Functional conservation of clock output signaling between flies and intertidal crabs

Autores
Beckwith, Esteban Javier; Lelito, Katherine R.; Hsu, Yun Wei A.; Medina, Billie M.; Shafer, Orie; Ceriani, Maria Fernanda; de la Iglesia, Horacio O
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Intertidal species have both circadian and circatidal clocks. Although the behavioral evidence for these oscillators is more than 5 decades old, virtually nothing is known about their molecular clockwork. Pigment-dispersing hormones (PDHs) were originally described in crustaceans. Their insect homologs, pigment-dispersing factors (PDFs), have a prominent role as clock output and synchronizing signals released from clock neurons. We show that gene duplication in crabs has led to two PDH genes (β-pdh-I and β-pdh-II). Phylogenetically, β-pdh-I is more closely related to insect pdf than to β-pdh-II, and we hypothesized that β-PDH-I may represent a canonical clock output signal. Accordingly, β-PDH-I expression in the brain of the intertidal crab Cancer productus is similar to that of PDF in Drosophila melanogaster, and neurons that express PDH-I also show CYCLE-like immunoreactivity. Using D. melanogaster pdf-null mutants (pdf(01)) as a heterologous system, we show that β-pdh-I is indistinguishable from pdf in its ability to rescue the mutant arrhythmic phenotype, but β-pdh-II fails to restore the wild-type phenotype. Application of the three peptides to explanted brains shows that PDF and β-PDH-I are equally effective in inducing the signal transduction cascade of the PDF receptor, but β-PDH-II fails to induce a normal cascade. Our results represent the first functional characterization of a putative molecular clock output in an intertidal species and may provide a critical step towards the characterization of molecular components of biological clocks in intertidal organisms.
Fil: Beckwith, Esteban Javier. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Lelito, Katherine R.. University Of Michigan; Estados Unidos
Fil: Hsu, Yun Wei A.. University Of Washington; Estados Unidos
Fil: Medina, Billie M.. University Of Washington; Estados Unidos
Fil: Shafer, Orie. University Of Michigan; Estados Unidos
Fil: Ceriani, Maria Fernanda. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: de la Iglesia, Horacio O. University Of Washington; Estados Unidos
Materia
Pdh
Pdf
Circatidal Clocks,
Intertidal Species
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/12810
Acceder
id CONICETDig_767d6c344ba1354645a77b702f01fc91
oai_identifier_str oai:ri.conicet.gov.ar:11336/12810
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Functional conservation of clock output signaling between flies and intertidal crabsBeckwith, Esteban JavierLelito, Katherine R.Hsu, Yun Wei A.Medina, Billie M.Shafer, OrieCeriani, Maria Fernandade la Iglesia, Horacio OPdhPdfCircatidal Clocks,Intertidal Specieshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Intertidal species have both circadian and circatidal clocks. Although the behavioral evidence for these oscillators is more than 5 decades old, virtually nothing is known about their molecular clockwork. Pigment-dispersing hormones (PDHs) were originally described in crustaceans. Their insect homologs, pigment-dispersing factors (PDFs), have a prominent role as clock output and synchronizing signals released from clock neurons. We show that gene duplication in crabs has led to two PDH genes (β-pdh-I and β-pdh-II). Phylogenetically, β-pdh-I is more closely related to insect pdf than to β-pdh-II, and we hypothesized that β-PDH-I may represent a canonical clock output signal. Accordingly, β-PDH-I expression in the brain of the intertidal crab Cancer productus is similar to that of PDF in Drosophila melanogaster, and neurons that express PDH-I also show CYCLE-like immunoreactivity. Using D. melanogaster pdf-null mutants (pdf(01)) as a heterologous system, we show that β-pdh-I is indistinguishable from pdf in its ability to rescue the mutant arrhythmic phenotype, but β-pdh-II fails to restore the wild-type phenotype. Application of the three peptides to explanted brains shows that PDF and β-PDH-I are equally effective in inducing the signal transduction cascade of the PDF receptor, but β-PDH-II fails to induce a normal cascade. Our results represent the first functional characterization of a putative molecular clock output in an intertidal species and may provide a critical step towards the characterization of molecular components of biological clocks in intertidal organisms.Fil: Beckwith, Esteban Javier. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; ArgentinaFil: Lelito, Katherine R.. University Of Michigan; Estados UnidosFil: Hsu, Yun Wei A.. University Of Washington; Estados UnidosFil: Medina, Billie M.. University Of Washington; Estados UnidosFil: Shafer, Orie. University Of Michigan; Estados UnidosFil: Ceriani, Maria Fernanda. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; ArgentinaFil: de la Iglesia, Horacio O. University Of Washington; Estados UnidosSage Publications2011-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/12810Beckwith, Esteban Javier; Lelito, Katherine R.; Hsu, Yun Wei A.; Medina, Billie M.; Shafer, Orie; et al.; Functional conservation of clock output signaling between flies and intertidal crabs; Sage Publications; Journal Of Biological Rhythms.; 26; 6; 12-2011; 518-5290748-7304enginfo:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/pdf/10.1177/0748730411420242info:eu-repo/semantics/altIdentifier/doi/10.1177/0748730411420242info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:50Zoai:ri.conicet.gov.ar:11336/12810instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:51.095CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Functional conservation of clock output signaling between flies and intertidal crabs
title Functional conservation of clock output signaling between flies and intertidal crabs
spellingShingle Functional conservation of clock output signaling between flies and intertidal crabs
Beckwith, Esteban Javier
Pdh
Pdf
Circatidal Clocks,
Intertidal Species
title_short Functional conservation of clock output signaling between flies and intertidal crabs
title_full Functional conservation of clock output signaling between flies and intertidal crabs
title_fullStr Functional conservation of clock output signaling between flies and intertidal crabs
title_full_unstemmed Functional conservation of clock output signaling between flies and intertidal crabs
title_sort Functional conservation of clock output signaling between flies and intertidal crabs
dc.creator.none.fl_str_mv Beckwith, Esteban Javier
Lelito, Katherine R.
Hsu, Yun Wei A.
Medina, Billie M.
Shafer, Orie
Ceriani, Maria Fernanda
de la Iglesia, Horacio O
author Beckwith, Esteban Javier
author_facet Beckwith, Esteban Javier
Lelito, Katherine R.
Hsu, Yun Wei A.
Medina, Billie M.
Shafer, Orie
Ceriani, Maria Fernanda
de la Iglesia, Horacio O
author_role author
author2 Lelito, Katherine R.
Hsu, Yun Wei A.
Medina, Billie M.
Shafer, Orie
Ceriani, Maria Fernanda
de la Iglesia, Horacio O
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Pdh
Pdf
Circatidal Clocks,
Intertidal Species
topic Pdh
Pdf
Circatidal Clocks,
Intertidal Species
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Intertidal species have both circadian and circatidal clocks. Although the behavioral evidence for these oscillators is more than 5 decades old, virtually nothing is known about their molecular clockwork. Pigment-dispersing hormones (PDHs) were originally described in crustaceans. Their insect homologs, pigment-dispersing factors (PDFs), have a prominent role as clock output and synchronizing signals released from clock neurons. We show that gene duplication in crabs has led to two PDH genes (β-pdh-I and β-pdh-II). Phylogenetically, β-pdh-I is more closely related to insect pdf than to β-pdh-II, and we hypothesized that β-PDH-I may represent a canonical clock output signal. Accordingly, β-PDH-I expression in the brain of the intertidal crab Cancer productus is similar to that of PDF in Drosophila melanogaster, and neurons that express PDH-I also show CYCLE-like immunoreactivity. Using D. melanogaster pdf-null mutants (pdf(01)) as a heterologous system, we show that β-pdh-I is indistinguishable from pdf in its ability to rescue the mutant arrhythmic phenotype, but β-pdh-II fails to restore the wild-type phenotype. Application of the three peptides to explanted brains shows that PDF and β-PDH-I are equally effective in inducing the signal transduction cascade of the PDF receptor, but β-PDH-II fails to induce a normal cascade. Our results represent the first functional characterization of a putative molecular clock output in an intertidal species and may provide a critical step towards the characterization of molecular components of biological clocks in intertidal organisms.
Fil: Beckwith, Esteban Javier. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Lelito, Katherine R.. University Of Michigan; Estados Unidos
Fil: Hsu, Yun Wei A.. University Of Washington; Estados Unidos
Fil: Medina, Billie M.. University Of Washington; Estados Unidos
Fil: Shafer, Orie. University Of Michigan; Estados Unidos
Fil: Ceriani, Maria Fernanda. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: de la Iglesia, Horacio O. University Of Washington; Estados Unidos
description Intertidal species have both circadian and circatidal clocks. Although the behavioral evidence for these oscillators is more than 5 decades old, virtually nothing is known about their molecular clockwork. Pigment-dispersing hormones (PDHs) were originally described in crustaceans. Their insect homologs, pigment-dispersing factors (PDFs), have a prominent role as clock output and synchronizing signals released from clock neurons. We show that gene duplication in crabs has led to two PDH genes (β-pdh-I and β-pdh-II). Phylogenetically, β-pdh-I is more closely related to insect pdf than to β-pdh-II, and we hypothesized that β-PDH-I may represent a canonical clock output signal. Accordingly, β-PDH-I expression in the brain of the intertidal crab Cancer productus is similar to that of PDF in Drosophila melanogaster, and neurons that express PDH-I also show CYCLE-like immunoreactivity. Using D. melanogaster pdf-null mutants (pdf(01)) as a heterologous system, we show that β-pdh-I is indistinguishable from pdf in its ability to rescue the mutant arrhythmic phenotype, but β-pdh-II fails to restore the wild-type phenotype. Application of the three peptides to explanted brains shows that PDF and β-PDH-I are equally effective in inducing the signal transduction cascade of the PDF receptor, but β-PDH-II fails to induce a normal cascade. Our results represent the first functional characterization of a putative molecular clock output in an intertidal species and may provide a critical step towards the characterization of molecular components of biological clocks in intertidal organisms.
publishDate 2011
dc.date.none.fl_str_mv 2011-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/12810
Beckwith, Esteban Javier; Lelito, Katherine R.; Hsu, Yun Wei A.; Medina, Billie M.; Shafer, Orie; et al.; Functional conservation of clock output signaling between flies and intertidal crabs; Sage Publications; Journal Of Biological Rhythms.; 26; 6; 12-2011; 518-529
0748-7304
url http://hdl.handle.net/11336/12810
identifier_str_mv Beckwith, Esteban Javier; Lelito, Katherine R.; Hsu, Yun Wei A.; Medina, Billie M.; Shafer, Orie; et al.; Functional conservation of clock output signaling between flies and intertidal crabs; Sage Publications; Journal Of Biological Rhythms.; 26; 6; 12-2011; 518-529
0748-7304
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/pdf/10.1177/0748730411420242
info:eu-repo/semantics/altIdentifier/doi/10.1177/0748730411420242
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sage Publications
publisher.none.fl_str_mv Sage Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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