Arctic char ( Salvelinus alpinus ) metallothionein: cDNA sequence, expression, and tissue-specific inhibition of cadmium-mediated metallothionein induction by 17β-estradiol, 4-OH-P...

Autores
Gerpe, Marcela Silvia; Kling, Peter; Berg, Axel Håkan; Olsson, Per Erik
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study, we haveexamined the basallevel expression and tissue‐specificexpressionpatternsof metallothionein (MT) in Arctic char following metal and E2 (17β‐estradiol) treatment. To study the gene regulation in Arctic char, the two MT isoforms were isolated from a lambda‐ZAP hepatic cDNA library and characterized. Determination of basal MT mRNA and MT expression for 10 different tissues revealed a lack of correlation between MT mRNA and MT levels. The inducibility of MT mRNA and the correlation to resulting MT levels were then determined for liver and kidney. We found a more rapid and stronger induction of MT mRNA in liver than in kidney at day 1 and 3 postinjection, whereas the MT protein quantification showed higher MT levels in kidney than in liver at days 3 and 7 postinjection. These discrepancies indicate that differences in metal handling or posttran‐scriptional regulation of MT exists between tissues. Whereas metals induce MT synthesis, E2 inhibit the hepatic MT expression. To examine the tissue specificity of this inhibition, we determined the effect of 17pj‐estradiol (E2) and two estrogenic PCBs (4′‐OH‐PCB 30 and PCB 104) on Cd‐mediated MT induction in liver and kidney. Although E2 and the estrogenic PCBs inhibited cadmium‐mediated hepatic MT induction, these compounds did not interfere with renal MT induction.
Fil: Gerpe, Marcela Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Kling, Peter. Umeå University; Suecia
Fil: Berg, Axel Håkan. Umeå University; Suecia
Fil: Olsson, Per Erik. Umeå University; Suecia
Materia
Metallothionein
Arcticchar
Estrogen
Polychlorinated Biphenyls
Toxicology
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/69163
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/69163
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Arctic char ( Salvelinus alpinus ) metallothionein: cDNA sequence, expression, and tissue-specific inhibition of cadmium-mediated metallothionein induction by 17β-estradiol, 4-OH-PCB 30, and PCB 104Gerpe, Marcela SilviaKling, PeterBerg, Axel HåkanOlsson, Per ErikMetallothioneinArcticcharEstrogenPolychlorinated BiphenylsToxicologyhttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1In this study, we haveexamined the basallevel expression and tissue‐specificexpressionpatternsof metallothionein (MT) in Arctic char following metal and E2 (17β‐estradiol) treatment. To study the gene regulation in Arctic char, the two MT isoforms were isolated from a lambda‐ZAP hepatic cDNA library and characterized. Determination of basal MT mRNA and MT expression for 10 different tissues revealed a lack of correlation between MT mRNA and MT levels. The inducibility of MT mRNA and the correlation to resulting MT levels were then determined for liver and kidney. We found a more rapid and stronger induction of MT mRNA in liver than in kidney at day 1 and 3 postinjection, whereas the MT protein quantification showed higher MT levels in kidney than in liver at days 3 and 7 postinjection. These discrepancies indicate that differences in metal handling or posttran‐scriptional regulation of MT exists between tissues. Whereas metals induce MT synthesis, E2 inhibit the hepatic MT expression. To examine the tissue specificity of this inhibition, we determined the effect of 17pj‐estradiol (E2) and two estrogenic PCBs (4′‐OH‐PCB 30 and PCB 104) on Cd‐mediated MT induction in liver and kidney. Although E2 and the estrogenic PCBs inhibited cadmium‐mediated hepatic MT induction, these compounds did not interfere with renal MT induction.Fil: Gerpe, Marcela Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Kling, Peter. Umeå University; SueciaFil: Berg, Axel Håkan. Umeå University; SueciaFil: Olsson, Per Erik. Umeå University; SueciaSociety of Environmental Toxicology and Chemistry2000-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/69163Gerpe, Marcela Silvia; Kling, Peter; Berg, Axel Håkan; Olsson, Per Erik; Arctic char ( Salvelinus alpinus ) metallothionein: cDNA sequence, expression, and tissue-specific inhibition of cadmium-mediated metallothionein induction by 17β-estradiol, 4-OH-PCB 30, and PCB 104; Society of Environmental Toxicology and Chemistry; Environmental Toxicology and Chemistry; 19; 3; 3-2000; 638-6450730-7268CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/etc.5620190316info:eu-repo/semantics/altIdentifier/url/https://setac.onlinelibrary.wiley.com/doi/abs/10.1002/etc.5620190316info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:37:04Zoai:ri.conicet.gov.ar:11336/69163instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:37:04.876CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Arctic char ( Salvelinus alpinus ) metallothionein: cDNA sequence, expression, and tissue-specific inhibition of cadmium-mediated metallothionein induction by 17β-estradiol, 4-OH-PCB 30, and PCB 104
title Arctic char ( Salvelinus alpinus ) metallothionein: cDNA sequence, expression, and tissue-specific inhibition of cadmium-mediated metallothionein induction by 17β-estradiol, 4-OH-PCB 30, and PCB 104
spellingShingle Arctic char ( Salvelinus alpinus ) metallothionein: cDNA sequence, expression, and tissue-specific inhibition of cadmium-mediated metallothionein induction by 17β-estradiol, 4-OH-PCB 30, and PCB 104
Gerpe, Marcela Silvia
Metallothionein
Arcticchar
Estrogen
Polychlorinated Biphenyls
Toxicology
title_short Arctic char ( Salvelinus alpinus ) metallothionein: cDNA sequence, expression, and tissue-specific inhibition of cadmium-mediated metallothionein induction by 17β-estradiol, 4-OH-PCB 30, and PCB 104
title_full Arctic char ( Salvelinus alpinus ) metallothionein: cDNA sequence, expression, and tissue-specific inhibition of cadmium-mediated metallothionein induction by 17β-estradiol, 4-OH-PCB 30, and PCB 104
title_fullStr Arctic char ( Salvelinus alpinus ) metallothionein: cDNA sequence, expression, and tissue-specific inhibition of cadmium-mediated metallothionein induction by 17β-estradiol, 4-OH-PCB 30, and PCB 104
title_full_unstemmed Arctic char ( Salvelinus alpinus ) metallothionein: cDNA sequence, expression, and tissue-specific inhibition of cadmium-mediated metallothionein induction by 17β-estradiol, 4-OH-PCB 30, and PCB 104
title_sort Arctic char ( Salvelinus alpinus ) metallothionein: cDNA sequence, expression, and tissue-specific inhibition of cadmium-mediated metallothionein induction by 17β-estradiol, 4-OH-PCB 30, and PCB 104
dc.creator.none.fl_str_mv Gerpe, Marcela Silvia
Kling, Peter
Berg, Axel Håkan
Olsson, Per Erik
author Gerpe, Marcela Silvia
author_facet Gerpe, Marcela Silvia
Kling, Peter
Berg, Axel Håkan
Olsson, Per Erik
author_role author
author2 Kling, Peter
Berg, Axel Håkan
Olsson, Per Erik
author2_role author
author
author
dc.subject.none.fl_str_mv Metallothionein
Arcticchar
Estrogen
Polychlorinated Biphenyls
Toxicology
topic Metallothionein
Arcticchar
Estrogen
Polychlorinated Biphenyls
Toxicology
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this study, we haveexamined the basallevel expression and tissue‐specificexpressionpatternsof metallothionein (MT) in Arctic char following metal and E2 (17β‐estradiol) treatment. To study the gene regulation in Arctic char, the two MT isoforms were isolated from a lambda‐ZAP hepatic cDNA library and characterized. Determination of basal MT mRNA and MT expression for 10 different tissues revealed a lack of correlation between MT mRNA and MT levels. The inducibility of MT mRNA and the correlation to resulting MT levels were then determined for liver and kidney. We found a more rapid and stronger induction of MT mRNA in liver than in kidney at day 1 and 3 postinjection, whereas the MT protein quantification showed higher MT levels in kidney than in liver at days 3 and 7 postinjection. These discrepancies indicate that differences in metal handling or posttran‐scriptional regulation of MT exists between tissues. Whereas metals induce MT synthesis, E2 inhibit the hepatic MT expression. To examine the tissue specificity of this inhibition, we determined the effect of 17pj‐estradiol (E2) and two estrogenic PCBs (4′‐OH‐PCB 30 and PCB 104) on Cd‐mediated MT induction in liver and kidney. Although E2 and the estrogenic PCBs inhibited cadmium‐mediated hepatic MT induction, these compounds did not interfere with renal MT induction.
Fil: Gerpe, Marcela Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Kling, Peter. Umeå University; Suecia
Fil: Berg, Axel Håkan. Umeå University; Suecia
Fil: Olsson, Per Erik. Umeå University; Suecia
description In this study, we haveexamined the basallevel expression and tissue‐specificexpressionpatternsof metallothionein (MT) in Arctic char following metal and E2 (17β‐estradiol) treatment. To study the gene regulation in Arctic char, the two MT isoforms were isolated from a lambda‐ZAP hepatic cDNA library and characterized. Determination of basal MT mRNA and MT expression for 10 different tissues revealed a lack of correlation between MT mRNA and MT levels. The inducibility of MT mRNA and the correlation to resulting MT levels were then determined for liver and kidney. We found a more rapid and stronger induction of MT mRNA in liver than in kidney at day 1 and 3 postinjection, whereas the MT protein quantification showed higher MT levels in kidney than in liver at days 3 and 7 postinjection. These discrepancies indicate that differences in metal handling or posttran‐scriptional regulation of MT exists between tissues. Whereas metals induce MT synthesis, E2 inhibit the hepatic MT expression. To examine the tissue specificity of this inhibition, we determined the effect of 17pj‐estradiol (E2) and two estrogenic PCBs (4′‐OH‐PCB 30 and PCB 104) on Cd‐mediated MT induction in liver and kidney. Although E2 and the estrogenic PCBs inhibited cadmium‐mediated hepatic MT induction, these compounds did not interfere with renal MT induction.
publishDate 2000
dc.date.none.fl_str_mv 2000-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/69163
Gerpe, Marcela Silvia; Kling, Peter; Berg, Axel Håkan; Olsson, Per Erik; Arctic char ( Salvelinus alpinus ) metallothionein: cDNA sequence, expression, and tissue-specific inhibition of cadmium-mediated metallothionein induction by 17β-estradiol, 4-OH-PCB 30, and PCB 104; Society of Environmental Toxicology and Chemistry; Environmental Toxicology and Chemistry; 19; 3; 3-2000; 638-645
0730-7268
CONICET Digital
CONICET
url http://hdl.handle.net/11336/69163
identifier_str_mv Gerpe, Marcela Silvia; Kling, Peter; Berg, Axel Håkan; Olsson, Per Erik; Arctic char ( Salvelinus alpinus ) metallothionein: cDNA sequence, expression, and tissue-specific inhibition of cadmium-mediated metallothionein induction by 17β-estradiol, 4-OH-PCB 30, and PCB 104; Society of Environmental Toxicology and Chemistry; Environmental Toxicology and Chemistry; 19; 3; 3-2000; 638-645
0730-7268
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/etc.5620190316
info:eu-repo/semantics/altIdentifier/url/https://setac.onlinelibrary.wiley.com/doi/abs/10.1002/etc.5620190316
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society of Environmental Toxicology and Chemistry
publisher.none.fl_str_mv Society of Environmental Toxicology and Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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