Cytotoxic effect of potato aspartic proteases (StAPs) on Jurkat T cells

Autores
Mendieta, Julieta Renee; Figmonari, Carmela; Daleo, Gustavo Raul; Hrelia, Patrizia; Guevara, Maria Gabriela
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
StAPs are potato aspartic proteases with cytotoxic activity against plant pathogens and spermatozoa. StAPs cytotoxic activity is selective, since these proteins do not exert toxic effect on plant cells and erythrocytes. In this work, we investigated the capacity of StAPs to exert cytotoxicity on human leukaemia cells. Obtained results show that StAPs induce apoptosis on Jurkat T cells after a short time of incubation in a dose-dependent manner. However, no significative effect on the T lymphocytes viability was observed at all StAPs incubation times and concentrations tested. These results suggest that StAPs can be conceptually promising leads for cancer therapy.
Fil: Mendieta, Julieta Renee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Figmonari, Carmela. Universidad de Bologna; Italia
Fil: Daleo, Gustavo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Hrelia, Patrizia. Universidad de Bologna; Italia
Fil: Guevara, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Materia
ANTIMICROBIAL PLANT PROTEINS
APOPTOSIS
ASPARTIC PROTEASES
JURKAT T CELLS
NECROSIS
T LYMPHOCYTES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/138195
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cytotoxic effect of potato aspartic proteases (StAPs) on Jurkat T cellsMendieta, Julieta ReneeFigmonari, CarmelaDaleo, Gustavo RaulHrelia, PatriziaGuevara, Maria GabrielaANTIMICROBIAL PLANT PROTEINSAPOPTOSISASPARTIC PROTEASESJURKAT T CELLSNECROSIST LYMPHOCYTEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1StAPs are potato aspartic proteases with cytotoxic activity against plant pathogens and spermatozoa. StAPs cytotoxic activity is selective, since these proteins do not exert toxic effect on plant cells and erythrocytes. In this work, we investigated the capacity of StAPs to exert cytotoxicity on human leukaemia cells. Obtained results show that StAPs induce apoptosis on Jurkat T cells after a short time of incubation in a dose-dependent manner. However, no significative effect on the T lymphocytes viability was observed at all StAPs incubation times and concentrations tested. These results suggest that StAPs can be conceptually promising leads for cancer therapy.Fil: Mendieta, Julieta Renee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Figmonari, Carmela. Universidad de Bologna; ItaliaFil: Daleo, Gustavo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Hrelia, Patrizia. Universidad de Bologna; ItaliaFil: Guevara, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaElsevier Science2009-10-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/138195Mendieta, Julieta Renee; Figmonari, Carmela; Daleo, Gustavo Raul; Hrelia, Patrizia; Guevara, Maria Gabriela; Cytotoxic effect of potato aspartic proteases (StAPs) on Jurkat T cells; Elsevier Science; Fitoterapia; 81; 5; 13-10-2009; 329-3350367-326XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.fitote.2009.10.004info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0367326X09002494info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:27:09Zoai:ri.conicet.gov.ar:11336/138195instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:27:10.051CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cytotoxic effect of potato aspartic proteases (StAPs) on Jurkat T cells
title Cytotoxic effect of potato aspartic proteases (StAPs) on Jurkat T cells
spellingShingle Cytotoxic effect of potato aspartic proteases (StAPs) on Jurkat T cells
Mendieta, Julieta Renee
ANTIMICROBIAL PLANT PROTEINS
APOPTOSIS
ASPARTIC PROTEASES
JURKAT T CELLS
NECROSIS
T LYMPHOCYTES
title_short Cytotoxic effect of potato aspartic proteases (StAPs) on Jurkat T cells
title_full Cytotoxic effect of potato aspartic proteases (StAPs) on Jurkat T cells
title_fullStr Cytotoxic effect of potato aspartic proteases (StAPs) on Jurkat T cells
title_full_unstemmed Cytotoxic effect of potato aspartic proteases (StAPs) on Jurkat T cells
title_sort Cytotoxic effect of potato aspartic proteases (StAPs) on Jurkat T cells
dc.creator.none.fl_str_mv Mendieta, Julieta Renee
Figmonari, Carmela
Daleo, Gustavo Raul
Hrelia, Patrizia
Guevara, Maria Gabriela
author Mendieta, Julieta Renee
author_facet Mendieta, Julieta Renee
Figmonari, Carmela
Daleo, Gustavo Raul
Hrelia, Patrizia
Guevara, Maria Gabriela
author_role author
author2 Figmonari, Carmela
Daleo, Gustavo Raul
Hrelia, Patrizia
Guevara, Maria Gabriela
author2_role author
author
author
author
dc.subject.none.fl_str_mv ANTIMICROBIAL PLANT PROTEINS
APOPTOSIS
ASPARTIC PROTEASES
JURKAT T CELLS
NECROSIS
T LYMPHOCYTES
topic ANTIMICROBIAL PLANT PROTEINS
APOPTOSIS
ASPARTIC PROTEASES
JURKAT T CELLS
NECROSIS
T LYMPHOCYTES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv StAPs are potato aspartic proteases with cytotoxic activity against plant pathogens and spermatozoa. StAPs cytotoxic activity is selective, since these proteins do not exert toxic effect on plant cells and erythrocytes. In this work, we investigated the capacity of StAPs to exert cytotoxicity on human leukaemia cells. Obtained results show that StAPs induce apoptosis on Jurkat T cells after a short time of incubation in a dose-dependent manner. However, no significative effect on the T lymphocytes viability was observed at all StAPs incubation times and concentrations tested. These results suggest that StAPs can be conceptually promising leads for cancer therapy.
Fil: Mendieta, Julieta Renee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Figmonari, Carmela. Universidad de Bologna; Italia
Fil: Daleo, Gustavo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Hrelia, Patrizia. Universidad de Bologna; Italia
Fil: Guevara, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
description StAPs are potato aspartic proteases with cytotoxic activity against plant pathogens and spermatozoa. StAPs cytotoxic activity is selective, since these proteins do not exert toxic effect on plant cells and erythrocytes. In this work, we investigated the capacity of StAPs to exert cytotoxicity on human leukaemia cells. Obtained results show that StAPs induce apoptosis on Jurkat T cells after a short time of incubation in a dose-dependent manner. However, no significative effect on the T lymphocytes viability was observed at all StAPs incubation times and concentrations tested. These results suggest that StAPs can be conceptually promising leads for cancer therapy.
publishDate 2009
dc.date.none.fl_str_mv 2009-10-13
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/138195
Mendieta, Julieta Renee; Figmonari, Carmela; Daleo, Gustavo Raul; Hrelia, Patrizia; Guevara, Maria Gabriela; Cytotoxic effect of potato aspartic proteases (StAPs) on Jurkat T cells; Elsevier Science; Fitoterapia; 81; 5; 13-10-2009; 329-335
0367-326X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/138195
identifier_str_mv Mendieta, Julieta Renee; Figmonari, Carmela; Daleo, Gustavo Raul; Hrelia, Patrizia; Guevara, Maria Gabriela; Cytotoxic effect of potato aspartic proteases (StAPs) on Jurkat T cells; Elsevier Science; Fitoterapia; 81; 5; 13-10-2009; 329-335
0367-326X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fitote.2009.10.004
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0367326X09002494
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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