A neuropeptide screen to identify modulators of behavioral alterations induced by confinement

Autores
Ibarra, Julieta; Heredia, Fabiana; Zanini, Rebeca; Varela, Ednilson; Carreira, Olga; Menezes, Juliane; Macedo, Andre; Gontijo, Alisson; Garelli, Andres
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The relevance of the word “confinement” and the negative effects it has on physical, mental, and emotional wellbeing has become particularly evident to humanity during the COVID-19 pandemic, when mandatory or fear-induced self-confinements were highly effective in reducing viral spread, but they also severely disrupted human behavior. The molecular and neural mechanisms underlying the translation of physical confinement to internal state changes and how these alter behavior are poorly understood and very difficult to study in natural settings due to an excessive number of possible confounding factors. Innate behaviors, which are complex, genetically-encoded behaviors that proceed with a predictable sequence, provide a unique window to study the effects of confinement on behavior. We used the wing expansion behavior that Drosophila juveniles perform upon eclosing from their puparium to unravel both the molecular and cellular correlates of behaviorally-relevant, confinementinduced changes in internal states. Newly emerged flies select a suitable place to perch and initiate a stereotyped behavior that leads to the expansion of the folded wings. The execution of this behavior, which is triggered by the hormone Bursicon, is strongly negatively-regulated by spatial confinement. Placement of flies in small chambers extends the perch selection phase, but the environmental cues, neural circuits and molecular mechanism that mediate this decision remain unknown. Here, we describe our experimental setup and present the preliminary results of our pan neuronal RNAi silencing screen devised to test the requirement of neuropeptides and neurotransmitters in this behavioral choice.
Fil: Ibarra, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Heredia, Fabiana. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Zanini, Rebeca. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Varela, Ednilson. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Carreira, Olga. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Menezes, Juliane. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Macedo, Andre. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Gontijo, Alisson. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Garelli, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
V Taller de Biología Celular y del Desarrollo
Chascomús
Argentina
Comité Organizador del V Taller de Biología Celular y del Desarrollo
Materia
DROSOPHILA
CONFINEMENT
RELAXINS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/205974
Acceder
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network_name_str CONICET Digital (CONICET)
spelling A neuropeptide screen to identify modulators of behavioral alterations induced by confinementIbarra, JulietaHeredia, FabianaZanini, RebecaVarela, EdnilsonCarreira, OlgaMenezes, JulianeMacedo, AndreGontijo, AlissonGarelli, AndresDROSOPHILACONFINEMENTRELAXINShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The relevance of the word “confinement” and the negative effects it has on physical, mental, and emotional wellbeing has become particularly evident to humanity during the COVID-19 pandemic, when mandatory or fear-induced self-confinements were highly effective in reducing viral spread, but they also severely disrupted human behavior. The molecular and neural mechanisms underlying the translation of physical confinement to internal state changes and how these alter behavior are poorly understood and very difficult to study in natural settings due to an excessive number of possible confounding factors. Innate behaviors, which are complex, genetically-encoded behaviors that proceed with a predictable sequence, provide a unique window to study the effects of confinement on behavior. We used the wing expansion behavior that Drosophila juveniles perform upon eclosing from their puparium to unravel both the molecular and cellular correlates of behaviorally-relevant, confinementinduced changes in internal states. Newly emerged flies select a suitable place to perch and initiate a stereotyped behavior that leads to the expansion of the folded wings. The execution of this behavior, which is triggered by the hormone Bursicon, is strongly negatively-regulated by spatial confinement. Placement of flies in small chambers extends the perch selection phase, but the environmental cues, neural circuits and molecular mechanism that mediate this decision remain unknown. Here, we describe our experimental setup and present the preliminary results of our pan neuronal RNAi silencing screen devised to test the requirement of neuropeptides and neurotransmitters in this behavioral choice.Fil: Ibarra, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Heredia, Fabiana. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Zanini, Rebeca. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Varela, Ednilson. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Carreira, Olga. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Menezes, Juliane. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Macedo, Andre. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Gontijo, Alisson. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Garelli, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaV Taller de Biología Celular y del DesarrolloChascomúsArgentinaComité Organizador del V Taller de Biología Celular y del DesarrolloComité Organizador del V Taller de Biología Celular y del Desarrollo2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectTallerBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/205974A neuropeptide screen to identify modulators of behavioral alterations induced by confinement; V Taller de Biología Celular y del Desarrollo; Chascomús; Argentina; 2022; 29-30CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://tallerbcd.wixsite.com/tallerbcdNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-06T12:21:53Zoai:ri.conicet.gov.ar:11336/205974instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-06 12:21:54.07CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A neuropeptide screen to identify modulators of behavioral alterations induced by confinement
title A neuropeptide screen to identify modulators of behavioral alterations induced by confinement
spellingShingle A neuropeptide screen to identify modulators of behavioral alterations induced by confinement
Ibarra, Julieta
DROSOPHILA
CONFINEMENT
RELAXINS
title_short A neuropeptide screen to identify modulators of behavioral alterations induced by confinement
title_full A neuropeptide screen to identify modulators of behavioral alterations induced by confinement
title_fullStr A neuropeptide screen to identify modulators of behavioral alterations induced by confinement
title_full_unstemmed A neuropeptide screen to identify modulators of behavioral alterations induced by confinement
title_sort A neuropeptide screen to identify modulators of behavioral alterations induced by confinement
dc.creator.none.fl_str_mv Ibarra, Julieta
Heredia, Fabiana
Zanini, Rebeca
Varela, Ednilson
Carreira, Olga
Menezes, Juliane
Macedo, Andre
Gontijo, Alisson
Garelli, Andres
author Ibarra, Julieta
author_facet Ibarra, Julieta
Heredia, Fabiana
Zanini, Rebeca
Varela, Ednilson
Carreira, Olga
Menezes, Juliane
Macedo, Andre
Gontijo, Alisson
Garelli, Andres
author_role author
author2 Heredia, Fabiana
Zanini, Rebeca
Varela, Ednilson
Carreira, Olga
Menezes, Juliane
Macedo, Andre
Gontijo, Alisson
Garelli, Andres
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DROSOPHILA
CONFINEMENT
RELAXINS
topic DROSOPHILA
CONFINEMENT
RELAXINS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The relevance of the word “confinement” and the negative effects it has on physical, mental, and emotional wellbeing has become particularly evident to humanity during the COVID-19 pandemic, when mandatory or fear-induced self-confinements were highly effective in reducing viral spread, but they also severely disrupted human behavior. The molecular and neural mechanisms underlying the translation of physical confinement to internal state changes and how these alter behavior are poorly understood and very difficult to study in natural settings due to an excessive number of possible confounding factors. Innate behaviors, which are complex, genetically-encoded behaviors that proceed with a predictable sequence, provide a unique window to study the effects of confinement on behavior. We used the wing expansion behavior that Drosophila juveniles perform upon eclosing from their puparium to unravel both the molecular and cellular correlates of behaviorally-relevant, confinementinduced changes in internal states. Newly emerged flies select a suitable place to perch and initiate a stereotyped behavior that leads to the expansion of the folded wings. The execution of this behavior, which is triggered by the hormone Bursicon, is strongly negatively-regulated by spatial confinement. Placement of flies in small chambers extends the perch selection phase, but the environmental cues, neural circuits and molecular mechanism that mediate this decision remain unknown. Here, we describe our experimental setup and present the preliminary results of our pan neuronal RNAi silencing screen devised to test the requirement of neuropeptides and neurotransmitters in this behavioral choice.
Fil: Ibarra, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Heredia, Fabiana. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Zanini, Rebeca. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Varela, Ednilson. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Carreira, Olga. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Menezes, Juliane. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Macedo, Andre. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Gontijo, Alisson. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Garelli, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
V Taller de Biología Celular y del Desarrollo
Chascomús
Argentina
Comité Organizador del V Taller de Biología Celular y del Desarrollo
description The relevance of the word “confinement” and the negative effects it has on physical, mental, and emotional wellbeing has become particularly evident to humanity during the COVID-19 pandemic, when mandatory or fear-induced self-confinements were highly effective in reducing viral spread, but they also severely disrupted human behavior. The molecular and neural mechanisms underlying the translation of physical confinement to internal state changes and how these alter behavior are poorly understood and very difficult to study in natural settings due to an excessive number of possible confounding factors. Innate behaviors, which are complex, genetically-encoded behaviors that proceed with a predictable sequence, provide a unique window to study the effects of confinement on behavior. We used the wing expansion behavior that Drosophila juveniles perform upon eclosing from their puparium to unravel both the molecular and cellular correlates of behaviorally-relevant, confinementinduced changes in internal states. Newly emerged flies select a suitable place to perch and initiate a stereotyped behavior that leads to the expansion of the folded wings. The execution of this behavior, which is triggered by the hormone Bursicon, is strongly negatively-regulated by spatial confinement. Placement of flies in small chambers extends the perch selection phase, but the environmental cues, neural circuits and molecular mechanism that mediate this decision remain unknown. Here, we describe our experimental setup and present the preliminary results of our pan neuronal RNAi silencing screen devised to test the requirement of neuropeptides and neurotransmitters in this behavioral choice.
publishDate 2022
dc.date.none.fl_str_mv 2022
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info:eu-repo/semantics/conferenceObject
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Book
http://purl.org/coar/resource_type/c_5794
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dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/205974
A neuropeptide screen to identify modulators of behavioral alterations induced by confinement; V Taller de Biología Celular y del Desarrollo; Chascomús; Argentina; 2022; 29-30
CONICET Digital
CONICET
url http://hdl.handle.net/11336/205974
identifier_str_mv A neuropeptide screen to identify modulators of behavioral alterations induced by confinement; V Taller de Biología Celular y del Desarrollo; Chascomús; Argentina; 2022; 29-30
CONICET Digital
CONICET
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language eng
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Comité Organizador del V Taller de Biología Celular y del Desarrollo
publisher.none.fl_str_mv Comité Organizador del V Taller de Biología Celular y del Desarrollo
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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