Purification and biochemical characterization of IMP-13 metallo-β-lactamase

Autores
Santella, Gisela; Docquier, Jean Denis; Gutkind, Gabriel Osvaldo; Rossolini, Gian Maria; Radice, Marcela Alejandra
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The IMP-13 metallo-β-lactamase was overproduced in Escherichia coli BL21(DE3) and purified by chromatography. Analysis of kinetic parameters revealed some notable differences with other IMP-type enzymes, noteworthily a higher catalytic efficiency toward ticarcillin and piperacillin and a marked preference for imipenem over meropenem.
Fil: Santella, Gisela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Docquier, Jean Denis. Universita Degli Studi Di Siena; Italia
Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Rossolini, Gian Maria. Universita Degli Studi Di Siena; Italia
Fil: Radice, Marcela Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Materia
Metallo-Β-Lactamase
Carbapenem Resistance
Mbl Purification
Enzyme Kinetic
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13308
Acceder
id CONICETDig_7a8eeb0b3c00165f62e179153631b389
oai_identifier_str oai:ri.conicet.gov.ar:11336/13308
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Purification and biochemical characterization of IMP-13 metallo-β-lactamaseSantella, GiselaDocquier, Jean DenisGutkind, Gabriel OsvaldoRossolini, Gian MariaRadice, Marcela AlejandraMetallo-Β-LactamaseCarbapenem ResistanceMbl PurificationEnzyme Kinetichttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The IMP-13 metallo-β-lactamase was overproduced in Escherichia coli BL21(DE3) and purified by chromatography. Analysis of kinetic parameters revealed some notable differences with other IMP-type enzymes, noteworthily a higher catalytic efficiency toward ticarcillin and piperacillin and a marked preference for imipenem over meropenem.Fil: Santella, Gisela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Docquier, Jean Denis. Universita Degli Studi Di Siena; ItaliaFil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Rossolini, Gian Maria. Universita Degli Studi Di Siena; ItaliaFil: Radice, Marcela Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaAmerican Society For Microbiology2011-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13308Santella, Gisela; Docquier, Jean Denis; Gutkind, Gabriel Osvaldo; Rossolini, Gian Maria; Radice, Marcela Alejandra; Purification and biochemical characterization of IMP-13 metallo-β-lactamase; American Society For Microbiology; Antimicrobial Agents And Chemotherapy; 55; 1; 1-2011; 399-4010066-4804enginfo:eu-repo/semantics/altIdentifier/url/http://aac.asm.org/content/55/1/399info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1128/AAC.00421-10info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:27:19Zoai:ri.conicet.gov.ar:11336/13308instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:27:19.74CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Purification and biochemical characterization of IMP-13 metallo-β-lactamase
title Purification and biochemical characterization of IMP-13 metallo-β-lactamase
spellingShingle Purification and biochemical characterization of IMP-13 metallo-β-lactamase
Santella, Gisela
Metallo-Β-Lactamase
Carbapenem Resistance
Mbl Purification
Enzyme Kinetic
title_short Purification and biochemical characterization of IMP-13 metallo-β-lactamase
title_full Purification and biochemical characterization of IMP-13 metallo-β-lactamase
title_fullStr Purification and biochemical characterization of IMP-13 metallo-β-lactamase
title_full_unstemmed Purification and biochemical characterization of IMP-13 metallo-β-lactamase
title_sort Purification and biochemical characterization of IMP-13 metallo-β-lactamase
dc.creator.none.fl_str_mv Santella, Gisela
Docquier, Jean Denis
Gutkind, Gabriel Osvaldo
Rossolini, Gian Maria
Radice, Marcela Alejandra
author Santella, Gisela
author_facet Santella, Gisela
Docquier, Jean Denis
Gutkind, Gabriel Osvaldo
Rossolini, Gian Maria
Radice, Marcela Alejandra
author_role author
author2 Docquier, Jean Denis
Gutkind, Gabriel Osvaldo
Rossolini, Gian Maria
Radice, Marcela Alejandra
author2_role author
author
author
author
dc.subject.none.fl_str_mv Metallo-Β-Lactamase
Carbapenem Resistance
Mbl Purification
Enzyme Kinetic
topic Metallo-Β-Lactamase
Carbapenem Resistance
Mbl Purification
Enzyme Kinetic
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The IMP-13 metallo-β-lactamase was overproduced in Escherichia coli BL21(DE3) and purified by chromatography. Analysis of kinetic parameters revealed some notable differences with other IMP-type enzymes, noteworthily a higher catalytic efficiency toward ticarcillin and piperacillin and a marked preference for imipenem over meropenem.
Fil: Santella, Gisela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Docquier, Jean Denis. Universita Degli Studi Di Siena; Italia
Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Rossolini, Gian Maria. Universita Degli Studi Di Siena; Italia
Fil: Radice, Marcela Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
description The IMP-13 metallo-β-lactamase was overproduced in Escherichia coli BL21(DE3) and purified by chromatography. Analysis of kinetic parameters revealed some notable differences with other IMP-type enzymes, noteworthily a higher catalytic efficiency toward ticarcillin and piperacillin and a marked preference for imipenem over meropenem.
publishDate 2011
dc.date.none.fl_str_mv 2011-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13308
Santella, Gisela; Docquier, Jean Denis; Gutkind, Gabriel Osvaldo; Rossolini, Gian Maria; Radice, Marcela Alejandra; Purification and biochemical characterization of IMP-13 metallo-β-lactamase; American Society For Microbiology; Antimicrobial Agents And Chemotherapy; 55; 1; 1-2011; 399-401
0066-4804
url http://hdl.handle.net/11336/13308
identifier_str_mv Santella, Gisela; Docquier, Jean Denis; Gutkind, Gabriel Osvaldo; Rossolini, Gian Maria; Radice, Marcela Alejandra; Purification and biochemical characterization of IMP-13 metallo-β-lactamase; American Society For Microbiology; Antimicrobial Agents And Chemotherapy; 55; 1; 1-2011; 399-401
0066-4804
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://aac.asm.org/content/55/1/399
info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1128/AAC.00421-10
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society For Microbiology
publisher.none.fl_str_mv American Society For Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781840102260736
score 12.982451

Publicaciones similares