Pediococcus argentinicus sp. nov. from Argentinean fermented wheat flour and identification of Pediococcus species by pheS, rpoA and atpA sequence analysis

Autores
De Bruyne, Katrien; Franz, Charles M. A. P.; Vancanneyt, Marc; Schillinger, Ulrich; Mozzi, Fernanda Beatriz; Font, Graciela Maria; De Vuyst, Luc; Vandamme, Peter
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A Gram-positive, small coccus-shaped lactic acid bacterium, strain LMG 23999T, was isolated from Argentinean wheat flour. 16S rRNA gene sequence analysis revealed that the phylogenetic position of the novel strain was within the genus Pediococcus, with Pediococcus stilesii, Pediococcus pentosaceus and Pediococcus acidilactici as its closest relatives (97.7, 97.3 and 96.9% gene sequence similarity, respectively). Fluorescent amplified fragment length polymorphism fingerprinting of whole genomes and whole-cell protein electrophoresis confirmed the unique taxonomic status of the novel strain. DNA-DNA hybridizations, DNA G+C content determination, comparative sequence analysis of the pheS, rpoA and atpA genes and physiological and biochemical characterization demonstrated that strain LMG 23999T (=CCUG 54535T=CRL 776T) represents a novel species for which the name Pediococcus argentinicus sp. nov. is proposed. Multi-locus sequence analysis based on pheS, rpoA and atpA genes was found to be a suitable method for the identification of species of the genus Pediococcus.
Fil: De Bruyne, Katrien. Ghent University; Bélgica
Fil: Franz, Charles M. A. P.. Institute of Hygiene and Toxicology. Federal Research Centre for Nutrition and Food; Alemania
Fil: Vancanneyt, Marc. Ghent University; Bélgica
Fil: Schillinger, Ulrich. Institute of Hygiene and Toxicology. Federal Research Centre for Nutrition and Food; Alemania
Fil: Mozzi, Fernanda Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: De Vuyst, Luc. Vrije Universiteit Brussel; Bélgica
Fil: Vandamme, Peter. Ghent University; Bélgica
Materia
Pediococcus
Fermented Wheat Flour
Identification
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/58812
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/58812
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Pediococcus argentinicus sp. nov. from Argentinean fermented wheat flour and identification of Pediococcus species by pheS, rpoA and atpA sequence analysisDe Bruyne, KatrienFranz, Charles M. A. P.Vancanneyt, MarcSchillinger, UlrichMozzi, Fernanda BeatrizFont, Graciela MariaDe Vuyst, LucVandamme, PeterPediococcusFermented Wheat FlourIdentificationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A Gram-positive, small coccus-shaped lactic acid bacterium, strain LMG 23999T, was isolated from Argentinean wheat flour. 16S rRNA gene sequence analysis revealed that the phylogenetic position of the novel strain was within the genus Pediococcus, with Pediococcus stilesii, Pediococcus pentosaceus and Pediococcus acidilactici as its closest relatives (97.7, 97.3 and 96.9% gene sequence similarity, respectively). Fluorescent amplified fragment length polymorphism fingerprinting of whole genomes and whole-cell protein electrophoresis confirmed the unique taxonomic status of the novel strain. DNA-DNA hybridizations, DNA G+C content determination, comparative sequence analysis of the pheS, rpoA and atpA genes and physiological and biochemical characterization demonstrated that strain LMG 23999T (=CCUG 54535T=CRL 776T) represents a novel species for which the name Pediococcus argentinicus sp. nov. is proposed. Multi-locus sequence analysis based on pheS, rpoA and atpA genes was found to be a suitable method for the identification of species of the genus Pediococcus.Fil: De Bruyne, Katrien. Ghent University; BélgicaFil: Franz, Charles M. A. P.. Institute of Hygiene and Toxicology. Federal Research Centre for Nutrition and Food; AlemaniaFil: Vancanneyt, Marc. Ghent University; BélgicaFil: Schillinger, Ulrich. Institute of Hygiene and Toxicology. Federal Research Centre for Nutrition and Food; AlemaniaFil: Mozzi, Fernanda Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: De Vuyst, Luc. Vrije Universiteit Brussel; BélgicaFil: Vandamme, Peter. Ghent University; BélgicaSociety for General Microbiology2008-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/58812De Bruyne, Katrien; Franz, Charles M. A. P.; Vancanneyt, Marc; Schillinger, Ulrich; Mozzi, Fernanda Beatriz; et al.; Pediococcus argentinicus sp. nov. from Argentinean fermented wheat flour and identification of Pediococcus species by pheS, rpoA and atpA sequence analysis; Society for General Microbiology; International Journal of Systematic and Evolutionary Microbiology; 58; 12; 1-12-2008; 2909-29161466-50261466-5034CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1099/ijs.0.65833-0info:eu-repo/semantics/altIdentifier/url/http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65833-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:58Zoai:ri.conicet.gov.ar:11336/58812instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:58.889CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Pediococcus argentinicus sp. nov. from Argentinean fermented wheat flour and identification of Pediococcus species by pheS, rpoA and atpA sequence analysis
title Pediococcus argentinicus sp. nov. from Argentinean fermented wheat flour and identification of Pediococcus species by pheS, rpoA and atpA sequence analysis
spellingShingle Pediococcus argentinicus sp. nov. from Argentinean fermented wheat flour and identification of Pediococcus species by pheS, rpoA and atpA sequence analysis
De Bruyne, Katrien
Pediococcus
Fermented Wheat Flour
Identification
title_short Pediococcus argentinicus sp. nov. from Argentinean fermented wheat flour and identification of Pediococcus species by pheS, rpoA and atpA sequence analysis
title_full Pediococcus argentinicus sp. nov. from Argentinean fermented wheat flour and identification of Pediococcus species by pheS, rpoA and atpA sequence analysis
title_fullStr Pediococcus argentinicus sp. nov. from Argentinean fermented wheat flour and identification of Pediococcus species by pheS, rpoA and atpA sequence analysis
title_full_unstemmed Pediococcus argentinicus sp. nov. from Argentinean fermented wheat flour and identification of Pediococcus species by pheS, rpoA and atpA sequence analysis
title_sort Pediococcus argentinicus sp. nov. from Argentinean fermented wheat flour and identification of Pediococcus species by pheS, rpoA and atpA sequence analysis
dc.creator.none.fl_str_mv De Bruyne, Katrien
Franz, Charles M. A. P.
Vancanneyt, Marc
Schillinger, Ulrich
Mozzi, Fernanda Beatriz
Font, Graciela Maria
De Vuyst, Luc
Vandamme, Peter
author De Bruyne, Katrien
author_facet De Bruyne, Katrien
Franz, Charles M. A. P.
Vancanneyt, Marc
Schillinger, Ulrich
Mozzi, Fernanda Beatriz
Font, Graciela Maria
De Vuyst, Luc
Vandamme, Peter
author_role author
author2 Franz, Charles M. A. P.
Vancanneyt, Marc
Schillinger, Ulrich
Mozzi, Fernanda Beatriz
Font, Graciela Maria
De Vuyst, Luc
Vandamme, Peter
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Pediococcus
Fermented Wheat Flour
Identification
topic Pediococcus
Fermented Wheat Flour
Identification
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A Gram-positive, small coccus-shaped lactic acid bacterium, strain LMG 23999T, was isolated from Argentinean wheat flour. 16S rRNA gene sequence analysis revealed that the phylogenetic position of the novel strain was within the genus Pediococcus, with Pediococcus stilesii, Pediococcus pentosaceus and Pediococcus acidilactici as its closest relatives (97.7, 97.3 and 96.9% gene sequence similarity, respectively). Fluorescent amplified fragment length polymorphism fingerprinting of whole genomes and whole-cell protein electrophoresis confirmed the unique taxonomic status of the novel strain. DNA-DNA hybridizations, DNA G+C content determination, comparative sequence analysis of the pheS, rpoA and atpA genes and physiological and biochemical characterization demonstrated that strain LMG 23999T (=CCUG 54535T=CRL 776T) represents a novel species for which the name Pediococcus argentinicus sp. nov. is proposed. Multi-locus sequence analysis based on pheS, rpoA and atpA genes was found to be a suitable method for the identification of species of the genus Pediococcus.
Fil: De Bruyne, Katrien. Ghent University; Bélgica
Fil: Franz, Charles M. A. P.. Institute of Hygiene and Toxicology. Federal Research Centre for Nutrition and Food; Alemania
Fil: Vancanneyt, Marc. Ghent University; Bélgica
Fil: Schillinger, Ulrich. Institute of Hygiene and Toxicology. Federal Research Centre for Nutrition and Food; Alemania
Fil: Mozzi, Fernanda Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: De Vuyst, Luc. Vrije Universiteit Brussel; Bélgica
Fil: Vandamme, Peter. Ghent University; Bélgica
description A Gram-positive, small coccus-shaped lactic acid bacterium, strain LMG 23999T, was isolated from Argentinean wheat flour. 16S rRNA gene sequence analysis revealed that the phylogenetic position of the novel strain was within the genus Pediococcus, with Pediococcus stilesii, Pediococcus pentosaceus and Pediococcus acidilactici as its closest relatives (97.7, 97.3 and 96.9% gene sequence similarity, respectively). Fluorescent amplified fragment length polymorphism fingerprinting of whole genomes and whole-cell protein electrophoresis confirmed the unique taxonomic status of the novel strain. DNA-DNA hybridizations, DNA G+C content determination, comparative sequence analysis of the pheS, rpoA and atpA genes and physiological and biochemical characterization demonstrated that strain LMG 23999T (=CCUG 54535T=CRL 776T) represents a novel species for which the name Pediococcus argentinicus sp. nov. is proposed. Multi-locus sequence analysis based on pheS, rpoA and atpA genes was found to be a suitable method for the identification of species of the genus Pediococcus.
publishDate 2008
dc.date.none.fl_str_mv 2008-12-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/58812
De Bruyne, Katrien; Franz, Charles M. A. P.; Vancanneyt, Marc; Schillinger, Ulrich; Mozzi, Fernanda Beatriz; et al.; Pediococcus argentinicus sp. nov. from Argentinean fermented wheat flour and identification of Pediococcus species by pheS, rpoA and atpA sequence analysis; Society for General Microbiology; International Journal of Systematic and Evolutionary Microbiology; 58; 12; 1-12-2008; 2909-2916
1466-5026
1466-5034
CONICET Digital
CONICET
url http://hdl.handle.net/11336/58812
identifier_str_mv De Bruyne, Katrien; Franz, Charles M. A. P.; Vancanneyt, Marc; Schillinger, Ulrich; Mozzi, Fernanda Beatriz; et al.; Pediococcus argentinicus sp. nov. from Argentinean fermented wheat flour and identification of Pediococcus species by pheS, rpoA and atpA sequence analysis; Society for General Microbiology; International Journal of Systematic and Evolutionary Microbiology; 58; 12; 1-12-2008; 2909-2916
1466-5026
1466-5034
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1099/ijs.0.65833-0
info:eu-repo/semantics/altIdentifier/url/http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65833-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for General Microbiology
publisher.none.fl_str_mv Society for General Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269431949950976
score 13.13397

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