Specific enumeration and analysis of the community structure of culturable pseudomonads in agricultural soils under no-till management in Argentina

Autores
Agaras, Betina Cecilia; Wall, Luis Gabriel; Valverde, Claudio Fabián
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Members of the Pseudomonas genus have been isolated and identified worldwide as plant probiotic microorganisms. Little is known about the dynamics of pseudomonads population in extensive agricultural systems under the influence of biotic and abiotic factors. As part of the national consortium BIOSPAS devoted to study soil biology and its relationship with productivity, our lab began to characterize pseudomonad populations in agricultural soils under no-till management. We here report the set up and application of a combination of selective plating and PCR-RFLP analysis of the genus specific gacA and oprF genes, to quantify and study the community structure of culturable pseudomonads in bulk soil and rhizosphere samples. The specificity of both methods has been verified by 16S rDNA and oprF sequencing. The application of selective plating and gacA/oprF PCR-RFLP analyses revealed differences in the amount and predominant type of culturable pseudomonads among geographical locations, among treatments, and between rhizospheric and bulk soil. The results indicate that these simple methods prove useful to systematically monitor pseudomonads structure in bulk and rhizospheric soils along sampling periods covering crop rotations and agricultural practices at different locations.
Fil: Agaras, Betina Cecilia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Interacciones Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wall, Luis Gabriel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Interacciones Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valverde, Claudio Fabián. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Interacciones Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
COMMUNITY STRUCTURE
GACA
NO-TILLAGE
OPRF
PSEUDOMONAS
RHIZOSPHERE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/197934
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/197934
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Specific enumeration and analysis of the community structure of culturable pseudomonads in agricultural soils under no-till management in ArgentinaAgaras, Betina CeciliaWall, Luis GabrielValverde, Claudio FabiánCOMMUNITY STRUCTUREGACANO-TILLAGEOPRFPSEUDOMONASRHIZOSPHEREhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Members of the Pseudomonas genus have been isolated and identified worldwide as plant probiotic microorganisms. Little is known about the dynamics of pseudomonads population in extensive agricultural systems under the influence of biotic and abiotic factors. As part of the national consortium BIOSPAS devoted to study soil biology and its relationship with productivity, our lab began to characterize pseudomonad populations in agricultural soils under no-till management. We here report the set up and application of a combination of selective plating and PCR-RFLP analysis of the genus specific gacA and oprF genes, to quantify and study the community structure of culturable pseudomonads in bulk soil and rhizosphere samples. The specificity of both methods has been verified by 16S rDNA and oprF sequencing. The application of selective plating and gacA/oprF PCR-RFLP analyses revealed differences in the amount and predominant type of culturable pseudomonads among geographical locations, among treatments, and between rhizospheric and bulk soil. The results indicate that these simple methods prove useful to systematically monitor pseudomonads structure in bulk and rhizospheric soils along sampling periods covering crop rotations and agricultural practices at different locations.Fil: Agaras, Betina Cecilia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Interacciones Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Wall, Luis Gabriel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Interacciones Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Valverde, Claudio Fabián. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Interacciones Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2012-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/197934Agaras, Betina Cecilia; Wall, Luis Gabriel; Valverde, Claudio Fabián; Specific enumeration and analysis of the community structure of culturable pseudomonads in agricultural soils under no-till management in Argentina; Elsevier Science; Applied Soil Ecology; 61; 10-2012; 305-3190929-1393CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0929139311003210info:eu-repo/semantics/altIdentifier/doi/10.1016/j.apsoil.2011.11.016info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:01:33Zoai:ri.conicet.gov.ar:11336/197934instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:01:33.885CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Specific enumeration and analysis of the community structure of culturable pseudomonads in agricultural soils under no-till management in Argentina
title Specific enumeration and analysis of the community structure of culturable pseudomonads in agricultural soils under no-till management in Argentina
spellingShingle Specific enumeration and analysis of the community structure of culturable pseudomonads in agricultural soils under no-till management in Argentina
Agaras, Betina Cecilia
COMMUNITY STRUCTURE
GACA
NO-TILLAGE
OPRF
PSEUDOMONAS
RHIZOSPHERE
title_short Specific enumeration and analysis of the community structure of culturable pseudomonads in agricultural soils under no-till management in Argentina
title_full Specific enumeration and analysis of the community structure of culturable pseudomonads in agricultural soils under no-till management in Argentina
title_fullStr Specific enumeration and analysis of the community structure of culturable pseudomonads in agricultural soils under no-till management in Argentina
title_full_unstemmed Specific enumeration and analysis of the community structure of culturable pseudomonads in agricultural soils under no-till management in Argentina
title_sort Specific enumeration and analysis of the community structure of culturable pseudomonads in agricultural soils under no-till management in Argentina
dc.creator.none.fl_str_mv Agaras, Betina Cecilia
Wall, Luis Gabriel
Valverde, Claudio Fabián
author Agaras, Betina Cecilia
author_facet Agaras, Betina Cecilia
Wall, Luis Gabriel
Valverde, Claudio Fabián
author_role author
author2 Wall, Luis Gabriel
Valverde, Claudio Fabián
author2_role author
author
dc.subject.none.fl_str_mv COMMUNITY STRUCTURE
GACA
NO-TILLAGE
OPRF
PSEUDOMONAS
RHIZOSPHERE
topic COMMUNITY STRUCTURE
GACA
NO-TILLAGE
OPRF
PSEUDOMONAS
RHIZOSPHERE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Members of the Pseudomonas genus have been isolated and identified worldwide as plant probiotic microorganisms. Little is known about the dynamics of pseudomonads population in extensive agricultural systems under the influence of biotic and abiotic factors. As part of the national consortium BIOSPAS devoted to study soil biology and its relationship with productivity, our lab began to characterize pseudomonad populations in agricultural soils under no-till management. We here report the set up and application of a combination of selective plating and PCR-RFLP analysis of the genus specific gacA and oprF genes, to quantify and study the community structure of culturable pseudomonads in bulk soil and rhizosphere samples. The specificity of both methods has been verified by 16S rDNA and oprF sequencing. The application of selective plating and gacA/oprF PCR-RFLP analyses revealed differences in the amount and predominant type of culturable pseudomonads among geographical locations, among treatments, and between rhizospheric and bulk soil. The results indicate that these simple methods prove useful to systematically monitor pseudomonads structure in bulk and rhizospheric soils along sampling periods covering crop rotations and agricultural practices at different locations.
Fil: Agaras, Betina Cecilia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Interacciones Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wall, Luis Gabriel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Interacciones Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valverde, Claudio Fabián. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Interacciones Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Members of the Pseudomonas genus have been isolated and identified worldwide as plant probiotic microorganisms. Little is known about the dynamics of pseudomonads population in extensive agricultural systems under the influence of biotic and abiotic factors. As part of the national consortium BIOSPAS devoted to study soil biology and its relationship with productivity, our lab began to characterize pseudomonad populations in agricultural soils under no-till management. We here report the set up and application of a combination of selective plating and PCR-RFLP analysis of the genus specific gacA and oprF genes, to quantify and study the community structure of culturable pseudomonads in bulk soil and rhizosphere samples. The specificity of both methods has been verified by 16S rDNA and oprF sequencing. The application of selective plating and gacA/oprF PCR-RFLP analyses revealed differences in the amount and predominant type of culturable pseudomonads among geographical locations, among treatments, and between rhizospheric and bulk soil. The results indicate that these simple methods prove useful to systematically monitor pseudomonads structure in bulk and rhizospheric soils along sampling periods covering crop rotations and agricultural practices at different locations.
publishDate 2012
dc.date.none.fl_str_mv 2012-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/197934
Agaras, Betina Cecilia; Wall, Luis Gabriel; Valverde, Claudio Fabián; Specific enumeration and analysis of the community structure of culturable pseudomonads in agricultural soils under no-till management in Argentina; Elsevier Science; Applied Soil Ecology; 61; 10-2012; 305-319
0929-1393
CONICET Digital
CONICET
url http://hdl.handle.net/11336/197934
identifier_str_mv Agaras, Betina Cecilia; Wall, Luis Gabriel; Valverde, Claudio Fabián; Specific enumeration and analysis of the community structure of culturable pseudomonads in agricultural soils under no-till management in Argentina; Elsevier Science; Applied Soil Ecology; 61; 10-2012; 305-319
0929-1393
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0929139311003210
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.apsoil.2011.11.016
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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