort Exploring the effects of honey's anti-quorum sensing activity on bacterial cell membrane
dc.creator.none.fl_str_mv Carnicero, Anabela
Robles, Alicia Daniela
Iurlina, Miriam Ofelia
Saiz, Amelia Ivone
author Carnicero, Anabela
author_facet Carnicero, Anabela
Robles, Alicia Daniela
Iurlina, Miriam Ofelia
Saiz, Amelia Ivone
author_role author
author2 Robles, Alicia Daniela
Iurlina, Miriam Ofelia
Saiz, Amelia Ivone
author2_role author
author
author
dc.subject.none.fl_str_mv HONEY COMPOUNDS
CELL COMMUNICATION
FTIR
SEM
topic HONEY COMPOUNDS
CELL COMMUNICATION
FTIR
SEM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In recent years, honey is being assayed in the food industry to inhibit communication between pathogenic microorganisms. One strategy to attack antibiotic resistant strain is by the development of new ways that reduced the virulence expression. Here, we evaluated the anti-Quorum Sensing (QS) activity of multi-floral honeys using Chromobacterium violaceum. The disruption of the cell membrane was confirmed through FT-IR spectroscopy, hydrophobicity, and Scanning Electron Microscopy (SEM). Shifts in the frequency of the main peaks (cm 1), showed that bioactive compounds from honey affected the structure of the C. violaceum membrane. The most significant changes were observed at the membrane proteins and lipids level. The polarity of the cell membrane affects the diffusion of the inducing molecules. Supporting the changes in hydrophobicity, SEM revealed alterations in the appearance of the cell surface. Consequently, honey contributes to avoid bacterial injury by the disruption of membrane, and hence, cell communication and virulence.
Fil: Carnicero, Anabela. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Universidad Nacional de Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada; Argentina
Fil: Robles, Alicia Daniela. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Iurlina, Miriam Ofelia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Fil: Saiz, Amelia Ivone. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
description In recent years, honey is being assayed in the food industry to inhibit communication between pathogenic microorganisms. One strategy to attack antibiotic resistant strain is by the development of new ways that reduced the virulence expression. Here, we evaluated the anti-Quorum Sensing (QS) activity of multi-floral honeys using Chromobacterium violaceum. The disruption of the cell membrane was confirmed through FT-IR spectroscopy, hydrophobicity, and Scanning Electron Microscopy (SEM). Shifts in the frequency of the main peaks (cm 1), showed that bioactive compounds from honey affected the structure of the C. violaceum membrane. The most significant changes were observed at the membrane proteins and lipids level. The polarity of the cell membrane affects the diffusion of the inducing molecules. Supporting the changes in hydrophobicity, SEM revealed alterations in the appearance of the cell surface. Consequently, honey contributes to avoid bacterial injury by the disruption of membrane, and hence, cell communication and virulence.
publishDate 2024
dc.date.none.fl_str_mv 2024-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/263214
Carnicero, Anabela; Robles, Alicia Daniela; Iurlina, Miriam Ofelia; Saiz, Amelia Ivone; Exploring the effects of honey's anti-quorum sensing activity on bacterial cell membrane; Elsevier; Food Chemistry Advances; 4; 100701; 6-2024; 1-8
2772-753X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/263214
identifier_str_mv Carnicero, Anabela; Robles, Alicia Daniela; Iurlina, Miriam Ofelia; Saiz, Amelia Ivone; Exploring the effects of honey's anti-quorum sensing activity on bacterial cell membrane; Elsevier; Food Chemistry Advances; 4; 100701; 6-2024; 1-8
2772-753X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2772753X24000972
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.focha.2024.100701
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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