Interaction between facilitation and depression at a large CNS synapse reveals mechanisms of short-term plasticity
- Autores
- Müller, Martin; Goutman, Juan Diego; Kochubey, Olexiy; Schneggenburger, Ralf
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The two fundamental forms of short-term plasticity, short-term depression and facilitation, coexist at most synapses, but little is known about their interaction. Here, we studied the interplay between short-term depression and facilitation at calyx of Held synapses. Stimulation at a "low" frequency of 10 or 20 Hz, which is in the range of the spontaneous activity of these auditory neurons in vivo, induced synaptic depression. Surprisingly, an instantaneous increase of the stimulation frequency to 100 or 200 Hz following the low-frequency train uncovered a robust facilitation of EPSCs relative to the predepressed amplitude level. This facilitation decayed rapidly (∼30 ms) and depended on presynaptic residual Ca2+, but it was not caused by Ca2+ current facilitation. To probe the release probability of the remaining readily releasable vesicles following the low-frequency train we made presynaptic Ca2+ uncaging experiments in the predepressed state of the synapse. We found that low-frequency stimulation depletes the fast-releasable vesicle pool (FRP) down to ∼40% of control and that the remaining FRP vesicles are released with ∼2-fold slower release kinetics, indicating a hitherto unknown intrinsic heterogeneity among FRP vesicles. Thus, vesicles with an intrinsically lower release probability predominate after low frequency stimulation and undergo facilitation during the onset of subsequent high-frequency trains. Facilitation in the predepressed state of the synapse might help to stabilize the amount of transmitter release at the onset of high-frequency firing at these auditory synapses.
Fil: Müller, Martin. Ecole Polytechnique Federale de Lausanne; Suiza
Fil: Goutman, Juan Diego. Ecole Polytechnique Federale de Lausanne; Suiza. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kochubey, Olexiy. Ecole Polytechnique Federale de Lausanne; Suiza
Fil: Schneggenburger, Ralf. Ecole Polytechnique Federale de Lausanne; Suiza - Materia
-
synaptic transmission
facilitation
depression
exocytosis - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/53968
Ver los metadatos del registro completo
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Interaction between facilitation and depression at a large CNS synapse reveals mechanisms of short-term plasticityMüller, MartinGoutman, Juan DiegoKochubey, OlexiySchneggenburger, Ralfsynaptic transmissionfacilitationdepressionexocytosishttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The two fundamental forms of short-term plasticity, short-term depression and facilitation, coexist at most synapses, but little is known about their interaction. Here, we studied the interplay between short-term depression and facilitation at calyx of Held synapses. Stimulation at a "low" frequency of 10 or 20 Hz, which is in the range of the spontaneous activity of these auditory neurons in vivo, induced synaptic depression. Surprisingly, an instantaneous increase of the stimulation frequency to 100 or 200 Hz following the low-frequency train uncovered a robust facilitation of EPSCs relative to the predepressed amplitude level. This facilitation decayed rapidly (∼30 ms) and depended on presynaptic residual Ca2+, but it was not caused by Ca2+ current facilitation. To probe the release probability of the remaining readily releasable vesicles following the low-frequency train we made presynaptic Ca2+ uncaging experiments in the predepressed state of the synapse. We found that low-frequency stimulation depletes the fast-releasable vesicle pool (FRP) down to ∼40% of control and that the remaining FRP vesicles are released with ∼2-fold slower release kinetics, indicating a hitherto unknown intrinsic heterogeneity among FRP vesicles. Thus, vesicles with an intrinsically lower release probability predominate after low frequency stimulation and undergo facilitation during the onset of subsequent high-frequency trains. Facilitation in the predepressed state of the synapse might help to stabilize the amount of transmitter release at the onset of high-frequency firing at these auditory synapses.Fil: Müller, Martin. Ecole Polytechnique Federale de Lausanne; SuizaFil: Goutman, Juan Diego. Ecole Polytechnique Federale de Lausanne; Suiza. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Kochubey, Olexiy. Ecole Polytechnique Federale de Lausanne; SuizaFil: Schneggenburger, Ralf. Ecole Polytechnique Federale de Lausanne; SuizaSociety for Neuroscience2010-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53968Müller, Martin; Goutman, Juan Diego; Kochubey, Olexiy; Schneggenburger, Ralf; Interaction between facilitation and depression at a large CNS synapse reveals mechanisms of short-term plasticity; Society for Neuroscience; Journal of Neuroscience; 30; 6; 2-2010; 2007-20160270-6474CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1523/JNEUROSCI.4378-09.2010info:eu-repo/semantics/altIdentifier/url/http://www.jneurosci.org/content/30/6/2007info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:33:47Zoai:ri.conicet.gov.ar:11336/53968instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:33:47.3CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Interaction between facilitation and depression at a large CNS synapse reveals mechanisms of short-term plasticity |
title |
Interaction between facilitation and depression at a large CNS synapse reveals mechanisms of short-term plasticity |
spellingShingle |
Interaction between facilitation and depression at a large CNS synapse reveals mechanisms of short-term plasticity Müller, Martin synaptic transmission facilitation depression exocytosis |
title_short |
Interaction between facilitation and depression at a large CNS synapse reveals mechanisms of short-term plasticity |
title_full |
Interaction between facilitation and depression at a large CNS synapse reveals mechanisms of short-term plasticity |
title_fullStr |
Interaction between facilitation and depression at a large CNS synapse reveals mechanisms of short-term plasticity |
title_full_unstemmed |
Interaction between facilitation and depression at a large CNS synapse reveals mechanisms of short-term plasticity |
title_sort |
Interaction between facilitation and depression at a large CNS synapse reveals mechanisms of short-term plasticity |
dc.creator.none.fl_str_mv |
Müller, Martin Goutman, Juan Diego Kochubey, Olexiy Schneggenburger, Ralf |
author |
Müller, Martin |
author_facet |
Müller, Martin Goutman, Juan Diego Kochubey, Olexiy Schneggenburger, Ralf |
author_role |
author |
author2 |
Goutman, Juan Diego Kochubey, Olexiy Schneggenburger, Ralf |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
synaptic transmission facilitation depression exocytosis |
topic |
synaptic transmission facilitation depression exocytosis |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
The two fundamental forms of short-term plasticity, short-term depression and facilitation, coexist at most synapses, but little is known about their interaction. Here, we studied the interplay between short-term depression and facilitation at calyx of Held synapses. Stimulation at a "low" frequency of 10 or 20 Hz, which is in the range of the spontaneous activity of these auditory neurons in vivo, induced synaptic depression. Surprisingly, an instantaneous increase of the stimulation frequency to 100 or 200 Hz following the low-frequency train uncovered a robust facilitation of EPSCs relative to the predepressed amplitude level. This facilitation decayed rapidly (∼30 ms) and depended on presynaptic residual Ca2+, but it was not caused by Ca2+ current facilitation. To probe the release probability of the remaining readily releasable vesicles following the low-frequency train we made presynaptic Ca2+ uncaging experiments in the predepressed state of the synapse. We found that low-frequency stimulation depletes the fast-releasable vesicle pool (FRP) down to ∼40% of control and that the remaining FRP vesicles are released with ∼2-fold slower release kinetics, indicating a hitherto unknown intrinsic heterogeneity among FRP vesicles. Thus, vesicles with an intrinsically lower release probability predominate after low frequency stimulation and undergo facilitation during the onset of subsequent high-frequency trains. Facilitation in the predepressed state of the synapse might help to stabilize the amount of transmitter release at the onset of high-frequency firing at these auditory synapses. Fil: Müller, Martin. Ecole Polytechnique Federale de Lausanne; Suiza Fil: Goutman, Juan Diego. Ecole Polytechnique Federale de Lausanne; Suiza. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Kochubey, Olexiy. Ecole Polytechnique Federale de Lausanne; Suiza Fil: Schneggenburger, Ralf. Ecole Polytechnique Federale de Lausanne; Suiza |
description |
The two fundamental forms of short-term plasticity, short-term depression and facilitation, coexist at most synapses, but little is known about their interaction. Here, we studied the interplay between short-term depression and facilitation at calyx of Held synapses. Stimulation at a "low" frequency of 10 or 20 Hz, which is in the range of the spontaneous activity of these auditory neurons in vivo, induced synaptic depression. Surprisingly, an instantaneous increase of the stimulation frequency to 100 or 200 Hz following the low-frequency train uncovered a robust facilitation of EPSCs relative to the predepressed amplitude level. This facilitation decayed rapidly (∼30 ms) and depended on presynaptic residual Ca2+, but it was not caused by Ca2+ current facilitation. To probe the release probability of the remaining readily releasable vesicles following the low-frequency train we made presynaptic Ca2+ uncaging experiments in the predepressed state of the synapse. We found that low-frequency stimulation depletes the fast-releasable vesicle pool (FRP) down to ∼40% of control and that the remaining FRP vesicles are released with ∼2-fold slower release kinetics, indicating a hitherto unknown intrinsic heterogeneity among FRP vesicles. Thus, vesicles with an intrinsically lower release probability predominate after low frequency stimulation and undergo facilitation during the onset of subsequent high-frequency trains. Facilitation in the predepressed state of the synapse might help to stabilize the amount of transmitter release at the onset of high-frequency firing at these auditory synapses. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/53968 Müller, Martin; Goutman, Juan Diego; Kochubey, Olexiy; Schneggenburger, Ralf; Interaction between facilitation and depression at a large CNS synapse reveals mechanisms of short-term plasticity; Society for Neuroscience; Journal of Neuroscience; 30; 6; 2-2010; 2007-2016 0270-6474 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/53968 |
identifier_str_mv |
Müller, Martin; Goutman, Juan Diego; Kochubey, Olexiy; Schneggenburger, Ralf; Interaction between facilitation and depression at a large CNS synapse reveals mechanisms of short-term plasticity; Society for Neuroscience; Journal of Neuroscience; 30; 6; 2-2010; 2007-2016 0270-6474 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1523/JNEUROSCI.4378-09.2010 info:eu-repo/semantics/altIdentifier/url/http://www.jneurosci.org/content/30/6/2007 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Society for Neuroscience |
publisher.none.fl_str_mv |
Society for Neuroscience |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614353319362560 |
score |
13.070432 |