Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry

Autores
Scursoni, Alejandra M.; Galluzzo, Laura; Camarero, Sandra; Pozzo, Norma; Gabri, Mariano Rolando; De Acosta, Cristina Mateo; Vázquez, Ana María; Alonso, Daniel Fernando; De Davila, María Teresa G.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Gangliosides are glycolipids present on the cell surface. The N-glycolylated ganglioside NeuGc-GM3 has been described in some neoplasms, such as breast carcinoma and melanoma, but is usually not detected in normal human cells. Our aim was to evaluate the presence of NeuGc-GM3 in Wilms tumor by immunohistochemistry. Postchemotherapy tumors were grouped into different histologic subtypes considering the main preserved component. Formalin-fixed, paraffin-embedded tumor samples were cut into 5-mm sections. The monoclonal antibody 14F7, a mouse IgG1 that specifically recognizes NeuGc-GM3, and a peroxidase-labeled polymer conjugated to secondary antibodies were used. Sections from breast carcinoma were employed as positive controls. Presence of NeuGc-GM3 was evident in 22 of 25 (88%) cases. The staining was stronger in the epithelial component, with a membrane pattern and cytoplasmic diffusion. The stromal component expressed cytoplasmic NeuGc-GM3 in cells with rhabdomyoblastic differentiation. Tubules of adjacent renal tissue were also positive, but no expression of NeuGc-GM3 was detected in nontumoral fetal kidney. Until now, the expression of N-glycolylated gangliosides in pediatric solid tumors has not been investigated. The present study evidenced the expression of NeuGc-GM3 in a high proportion of Wilms tumors, suggesting its potential utility as a specific target of immunotherapy.
Fil: Scursoni, Alejandra M.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina
Fil: Galluzzo, Laura. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina
Fil: Camarero, Sandra. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina
Fil: Pozzo, Norma. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina
Fil: Gabri, Mariano Rolando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De Acosta, Cristina Mateo. Centro de Inmunología Molecular; Cuba
Fil: Vázquez, Ana María. Centro de Inmunología Molecular; Cuba
Fil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De Davila, María Teresa G.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina
Materia
Gangliosides
Gm3
Immunohistochemistry
Immunotherapy
N-Glycolyl Neuraminic Acid
Nephroblastoma
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54525
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/54525
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistryScursoni, Alejandra M.Galluzzo, LauraCamarero, SandraPozzo, NormaGabri, Mariano RolandoDe Acosta, Cristina MateoVázquez, Ana MaríaAlonso, Daniel FernandoDe Davila, María Teresa G.GangliosidesGm3ImmunohistochemistryImmunotherapyN-Glycolyl Neuraminic AcidNephroblastomahttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Gangliosides are glycolipids present on the cell surface. The N-glycolylated ganglioside NeuGc-GM3 has been described in some neoplasms, such as breast carcinoma and melanoma, but is usually not detected in normal human cells. Our aim was to evaluate the presence of NeuGc-GM3 in Wilms tumor by immunohistochemistry. Postchemotherapy tumors were grouped into different histologic subtypes considering the main preserved component. Formalin-fixed, paraffin-embedded tumor samples were cut into 5-mm sections. The monoclonal antibody 14F7, a mouse IgG1 that specifically recognizes NeuGc-GM3, and a peroxidase-labeled polymer conjugated to secondary antibodies were used. Sections from breast carcinoma were employed as positive controls. Presence of NeuGc-GM3 was evident in 22 of 25 (88%) cases. The staining was stronger in the epithelial component, with a membrane pattern and cytoplasmic diffusion. The stromal component expressed cytoplasmic NeuGc-GM3 in cells with rhabdomyoblastic differentiation. Tubules of adjacent renal tissue were also positive, but no expression of NeuGc-GM3 was detected in nontumoral fetal kidney. Until now, the expression of N-glycolylated gangliosides in pediatric solid tumors has not been investigated. The present study evidenced the expression of NeuGc-GM3 in a high proportion of Wilms tumors, suggesting its potential utility as a specific target of immunotherapy.Fil: Scursoni, Alejandra M.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Galluzzo, Laura. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Camarero, Sandra. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Pozzo, Norma. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Gabri, Mariano Rolando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: De Acosta, Cristina Mateo. Centro de Inmunología Molecular; CubaFil: Vázquez, Ana María. Centro de Inmunología Molecular; CubaFil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: De Davila, María Teresa G.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaAlliance Communications Group Division Allen Press2010-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54525Scursoni, Alejandra M.; Galluzzo, Laura; Camarero, Sandra; Pozzo, Norma; Gabri, Mariano Rolando; et al.; Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry; Alliance Communications Group Division Allen Press; Pediatric And Developmental Pathology; 13; 1; 1-2010; 18-231093-5266CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/abs/10.2350/08-10-0544.1info:eu-repo/semantics/altIdentifier/doi/10.2350/08-10-0544.1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:39:11Zoai:ri.conicet.gov.ar:11336/54525instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:11.813CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry
title Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry
spellingShingle Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry
Scursoni, Alejandra M.
Gangliosides
Gm3
Immunohistochemistry
Immunotherapy
N-Glycolyl Neuraminic Acid
Nephroblastoma
title_short Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry
title_full Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry
title_fullStr Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry
title_full_unstemmed Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry
title_sort Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry
dc.creator.none.fl_str_mv Scursoni, Alejandra M.
Galluzzo, Laura
Camarero, Sandra
Pozzo, Norma
Gabri, Mariano Rolando
De Acosta, Cristina Mateo
Vázquez, Ana María
Alonso, Daniel Fernando
De Davila, María Teresa G.
author Scursoni, Alejandra M.
author_facet Scursoni, Alejandra M.
Galluzzo, Laura
Camarero, Sandra
Pozzo, Norma
Gabri, Mariano Rolando
De Acosta, Cristina Mateo
Vázquez, Ana María
Alonso, Daniel Fernando
De Davila, María Teresa G.
author_role author
author2 Galluzzo, Laura
Camarero, Sandra
Pozzo, Norma
Gabri, Mariano Rolando
De Acosta, Cristina Mateo
Vázquez, Ana María
Alonso, Daniel Fernando
De Davila, María Teresa G.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Gangliosides
Gm3
Immunohistochemistry
Immunotherapy
N-Glycolyl Neuraminic Acid
Nephroblastoma
topic Gangliosides
Gm3
Immunohistochemistry
Immunotherapy
N-Glycolyl Neuraminic Acid
Nephroblastoma
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Gangliosides are glycolipids present on the cell surface. The N-glycolylated ganglioside NeuGc-GM3 has been described in some neoplasms, such as breast carcinoma and melanoma, but is usually not detected in normal human cells. Our aim was to evaluate the presence of NeuGc-GM3 in Wilms tumor by immunohistochemistry. Postchemotherapy tumors were grouped into different histologic subtypes considering the main preserved component. Formalin-fixed, paraffin-embedded tumor samples were cut into 5-mm sections. The monoclonal antibody 14F7, a mouse IgG1 that specifically recognizes NeuGc-GM3, and a peroxidase-labeled polymer conjugated to secondary antibodies were used. Sections from breast carcinoma were employed as positive controls. Presence of NeuGc-GM3 was evident in 22 of 25 (88%) cases. The staining was stronger in the epithelial component, with a membrane pattern and cytoplasmic diffusion. The stromal component expressed cytoplasmic NeuGc-GM3 in cells with rhabdomyoblastic differentiation. Tubules of adjacent renal tissue were also positive, but no expression of NeuGc-GM3 was detected in nontumoral fetal kidney. Until now, the expression of N-glycolylated gangliosides in pediatric solid tumors has not been investigated. The present study evidenced the expression of NeuGc-GM3 in a high proportion of Wilms tumors, suggesting its potential utility as a specific target of immunotherapy.
Fil: Scursoni, Alejandra M.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina
Fil: Galluzzo, Laura. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina
Fil: Camarero, Sandra. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina
Fil: Pozzo, Norma. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina
Fil: Gabri, Mariano Rolando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De Acosta, Cristina Mateo. Centro de Inmunología Molecular; Cuba
Fil: Vázquez, Ana María. Centro de Inmunología Molecular; Cuba
Fil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De Davila, María Teresa G.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina
description Gangliosides are glycolipids present on the cell surface. The N-glycolylated ganglioside NeuGc-GM3 has been described in some neoplasms, such as breast carcinoma and melanoma, but is usually not detected in normal human cells. Our aim was to evaluate the presence of NeuGc-GM3 in Wilms tumor by immunohistochemistry. Postchemotherapy tumors were grouped into different histologic subtypes considering the main preserved component. Formalin-fixed, paraffin-embedded tumor samples were cut into 5-mm sections. The monoclonal antibody 14F7, a mouse IgG1 that specifically recognizes NeuGc-GM3, and a peroxidase-labeled polymer conjugated to secondary antibodies were used. Sections from breast carcinoma were employed as positive controls. Presence of NeuGc-GM3 was evident in 22 of 25 (88%) cases. The staining was stronger in the epithelial component, with a membrane pattern and cytoplasmic diffusion. The stromal component expressed cytoplasmic NeuGc-GM3 in cells with rhabdomyoblastic differentiation. Tubules of adjacent renal tissue were also positive, but no expression of NeuGc-GM3 was detected in nontumoral fetal kidney. Until now, the expression of N-glycolylated gangliosides in pediatric solid tumors has not been investigated. The present study evidenced the expression of NeuGc-GM3 in a high proportion of Wilms tumors, suggesting its potential utility as a specific target of immunotherapy.
publishDate 2010
dc.date.none.fl_str_mv 2010-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54525
Scursoni, Alejandra M.; Galluzzo, Laura; Camarero, Sandra; Pozzo, Norma; Gabri, Mariano Rolando; et al.; Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry; Alliance Communications Group Division Allen Press; Pediatric And Developmental Pathology; 13; 1; 1-2010; 18-23
1093-5266
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54525
identifier_str_mv Scursoni, Alejandra M.; Galluzzo, Laura; Camarero, Sandra; Pozzo, Norma; Gabri, Mariano Rolando; et al.; Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry; Alliance Communications Group Division Allen Press; Pediatric And Developmental Pathology; 13; 1; 1-2010; 18-23
1093-5266
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/abs/10.2350/08-10-0544.1
info:eu-repo/semantics/altIdentifier/doi/10.2350/08-10-0544.1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Alliance Communications Group Division Allen Press
publisher.none.fl_str_mv Alliance Communications Group Division Allen Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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