The eINTACT system dissects bacterial exploitation of plant osmosignalling to enhance virulence

Autores
You, Yuan; Koczyk, Grzegorz; Nuc, Maria; Morbitzer, Robert; Holmes, Danalyn R.; von Roepenack Lahaye, Edda; Hou, Shiji; Giudicatti, Axel Joel; Gris, Carine; Manavella, Pablo Andrés; Noël, Laurent D.; Krajewski, Paweł; Lahaye, Thomas
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bacteria inject effector proteins into host cells to manipulate cellular processes that promote disease. Since bacteria deliver minuscule amounts of effectors only into targeted host cells, it is technically challenging to capture effector-dependent cellular changes from bulk-infected host tissues. Here, we report a new technique called effector-inducible isolation of nuclei tagged in specific cell types (eINTACT), which facilitates affinity-based purification of nuclei from Arabidopsis plant cells that have received Xanthomonas bacterial effectors. Analysis of purified nuclei reveals that the Xanthomonas effector XopD manipulates the expression of Arabidopsis abscisic acid signalling-related genes and activates OSCA1.1, a gene encoding a calcium-permeable channel required for stomatal closure in response to osmotic stress. The loss of OSCA1.1 causes leaf wilting and reduced bacterial growth in infected leaves, suggesting that OSCA1.1 promotes host susceptibility. eINTACT allows us to uncover that XopD exploits host OSCA1.1/abscisic acid osmosignalling-mediated stomatal closure to create a humid habitat that favours bacterial growth and opens up a new avenue for accurately elucidating functions of effectors from numerous gram-negative plant bacteria in native infection contexts.
Fil: You, Yuan. Eberhard Karls Universität Tübingen; Alemania
Fil: Koczyk, Grzegorz. Polish Academy of Sciences; Argentina
Fil: Nuc, Maria. Polish Academy of Sciences; Argentina
Fil: Morbitzer, Robert. Eberhard Karls Universität Tübingen; Alemania
Fil: Holmes, Danalyn R.. Eberhard Karls Universität Tübingen; Alemania
Fil: von Roepenack Lahaye, Edda. Eberhard Karls Universität Tübingen; Alemania
Fil: Hou, Shiji. Huazhong Agricultural University; China
Fil: Giudicatti, Axel Joel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Gris, Carine. Université de Toulouse; Francia
Fil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Noël, Laurent D.. Université de Toulouse; Francia
Fil: Krajewski, Paweł. Polish Academy of Sciences; Argentina
Fil: Lahaye, Thomas. Eberhard Karls Universität Tübingen; Alemania
Materia
eINTACT
Arabidopsis thaliana
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/213731
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/213731
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The eINTACT system dissects bacterial exploitation of plant osmosignalling to enhance virulenceYou, YuanKoczyk, GrzegorzNuc, MariaMorbitzer, RobertHolmes, Danalyn R.von Roepenack Lahaye, EddaHou, ShijiGiudicatti, Axel JoelGris, CarineManavella, Pablo AndrésNoël, Laurent D.Krajewski, PawełLahaye, ThomaseINTACTArabidopsis thalianahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Bacteria inject effector proteins into host cells to manipulate cellular processes that promote disease. Since bacteria deliver minuscule amounts of effectors only into targeted host cells, it is technically challenging to capture effector-dependent cellular changes from bulk-infected host tissues. Here, we report a new technique called effector-inducible isolation of nuclei tagged in specific cell types (eINTACT), which facilitates affinity-based purification of nuclei from Arabidopsis plant cells that have received Xanthomonas bacterial effectors. Analysis of purified nuclei reveals that the Xanthomonas effector XopD manipulates the expression of Arabidopsis abscisic acid signalling-related genes and activates OSCA1.1, a gene encoding a calcium-permeable channel required for stomatal closure in response to osmotic stress. The loss of OSCA1.1 causes leaf wilting and reduced bacterial growth in infected leaves, suggesting that OSCA1.1 promotes host susceptibility. eINTACT allows us to uncover that XopD exploits host OSCA1.1/abscisic acid osmosignalling-mediated stomatal closure to create a humid habitat that favours bacterial growth and opens up a new avenue for accurately elucidating functions of effectors from numerous gram-negative plant bacteria in native infection contexts.Fil: You, Yuan. Eberhard Karls Universität Tübingen; AlemaniaFil: Koczyk, Grzegorz. Polish Academy of Sciences; ArgentinaFil: Nuc, Maria. Polish Academy of Sciences; ArgentinaFil: Morbitzer, Robert. Eberhard Karls Universität Tübingen; AlemaniaFil: Holmes, Danalyn R.. Eberhard Karls Universität Tübingen; AlemaniaFil: von Roepenack Lahaye, Edda. Eberhard Karls Universität Tübingen; AlemaniaFil: Hou, Shiji. Huazhong Agricultural University; ChinaFil: Giudicatti, Axel Joel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Gris, Carine. Université de Toulouse; FranciaFil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Noël, Laurent D.. Université de Toulouse; FranciaFil: Krajewski, Paweł. Polish Academy of Sciences; ArgentinaFil: Lahaye, Thomas. Eberhard Karls Universität Tübingen; AlemaniaSpringer2022-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/213731You, Yuan; Koczyk, Grzegorz; Nuc, Maria; Morbitzer, Robert; Holmes, Danalyn R.; et al.; The eINTACT system dissects bacterial exploitation of plant osmosignalling to enhance virulence; Springer; Nature Plants; 9; 1; 12-2022; 128-1412055-0278CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41477-022-01302-yinfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41477-022-01302-yinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:58Zoai:ri.conicet.gov.ar:11336/213731instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:58.43CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The eINTACT system dissects bacterial exploitation of plant osmosignalling to enhance virulence
title The eINTACT system dissects bacterial exploitation of plant osmosignalling to enhance virulence
spellingShingle The eINTACT system dissects bacterial exploitation of plant osmosignalling to enhance virulence
You, Yuan
eINTACT
Arabidopsis thaliana
title_short The eINTACT system dissects bacterial exploitation of plant osmosignalling to enhance virulence
title_full The eINTACT system dissects bacterial exploitation of plant osmosignalling to enhance virulence
title_fullStr The eINTACT system dissects bacterial exploitation of plant osmosignalling to enhance virulence
title_full_unstemmed The eINTACT system dissects bacterial exploitation of plant osmosignalling to enhance virulence
title_sort The eINTACT system dissects bacterial exploitation of plant osmosignalling to enhance virulence
dc.creator.none.fl_str_mv You, Yuan
Koczyk, Grzegorz
Nuc, Maria
Morbitzer, Robert
Holmes, Danalyn R.
von Roepenack Lahaye, Edda
Hou, Shiji
Giudicatti, Axel Joel
Gris, Carine
Manavella, Pablo Andrés
Noël, Laurent D.
Krajewski, Paweł
Lahaye, Thomas
author You, Yuan
author_facet You, Yuan
Koczyk, Grzegorz
Nuc, Maria
Morbitzer, Robert
Holmes, Danalyn R.
von Roepenack Lahaye, Edda
Hou, Shiji
Giudicatti, Axel Joel
Gris, Carine
Manavella, Pablo Andrés
Noël, Laurent D.
Krajewski, Paweł
Lahaye, Thomas
author_role author
author2 Koczyk, Grzegorz
Nuc, Maria
Morbitzer, Robert
Holmes, Danalyn R.
von Roepenack Lahaye, Edda
Hou, Shiji
Giudicatti, Axel Joel
Gris, Carine
Manavella, Pablo Andrés
Noël, Laurent D.
Krajewski, Paweł
Lahaye, Thomas
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv eINTACT
Arabidopsis thaliana
topic eINTACT
Arabidopsis thaliana
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bacteria inject effector proteins into host cells to manipulate cellular processes that promote disease. Since bacteria deliver minuscule amounts of effectors only into targeted host cells, it is technically challenging to capture effector-dependent cellular changes from bulk-infected host tissues. Here, we report a new technique called effector-inducible isolation of nuclei tagged in specific cell types (eINTACT), which facilitates affinity-based purification of nuclei from Arabidopsis plant cells that have received Xanthomonas bacterial effectors. Analysis of purified nuclei reveals that the Xanthomonas effector XopD manipulates the expression of Arabidopsis abscisic acid signalling-related genes and activates OSCA1.1, a gene encoding a calcium-permeable channel required for stomatal closure in response to osmotic stress. The loss of OSCA1.1 causes leaf wilting and reduced bacterial growth in infected leaves, suggesting that OSCA1.1 promotes host susceptibility. eINTACT allows us to uncover that XopD exploits host OSCA1.1/abscisic acid osmosignalling-mediated stomatal closure to create a humid habitat that favours bacterial growth and opens up a new avenue for accurately elucidating functions of effectors from numerous gram-negative plant bacteria in native infection contexts.
Fil: You, Yuan. Eberhard Karls Universität Tübingen; Alemania
Fil: Koczyk, Grzegorz. Polish Academy of Sciences; Argentina
Fil: Nuc, Maria. Polish Academy of Sciences; Argentina
Fil: Morbitzer, Robert. Eberhard Karls Universität Tübingen; Alemania
Fil: Holmes, Danalyn R.. Eberhard Karls Universität Tübingen; Alemania
Fil: von Roepenack Lahaye, Edda. Eberhard Karls Universität Tübingen; Alemania
Fil: Hou, Shiji. Huazhong Agricultural University; China
Fil: Giudicatti, Axel Joel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Gris, Carine. Université de Toulouse; Francia
Fil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Noël, Laurent D.. Université de Toulouse; Francia
Fil: Krajewski, Paweł. Polish Academy of Sciences; Argentina
Fil: Lahaye, Thomas. Eberhard Karls Universität Tübingen; Alemania
description Bacteria inject effector proteins into host cells to manipulate cellular processes that promote disease. Since bacteria deliver minuscule amounts of effectors only into targeted host cells, it is technically challenging to capture effector-dependent cellular changes from bulk-infected host tissues. Here, we report a new technique called effector-inducible isolation of nuclei tagged in specific cell types (eINTACT), which facilitates affinity-based purification of nuclei from Arabidopsis plant cells that have received Xanthomonas bacterial effectors. Analysis of purified nuclei reveals that the Xanthomonas effector XopD manipulates the expression of Arabidopsis abscisic acid signalling-related genes and activates OSCA1.1, a gene encoding a calcium-permeable channel required for stomatal closure in response to osmotic stress. The loss of OSCA1.1 causes leaf wilting and reduced bacterial growth in infected leaves, suggesting that OSCA1.1 promotes host susceptibility. eINTACT allows us to uncover that XopD exploits host OSCA1.1/abscisic acid osmosignalling-mediated stomatal closure to create a humid habitat that favours bacterial growth and opens up a new avenue for accurately elucidating functions of effectors from numerous gram-negative plant bacteria in native infection contexts.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/213731
You, Yuan; Koczyk, Grzegorz; Nuc, Maria; Morbitzer, Robert; Holmes, Danalyn R.; et al.; The eINTACT system dissects bacterial exploitation of plant osmosignalling to enhance virulence; Springer; Nature Plants; 9; 1; 12-2022; 128-141
2055-0278
CONICET Digital
CONICET
url http://hdl.handle.net/11336/213731
identifier_str_mv You, Yuan; Koczyk, Grzegorz; Nuc, Maria; Morbitzer, Robert; Holmes, Danalyn R.; et al.; The eINTACT system dissects bacterial exploitation of plant osmosignalling to enhance virulence; Springer; Nature Plants; 9; 1; 12-2022; 128-141
2055-0278
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41477-022-01302-y
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41477-022-01302-y
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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