GnRH neurones population in the diencephalon of the coypu (Myocastor coypus)

Autores
Silva, L.; Sánchez, H.; Acosta, W.; Portiansky, Enrique Leo; Zuccolilli, Gustavo Oscar
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The goal of this study was to investigate the distribution and morphology of neurones that synthesise and store gonadotrophin releasing factor (GnRH) in the diencephalon of the coypu (Myocastor coypus), a South American rodent of the hystricomorpha suborder. For this purpose the encephalon of three mature male coypus were fixed by intra-arterial perfusion, using a paraformaldehyde and picric acid solution. The hypothalamic blocks were cut with freezing microtome to obtain 40µm thick coronal sections. The slides were exposed to an ultrasound antigen retrieval process in order to unmask hidden antigens. These sections were then processed using an immunohistochemical technique to show the GnRH neurones, using a monoclonal antibody (LRH 13). One group of serial sections was stained with cresyl violet (Nissl techniques) while the other sections were stained using Küver Barrera´s method (luxol fast blue and cresyl violet), to show the nuclei and nervous tracts of the hypothalamus under microscopic observation. Morphometric and quantitative analysis of neuronal bodies were performed using an image analyser. GnRH immunoreactive neurones were bipolar and long. The total number of neuronal bodies for this species was estimated at 1072 ± 27. All the cells were located in the rostral hypothalamus, mainly in the preoptic area. A few neurones were also observed in the bed nucleus of the stria terminalis and hypothalamic preoptic medial nucleus. The immunoreactive fibres were observed in the external layer of the median eminence. According to the data obtained we inferred that the distribution of the GnRH neurones of the coypu mainly coincides with that of other rodents such as the rat.
Fil: Silva, L.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Sánchez, H.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Acosta, W.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Portiansky, Enrique Leo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Zuccolilli, Gustavo Oscar. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Materia
Coypu
Gnrh
Hypothalamus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53578
Acceder
id CONICETDig_77e0905212d4cd06ded8c66683758dd5
oai_identifier_str oai:ri.conicet.gov.ar:11336/53578
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling GnRH neurones population in the diencephalon of the coypu (Myocastor coypus)Estudio inmunohistoquímico de la población de neuronas gnrh en el diencéfalo del coipo (Myocastor coypus)Silva, L.Sánchez, H.Acosta, W.Portiansky, Enrique LeoZuccolilli, Gustavo OscarCoypuGnrhHypothalamushttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The goal of this study was to investigate the distribution and morphology of neurones that synthesise and store gonadotrophin releasing factor (GnRH) in the diencephalon of the coypu (Myocastor coypus), a South American rodent of the hystricomorpha suborder. For this purpose the encephalon of three mature male coypus were fixed by intra-arterial perfusion, using a paraformaldehyde and picric acid solution. The hypothalamic blocks were cut with freezing microtome to obtain 40µm thick coronal sections. The slides were exposed to an ultrasound antigen retrieval process in order to unmask hidden antigens. These sections were then processed using an immunohistochemical technique to show the GnRH neurones, using a monoclonal antibody (LRH 13). One group of serial sections was stained with cresyl violet (Nissl techniques) while the other sections were stained using Küver Barrera´s method (luxol fast blue and cresyl violet), to show the nuclei and nervous tracts of the hypothalamus under microscopic observation. Morphometric and quantitative analysis of neuronal bodies were performed using an image analyser. GnRH immunoreactive neurones were bipolar and long. The total number of neuronal bodies for this species was estimated at 1072 ± 27. All the cells were located in the rostral hypothalamus, mainly in the preoptic area. A few neurones were also observed in the bed nucleus of the stria terminalis and hypothalamic preoptic medial nucleus. The immunoreactive fibres were observed in the external layer of the median eminence. According to the data obtained we inferred that the distribution of the GnRH neurones of the coypu mainly coincides with that of other rodents such as the rat.Fil: Silva, L.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Sánchez, H.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Acosta, W.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Portiansky, Enrique Leo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Zuccolilli, Gustavo Oscar. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaSociedad Chilena de Anatomía2000-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53578Silva, L.; Sánchez, H.; Acosta, W.; Portiansky, Enrique Leo; Zuccolilli, Gustavo Oscar; GnRH neurones population in the diencephalon of the coypu (Myocastor coypus); Sociedad Chilena de Anatomía; REVISTA CHILENA DE ANATOMíA; 18; 1; 1-2000; 5-110717-6244CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4067/S0716-98682000000100001info:eu-repo/semantics/altIdentifier/url/https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-98682000000100001&lng=en&nrm=iso&tlng=eninfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:42:54Zoai:ri.conicet.gov.ar:11336/53578instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:42:54.414CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv GnRH neurones population in the diencephalon of the coypu (Myocastor coypus)
Estudio inmunohistoquímico de la población de neuronas gnrh en el diencéfalo del coipo (Myocastor coypus)
title GnRH neurones population in the diencephalon of the coypu (Myocastor coypus)
spellingShingle GnRH neurones population in the diencephalon of the coypu (Myocastor coypus)
Silva, L.
Coypu
Gnrh
Hypothalamus
title_short GnRH neurones population in the diencephalon of the coypu (Myocastor coypus)
title_full GnRH neurones population in the diencephalon of the coypu (Myocastor coypus)
title_fullStr GnRH neurones population in the diencephalon of the coypu (Myocastor coypus)
title_full_unstemmed GnRH neurones population in the diencephalon of the coypu (Myocastor coypus)
title_sort GnRH neurones population in the diencephalon of the coypu (Myocastor coypus)
dc.creator.none.fl_str_mv Silva, L.
Sánchez, H.
Acosta, W.
Portiansky, Enrique Leo
Zuccolilli, Gustavo Oscar
author Silva, L.
author_facet Silva, L.
Sánchez, H.
Acosta, W.
Portiansky, Enrique Leo
Zuccolilli, Gustavo Oscar
author_role author
author2 Sánchez, H.
Acosta, W.
Portiansky, Enrique Leo
Zuccolilli, Gustavo Oscar
author2_role author
author
author
author
dc.subject.none.fl_str_mv Coypu
Gnrh
Hypothalamus
topic Coypu
Gnrh
Hypothalamus
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The goal of this study was to investigate the distribution and morphology of neurones that synthesise and store gonadotrophin releasing factor (GnRH) in the diencephalon of the coypu (Myocastor coypus), a South American rodent of the hystricomorpha suborder. For this purpose the encephalon of three mature male coypus were fixed by intra-arterial perfusion, using a paraformaldehyde and picric acid solution. The hypothalamic blocks were cut with freezing microtome to obtain 40µm thick coronal sections. The slides were exposed to an ultrasound antigen retrieval process in order to unmask hidden antigens. These sections were then processed using an immunohistochemical technique to show the GnRH neurones, using a monoclonal antibody (LRH 13). One group of serial sections was stained with cresyl violet (Nissl techniques) while the other sections were stained using Küver Barrera´s method (luxol fast blue and cresyl violet), to show the nuclei and nervous tracts of the hypothalamus under microscopic observation. Morphometric and quantitative analysis of neuronal bodies were performed using an image analyser. GnRH immunoreactive neurones were bipolar and long. The total number of neuronal bodies for this species was estimated at 1072 ± 27. All the cells were located in the rostral hypothalamus, mainly in the preoptic area. A few neurones were also observed in the bed nucleus of the stria terminalis and hypothalamic preoptic medial nucleus. The immunoreactive fibres were observed in the external layer of the median eminence. According to the data obtained we inferred that the distribution of the GnRH neurones of the coypu mainly coincides with that of other rodents such as the rat.
Fil: Silva, L.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Sánchez, H.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Acosta, W.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Portiansky, Enrique Leo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Zuccolilli, Gustavo Oscar. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
description The goal of this study was to investigate the distribution and morphology of neurones that synthesise and store gonadotrophin releasing factor (GnRH) in the diencephalon of the coypu (Myocastor coypus), a South American rodent of the hystricomorpha suborder. For this purpose the encephalon of three mature male coypus were fixed by intra-arterial perfusion, using a paraformaldehyde and picric acid solution. The hypothalamic blocks were cut with freezing microtome to obtain 40µm thick coronal sections. The slides were exposed to an ultrasound antigen retrieval process in order to unmask hidden antigens. These sections were then processed using an immunohistochemical technique to show the GnRH neurones, using a monoclonal antibody (LRH 13). One group of serial sections was stained with cresyl violet (Nissl techniques) while the other sections were stained using Küver Barrera´s method (luxol fast blue and cresyl violet), to show the nuclei and nervous tracts of the hypothalamus under microscopic observation. Morphometric and quantitative analysis of neuronal bodies were performed using an image analyser. GnRH immunoreactive neurones were bipolar and long. The total number of neuronal bodies for this species was estimated at 1072 ± 27. All the cells were located in the rostral hypothalamus, mainly in the preoptic area. A few neurones were also observed in the bed nucleus of the stria terminalis and hypothalamic preoptic medial nucleus. The immunoreactive fibres were observed in the external layer of the median eminence. According to the data obtained we inferred that the distribution of the GnRH neurones of the coypu mainly coincides with that of other rodents such as the rat.
publishDate 2000
dc.date.none.fl_str_mv 2000-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53578
Silva, L.; Sánchez, H.; Acosta, W.; Portiansky, Enrique Leo; Zuccolilli, Gustavo Oscar; GnRH neurones population in the diencephalon of the coypu (Myocastor coypus); Sociedad Chilena de Anatomía; REVISTA CHILENA DE ANATOMíA; 18; 1; 1-2000; 5-11
0717-6244
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53578
identifier_str_mv Silva, L.; Sánchez, H.; Acosta, W.; Portiansky, Enrique Leo; Zuccolilli, Gustavo Oscar; GnRH neurones population in the diencephalon of the coypu (Myocastor coypus); Sociedad Chilena de Anatomía; REVISTA CHILENA DE ANATOMíA; 18; 1; 1-2000; 5-11
0717-6244
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4067/S0716-98682000000100001
info:eu-repo/semantics/altIdentifier/url/https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-98682000000100001&lng=en&nrm=iso&tlng=en
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedad Chilena de Anatomía
publisher.none.fl_str_mv Sociedad Chilena de Anatomía
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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