Antitumor Protection by NGcGM3/VSSP Vaccine Against Transfected B16 Mouse Melanona Cells Overexpressing N-Glycolylated gangliosides

Autores
Segatori, Valeria Inés; Otero, Laura; Fernandez, Luis Enrique; Gomez, Daniel Eduardo; Alonso, Daniel Fernando; Gabri, Mariano Rolando
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Cancer vaccines are designed to modulate immunological responses against tumor cells through the presentation of tumor antigens. Materials and Methods: The mouse mRNA of the cytidine monophospho-N-acetylneuraminic acid hydroxylase (Cmah) gene, the enzyme that catalyzes the synthesis of N-glycolylneuraminic acid (NGc), was cloned and transfected into the B16 melanoma cell line. Transfected cells (B16-H) were characterized and used as an NGcGM3-positive primary tumor model for the evaluation of the therapeutic activity of the NGcGM3/VSSP vaccine. Results: The presence of NGcGM3 in B16-H cells promoted proliferation and adhesion in vitro, but resulted in reduced tumorigenicity in vivo. However, B16-H cells developed growing tumors in mice where NGcGM3/VSSP vaccination induced a therapeutic antitumor activity. NGcGM3/VSSP was ineffective in mice inoculated with parental B16 or B16-H cells that had lost NGcGM3 expression. Conclusion: The presence of NGcGM3 in tumor cells is critical for the antitumor activity of NGcGM3/VSSP vaccine.
Fil: Segatori, Valeria Inés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Otero, Laura. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez, Luis Enrique. Instituto de Inmunología Molecular; Cuba
Fil: Gomez, Daniel Eduardo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gabri, Mariano Rolando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
SIALIC ACID
N-GLYCOLYLATED GANGLIOSIDES
CYTIDINE MONOPHOSPHO-N-ACETYLNEURAMINIC HYDROXYLASE
CANCER
IMMUNOTHERAPY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195190
Acceder
id CONICETDig_3d76eeddb7de60939a21666bdced30f7
oai_identifier_str oai:ri.conicet.gov.ar:11336/195190
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antitumor Protection by NGcGM3/VSSP Vaccine Against Transfected B16 Mouse Melanona Cells Overexpressing N-Glycolylated gangliosidesSegatori, Valeria InésOtero, LauraFernandez, Luis EnriqueGomez, Daniel EduardoAlonso, Daniel FernandoGabri, Mariano RolandoSIALIC ACIDN-GLYCOLYLATED GANGLIOSIDESCYTIDINE MONOPHOSPHO-N-ACETYLNEURAMINIC HYDROXYLASECANCERIMMUNOTHERAPYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Cancer vaccines are designed to modulate immunological responses against tumor cells through the presentation of tumor antigens. Materials and Methods: The mouse mRNA of the cytidine monophospho-N-acetylneuraminic acid hydroxylase (Cmah) gene, the enzyme that catalyzes the synthesis of N-glycolylneuraminic acid (NGc), was cloned and transfected into the B16 melanoma cell line. Transfected cells (B16-H) were characterized and used as an NGcGM3-positive primary tumor model for the evaluation of the therapeutic activity of the NGcGM3/VSSP vaccine. Results: The presence of NGcGM3 in B16-H cells promoted proliferation and adhesion in vitro, but resulted in reduced tumorigenicity in vivo. However, B16-H cells developed growing tumors in mice where NGcGM3/VSSP vaccination induced a therapeutic antitumor activity. NGcGM3/VSSP was ineffective in mice inoculated with parental B16 or B16-H cells that had lost NGcGM3 expression. Conclusion: The presence of NGcGM3 in tumor cells is critical for the antitumor activity of NGcGM3/VSSP vaccine.Fil: Segatori, Valeria Inés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Otero, Laura. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernandez, Luis Enrique. Instituto de Inmunología Molecular; CubaFil: Gomez, Daniel Eduardo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gabri, Mariano Rolando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaInt Inst Anticancer Research2012-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195190Segatori, Valeria Inés; Otero, Laura; Fernandez, Luis Enrique; Gomez, Daniel Eduardo; Alonso, Daniel Fernando; et al.; Antitumor Protection by NGcGM3/VSSP Vaccine Against Transfected B16 Mouse Melanona Cells Overexpressing N-Glycolylated gangliosides; Int Inst Anticancer Research; In Vivo; 26; 4; 7-2012; 609-6170258-851X1791-7549CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://iv.iiarjournals.org/content/26/4/609/tab-figures-datainfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:44Zoai:ri.conicet.gov.ar:11336/195190instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:44.448CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antitumor Protection by NGcGM3/VSSP Vaccine Against Transfected B16 Mouse Melanona Cells Overexpressing N-Glycolylated gangliosides
title Antitumor Protection by NGcGM3/VSSP Vaccine Against Transfected B16 Mouse Melanona Cells Overexpressing N-Glycolylated gangliosides
spellingShingle Antitumor Protection by NGcGM3/VSSP Vaccine Against Transfected B16 Mouse Melanona Cells Overexpressing N-Glycolylated gangliosides
Segatori, Valeria Inés
SIALIC ACID
N-GLYCOLYLATED GANGLIOSIDES
CYTIDINE MONOPHOSPHO-N-ACETYLNEURAMINIC HYDROXYLASE
CANCER
IMMUNOTHERAPY
title_short Antitumor Protection by NGcGM3/VSSP Vaccine Against Transfected B16 Mouse Melanona Cells Overexpressing N-Glycolylated gangliosides
title_full Antitumor Protection by NGcGM3/VSSP Vaccine Against Transfected B16 Mouse Melanona Cells Overexpressing N-Glycolylated gangliosides
title_fullStr Antitumor Protection by NGcGM3/VSSP Vaccine Against Transfected B16 Mouse Melanona Cells Overexpressing N-Glycolylated gangliosides
title_full_unstemmed Antitumor Protection by NGcGM3/VSSP Vaccine Against Transfected B16 Mouse Melanona Cells Overexpressing N-Glycolylated gangliosides
title_sort Antitumor Protection by NGcGM3/VSSP Vaccine Against Transfected B16 Mouse Melanona Cells Overexpressing N-Glycolylated gangliosides
dc.creator.none.fl_str_mv Segatori, Valeria Inés
Otero, Laura
Fernandez, Luis Enrique
Gomez, Daniel Eduardo
Alonso, Daniel Fernando
Gabri, Mariano Rolando
author Segatori, Valeria Inés
author_facet Segatori, Valeria Inés
Otero, Laura
Fernandez, Luis Enrique
Gomez, Daniel Eduardo
Alonso, Daniel Fernando
Gabri, Mariano Rolando
author_role author
author2 Otero, Laura
Fernandez, Luis Enrique
Gomez, Daniel Eduardo
Alonso, Daniel Fernando
Gabri, Mariano Rolando
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv SIALIC ACID
N-GLYCOLYLATED GANGLIOSIDES
CYTIDINE MONOPHOSPHO-N-ACETYLNEURAMINIC HYDROXYLASE
CANCER
IMMUNOTHERAPY
topic SIALIC ACID
N-GLYCOLYLATED GANGLIOSIDES
CYTIDINE MONOPHOSPHO-N-ACETYLNEURAMINIC HYDROXYLASE
CANCER
IMMUNOTHERAPY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Cancer vaccines are designed to modulate immunological responses against tumor cells through the presentation of tumor antigens. Materials and Methods: The mouse mRNA of the cytidine monophospho-N-acetylneuraminic acid hydroxylase (Cmah) gene, the enzyme that catalyzes the synthesis of N-glycolylneuraminic acid (NGc), was cloned and transfected into the B16 melanoma cell line. Transfected cells (B16-H) were characterized and used as an NGcGM3-positive primary tumor model for the evaluation of the therapeutic activity of the NGcGM3/VSSP vaccine. Results: The presence of NGcGM3 in B16-H cells promoted proliferation and adhesion in vitro, but resulted in reduced tumorigenicity in vivo. However, B16-H cells developed growing tumors in mice where NGcGM3/VSSP vaccination induced a therapeutic antitumor activity. NGcGM3/VSSP was ineffective in mice inoculated with parental B16 or B16-H cells that had lost NGcGM3 expression. Conclusion: The presence of NGcGM3 in tumor cells is critical for the antitumor activity of NGcGM3/VSSP vaccine.
Fil: Segatori, Valeria Inés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Otero, Laura. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez, Luis Enrique. Instituto de Inmunología Molecular; Cuba
Fil: Gomez, Daniel Eduardo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gabri, Mariano Rolando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Background: Cancer vaccines are designed to modulate immunological responses against tumor cells through the presentation of tumor antigens. Materials and Methods: The mouse mRNA of the cytidine monophospho-N-acetylneuraminic acid hydroxylase (Cmah) gene, the enzyme that catalyzes the synthesis of N-glycolylneuraminic acid (NGc), was cloned and transfected into the B16 melanoma cell line. Transfected cells (B16-H) were characterized and used as an NGcGM3-positive primary tumor model for the evaluation of the therapeutic activity of the NGcGM3/VSSP vaccine. Results: The presence of NGcGM3 in B16-H cells promoted proliferation and adhesion in vitro, but resulted in reduced tumorigenicity in vivo. However, B16-H cells developed growing tumors in mice where NGcGM3/VSSP vaccination induced a therapeutic antitumor activity. NGcGM3/VSSP was ineffective in mice inoculated with parental B16 or B16-H cells that had lost NGcGM3 expression. Conclusion: The presence of NGcGM3 in tumor cells is critical for the antitumor activity of NGcGM3/VSSP vaccine.
publishDate 2012
dc.date.none.fl_str_mv 2012-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195190
Segatori, Valeria Inés; Otero, Laura; Fernandez, Luis Enrique; Gomez, Daniel Eduardo; Alonso, Daniel Fernando; et al.; Antitumor Protection by NGcGM3/VSSP Vaccine Against Transfected B16 Mouse Melanona Cells Overexpressing N-Glycolylated gangliosides; Int Inst Anticancer Research; In Vivo; 26; 4; 7-2012; 609-617
0258-851X
1791-7549
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195190
identifier_str_mv Segatori, Valeria Inés; Otero, Laura; Fernandez, Luis Enrique; Gomez, Daniel Eduardo; Alonso, Daniel Fernando; et al.; Antitumor Protection by NGcGM3/VSSP Vaccine Against Transfected B16 Mouse Melanona Cells Overexpressing N-Glycolylated gangliosides; Int Inst Anticancer Research; In Vivo; 26; 4; 7-2012; 609-617
0258-851X
1791-7549
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://iv.iiarjournals.org/content/26/4/609/tab-figures-data
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Int Inst Anticancer Research
publisher.none.fl_str_mv Int Inst Anticancer Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269244182495232
score 13.13397

Publicaciones similares