Development of a real-time PCR assay for the detection of Trichinella Spiralis in muscle tissue of swine and derivatives

Autores
Quintana, Silvina; Recavarren, Mariana Ines; Scialfa, Exequiel Alejandro; Viera I.; Rivero, Mariana Alejandra; Krivokapich, Silvio Jesús
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trichinellosis is an emergent zoonosis in several regions of the world and it is considered a public health problem. Trichinellosis represent one of the most important zoonotic diseases in Argentina. The purpose of this study was to develop a real-time PCR assay with internal control to detect Trichinella spiralis DNA in samples of swine muscles and its derivatives. PCR amplification of DNA from muscle samples was performed by real-time PCR with an internal porcine DNA amplification control. The developed PCR assay specifically detects T. spiralis showing no amplification with other Trichinella genotypes and showed an estimated sensitivity of 0.024 larvae per gram in swine muscle samples. From the 21 samples analyzed, five samples negative by enzymatic digestion were positive by real-time PCR, which demonstrates that this technique is capable of detecting T. spiralis in cases when enzymatic digestion cannot. In this work, a new molecular assay with internal control for T. spiralis detection was successfully developed.
Fil: Quintana, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Fares Taie Biochemical Analysis Institute; Argentina
Fil: Recavarren, Mariana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Fares Taie Biochemical Analysis Institute; Argentina
Fil: Scialfa, Exequiel Alejandro. Rural Zoonosis Division Azul; Argentina
Fil: Viera I.. Fares Taie Biochemical Analysis Institute; Argentina
Fil: Rivero, Mariana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Krivokapich, Silvio Jesús. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina
Materia
TRICHINELLA SPIRALIS
REAL TIME PCR
DISGNOSTICO
PORCINOS Y DERIVADOS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/180415
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/180415
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of a real-time PCR assay for the detection of Trichinella Spiralis in muscle tissue of swine and derivativesQuintana, SilvinaRecavarren, Mariana InesScialfa, Exequiel AlejandroViera I.Rivero, Mariana AlejandraKrivokapich, Silvio JesúsTRICHINELLA SPIRALISREAL TIME PCRDISGNOSTICOPORCINOS Y DERIVADOShttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Trichinellosis is an emergent zoonosis in several regions of the world and it is considered a public health problem. Trichinellosis represent one of the most important zoonotic diseases in Argentina. The purpose of this study was to develop a real-time PCR assay with internal control to detect Trichinella spiralis DNA in samples of swine muscles and its derivatives. PCR amplification of DNA from muscle samples was performed by real-time PCR with an internal porcine DNA amplification control. The developed PCR assay specifically detects T. spiralis showing no amplification with other Trichinella genotypes and showed an estimated sensitivity of 0.024 larvae per gram in swine muscle samples. From the 21 samples analyzed, five samples negative by enzymatic digestion were positive by real-time PCR, which demonstrates that this technique is capable of detecting T. spiralis in cases when enzymatic digestion cannot. In this work, a new molecular assay with internal control for T. spiralis detection was successfully developed.Fil: Quintana, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Fares Taie Biochemical Analysis Institute; ArgentinaFil: Recavarren, Mariana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Fares Taie Biochemical Analysis Institute; ArgentinaFil: Scialfa, Exequiel Alejandro. Rural Zoonosis Division Azul; ArgentinaFil: Viera I.. Fares Taie Biochemical Analysis Institute; ArgentinaFil: Rivero, Mariana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Krivokapich, Silvio Jesús. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; ArgentinaWiley Blackwell Publishing, Inc2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/180415Quintana, Silvina; Recavarren, Mariana Ines; Scialfa, Exequiel Alejandro; Viera I.; Rivero, Mariana Alejandra; et al.; Development of a real-time PCR assay for the detection of Trichinella Spiralis in muscle tissue of swine and derivatives; Wiley Blackwell Publishing, Inc; Journal of Food Safety; 36; 2; 12-2015; 282-2870149-6085CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/jfs.12244info:eu-repo/semantics/altIdentifier/doi/ 10.1111/jfs.12244info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:02:11Zoai:ri.conicet.gov.ar:11336/180415instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:02:11.42CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of a real-time PCR assay for the detection of Trichinella Spiralis in muscle tissue of swine and derivatives
title Development of a real-time PCR assay for the detection of Trichinella Spiralis in muscle tissue of swine and derivatives
spellingShingle Development of a real-time PCR assay for the detection of Trichinella Spiralis in muscle tissue of swine and derivatives
Quintana, Silvina
TRICHINELLA SPIRALIS
REAL TIME PCR
DISGNOSTICO
PORCINOS Y DERIVADOS
title_short Development of a real-time PCR assay for the detection of Trichinella Spiralis in muscle tissue of swine and derivatives
title_full Development of a real-time PCR assay for the detection of Trichinella Spiralis in muscle tissue of swine and derivatives
title_fullStr Development of a real-time PCR assay for the detection of Trichinella Spiralis in muscle tissue of swine and derivatives
title_full_unstemmed Development of a real-time PCR assay for the detection of Trichinella Spiralis in muscle tissue of swine and derivatives
title_sort Development of a real-time PCR assay for the detection of Trichinella Spiralis in muscle tissue of swine and derivatives
dc.creator.none.fl_str_mv Quintana, Silvina
Recavarren, Mariana Ines
Scialfa, Exequiel Alejandro
Viera I.
Rivero, Mariana Alejandra
Krivokapich, Silvio Jesús
author Quintana, Silvina
author_facet Quintana, Silvina
Recavarren, Mariana Ines
Scialfa, Exequiel Alejandro
Viera I.
Rivero, Mariana Alejandra
Krivokapich, Silvio Jesús
author_role author
author2 Recavarren, Mariana Ines
Scialfa, Exequiel Alejandro
Viera I.
Rivero, Mariana Alejandra
Krivokapich, Silvio Jesús
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv TRICHINELLA SPIRALIS
REAL TIME PCR
DISGNOSTICO
PORCINOS Y DERIVADOS
topic TRICHINELLA SPIRALIS
REAL TIME PCR
DISGNOSTICO
PORCINOS Y DERIVADOS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Trichinellosis is an emergent zoonosis in several regions of the world and it is considered a public health problem. Trichinellosis represent one of the most important zoonotic diseases in Argentina. The purpose of this study was to develop a real-time PCR assay with internal control to detect Trichinella spiralis DNA in samples of swine muscles and its derivatives. PCR amplification of DNA from muscle samples was performed by real-time PCR with an internal porcine DNA amplification control. The developed PCR assay specifically detects T. spiralis showing no amplification with other Trichinella genotypes and showed an estimated sensitivity of 0.024 larvae per gram in swine muscle samples. From the 21 samples analyzed, five samples negative by enzymatic digestion were positive by real-time PCR, which demonstrates that this technique is capable of detecting T. spiralis in cases when enzymatic digestion cannot. In this work, a new molecular assay with internal control for T. spiralis detection was successfully developed.
Fil: Quintana, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Fares Taie Biochemical Analysis Institute; Argentina
Fil: Recavarren, Mariana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Fares Taie Biochemical Analysis Institute; Argentina
Fil: Scialfa, Exequiel Alejandro. Rural Zoonosis Division Azul; Argentina
Fil: Viera I.. Fares Taie Biochemical Analysis Institute; Argentina
Fil: Rivero, Mariana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Krivokapich, Silvio Jesús. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina
description Trichinellosis is an emergent zoonosis in several regions of the world and it is considered a public health problem. Trichinellosis represent one of the most important zoonotic diseases in Argentina. The purpose of this study was to develop a real-time PCR assay with internal control to detect Trichinella spiralis DNA in samples of swine muscles and its derivatives. PCR amplification of DNA from muscle samples was performed by real-time PCR with an internal porcine DNA amplification control. The developed PCR assay specifically detects T. spiralis showing no amplification with other Trichinella genotypes and showed an estimated sensitivity of 0.024 larvae per gram in swine muscle samples. From the 21 samples analyzed, five samples negative by enzymatic digestion were positive by real-time PCR, which demonstrates that this technique is capable of detecting T. spiralis in cases when enzymatic digestion cannot. In this work, a new molecular assay with internal control for T. spiralis detection was successfully developed.
publishDate 2015
dc.date.none.fl_str_mv 2015-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/180415
Quintana, Silvina; Recavarren, Mariana Ines; Scialfa, Exequiel Alejandro; Viera I.; Rivero, Mariana Alejandra; et al.; Development of a real-time PCR assay for the detection of Trichinella Spiralis in muscle tissue of swine and derivatives; Wiley Blackwell Publishing, Inc; Journal of Food Safety; 36; 2; 12-2015; 282-287
0149-6085
CONICET Digital
CONICET
url http://hdl.handle.net/11336/180415
identifier_str_mv Quintana, Silvina; Recavarren, Mariana Ines; Scialfa, Exequiel Alejandro; Viera I.; Rivero, Mariana Alejandra; et al.; Development of a real-time PCR assay for the detection of Trichinella Spiralis in muscle tissue of swine and derivatives; Wiley Blackwell Publishing, Inc; Journal of Food Safety; 36; 2; 12-2015; 282-287
0149-6085
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/jfs.12244
info:eu-repo/semantics/altIdentifier/doi/ 10.1111/jfs.12244
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980000208257024
score 12.993085

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