S-layer production by Lactobacillus acidophilus IBB 801 under environmental stress conditions

Autores
Grosu Tudor, Silvia Simona; Brown, Lucia; Hebert, Elvira Maria; Brezeanu, Aurelia; Brinzan, Alexandru; Fadda, Silvina G.; Mozzi, Fernanda Beatriz; Zamfir, Medana
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The ability of microorganisms to synthesize S-layer, the outermost structure of the microbial cell envelope composed of non-covalently bound proteins, has been ascribed to help microorganisms to exert their probiotic properties in the host. In this work, formation of S-layer by the potentially probiotic strain Lactobacillus acidophilus IBB 801 under different stress culture conditions (high incubation temperatures, presence of bile salts or NaCl, and acidic pH) was assayed. A marked S-layer synthesis by L. acidophilus IBB 801 was detected when the strain was grown at 42 °C and in the presence of 0.05 % bile salts or 2.0 % NaCl. The presence of S-layer proteins was further confirmed by transmission electron microscopy and protein identification by MS/MS. The differential expression of the proteome of this strain at 42 °C, when a marked formation of S-layer was detected, revealed the overexpression of six proteins mainly related to general stress and protein biosynthesis and translation, while four proteins detected in lower amounts were involved in DNA repair and energy metabolism. As L. acidophilus IBB 801 produces both a bacteriocin and S-layer proteins, the strain could be of interest to be used in the formulation of functional food products with specific properties.
Fil: Grosu Tudor, Silvia Simona. Academia Romana, Institutul de Biologie Bucuresti; Rumania
Fil: Brown, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Brezeanu, Aurelia. Academia Romana, Institutul de Biologie Bucuresti; Rumania
Fil: Brinzan, Alexandru. Academia Romana, Institutul de Biologie Bucuresti; Rumania
Fil: Fadda, Silvina G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Mozzi, Fernanda Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Zamfir, Medana. Academia Romana, Institutul de Biologie Bucuresti; Rumania
Materia
Lactobacilli
Proteomics
S-Layer Proteins
Stress
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/39295
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/39295
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling S-layer production by Lactobacillus acidophilus IBB 801 under environmental stress conditionsGrosu Tudor, Silvia SimonaBrown, LuciaHebert, Elvira MariaBrezeanu, AureliaBrinzan, AlexandruFadda, Silvina G.Mozzi, Fernanda BeatrizZamfir, MedanaLactobacilliProteomicsS-Layer ProteinsStresshttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The ability of microorganisms to synthesize S-layer, the outermost structure of the microbial cell envelope composed of non-covalently bound proteins, has been ascribed to help microorganisms to exert their probiotic properties in the host. In this work, formation of S-layer by the potentially probiotic strain Lactobacillus acidophilus IBB 801 under different stress culture conditions (high incubation temperatures, presence of bile salts or NaCl, and acidic pH) was assayed. A marked S-layer synthesis by L. acidophilus IBB 801 was detected when the strain was grown at 42 °C and in the presence of 0.05 % bile salts or 2.0 % NaCl. The presence of S-layer proteins was further confirmed by transmission electron microscopy and protein identification by MS/MS. The differential expression of the proteome of this strain at 42 °C, when a marked formation of S-layer was detected, revealed the overexpression of six proteins mainly related to general stress and protein biosynthesis and translation, while four proteins detected in lower amounts were involved in DNA repair and energy metabolism. As L. acidophilus IBB 801 produces both a bacteriocin and S-layer proteins, the strain could be of interest to be used in the formulation of functional food products with specific properties.Fil: Grosu Tudor, Silvia Simona. Academia Romana, Institutul de Biologie Bucuresti; RumaniaFil: Brown, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Brezeanu, Aurelia. Academia Romana, Institutul de Biologie Bucuresti; RumaniaFil: Brinzan, Alexandru. Academia Romana, Institutul de Biologie Bucuresti; RumaniaFil: Fadda, Silvina G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Mozzi, Fernanda Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Zamfir, Medana. Academia Romana, Institutul de Biologie Bucuresti; RumaniaSpringer2016-05-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39295Grosu Tudor, Silvia Simona; Brown, Lucia; Hebert, Elvira Maria; Brezeanu, Aurelia; Brinzan, Alexandru; et al.; S-layer production by Lactobacillus acidophilus IBB 801 under environmental stress conditions; Springer; Applied Microbiology and Biotechnology; 100; 10; 24-5-2016; 4573-45830175-7598CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00253-016-7355-5info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00253-016-7355-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:18:02Zoai:ri.conicet.gov.ar:11336/39295instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:18:02.764CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv S-layer production by Lactobacillus acidophilus IBB 801 under environmental stress conditions
title S-layer production by Lactobacillus acidophilus IBB 801 under environmental stress conditions
spellingShingle S-layer production by Lactobacillus acidophilus IBB 801 under environmental stress conditions
Grosu Tudor, Silvia Simona
Lactobacilli
Proteomics
S-Layer Proteins
Stress
title_short S-layer production by Lactobacillus acidophilus IBB 801 under environmental stress conditions
title_full S-layer production by Lactobacillus acidophilus IBB 801 under environmental stress conditions
title_fullStr S-layer production by Lactobacillus acidophilus IBB 801 under environmental stress conditions
title_full_unstemmed S-layer production by Lactobacillus acidophilus IBB 801 under environmental stress conditions
title_sort S-layer production by Lactobacillus acidophilus IBB 801 under environmental stress conditions
dc.creator.none.fl_str_mv Grosu Tudor, Silvia Simona
Brown, Lucia
Hebert, Elvira Maria
Brezeanu, Aurelia
Brinzan, Alexandru
Fadda, Silvina G.
Mozzi, Fernanda Beatriz
Zamfir, Medana
author Grosu Tudor, Silvia Simona
author_facet Grosu Tudor, Silvia Simona
Brown, Lucia
Hebert, Elvira Maria
Brezeanu, Aurelia
Brinzan, Alexandru
Fadda, Silvina G.
Mozzi, Fernanda Beatriz
Zamfir, Medana
author_role author
author2 Brown, Lucia
Hebert, Elvira Maria
Brezeanu, Aurelia
Brinzan, Alexandru
Fadda, Silvina G.
Mozzi, Fernanda Beatriz
Zamfir, Medana
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Lactobacilli
Proteomics
S-Layer Proteins
Stress
topic Lactobacilli
Proteomics
S-Layer Proteins
Stress
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The ability of microorganisms to synthesize S-layer, the outermost structure of the microbial cell envelope composed of non-covalently bound proteins, has been ascribed to help microorganisms to exert their probiotic properties in the host. In this work, formation of S-layer by the potentially probiotic strain Lactobacillus acidophilus IBB 801 under different stress culture conditions (high incubation temperatures, presence of bile salts or NaCl, and acidic pH) was assayed. A marked S-layer synthesis by L. acidophilus IBB 801 was detected when the strain was grown at 42 °C and in the presence of 0.05 % bile salts or 2.0 % NaCl. The presence of S-layer proteins was further confirmed by transmission electron microscopy and protein identification by MS/MS. The differential expression of the proteome of this strain at 42 °C, when a marked formation of S-layer was detected, revealed the overexpression of six proteins mainly related to general stress and protein biosynthesis and translation, while four proteins detected in lower amounts were involved in DNA repair and energy metabolism. As L. acidophilus IBB 801 produces both a bacteriocin and S-layer proteins, the strain could be of interest to be used in the formulation of functional food products with specific properties.
Fil: Grosu Tudor, Silvia Simona. Academia Romana, Institutul de Biologie Bucuresti; Rumania
Fil: Brown, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Brezeanu, Aurelia. Academia Romana, Institutul de Biologie Bucuresti; Rumania
Fil: Brinzan, Alexandru. Academia Romana, Institutul de Biologie Bucuresti; Rumania
Fil: Fadda, Silvina G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Mozzi, Fernanda Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Zamfir, Medana. Academia Romana, Institutul de Biologie Bucuresti; Rumania
description The ability of microorganisms to synthesize S-layer, the outermost structure of the microbial cell envelope composed of non-covalently bound proteins, has been ascribed to help microorganisms to exert their probiotic properties in the host. In this work, formation of S-layer by the potentially probiotic strain Lactobacillus acidophilus IBB 801 under different stress culture conditions (high incubation temperatures, presence of bile salts or NaCl, and acidic pH) was assayed. A marked S-layer synthesis by L. acidophilus IBB 801 was detected when the strain was grown at 42 °C and in the presence of 0.05 % bile salts or 2.0 % NaCl. The presence of S-layer proteins was further confirmed by transmission electron microscopy and protein identification by MS/MS. The differential expression of the proteome of this strain at 42 °C, when a marked formation of S-layer was detected, revealed the overexpression of six proteins mainly related to general stress and protein biosynthesis and translation, while four proteins detected in lower amounts were involved in DNA repair and energy metabolism. As L. acidophilus IBB 801 produces both a bacteriocin and S-layer proteins, the strain could be of interest to be used in the formulation of functional food products with specific properties.
publishDate 2016
dc.date.none.fl_str_mv 2016-05-24
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39295
Grosu Tudor, Silvia Simona; Brown, Lucia; Hebert, Elvira Maria; Brezeanu, Aurelia; Brinzan, Alexandru; et al.; S-layer production by Lactobacillus acidophilus IBB 801 under environmental stress conditions; Springer; Applied Microbiology and Biotechnology; 100; 10; 24-5-2016; 4573-4583
0175-7598
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39295
identifier_str_mv Grosu Tudor, Silvia Simona; Brown, Lucia; Hebert, Elvira Maria; Brezeanu, Aurelia; Brinzan, Alexandru; et al.; S-layer production by Lactobacillus acidophilus IBB 801 under environmental stress conditions; Springer; Applied Microbiology and Biotechnology; 100; 10; 24-5-2016; 4573-4583
0175-7598
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00253-016-7355-5
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00253-016-7355-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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