In vivo antileishmanial efficacy of miltefosine against Leishmania (Leishmania) amazonensis

Autores
Garcia Bustos, Maria Fernanda; Barrio, Alejandra; Prieto, Gabriela G.; De Raspi, Emma M.; Cimino, Rubén Oscar; Cardozo, Rubén Marino; Parada, Luis Antonio; Yeo, Matthew; Soto, Jaime; Uncos, Delfor Alejandro; Parodi Ramoneda, Cecilia María; Basombrío, Miguel Ángel Manuel
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Leishmaniasis, a disease caused by parasites of the Leishmania genus, constitutes a significant health and social problem in many countries and is increasing worldwide. The conventional treatment, meglumine antimoniate (MA), presents numerous disadvantages, including invasiveness, toxicity, and frequent therapeutic failure, justifying the attempts at finding alternatives to the first-line therapy. We have studied the comparative long-term efficacy of MA against miltefosine (MF) in Leishmania infection in experimental mice. The criteria for efficacy evaluation were footpad lesion size, anti-Leishmania antibodies level, histopathology of the site of inoculation (right footpad, RFP), splenic index (SI), and the presence of parasites in RFP, spleen, and liver, determined by polymerase chain reaction (PCR). Swiss mice, infected with Leishmania (Leishmania) amazonensis were treated, at different time points (5 and 40 days after infection) with either MA or MF. The efficacy of MF was better than that of MA for inhibiting lesions and for reducing tissue damage and presence/load of amastigotes in spleen and liver. Moreover, early administration of MF produced a clear reduction in splenomegaly and was equal in reducing antibody titles in comparison with MA. Our results demonstrated that MF is an effective and safe therapeutic alternative for leishmaniasis by L. (L.) amazonensis and is more efficacious than MA.
Fil: Garcia Bustos, Maria Fernanda. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barrio, Alejandra. Universidad Nacional de Salta; Argentina
Fil: Prieto, Gabriela G.. Universidad Nacional de Salta; Argentina
Fil: De Raspi, Emma M.. Universidad Nacional de Salta; Argentina
Fil: Cimino, Rubén Oscar. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cardozo, Rubén Marino. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Parada, Luis Antonio. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Yeo, Matthew. London School of Hygiene and Tropical Medicine; Reino Unido
Fil: Soto, Jaime. Universidad Nacional de Salta; Argentina. Fundación FADER; Colombia
Fil: Uncos, Delfor Alejandro. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Parodi Ramoneda, Cecilia María. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Basombrío, Miguel Ángel Manuel. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Miltefosine
Leishmania
Amazonensis
Efficacy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37136
Acceder
id CONICETDig_7c0e3f6cc33fc3d75994aeda1f5f0950
oai_identifier_str oai:ri.conicet.gov.ar:11336/37136
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vivo antileishmanial efficacy of miltefosine against Leishmania (Leishmania) amazonensisGarcia Bustos, Maria FernandaBarrio, AlejandraPrieto, Gabriela G.De Raspi, Emma M.Cimino, Rubén OscarCardozo, Rubén MarinoParada, Luis AntonioYeo, MatthewSoto, JaimeUncos, Delfor AlejandroParodi Ramoneda, Cecilia MaríaBasombrío, Miguel Ángel ManuelMiltefosineLeishmaniaAmazonensisEfficacyhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Leishmaniasis, a disease caused by parasites of the Leishmania genus, constitutes a significant health and social problem in many countries and is increasing worldwide. The conventional treatment, meglumine antimoniate (MA), presents numerous disadvantages, including invasiveness, toxicity, and frequent therapeutic failure, justifying the attempts at finding alternatives to the first-line therapy. We have studied the comparative long-term efficacy of MA against miltefosine (MF) in Leishmania infection in experimental mice. The criteria for efficacy evaluation were footpad lesion size, anti-Leishmania antibodies level, histopathology of the site of inoculation (right footpad, RFP), splenic index (SI), and the presence of parasites in RFP, spleen, and liver, determined by polymerase chain reaction (PCR). Swiss mice, infected with Leishmania (Leishmania) amazonensis were treated, at different time points (5 and 40 days after infection) with either MA or MF. The efficacy of MF was better than that of MA for inhibiting lesions and for reducing tissue damage and presence/load of amastigotes in spleen and liver. Moreover, early administration of MF produced a clear reduction in splenomegaly and was equal in reducing antibody titles in comparison with MA. Our results demonstrated that MF is an effective and safe therapeutic alternative for leishmaniasis by L. (L.) amazonensis and is more efficacious than MA.Fil: Garcia Bustos, Maria Fernanda. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barrio, Alejandra. Universidad Nacional de Salta; ArgentinaFil: Prieto, Gabriela G.. Universidad Nacional de Salta; ArgentinaFil: De Raspi, Emma M.. Universidad Nacional de Salta; ArgentinaFil: Cimino, Rubén Oscar. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cardozo, Rubén Marino. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Parada, Luis Antonio. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Yeo, Matthew. London School of Hygiene and Tropical Medicine; Reino UnidoFil: Soto, Jaime. Universidad Nacional de Salta; Argentina. Fundación FADER; ColombiaFil: Uncos, Delfor Alejandro. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Parodi Ramoneda, Cecilia María. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Basombrío, Miguel Ángel Manuel. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaAmerican Society of Parasitologists2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37136Garcia Bustos, Maria Fernanda; Barrio, Alejandra; Prieto, Gabriela G.; De Raspi, Emma M.; Cimino, Rubén Oscar; et al.; In vivo antileishmanial efficacy of miltefosine against Leishmania (Leishmania) amazonensis; American Society of Parasitologists; Journal of Parasitology; 100; 6; 12-2014; 840-8470022-3395CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1645/13-376.1info:eu-repo/semantics/altIdentifier/url/http://www.bioone.org/doi/abs/10.1645/13-376.1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:50Zoai:ri.conicet.gov.ar:11336/37136instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:50.4CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vivo antileishmanial efficacy of miltefosine against Leishmania (Leishmania) amazonensis
title In vivo antileishmanial efficacy of miltefosine against Leishmania (Leishmania) amazonensis
spellingShingle In vivo antileishmanial efficacy of miltefosine against Leishmania (Leishmania) amazonensis
Garcia Bustos, Maria Fernanda
Miltefosine
Leishmania
Amazonensis
Efficacy
title_short In vivo antileishmanial efficacy of miltefosine against Leishmania (Leishmania) amazonensis
title_full In vivo antileishmanial efficacy of miltefosine against Leishmania (Leishmania) amazonensis
title_fullStr In vivo antileishmanial efficacy of miltefosine against Leishmania (Leishmania) amazonensis
title_full_unstemmed In vivo antileishmanial efficacy of miltefosine against Leishmania (Leishmania) amazonensis
title_sort In vivo antileishmanial efficacy of miltefosine against Leishmania (Leishmania) amazonensis
dc.creator.none.fl_str_mv Garcia Bustos, Maria Fernanda
Barrio, Alejandra
Prieto, Gabriela G.
De Raspi, Emma M.
Cimino, Rubén Oscar
Cardozo, Rubén Marino
Parada, Luis Antonio
Yeo, Matthew
Soto, Jaime
Uncos, Delfor Alejandro
Parodi Ramoneda, Cecilia María
Basombrío, Miguel Ángel Manuel
author Garcia Bustos, Maria Fernanda
author_facet Garcia Bustos, Maria Fernanda
Barrio, Alejandra
Prieto, Gabriela G.
De Raspi, Emma M.
Cimino, Rubén Oscar
Cardozo, Rubén Marino
Parada, Luis Antonio
Yeo, Matthew
Soto, Jaime
Uncos, Delfor Alejandro
Parodi Ramoneda, Cecilia María
Basombrío, Miguel Ángel Manuel
author_role author
author2 Barrio, Alejandra
Prieto, Gabriela G.
De Raspi, Emma M.
Cimino, Rubén Oscar
Cardozo, Rubén Marino
Parada, Luis Antonio
Yeo, Matthew
Soto, Jaime
Uncos, Delfor Alejandro
Parodi Ramoneda, Cecilia María
Basombrío, Miguel Ángel Manuel
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Miltefosine
Leishmania
Amazonensis
Efficacy
topic Miltefosine
Leishmania
Amazonensis
Efficacy
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Leishmaniasis, a disease caused by parasites of the Leishmania genus, constitutes a significant health and social problem in many countries and is increasing worldwide. The conventional treatment, meglumine antimoniate (MA), presents numerous disadvantages, including invasiveness, toxicity, and frequent therapeutic failure, justifying the attempts at finding alternatives to the first-line therapy. We have studied the comparative long-term efficacy of MA against miltefosine (MF) in Leishmania infection in experimental mice. The criteria for efficacy evaluation were footpad lesion size, anti-Leishmania antibodies level, histopathology of the site of inoculation (right footpad, RFP), splenic index (SI), and the presence of parasites in RFP, spleen, and liver, determined by polymerase chain reaction (PCR). Swiss mice, infected with Leishmania (Leishmania) amazonensis were treated, at different time points (5 and 40 days after infection) with either MA or MF. The efficacy of MF was better than that of MA for inhibiting lesions and for reducing tissue damage and presence/load of amastigotes in spleen and liver. Moreover, early administration of MF produced a clear reduction in splenomegaly and was equal in reducing antibody titles in comparison with MA. Our results demonstrated that MF is an effective and safe therapeutic alternative for leishmaniasis by L. (L.) amazonensis and is more efficacious than MA.
Fil: Garcia Bustos, Maria Fernanda. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barrio, Alejandra. Universidad Nacional de Salta; Argentina
Fil: Prieto, Gabriela G.. Universidad Nacional de Salta; Argentina
Fil: De Raspi, Emma M.. Universidad Nacional de Salta; Argentina
Fil: Cimino, Rubén Oscar. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cardozo, Rubén Marino. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Parada, Luis Antonio. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Yeo, Matthew. London School of Hygiene and Tropical Medicine; Reino Unido
Fil: Soto, Jaime. Universidad Nacional de Salta; Argentina. Fundación FADER; Colombia
Fil: Uncos, Delfor Alejandro. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Parodi Ramoneda, Cecilia María. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Basombrío, Miguel Ángel Manuel. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Leishmaniasis, a disease caused by parasites of the Leishmania genus, constitutes a significant health and social problem in many countries and is increasing worldwide. The conventional treatment, meglumine antimoniate (MA), presents numerous disadvantages, including invasiveness, toxicity, and frequent therapeutic failure, justifying the attempts at finding alternatives to the first-line therapy. We have studied the comparative long-term efficacy of MA against miltefosine (MF) in Leishmania infection in experimental mice. The criteria for efficacy evaluation were footpad lesion size, anti-Leishmania antibodies level, histopathology of the site of inoculation (right footpad, RFP), splenic index (SI), and the presence of parasites in RFP, spleen, and liver, determined by polymerase chain reaction (PCR). Swiss mice, infected with Leishmania (Leishmania) amazonensis were treated, at different time points (5 and 40 days after infection) with either MA or MF. The efficacy of MF was better than that of MA for inhibiting lesions and for reducing tissue damage and presence/load of amastigotes in spleen and liver. Moreover, early administration of MF produced a clear reduction in splenomegaly and was equal in reducing antibody titles in comparison with MA. Our results demonstrated that MF is an effective and safe therapeutic alternative for leishmaniasis by L. (L.) amazonensis and is more efficacious than MA.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37136
Garcia Bustos, Maria Fernanda; Barrio, Alejandra; Prieto, Gabriela G.; De Raspi, Emma M.; Cimino, Rubén Oscar; et al.; In vivo antileishmanial efficacy of miltefosine against Leishmania (Leishmania) amazonensis; American Society of Parasitologists; Journal of Parasitology; 100; 6; 12-2014; 840-847
0022-3395
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37136
identifier_str_mv Garcia Bustos, Maria Fernanda; Barrio, Alejandra; Prieto, Gabriela G.; De Raspi, Emma M.; Cimino, Rubén Oscar; et al.; In vivo antileishmanial efficacy of miltefosine against Leishmania (Leishmania) amazonensis; American Society of Parasitologists; Journal of Parasitology; 100; 6; 12-2014; 840-847
0022-3395
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1645/13-376.1
info:eu-repo/semantics/altIdentifier/url/http://www.bioone.org/doi/abs/10.1645/13-376.1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Parasitologists
publisher.none.fl_str_mv American Society of Parasitologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268691939459072
score 13.13397

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