Distribution of tyrosine Hydroxylase-Immunoreactive neurones in the diencephalon and mesencephalon of the coypu (Myocastor coypus)

Autores
Sánchez, H. L.; Silva, Liliana Beatriz; Acosta, Walter Gustavo; Portiansky, Enrique Leo; Zuccolilli, Gustavo Oscar
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the present study was to examine the catecholaminergic neurones located within the midbrain of the coypu, a South American hystricomorph rodent. The neuronal distribution of the catecholaminergic systems and morphological parameters of the immunostained cell bodies and fibres were investigated, using an immunohistochemical method. The brains of five coypu were fixed, immersed in gelatine-glycerol and cut in 40-μm slices using a freezing microtome. Samples were processed with ultrasound-based antigen retrieval and stained with labelled antityrosine hydroxylase monoclonal antibody. An image analyser was used to measure the neuronal bodies. The catecholaminergic neurones of the tuberoinfundibular system were mainly observed in the arcuate and periventricular nuclei with their axons projecting towards to the median eminence; they represented 28% of the global population of tyrosine hydroxylase-immunoreactive cells observed. Significant morphological differences were observed in comparison with the other two studied systems. Fifty per cent of total catecholaminergic neurones were detected in the nigrostriatal system distributed in the reticular and compact substance nigra. Most neuronal bodies had a fusiform aspect. The immunoreactive neurones of the mesolimbic system represented 22% of the total population. They were distributed around the interpeduncular nucleus. Two types of morphologically different catecholaminergic systems of the brain were established: hypothalamic neurones located in the periventricular and arcuate nuclei and mesencephalic neurones located in the substance nigra and interpeduncular nuclei. These systems showed morphological and probably physiological-pharmacological differences.
Fil: Sánchez, H. L.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Silva, Liliana Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Acosta, Walter Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Portiansky, Enrique Leo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zuccolilli, Gustavo Oscar. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Materia
Coypu
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53437
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/53437
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Distribution of tyrosine Hydroxylase-Immunoreactive neurones in the diencephalon and mesencephalon of the coypu (Myocastor coypus)Sánchez, H. L.Silva, Liliana BeatrizAcosta, Walter GustavoPortiansky, Enrique LeoZuccolilli, Gustavo OscarCoypuhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aim of the present study was to examine the catecholaminergic neurones located within the midbrain of the coypu, a South American hystricomorph rodent. The neuronal distribution of the catecholaminergic systems and morphological parameters of the immunostained cell bodies and fibres were investigated, using an immunohistochemical method. The brains of five coypu were fixed, immersed in gelatine-glycerol and cut in 40-μm slices using a freezing microtome. Samples were processed with ultrasound-based antigen retrieval and stained with labelled antityrosine hydroxylase monoclonal antibody. An image analyser was used to measure the neuronal bodies. The catecholaminergic neurones of the tuberoinfundibular system were mainly observed in the arcuate and periventricular nuclei with their axons projecting towards to the median eminence; they represented 28% of the global population of tyrosine hydroxylase-immunoreactive cells observed. Significant morphological differences were observed in comparison with the other two studied systems. Fifty per cent of total catecholaminergic neurones were detected in the nigrostriatal system distributed in the reticular and compact substance nigra. Most neuronal bodies had a fusiform aspect. The immunoreactive neurones of the mesolimbic system represented 22% of the total population. They were distributed around the interpeduncular nucleus. Two types of morphologically different catecholaminergic systems of the brain were established: hypothalamic neurones located in the periventricular and arcuate nuclei and mesencephalic neurones located in the substance nigra and interpeduncular nuclei. These systems showed morphological and probably physiological-pharmacological differences.Fil: Sánchez, H. L.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Silva, Liliana Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Acosta, Walter Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Portiansky, Enrique Leo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zuccolilli, Gustavo Oscar. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaWiley VCH Verlag2000-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53437Sánchez, H. L.; Silva, Liliana Beatriz; Acosta, Walter Gustavo; Portiansky, Enrique Leo; Zuccolilli, Gustavo Oscar; Distribution of tyrosine Hydroxylase-Immunoreactive neurones in the diencephalon and mesencephalon of the coypu (Myocastor coypus); Wiley VCH Verlag; Anatomia Histologia Embryologia-journal Of Veterinary Medicine Series C; 29; 6; 12-2000; 375-3800340-2096CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1046/j.1439-0264.2000.00285.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1046/j.1439-0264.2000.00285.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:28:44Zoai:ri.conicet.gov.ar:11336/53437instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:28:44.657CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Distribution of tyrosine Hydroxylase-Immunoreactive neurones in the diencephalon and mesencephalon of the coypu (Myocastor coypus)
title Distribution of tyrosine Hydroxylase-Immunoreactive neurones in the diencephalon and mesencephalon of the coypu (Myocastor coypus)
spellingShingle Distribution of tyrosine Hydroxylase-Immunoreactive neurones in the diencephalon and mesencephalon of the coypu (Myocastor coypus)
Sánchez, H. L.
Coypu
title_short Distribution of tyrosine Hydroxylase-Immunoreactive neurones in the diencephalon and mesencephalon of the coypu (Myocastor coypus)
title_full Distribution of tyrosine Hydroxylase-Immunoreactive neurones in the diencephalon and mesencephalon of the coypu (Myocastor coypus)
title_fullStr Distribution of tyrosine Hydroxylase-Immunoreactive neurones in the diencephalon and mesencephalon of the coypu (Myocastor coypus)
title_full_unstemmed Distribution of tyrosine Hydroxylase-Immunoreactive neurones in the diencephalon and mesencephalon of the coypu (Myocastor coypus)
title_sort Distribution of tyrosine Hydroxylase-Immunoreactive neurones in the diencephalon and mesencephalon of the coypu (Myocastor coypus)
dc.creator.none.fl_str_mv Sánchez, H. L.
Silva, Liliana Beatriz
Acosta, Walter Gustavo
Portiansky, Enrique Leo
Zuccolilli, Gustavo Oscar
author Sánchez, H. L.
author_facet Sánchez, H. L.
Silva, Liliana Beatriz
Acosta, Walter Gustavo
Portiansky, Enrique Leo
Zuccolilli, Gustavo Oscar
author_role author
author2 Silva, Liliana Beatriz
Acosta, Walter Gustavo
Portiansky, Enrique Leo
Zuccolilli, Gustavo Oscar
author2_role author
author
author
author
dc.subject.none.fl_str_mv Coypu
topic Coypu
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aim of the present study was to examine the catecholaminergic neurones located within the midbrain of the coypu, a South American hystricomorph rodent. The neuronal distribution of the catecholaminergic systems and morphological parameters of the immunostained cell bodies and fibres were investigated, using an immunohistochemical method. The brains of five coypu were fixed, immersed in gelatine-glycerol and cut in 40-μm slices using a freezing microtome. Samples were processed with ultrasound-based antigen retrieval and stained with labelled antityrosine hydroxylase monoclonal antibody. An image analyser was used to measure the neuronal bodies. The catecholaminergic neurones of the tuberoinfundibular system were mainly observed in the arcuate and periventricular nuclei with their axons projecting towards to the median eminence; they represented 28% of the global population of tyrosine hydroxylase-immunoreactive cells observed. Significant morphological differences were observed in comparison with the other two studied systems. Fifty per cent of total catecholaminergic neurones were detected in the nigrostriatal system distributed in the reticular and compact substance nigra. Most neuronal bodies had a fusiform aspect. The immunoreactive neurones of the mesolimbic system represented 22% of the total population. They were distributed around the interpeduncular nucleus. Two types of morphologically different catecholaminergic systems of the brain were established: hypothalamic neurones located in the periventricular and arcuate nuclei and mesencephalic neurones located in the substance nigra and interpeduncular nuclei. These systems showed morphological and probably physiological-pharmacological differences.
Fil: Sánchez, H. L.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Silva, Liliana Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Acosta, Walter Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Portiansky, Enrique Leo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zuccolilli, Gustavo Oscar. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
description The aim of the present study was to examine the catecholaminergic neurones located within the midbrain of the coypu, a South American hystricomorph rodent. The neuronal distribution of the catecholaminergic systems and morphological parameters of the immunostained cell bodies and fibres were investigated, using an immunohistochemical method. The brains of five coypu were fixed, immersed in gelatine-glycerol and cut in 40-μm slices using a freezing microtome. Samples were processed with ultrasound-based antigen retrieval and stained with labelled antityrosine hydroxylase monoclonal antibody. An image analyser was used to measure the neuronal bodies. The catecholaminergic neurones of the tuberoinfundibular system were mainly observed in the arcuate and periventricular nuclei with their axons projecting towards to the median eminence; they represented 28% of the global population of tyrosine hydroxylase-immunoreactive cells observed. Significant morphological differences were observed in comparison with the other two studied systems. Fifty per cent of total catecholaminergic neurones were detected in the nigrostriatal system distributed in the reticular and compact substance nigra. Most neuronal bodies had a fusiform aspect. The immunoreactive neurones of the mesolimbic system represented 22% of the total population. They were distributed around the interpeduncular nucleus. Two types of morphologically different catecholaminergic systems of the brain were established: hypothalamic neurones located in the periventricular and arcuate nuclei and mesencephalic neurones located in the substance nigra and interpeduncular nuclei. These systems showed morphological and probably physiological-pharmacological differences.
publishDate 2000
dc.date.none.fl_str_mv 2000-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53437
Sánchez, H. L.; Silva, Liliana Beatriz; Acosta, Walter Gustavo; Portiansky, Enrique Leo; Zuccolilli, Gustavo Oscar; Distribution of tyrosine Hydroxylase-Immunoreactive neurones in the diencephalon and mesencephalon of the coypu (Myocastor coypus); Wiley VCH Verlag; Anatomia Histologia Embryologia-journal Of Veterinary Medicine Series C; 29; 6; 12-2000; 375-380
0340-2096
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53437
identifier_str_mv Sánchez, H. L.; Silva, Liliana Beatriz; Acosta, Walter Gustavo; Portiansky, Enrique Leo; Zuccolilli, Gustavo Oscar; Distribution of tyrosine Hydroxylase-Immunoreactive neurones in the diencephalon and mesencephalon of the coypu (Myocastor coypus); Wiley VCH Verlag; Anatomia Histologia Embryologia-journal Of Veterinary Medicine Series C; 29; 6; 12-2000; 375-380
0340-2096
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1046/j.1439-0264.2000.00285.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1046/j.1439-0264.2000.00285.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083427537780736
score 13.22299

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