Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry
- Autores
- Scursoni, Alejandra M.; Galluzzo, Laura; Camarero, Sandra; Pozzo, Norma; Gabri, Mariano Rolando; De Acosta, Cristina Mateo; Vázquez, Ana María; Alonso, Daniel Fernando; De Davila, María Teresa G.
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Gangliosides are glycolipids present on the cell surface. The N-glycolylated ganglioside NeuGc-GM3 has been described in some neoplasms, such as breast carcinoma and melanoma, but is usually not detected in normal human cells. Our aim was to evaluate the presence of NeuGc-GM3 in Wilms tumor by immunohistochemistry. Postchemotherapy tumors were grouped into different histologic subtypes considering the main preserved component. Formalin-fixed, paraffin-embedded tumor samples were cut into 5-mm sections. The monoclonal antibody 14F7, a mouse IgG1 that specifically recognizes NeuGc-GM3, and a peroxidase-labeled polymer conjugated to secondary antibodies were used. Sections from breast carcinoma were employed as positive controls. Presence of NeuGc-GM3 was evident in 22 of 25 (88%) cases. The staining was stronger in the epithelial component, with a membrane pattern and cytoplasmic diffusion. The stromal component expressed cytoplasmic NeuGc-GM3 in cells with rhabdomyoblastic differentiation. Tubules of adjacent renal tissue were also positive, but no expression of NeuGc-GM3 was detected in nontumoral fetal kidney. Until now, the expression of N-glycolylated gangliosides in pediatric solid tumors has not been investigated. The present study evidenced the expression of NeuGc-GM3 in a high proportion of Wilms tumors, suggesting its potential utility as a specific target of immunotherapy.
Fil: Scursoni, Alejandra M.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina
Fil: Galluzzo, Laura. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina
Fil: Camarero, Sandra. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina
Fil: Pozzo, Norma. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina
Fil: Gabri, Mariano Rolando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De Acosta, Cristina Mateo. Centro de Inmunología Molecular; Cuba
Fil: Vázquez, Ana María. Centro de Inmunología Molecular; Cuba
Fil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De Davila, María Teresa G.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina - Materia
-
Gangliosides
Gm3
Immunohistochemistry
Immunotherapy
N-Glycolyl Neuraminic Acid
Nephroblastoma - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/54525
Ver los metadatos del registro completo
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Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistryScursoni, Alejandra M.Galluzzo, LauraCamarero, SandraPozzo, NormaGabri, Mariano RolandoDe Acosta, Cristina MateoVázquez, Ana MaríaAlonso, Daniel FernandoDe Davila, María Teresa G.GangliosidesGm3ImmunohistochemistryImmunotherapyN-Glycolyl Neuraminic AcidNephroblastomahttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Gangliosides are glycolipids present on the cell surface. The N-glycolylated ganglioside NeuGc-GM3 has been described in some neoplasms, such as breast carcinoma and melanoma, but is usually not detected in normal human cells. Our aim was to evaluate the presence of NeuGc-GM3 in Wilms tumor by immunohistochemistry. Postchemotherapy tumors were grouped into different histologic subtypes considering the main preserved component. Formalin-fixed, paraffin-embedded tumor samples were cut into 5-mm sections. The monoclonal antibody 14F7, a mouse IgG1 that specifically recognizes NeuGc-GM3, and a peroxidase-labeled polymer conjugated to secondary antibodies were used. Sections from breast carcinoma were employed as positive controls. Presence of NeuGc-GM3 was evident in 22 of 25 (88%) cases. The staining was stronger in the epithelial component, with a membrane pattern and cytoplasmic diffusion. The stromal component expressed cytoplasmic NeuGc-GM3 in cells with rhabdomyoblastic differentiation. Tubules of adjacent renal tissue were also positive, but no expression of NeuGc-GM3 was detected in nontumoral fetal kidney. Until now, the expression of N-glycolylated gangliosides in pediatric solid tumors has not been investigated. The present study evidenced the expression of NeuGc-GM3 in a high proportion of Wilms tumors, suggesting its potential utility as a specific target of immunotherapy.Fil: Scursoni, Alejandra M.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Galluzzo, Laura. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Camarero, Sandra. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Pozzo, Norma. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Gabri, Mariano Rolando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: De Acosta, Cristina Mateo. Centro de Inmunología Molecular; CubaFil: Vázquez, Ana María. Centro de Inmunología Molecular; CubaFil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: De Davila, María Teresa G.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaAlliance Communications Group Division Allen Press2010-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54525Scursoni, Alejandra M.; Galluzzo, Laura; Camarero, Sandra; Pozzo, Norma; Gabri, Mariano Rolando; et al.; Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry; Alliance Communications Group Division Allen Press; Pediatric And Developmental Pathology; 13; 1; 1-2010; 18-231093-5266CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/abs/10.2350/08-10-0544.1info:eu-repo/semantics/altIdentifier/doi/10.2350/08-10-0544.1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:17Zoai:ri.conicet.gov.ar:11336/54525instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:17.505CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry |
title |
Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry |
spellingShingle |
Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry Scursoni, Alejandra M. Gangliosides Gm3 Immunohistochemistry Immunotherapy N-Glycolyl Neuraminic Acid Nephroblastoma |
title_short |
Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry |
title_full |
Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry |
title_fullStr |
Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry |
title_full_unstemmed |
Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry |
title_sort |
Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry |
dc.creator.none.fl_str_mv |
Scursoni, Alejandra M. Galluzzo, Laura Camarero, Sandra Pozzo, Norma Gabri, Mariano Rolando De Acosta, Cristina Mateo Vázquez, Ana María Alonso, Daniel Fernando De Davila, María Teresa G. |
author |
Scursoni, Alejandra M. |
author_facet |
Scursoni, Alejandra M. Galluzzo, Laura Camarero, Sandra Pozzo, Norma Gabri, Mariano Rolando De Acosta, Cristina Mateo Vázquez, Ana María Alonso, Daniel Fernando De Davila, María Teresa G. |
author_role |
author |
author2 |
Galluzzo, Laura Camarero, Sandra Pozzo, Norma Gabri, Mariano Rolando De Acosta, Cristina Mateo Vázquez, Ana María Alonso, Daniel Fernando De Davila, María Teresa G. |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Gangliosides Gm3 Immunohistochemistry Immunotherapy N-Glycolyl Neuraminic Acid Nephroblastoma |
topic |
Gangliosides Gm3 Immunohistochemistry Immunotherapy N-Glycolyl Neuraminic Acid Nephroblastoma |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Gangliosides are glycolipids present on the cell surface. The N-glycolylated ganglioside NeuGc-GM3 has been described in some neoplasms, such as breast carcinoma and melanoma, but is usually not detected in normal human cells. Our aim was to evaluate the presence of NeuGc-GM3 in Wilms tumor by immunohistochemistry. Postchemotherapy tumors were grouped into different histologic subtypes considering the main preserved component. Formalin-fixed, paraffin-embedded tumor samples were cut into 5-mm sections. The monoclonal antibody 14F7, a mouse IgG1 that specifically recognizes NeuGc-GM3, and a peroxidase-labeled polymer conjugated to secondary antibodies were used. Sections from breast carcinoma were employed as positive controls. Presence of NeuGc-GM3 was evident in 22 of 25 (88%) cases. The staining was stronger in the epithelial component, with a membrane pattern and cytoplasmic diffusion. The stromal component expressed cytoplasmic NeuGc-GM3 in cells with rhabdomyoblastic differentiation. Tubules of adjacent renal tissue were also positive, but no expression of NeuGc-GM3 was detected in nontumoral fetal kidney. Until now, the expression of N-glycolylated gangliosides in pediatric solid tumors has not been investigated. The present study evidenced the expression of NeuGc-GM3 in a high proportion of Wilms tumors, suggesting its potential utility as a specific target of immunotherapy. Fil: Scursoni, Alejandra M.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina Fil: Galluzzo, Laura. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina Fil: Camarero, Sandra. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina Fil: Pozzo, Norma. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina Fil: Gabri, Mariano Rolando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: De Acosta, Cristina Mateo. Centro de Inmunología Molecular; Cuba Fil: Vázquez, Ana María. Centro de Inmunología Molecular; Cuba Fil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: De Davila, María Teresa G.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina |
description |
Gangliosides are glycolipids present on the cell surface. The N-glycolylated ganglioside NeuGc-GM3 has been described in some neoplasms, such as breast carcinoma and melanoma, but is usually not detected in normal human cells. Our aim was to evaluate the presence of NeuGc-GM3 in Wilms tumor by immunohistochemistry. Postchemotherapy tumors were grouped into different histologic subtypes considering the main preserved component. Formalin-fixed, paraffin-embedded tumor samples were cut into 5-mm sections. The monoclonal antibody 14F7, a mouse IgG1 that specifically recognizes NeuGc-GM3, and a peroxidase-labeled polymer conjugated to secondary antibodies were used. Sections from breast carcinoma were employed as positive controls. Presence of NeuGc-GM3 was evident in 22 of 25 (88%) cases. The staining was stronger in the epithelial component, with a membrane pattern and cytoplasmic diffusion. The stromal component expressed cytoplasmic NeuGc-GM3 in cells with rhabdomyoblastic differentiation. Tubules of adjacent renal tissue were also positive, but no expression of NeuGc-GM3 was detected in nontumoral fetal kidney. Until now, the expression of N-glycolylated gangliosides in pediatric solid tumors has not been investigated. The present study evidenced the expression of NeuGc-GM3 in a high proportion of Wilms tumors, suggesting its potential utility as a specific target of immunotherapy. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/54525 Scursoni, Alejandra M.; Galluzzo, Laura; Camarero, Sandra; Pozzo, Norma; Gabri, Mariano Rolando; et al.; Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry; Alliance Communications Group Division Allen Press; Pediatric And Developmental Pathology; 13; 1; 1-2010; 18-23 1093-5266 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/54525 |
identifier_str_mv |
Scursoni, Alejandra M.; Galluzzo, Laura; Camarero, Sandra; Pozzo, Norma; Gabri, Mariano Rolando; et al.; Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry; Alliance Communications Group Division Allen Press; Pediatric And Developmental Pathology; 13; 1; 1-2010; 18-23 1093-5266 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/abs/10.2350/08-10-0544.1 info:eu-repo/semantics/altIdentifier/doi/10.2350/08-10-0544.1 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Alliance Communications Group Division Allen Press |
publisher.none.fl_str_mv |
Alliance Communications Group Division Allen Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842268848625025024 |
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13.13397 |