Synthesis, physicochemical characterization and biological activity of anandamide/ε-polycaprolactone nanoparticles obtained by electrospraying

Autores
Martín Giménez, Virna Margarita; Russo, Marcos Guillermo; Narda, Griselda Edith; Fuentes, Lucía B.; Mazzei, Luciana Jorgelina; Gamarra Luques, Carlos Diego; Kassuha, Diego Enrique; Manucha, Walter Ariel Fernando
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Drug encapsulation in nanocarriers such as polymeric nanoparticles (Nps) may help to overcome the limitations associated with cannabinoids. In this sense, our work aimed to highlight the use of electrospraying technique for the development of carrier Nps of anandamide (AEA), an endocannabinoid with attractive pharmacological effects but underestimated due to its unfavorable physicochemical and pharmacokinetic properties added to its undesirable effects at the level of the central nervous system. We characterized physicochemically and evaluated in vitro biological activity of anandamide/ε-polycaprolactone nanoparticles (Nps-AEA/PCL) obtained by electrospraying in epithelial cells of human proximal tubule (HK2), to prove the utility of this method and to validate the biological effect of Nps-AEA/PCL. We obtained particles from 100 to 900 nm of diameter with a predominance of 200 a 400 nm. Their zeta potential was -20±1.86 mV. We demonstrated the stable encapsulation of AEA in Nps-AEA/PCL, as well as its dose-dependent capacity to induce the expression of iNOS and NO levels and to decrease the Na+/K+ ATPase activity in HK2 cells. Obtaining Nps-AEA/PCL by electrospraying would represent a promising methodology for a novel AEA pharmaceutical formulation development with optimal physicochemical properties, physical stability and biological activity on HK2 cells.
Fil: Martín Giménez, Virna Margarita. Universidad Católica de Cuyo - Sede San Juan; Argentina
Fil: Russo, Marcos Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Narda, Griselda Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Fuentes, Lucía B.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Mazzei, Luciana Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Gamarra Luques, Carlos Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Kassuha, Diego Enrique. Universidad Católica de Cuyo - Sede San Juan; Argentina
Fil: Manucha, Walter Ariel Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Materia
NANOBIOTECHNOLOGY
NANO-DRUG
ANANDAMIDE
HYPERTENSION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/109060
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Synthesis, physicochemical characterization and biological activity of anandamide/ε-polycaprolactone nanoparticles obtained by electrosprayingMartín Giménez, Virna MargaritaRusso, Marcos GuillermoNarda, Griselda EdithFuentes, Lucía B.Mazzei, Luciana JorgelinaGamarra Luques, Carlos DiegoKassuha, Diego EnriqueManucha, Walter Ariel FernandoNANOBIOTECHNOLOGYNANO-DRUGANANDAMIDEHYPERTENSIONhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Drug encapsulation in nanocarriers such as polymeric nanoparticles (Nps) may help to overcome the limitations associated with cannabinoids. In this sense, our work aimed to highlight the use of electrospraying technique for the development of carrier Nps of anandamide (AEA), an endocannabinoid with attractive pharmacological effects but underestimated due to its unfavorable physicochemical and pharmacokinetic properties added to its undesirable effects at the level of the central nervous system. We characterized physicochemically and evaluated in vitro biological activity of anandamide/ε-polycaprolactone nanoparticles (Nps-AEA/PCL) obtained by electrospraying in epithelial cells of human proximal tubule (HK2), to prove the utility of this method and to validate the biological effect of Nps-AEA/PCL. We obtained particles from 100 to 900 nm of diameter with a predominance of 200 a 400 nm. Their zeta potential was -20±1.86 mV. We demonstrated the stable encapsulation of AEA in Nps-AEA/PCL, as well as its dose-dependent capacity to induce the expression of iNOS and NO levels and to decrease the Na+/K+ ATPase activity in HK2 cells. Obtaining Nps-AEA/PCL by electrospraying would represent a promising methodology for a novel AEA pharmaceutical formulation development with optimal physicochemical properties, physical stability and biological activity on HK2 cells.Fil: Martín Giménez, Virna Margarita. Universidad Católica de Cuyo - Sede San Juan; ArgentinaFil: Russo, Marcos Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; ArgentinaFil: Narda, Griselda Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; ArgentinaFil: Fuentes, Lucía B.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Mazzei, Luciana Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Gamarra Luques, Carlos Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Kassuha, Diego Enrique. Universidad Católica de Cuyo - Sede San Juan; ArgentinaFil: Manucha, Walter Ariel Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaInstitution of Engineering and Technology2019-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/109060Martín Giménez, Virna Margarita; Russo, Marcos Guillermo; Narda, Griselda Edith; Fuentes, Lucía B.; Mazzei, Luciana Jorgelina; et al.; Synthesis, physicochemical characterization and biological activity of anandamide/ε-polycaprolactone nanoparticles obtained by electrospraying; Institution of Engineering and Technology; Iet Nanobiotechnology; 14; 1; 11-2019; 86-931751-8741CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://digital-library.theiet.org/content/journals/10.1049/iet-nbt.2019.0108info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:39:46Zoai:ri.conicet.gov.ar:11336/109060instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:46.443CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Synthesis, physicochemical characterization and biological activity of anandamide/ε-polycaprolactone nanoparticles obtained by electrospraying
title Synthesis, physicochemical characterization and biological activity of anandamide/ε-polycaprolactone nanoparticles obtained by electrospraying
spellingShingle Synthesis, physicochemical characterization and biological activity of anandamide/ε-polycaprolactone nanoparticles obtained by electrospraying
Martín Giménez, Virna Margarita
NANOBIOTECHNOLOGY
NANO-DRUG
ANANDAMIDE
HYPERTENSION
title_short Synthesis, physicochemical characterization and biological activity of anandamide/ε-polycaprolactone nanoparticles obtained by electrospraying
title_full Synthesis, physicochemical characterization and biological activity of anandamide/ε-polycaprolactone nanoparticles obtained by electrospraying
title_fullStr Synthesis, physicochemical characterization and biological activity of anandamide/ε-polycaprolactone nanoparticles obtained by electrospraying
title_full_unstemmed Synthesis, physicochemical characterization and biological activity of anandamide/ε-polycaprolactone nanoparticles obtained by electrospraying
title_sort Synthesis, physicochemical characterization and biological activity of anandamide/ε-polycaprolactone nanoparticles obtained by electrospraying
dc.creator.none.fl_str_mv Martín Giménez, Virna Margarita
Russo, Marcos Guillermo
Narda, Griselda Edith
Fuentes, Lucía B.
Mazzei, Luciana Jorgelina
Gamarra Luques, Carlos Diego
Kassuha, Diego Enrique
Manucha, Walter Ariel Fernando
author Martín Giménez, Virna Margarita
author_facet Martín Giménez, Virna Margarita
Russo, Marcos Guillermo
Narda, Griselda Edith
Fuentes, Lucía B.
Mazzei, Luciana Jorgelina
Gamarra Luques, Carlos Diego
Kassuha, Diego Enrique
Manucha, Walter Ariel Fernando
author_role author
author2 Russo, Marcos Guillermo
Narda, Griselda Edith
Fuentes, Lucía B.
Mazzei, Luciana Jorgelina
Gamarra Luques, Carlos Diego
Kassuha, Diego Enrique
Manucha, Walter Ariel Fernando
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv NANOBIOTECHNOLOGY
NANO-DRUG
ANANDAMIDE
HYPERTENSION
topic NANOBIOTECHNOLOGY
NANO-DRUG
ANANDAMIDE
HYPERTENSION
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Drug encapsulation in nanocarriers such as polymeric nanoparticles (Nps) may help to overcome the limitations associated with cannabinoids. In this sense, our work aimed to highlight the use of electrospraying technique for the development of carrier Nps of anandamide (AEA), an endocannabinoid with attractive pharmacological effects but underestimated due to its unfavorable physicochemical and pharmacokinetic properties added to its undesirable effects at the level of the central nervous system. We characterized physicochemically and evaluated in vitro biological activity of anandamide/ε-polycaprolactone nanoparticles (Nps-AEA/PCL) obtained by electrospraying in epithelial cells of human proximal tubule (HK2), to prove the utility of this method and to validate the biological effect of Nps-AEA/PCL. We obtained particles from 100 to 900 nm of diameter with a predominance of 200 a 400 nm. Their zeta potential was -20±1.86 mV. We demonstrated the stable encapsulation of AEA in Nps-AEA/PCL, as well as its dose-dependent capacity to induce the expression of iNOS and NO levels and to decrease the Na+/K+ ATPase activity in HK2 cells. Obtaining Nps-AEA/PCL by electrospraying would represent a promising methodology for a novel AEA pharmaceutical formulation development with optimal physicochemical properties, physical stability and biological activity on HK2 cells.
Fil: Martín Giménez, Virna Margarita. Universidad Católica de Cuyo - Sede San Juan; Argentina
Fil: Russo, Marcos Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Narda, Griselda Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Fuentes, Lucía B.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Mazzei, Luciana Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Gamarra Luques, Carlos Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Kassuha, Diego Enrique. Universidad Católica de Cuyo - Sede San Juan; Argentina
Fil: Manucha, Walter Ariel Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
description Drug encapsulation in nanocarriers such as polymeric nanoparticles (Nps) may help to overcome the limitations associated with cannabinoids. In this sense, our work aimed to highlight the use of electrospraying technique for the development of carrier Nps of anandamide (AEA), an endocannabinoid with attractive pharmacological effects but underestimated due to its unfavorable physicochemical and pharmacokinetic properties added to its undesirable effects at the level of the central nervous system. We characterized physicochemically and evaluated in vitro biological activity of anandamide/ε-polycaprolactone nanoparticles (Nps-AEA/PCL) obtained by electrospraying in epithelial cells of human proximal tubule (HK2), to prove the utility of this method and to validate the biological effect of Nps-AEA/PCL. We obtained particles from 100 to 900 nm of diameter with a predominance of 200 a 400 nm. Their zeta potential was -20±1.86 mV. We demonstrated the stable encapsulation of AEA in Nps-AEA/PCL, as well as its dose-dependent capacity to induce the expression of iNOS and NO levels and to decrease the Na+/K+ ATPase activity in HK2 cells. Obtaining Nps-AEA/PCL by electrospraying would represent a promising methodology for a novel AEA pharmaceutical formulation development with optimal physicochemical properties, physical stability and biological activity on HK2 cells.
publishDate 2019
dc.date.none.fl_str_mv 2019-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/109060
Martín Giménez, Virna Margarita; Russo, Marcos Guillermo; Narda, Griselda Edith; Fuentes, Lucía B.; Mazzei, Luciana Jorgelina; et al.; Synthesis, physicochemical characterization and biological activity of anandamide/ε-polycaprolactone nanoparticles obtained by electrospraying; Institution of Engineering and Technology; Iet Nanobiotechnology; 14; 1; 11-2019; 86-93
1751-8741
CONICET Digital
CONICET
url http://hdl.handle.net/11336/109060
identifier_str_mv Martín Giménez, Virna Margarita; Russo, Marcos Guillermo; Narda, Griselda Edith; Fuentes, Lucía B.; Mazzei, Luciana Jorgelina; et al.; Synthesis, physicochemical characterization and biological activity of anandamide/ε-polycaprolactone nanoparticles obtained by electrospraying; Institution of Engineering and Technology; Iet Nanobiotechnology; 14; 1; 11-2019; 86-93
1751-8741
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://digital-library.theiet.org/content/journals/10.1049/iet-nbt.2019.0108
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Institution of Engineering and Technology
publisher.none.fl_str_mv Institution of Engineering and Technology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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