The Protective Effect of N-Acetylcysteine on Oxidative Stress in the Brain Caused by the Long-Term Intake of Aspartame by Rats

Autores
Finamor, Isabela A.; Ourique, Giovana M.; Pês, Tanise S.; Saccol, Etiane M. H.; Bressan, Caroline A.; Scheid, Taína; Baldisserotto, Bernardo; Llesuy, Susana Francisca; Partata, Wânia A.; Pavanato, Maria A.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Long-term intake of aspartame at the acceptable daily dose causes oxidative stress in rodent brain mainly due to the dysregulation of glutathione (GSH) homeostasis. N-Acetylcysteine provides the cysteine that is required for the production of GSH, being effective in treating disorders associated with oxidative stress. We investigated the effects of N-acetylcysteine treatment (150 mg kg(-1), i.p.) on oxidative stress biomarkers in rat brain after chronic aspartame administration by gavage (40 mg kg(-1)). N-Acetylcysteine led to a reduction in the thiobarbituric acid reactive substances, lipid hydroperoxides, and carbonyl protein levels, which were increased due to aspartame administration. N-Acetylcysteine also resulted in an elevation of superoxide dismutase, glutathione peroxidase, glutathione reductase activities, as well as non-protein thiols, and total reactive antioxidant potential levels, which were decreased after aspartame exposure. However, N-acetylcysteine was unable to reduce serum glucose levels, which were increased as a result of aspartame administration. Furthermore, catalase and glutathione S-transferase, whose activities were reduced due to aspartame treatment, remained decreased even after N-acetylcysteine exposure. In conclusion, N-acetylcysteine treatment may exert a protective effect against the oxidative damage in the brain, which was caused by the long-term consumption of the acceptable daily dose of aspartame by rats.
Fil: Finamor, Isabela A.. Universidade Federal de Santa Maria; Brasil
Fil: Ourique, Giovana M.. Universidade Federal de Santa Maria; Brasil
Fil: Pês, Tanise S.. Universidade Federal de Santa Maria; Brasil
Fil: Saccol, Etiane M. H.. Universidade Federal de Santa Maria; Brasil
Fil: Bressan, Caroline A.. Universidade Federal de Santa Maria; Brasil
Fil: Scheid, Taína. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Baldisserotto, Bernardo. Universidade Federal de Santa Maria; Brasil
Fil: Llesuy, Susana Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Partata, Wânia A.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Pavanato, Maria A.. Universidade Federal do Rio Grande do Sul; Brasil
Materia
N-Acetylcysteine Protective
Aspartame
Brain
Oxidative Damage
Glutathione
Protective
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30773
Acceder
id CONICETDig_7a74023827d078a84485f47c23ec74dc
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The Protective Effect of N-Acetylcysteine on Oxidative Stress in the Brain Caused by the Long-Term Intake of Aspartame by RatsFinamor, Isabela A.Ourique, Giovana M.Pês, Tanise S.Saccol, Etiane M. H.Bressan, Caroline A.Scheid, TaínaBaldisserotto, BernardoLlesuy, Susana FranciscaPartata, Wânia A.Pavanato, Maria A.N-Acetylcysteine ProtectiveAspartameBrainOxidative DamageGlutathioneProtectivehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Long-term intake of aspartame at the acceptable daily dose causes oxidative stress in rodent brain mainly due to the dysregulation of glutathione (GSH) homeostasis. N-Acetylcysteine provides the cysteine that is required for the production of GSH, being effective in treating disorders associated with oxidative stress. We investigated the effects of N-acetylcysteine treatment (150 mg kg(-1), i.p.) on oxidative stress biomarkers in rat brain after chronic aspartame administration by gavage (40 mg kg(-1)). N-Acetylcysteine led to a reduction in the thiobarbituric acid reactive substances, lipid hydroperoxides, and carbonyl protein levels, which were increased due to aspartame administration. N-Acetylcysteine also resulted in an elevation of superoxide dismutase, glutathione peroxidase, glutathione reductase activities, as well as non-protein thiols, and total reactive antioxidant potential levels, which were decreased after aspartame exposure. However, N-acetylcysteine was unable to reduce serum glucose levels, which were increased as a result of aspartame administration. Furthermore, catalase and glutathione S-transferase, whose activities were reduced due to aspartame treatment, remained decreased even after N-acetylcysteine exposure. In conclusion, N-acetylcysteine treatment may exert a protective effect against the oxidative damage in the brain, which was caused by the long-term consumption of the acceptable daily dose of aspartame by rats.Fil: Finamor, Isabela A.. Universidade Federal de Santa Maria; BrasilFil: Ourique, Giovana M.. Universidade Federal de Santa Maria; BrasilFil: Pês, Tanise S.. Universidade Federal de Santa Maria; BrasilFil: Saccol, Etiane M. H.. Universidade Federal de Santa Maria; BrasilFil: Bressan, Caroline A.. Universidade Federal de Santa Maria; BrasilFil: Scheid, Taína. Universidade Federal do Rio Grande do Sul; BrasilFil: Baldisserotto, Bernardo. Universidade Federal de Santa Maria; BrasilFil: Llesuy, Susana Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Partata, Wânia A.. Universidade Federal do Rio Grande do Sul; BrasilFil: Pavanato, Maria A.. Universidade Federal do Rio Grande do Sul; BrasilSpringer2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30773Pavanato, Maria A.; Partata, Wânia A.; Llesuy, Susana Francisca; Baldisserotto, Bernardo; Scheid, Taína; Bressan, Caroline A.; et al.; The Protective Effect of N-Acetylcysteine on Oxidative Stress in the Brain Caused by the Long-Term Intake of Aspartame by Rats; Springer; Neurochemical Research; 39; 9; 9-2014; 1681-16900364-31901573-6903CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11064-014-1360-9info:eu-repo/semantics/altIdentifier/doi/10.1007/s11064-014-1360-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:31:07Zoai:ri.conicet.gov.ar:11336/30773instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:31:08.153CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The Protective Effect of N-Acetylcysteine on Oxidative Stress in the Brain Caused by the Long-Term Intake of Aspartame by Rats
title The Protective Effect of N-Acetylcysteine on Oxidative Stress in the Brain Caused by the Long-Term Intake of Aspartame by Rats
spellingShingle The Protective Effect of N-Acetylcysteine on Oxidative Stress in the Brain Caused by the Long-Term Intake of Aspartame by Rats
Finamor, Isabela A.
N-Acetylcysteine Protective
Aspartame
Brain
Oxidative Damage
Glutathione
Protective
title_short The Protective Effect of N-Acetylcysteine on Oxidative Stress in the Brain Caused by the Long-Term Intake of Aspartame by Rats
title_full The Protective Effect of N-Acetylcysteine on Oxidative Stress in the Brain Caused by the Long-Term Intake of Aspartame by Rats
title_fullStr The Protective Effect of N-Acetylcysteine on Oxidative Stress in the Brain Caused by the Long-Term Intake of Aspartame by Rats
title_full_unstemmed The Protective Effect of N-Acetylcysteine on Oxidative Stress in the Brain Caused by the Long-Term Intake of Aspartame by Rats
title_sort The Protective Effect of N-Acetylcysteine on Oxidative Stress in the Brain Caused by the Long-Term Intake of Aspartame by Rats
dc.creator.none.fl_str_mv Finamor, Isabela A.
Ourique, Giovana M.
Pês, Tanise S.
Saccol, Etiane M. H.
Bressan, Caroline A.
Scheid, Taína
Baldisserotto, Bernardo
Llesuy, Susana Francisca
Partata, Wânia A.
Pavanato, Maria A.
author Finamor, Isabela A.
author_facet Finamor, Isabela A.
Ourique, Giovana M.
Pês, Tanise S.
Saccol, Etiane M. H.
Bressan, Caroline A.
Scheid, Taína
Baldisserotto, Bernardo
Llesuy, Susana Francisca
Partata, Wânia A.
Pavanato, Maria A.
author_role author
author2 Ourique, Giovana M.
Pês, Tanise S.
Saccol, Etiane M. H.
Bressan, Caroline A.
Scheid, Taína
Baldisserotto, Bernardo
Llesuy, Susana Francisca
Partata, Wânia A.
Pavanato, Maria A.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv N-Acetylcysteine Protective
Aspartame
Brain
Oxidative Damage
Glutathione
Protective
topic N-Acetylcysteine Protective
Aspartame
Brain
Oxidative Damage
Glutathione
Protective
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Long-term intake of aspartame at the acceptable daily dose causes oxidative stress in rodent brain mainly due to the dysregulation of glutathione (GSH) homeostasis. N-Acetylcysteine provides the cysteine that is required for the production of GSH, being effective in treating disorders associated with oxidative stress. We investigated the effects of N-acetylcysteine treatment (150 mg kg(-1), i.p.) on oxidative stress biomarkers in rat brain after chronic aspartame administration by gavage (40 mg kg(-1)). N-Acetylcysteine led to a reduction in the thiobarbituric acid reactive substances, lipid hydroperoxides, and carbonyl protein levels, which were increased due to aspartame administration. N-Acetylcysteine also resulted in an elevation of superoxide dismutase, glutathione peroxidase, glutathione reductase activities, as well as non-protein thiols, and total reactive antioxidant potential levels, which were decreased after aspartame exposure. However, N-acetylcysteine was unable to reduce serum glucose levels, which were increased as a result of aspartame administration. Furthermore, catalase and glutathione S-transferase, whose activities were reduced due to aspartame treatment, remained decreased even after N-acetylcysteine exposure. In conclusion, N-acetylcysteine treatment may exert a protective effect against the oxidative damage in the brain, which was caused by the long-term consumption of the acceptable daily dose of aspartame by rats.
Fil: Finamor, Isabela A.. Universidade Federal de Santa Maria; Brasil
Fil: Ourique, Giovana M.. Universidade Federal de Santa Maria; Brasil
Fil: Pês, Tanise S.. Universidade Federal de Santa Maria; Brasil
Fil: Saccol, Etiane M. H.. Universidade Federal de Santa Maria; Brasil
Fil: Bressan, Caroline A.. Universidade Federal de Santa Maria; Brasil
Fil: Scheid, Taína. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Baldisserotto, Bernardo. Universidade Federal de Santa Maria; Brasil
Fil: Llesuy, Susana Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Partata, Wânia A.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Pavanato, Maria A.. Universidade Federal do Rio Grande do Sul; Brasil
description Long-term intake of aspartame at the acceptable daily dose causes oxidative stress in rodent brain mainly due to the dysregulation of glutathione (GSH) homeostasis. N-Acetylcysteine provides the cysteine that is required for the production of GSH, being effective in treating disorders associated with oxidative stress. We investigated the effects of N-acetylcysteine treatment (150 mg kg(-1), i.p.) on oxidative stress biomarkers in rat brain after chronic aspartame administration by gavage (40 mg kg(-1)). N-Acetylcysteine led to a reduction in the thiobarbituric acid reactive substances, lipid hydroperoxides, and carbonyl protein levels, which were increased due to aspartame administration. N-Acetylcysteine also resulted in an elevation of superoxide dismutase, glutathione peroxidase, glutathione reductase activities, as well as non-protein thiols, and total reactive antioxidant potential levels, which were decreased after aspartame exposure. However, N-acetylcysteine was unable to reduce serum glucose levels, which were increased as a result of aspartame administration. Furthermore, catalase and glutathione S-transferase, whose activities were reduced due to aspartame treatment, remained decreased even after N-acetylcysteine exposure. In conclusion, N-acetylcysteine treatment may exert a protective effect against the oxidative damage in the brain, which was caused by the long-term consumption of the acceptable daily dose of aspartame by rats.
publishDate 2014
dc.date.none.fl_str_mv 2014-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30773
Pavanato, Maria A.; Partata, Wânia A.; Llesuy, Susana Francisca; Baldisserotto, Bernardo; Scheid, Taína; Bressan, Caroline A.; et al.; The Protective Effect of N-Acetylcysteine on Oxidative Stress in the Brain Caused by the Long-Term Intake of Aspartame by Rats; Springer; Neurochemical Research; 39; 9; 9-2014; 1681-1690
0364-3190
1573-6903
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30773
identifier_str_mv Pavanato, Maria A.; Partata, Wânia A.; Llesuy, Susana Francisca; Baldisserotto, Bernardo; Scheid, Taína; Bressan, Caroline A.; et al.; The Protective Effect of N-Acetylcysteine on Oxidative Stress in the Brain Caused by the Long-Term Intake of Aspartame by Rats; Springer; Neurochemical Research; 39; 9; 9-2014; 1681-1690
0364-3190
1573-6903
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11064-014-1360-9
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11064-014-1360-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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