Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements

Autores
Londonio, Juan Agustín; Hasuoka, Paul Emir; Pacheco, Pablo Hugo; Gil, Raul Andres; Smichowski, Patricia Nora
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A hyphenated system was developed for Hg(ii) and methylmercury (MeHg) preconcentration and speciation analysis by the online coupling of solid phase extraction (SPE), high performance liquid chromatography (HPLC) and inductively coupled plasma mass spectrometry (ICP-MS). Both analytes, Hg(ii) and MeHg, were preconcentrated on a microcolumn filled with multiwalled carbon nanotubes (MWCNTs) functionalised with poly-l-methionine (polymet-MWCNTs). During the method development the sorbent material was carefully studied and the solid phase extraction conditions were optimised. An enrichment factor of 190 for Hg(ii) and MeHg was obtained when 20 mL of sample was passed through the microcolumn. For the chromatographic separation, a mobile phase composed of a ternary mixture of 0.5% formic acid + 0.2% 2-mercaptoethanol + 20% methanol was used. Separation of both mercurial species was accomplished in ∼10 min on a 250 mm C18 column. The detection limits of the SPE-HPLC-ICP-MS method were 15 ng L−1 for Hg(ii) and 17 ng L−1 for MeHg. The relative standard deviations of peak area for 5 ng L−1 of each Hg species were below 5%. Recoveries of Hg(ii) and MeHg were never less than 93%. For checking the accuracy, BCR 643-tuna fish and TORT-3 lobster hepatopancreas certificate reference materials were analysed. Mercury species were determined in spiked fish oil-based dietary supplements and Antarctic water.
Fil: Londonio, Juan Agustín. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín. Instituto de Investigación en Ingeniería Ambiental; Argentina
Fil: Hasuoka, Paul Emir. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Pacheco, Pablo Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Gil, Raul Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Smichowski, Patricia Nora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Materia
Mercury speciation
carbon nanotubes
flow injection analysis
LC-ICPMS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/87358
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/87358
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplementsLondonio, Juan AgustínHasuoka, Paul EmirPacheco, Pablo HugoGil, Raul AndresSmichowski, Patricia NoraMercury speciationcarbon nanotubesflow injection analysisLC-ICPMShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A hyphenated system was developed for Hg(ii) and methylmercury (MeHg) preconcentration and speciation analysis by the online coupling of solid phase extraction (SPE), high performance liquid chromatography (HPLC) and inductively coupled plasma mass spectrometry (ICP-MS). Both analytes, Hg(ii) and MeHg, were preconcentrated on a microcolumn filled with multiwalled carbon nanotubes (MWCNTs) functionalised with poly-l-methionine (polymet-MWCNTs). During the method development the sorbent material was carefully studied and the solid phase extraction conditions were optimised. An enrichment factor of 190 for Hg(ii) and MeHg was obtained when 20 mL of sample was passed through the microcolumn. For the chromatographic separation, a mobile phase composed of a ternary mixture of 0.5% formic acid + 0.2% 2-mercaptoethanol + 20% methanol was used. Separation of both mercurial species was accomplished in ∼10 min on a 250 mm C18 column. The detection limits of the SPE-HPLC-ICP-MS method were 15 ng L−1 for Hg(ii) and 17 ng L−1 for MeHg. The relative standard deviations of peak area for 5 ng L−1 of each Hg species were below 5%. Recoveries of Hg(ii) and MeHg were never less than 93%. For checking the accuracy, BCR 643-tuna fish and TORT-3 lobster hepatopancreas certificate reference materials were analysed. Mercury species were determined in spiked fish oil-based dietary supplements and Antarctic water.Fil: Londonio, Juan Agustín. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín. Instituto de Investigación en Ingeniería Ambiental; ArgentinaFil: Hasuoka, Paul Emir. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Pacheco, Pablo Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Gil, Raul Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Smichowski, Patricia Nora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; ArgentinaRoyal Society of Chemistry2018-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/87358Londonio, Juan Agustín; Hasuoka, Paul Emir; Pacheco, Pablo Hugo; Gil, Raul Andres; Smichowski, Patricia Nora; Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements; Royal Society of Chemistry; Journal of Analytical Atomic Spectrometry; 33; 10; 10-2018; 1737-17440267-9477CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2018/ja/c8ja00188j#!divAbstractinfo:eu-repo/semantics/altIdentifier/doi/10.1039/C8JA00188Jinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:15:29Zoai:ri.conicet.gov.ar:11336/87358instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:15:29.837CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements
title Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements
spellingShingle Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements
Londonio, Juan Agustín
Mercury speciation
carbon nanotubes
flow injection analysis
LC-ICPMS
title_short Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements
title_full Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements
title_fullStr Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements
title_full_unstemmed Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements
title_sort Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements
dc.creator.none.fl_str_mv Londonio, Juan Agustín
Hasuoka, Paul Emir
Pacheco, Pablo Hugo
Gil, Raul Andres
Smichowski, Patricia Nora
author Londonio, Juan Agustín
author_facet Londonio, Juan Agustín
Hasuoka, Paul Emir
Pacheco, Pablo Hugo
Gil, Raul Andres
Smichowski, Patricia Nora
author_role author
author2 Hasuoka, Paul Emir
Pacheco, Pablo Hugo
Gil, Raul Andres
Smichowski, Patricia Nora
author2_role author
author
author
author
dc.subject.none.fl_str_mv Mercury speciation
carbon nanotubes
flow injection analysis
LC-ICPMS
topic Mercury speciation
carbon nanotubes
flow injection analysis
LC-ICPMS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A hyphenated system was developed for Hg(ii) and methylmercury (MeHg) preconcentration and speciation analysis by the online coupling of solid phase extraction (SPE), high performance liquid chromatography (HPLC) and inductively coupled plasma mass spectrometry (ICP-MS). Both analytes, Hg(ii) and MeHg, were preconcentrated on a microcolumn filled with multiwalled carbon nanotubes (MWCNTs) functionalised with poly-l-methionine (polymet-MWCNTs). During the method development the sorbent material was carefully studied and the solid phase extraction conditions were optimised. An enrichment factor of 190 for Hg(ii) and MeHg was obtained when 20 mL of sample was passed through the microcolumn. For the chromatographic separation, a mobile phase composed of a ternary mixture of 0.5% formic acid + 0.2% 2-mercaptoethanol + 20% methanol was used. Separation of both mercurial species was accomplished in ∼10 min on a 250 mm C18 column. The detection limits of the SPE-HPLC-ICP-MS method were 15 ng L−1 for Hg(ii) and 17 ng L−1 for MeHg. The relative standard deviations of peak area for 5 ng L−1 of each Hg species were below 5%. Recoveries of Hg(ii) and MeHg were never less than 93%. For checking the accuracy, BCR 643-tuna fish and TORT-3 lobster hepatopancreas certificate reference materials were analysed. Mercury species were determined in spiked fish oil-based dietary supplements and Antarctic water.
Fil: Londonio, Juan Agustín. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín. Instituto de Investigación en Ingeniería Ambiental; Argentina
Fil: Hasuoka, Paul Emir. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Pacheco, Pablo Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Gil, Raul Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Smichowski, Patricia Nora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
description A hyphenated system was developed for Hg(ii) and methylmercury (MeHg) preconcentration and speciation analysis by the online coupling of solid phase extraction (SPE), high performance liquid chromatography (HPLC) and inductively coupled plasma mass spectrometry (ICP-MS). Both analytes, Hg(ii) and MeHg, were preconcentrated on a microcolumn filled with multiwalled carbon nanotubes (MWCNTs) functionalised with poly-l-methionine (polymet-MWCNTs). During the method development the sorbent material was carefully studied and the solid phase extraction conditions were optimised. An enrichment factor of 190 for Hg(ii) and MeHg was obtained when 20 mL of sample was passed through the microcolumn. For the chromatographic separation, a mobile phase composed of a ternary mixture of 0.5% formic acid + 0.2% 2-mercaptoethanol + 20% methanol was used. Separation of both mercurial species was accomplished in ∼10 min on a 250 mm C18 column. The detection limits of the SPE-HPLC-ICP-MS method were 15 ng L−1 for Hg(ii) and 17 ng L−1 for MeHg. The relative standard deviations of peak area for 5 ng L−1 of each Hg species were below 5%. Recoveries of Hg(ii) and MeHg were never less than 93%. For checking the accuracy, BCR 643-tuna fish and TORT-3 lobster hepatopancreas certificate reference materials were analysed. Mercury species were determined in spiked fish oil-based dietary supplements and Antarctic water.
publishDate 2018
dc.date.none.fl_str_mv 2018-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/87358
Londonio, Juan Agustín; Hasuoka, Paul Emir; Pacheco, Pablo Hugo; Gil, Raul Andres; Smichowski, Patricia Nora; Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements; Royal Society of Chemistry; Journal of Analytical Atomic Spectrometry; 33; 10; 10-2018; 1737-1744
0267-9477
CONICET Digital
CONICET
url http://hdl.handle.net/11336/87358
identifier_str_mv Londonio, Juan Agustín; Hasuoka, Paul Emir; Pacheco, Pablo Hugo; Gil, Raul Andres; Smichowski, Patricia Nora; Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements; Royal Society of Chemistry; Journal of Analytical Atomic Spectrometry; 33; 10; 10-2018; 1737-1744
0267-9477
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2018/ja/c8ja00188j#!divAbstract
info:eu-repo/semantics/altIdentifier/doi/10.1039/C8JA00188J
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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