Antibacterial activity of medicinal plants against Streptococcus agalactiae

Autores
Novosak, Marina Gisel; Winnik, Daniana Lilian; Laczeski, Margarita Ester; Quiroga, Marina Inés
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Streptococcus agalactiae, group B Streptococcus (GBS), infects and causes severe diseases in humans and numerous animal species, including fish, given its ability to cross the host-specific barrier. The emergence of antibiotic resistant GBS strains makes it necessary to look for alternatives to treat and prevent infections that it produces. The aim of the present study was to determine the antibacterial activity of ethanolic and aqueous extracts of medicinal plants from Misiones province, northeast Argentina, against GBS from humans and fish. We used human Streptococcus agalactiae ATCC® BAA-611™ and tilapia Streptococcus agalactiae ATCC® 51487™ strains. Minimum Inhibitory Dose (MID) was determined by the disc diffusion method. Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), MBC/MIC ratio, drug synergism with commercial antibiotics, and resistance assays were determined with extracts that showed antibacterial activity. Medium Lethal Dose 50 (LD50) was determined by the Artemia salina assay. For ethanolic and aqueous Eugenia uniflora L. extracts, we obtained a MID = 0.5 mg.disc-1. For both extracts of Eugenia uniflora L., the MIC and MBC values were 1 mg.mL-1 and 5 mg.mL-1, respectively. The MICI (MBC/MIC ratio) = 5 qualified the action of these extracts as bacteriostatic. The drug synergism assay with ampicillin, erythromycin, and clindamycin combination and extracts showed indifference. The LD50 of the aqueous extract was 0.82 mg.mL-1 indicating moderate toxicity. This work is a first step to identify chemical compounds in native medicinal plants of Misiones, Argentina, that could mean an alternative for the treatment of Streptococcus agalactiae infections.
Fil: Novosak, Marina Gisel. Universidad Nacional de Misiones; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Winnik, Daniana Lilian. Universidad Nacional de Misiones; Argentina
Fil: Laczeski, Margarita Ester. Universidad Nacional de Misiones; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Quiroga, Marina Inés. Universidad Nacional de Misiones; Argentina
Materia
AQUEOUS EXTRACT
BACTERIAL
DRUG RESISTANCE
ETHANOLIC EXTRACT
MEDICINAL
PLANTS
STREPTOCOCCUS AGALACTIAE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/222137
Acceder
id CONICETDig_764bf42624b67f04e5b29f6498644295
oai_identifier_str oai:ri.conicet.gov.ar:11336/222137
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antibacterial activity of medicinal plants against Streptococcus agalactiaeNovosak, Marina GiselWinnik, Daniana LilianLaczeski, Margarita EsterQuiroga, Marina InésAQUEOUS EXTRACTBACTERIALDRUG RESISTANCEETHANOLIC EXTRACTMEDICINALPLANTSSTREPTOCOCCUS AGALACTIAEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Streptococcus agalactiae, group B Streptococcus (GBS), infects and causes severe diseases in humans and numerous animal species, including fish, given its ability to cross the host-specific barrier. The emergence of antibiotic resistant GBS strains makes it necessary to look for alternatives to treat and prevent infections that it produces. The aim of the present study was to determine the antibacterial activity of ethanolic and aqueous extracts of medicinal plants from Misiones province, northeast Argentina, against GBS from humans and fish. We used human Streptococcus agalactiae ATCC® BAA-611™ and tilapia Streptococcus agalactiae ATCC® 51487™ strains. Minimum Inhibitory Dose (MID) was determined by the disc diffusion method. Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), MBC/MIC ratio, drug synergism with commercial antibiotics, and resistance assays were determined with extracts that showed antibacterial activity. Medium Lethal Dose 50 (LD50) was determined by the Artemia salina assay. For ethanolic and aqueous Eugenia uniflora L. extracts, we obtained a MID = 0.5 mg.disc-1. For both extracts of Eugenia uniflora L., the MIC and MBC values were 1 mg.mL-1 and 5 mg.mL-1, respectively. The MICI (MBC/MIC ratio) = 5 qualified the action of these extracts as bacteriostatic. The drug synergism assay with ampicillin, erythromycin, and clindamycin combination and extracts showed indifference. The LD50 of the aqueous extract was 0.82 mg.mL-1 indicating moderate toxicity. This work is a first step to identify chemical compounds in native medicinal plants of Misiones, Argentina, that could mean an alternative for the treatment of Streptococcus agalactiae infections.Fil: Novosak, Marina Gisel. Universidad Nacional de Misiones; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Winnik, Daniana Lilian. Universidad Nacional de Misiones; ArgentinaFil: Laczeski, Margarita Ester. Universidad Nacional de Misiones; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Quiroga, Marina Inés. Universidad Nacional de Misiones; ArgentinaSciCell s.r.o.2022-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/222137Novosak, Marina Gisel; Winnik, Daniana Lilian; Laczeski, Margarita Ester; Quiroga, Marina Inés; Antibacterial activity of medicinal plants against Streptococcus agalactiae; SciCell s.r.o.; Nova Biotechnologica et Chimica; 21; 2; 12-2022; 1-111338-69051339-004XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.scicell.org/index.php/NBC/article/view/1264/801info:eu-repo/semantics/altIdentifier/doi/10.36547/nbc.1264info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:49:25Zoai:ri.conicet.gov.ar:11336/222137instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:49:25.892CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antibacterial activity of medicinal plants against Streptococcus agalactiae
title Antibacterial activity of medicinal plants against Streptococcus agalactiae
spellingShingle Antibacterial activity of medicinal plants against Streptococcus agalactiae
Novosak, Marina Gisel
AQUEOUS EXTRACT
BACTERIAL
DRUG RESISTANCE
ETHANOLIC EXTRACT
MEDICINAL
PLANTS
STREPTOCOCCUS AGALACTIAE
title_short Antibacterial activity of medicinal plants against Streptococcus agalactiae
title_full Antibacterial activity of medicinal plants against Streptococcus agalactiae
title_fullStr Antibacterial activity of medicinal plants against Streptococcus agalactiae
title_full_unstemmed Antibacterial activity of medicinal plants against Streptococcus agalactiae
title_sort Antibacterial activity of medicinal plants against Streptococcus agalactiae
dc.creator.none.fl_str_mv Novosak, Marina Gisel
Winnik, Daniana Lilian
Laczeski, Margarita Ester
Quiroga, Marina Inés
author Novosak, Marina Gisel
author_facet Novosak, Marina Gisel
Winnik, Daniana Lilian
Laczeski, Margarita Ester
Quiroga, Marina Inés
author_role author
author2 Winnik, Daniana Lilian
Laczeski, Margarita Ester
Quiroga, Marina Inés
author2_role author
author
author
dc.subject.none.fl_str_mv AQUEOUS EXTRACT
BACTERIAL
DRUG RESISTANCE
ETHANOLIC EXTRACT
MEDICINAL
PLANTS
STREPTOCOCCUS AGALACTIAE
topic AQUEOUS EXTRACT
BACTERIAL
DRUG RESISTANCE
ETHANOLIC EXTRACT
MEDICINAL
PLANTS
STREPTOCOCCUS AGALACTIAE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Streptococcus agalactiae, group B Streptococcus (GBS), infects and causes severe diseases in humans and numerous animal species, including fish, given its ability to cross the host-specific barrier. The emergence of antibiotic resistant GBS strains makes it necessary to look for alternatives to treat and prevent infections that it produces. The aim of the present study was to determine the antibacterial activity of ethanolic and aqueous extracts of medicinal plants from Misiones province, northeast Argentina, against GBS from humans and fish. We used human Streptococcus agalactiae ATCC® BAA-611™ and tilapia Streptococcus agalactiae ATCC® 51487™ strains. Minimum Inhibitory Dose (MID) was determined by the disc diffusion method. Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), MBC/MIC ratio, drug synergism with commercial antibiotics, and resistance assays were determined with extracts that showed antibacterial activity. Medium Lethal Dose 50 (LD50) was determined by the Artemia salina assay. For ethanolic and aqueous Eugenia uniflora L. extracts, we obtained a MID = 0.5 mg.disc-1. For both extracts of Eugenia uniflora L., the MIC and MBC values were 1 mg.mL-1 and 5 mg.mL-1, respectively. The MICI (MBC/MIC ratio) = 5 qualified the action of these extracts as bacteriostatic. The drug synergism assay with ampicillin, erythromycin, and clindamycin combination and extracts showed indifference. The LD50 of the aqueous extract was 0.82 mg.mL-1 indicating moderate toxicity. This work is a first step to identify chemical compounds in native medicinal plants of Misiones, Argentina, that could mean an alternative for the treatment of Streptococcus agalactiae infections.
Fil: Novosak, Marina Gisel. Universidad Nacional de Misiones; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Winnik, Daniana Lilian. Universidad Nacional de Misiones; Argentina
Fil: Laczeski, Margarita Ester. Universidad Nacional de Misiones; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Quiroga, Marina Inés. Universidad Nacional de Misiones; Argentina
description Streptococcus agalactiae, group B Streptococcus (GBS), infects and causes severe diseases in humans and numerous animal species, including fish, given its ability to cross the host-specific barrier. The emergence of antibiotic resistant GBS strains makes it necessary to look for alternatives to treat and prevent infections that it produces. The aim of the present study was to determine the antibacterial activity of ethanolic and aqueous extracts of medicinal plants from Misiones province, northeast Argentina, against GBS from humans and fish. We used human Streptococcus agalactiae ATCC® BAA-611™ and tilapia Streptococcus agalactiae ATCC® 51487™ strains. Minimum Inhibitory Dose (MID) was determined by the disc diffusion method. Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), MBC/MIC ratio, drug synergism with commercial antibiotics, and resistance assays were determined with extracts that showed antibacterial activity. Medium Lethal Dose 50 (LD50) was determined by the Artemia salina assay. For ethanolic and aqueous Eugenia uniflora L. extracts, we obtained a MID = 0.5 mg.disc-1. For both extracts of Eugenia uniflora L., the MIC and MBC values were 1 mg.mL-1 and 5 mg.mL-1, respectively. The MICI (MBC/MIC ratio) = 5 qualified the action of these extracts as bacteriostatic. The drug synergism assay with ampicillin, erythromycin, and clindamycin combination and extracts showed indifference. The LD50 of the aqueous extract was 0.82 mg.mL-1 indicating moderate toxicity. This work is a first step to identify chemical compounds in native medicinal plants of Misiones, Argentina, that could mean an alternative for the treatment of Streptococcus agalactiae infections.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/222137
Novosak, Marina Gisel; Winnik, Daniana Lilian; Laczeski, Margarita Ester; Quiroga, Marina Inés; Antibacterial activity of medicinal plants against Streptococcus agalactiae; SciCell s.r.o.; Nova Biotechnologica et Chimica; 21; 2; 12-2022; 1-11
1338-6905
1339-004X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/222137
identifier_str_mv Novosak, Marina Gisel; Winnik, Daniana Lilian; Laczeski, Margarita Ester; Quiroga, Marina Inés; Antibacterial activity of medicinal plants against Streptococcus agalactiae; SciCell s.r.o.; Nova Biotechnologica et Chimica; 21; 2; 12-2022; 1-11
1338-6905
1339-004X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.scicell.org/index.php/NBC/article/view/1264/801
info:eu-repo/semantics/altIdentifier/doi/10.36547/nbc.1264
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv SciCell s.r.o.
publisher.none.fl_str_mv SciCell s.r.o.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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