Fluoroquinolone-resistant streptococcus agalactiae invasive isolates recovered in Argentina

Autores
Arias, Bárbara Soledad; Kovacec, Verónica Ivana; Vigliarolo, Laura; Suárez, Mariana; Tersigni, Carina Laura; Müller, Loana; Lopardo, Horacio Angel; Bonofiglio, Laura; Mollerach, Marta Eugenia
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Streptococcus agalactiae or group B Streptococcus (GBS) is an important pathogen in neonates and nonpregnant individuals. Epidemiological studies of GBS resistance to fluoroquinolones (FQs) in Latin America are scarce. This study aimed to determine the local prevalence of FQ resistance in the frame of a national, prospective multicenter study of invasive GBS infections and to investigate mechanisms of resistance, serotype distribution, and clonal relationships among resistant isolates. Methods: From July 2014 to July 2015, 162 invasive GBS isolates were collected from 86 health care centers in 32 Argentinean cities. All isolates were screened for FQ nonsusceptibility using a five-disc scheme: Levofloxacin (LVX), ciprofloxacin, norfloxacin (NOR), ofloxacin, and pefloxacin (PF). LVX minimal inhibitory concentration (MIC) was determined by the agar dilution method. Sequencing of internal regions of gyrA and parC genes was performed. Capsular typing and genetic characterization of nonsusceptible isolates were assessed by latex agglutination, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing. Results: Twenty-four of one hundred sixty-two GBS isolates exhibited no inhibition zones to all tested FQs with an MIC range of 16-32 mg/L for LVX, and one isolate with MIC = 1 mg/L showed no inhibition zones around NOR and PF discs. In all resistant isolates, point mutations were detected in both genes. Serotype Ib was prevalent (88%). One PFGE type accounted for 84% of the FQ-resistant isolates and belonged to serotype Ib, sequence type 10. Conclusions: The prevalence of FQ resistance was 14.8% likely to be associated with dissemination of an ST10/serotype Ib clone. The unexpected high rate of resistance emphasizes the relevance for continuous surveillance of GBS epidemiology and antibiotic susceptibility.
Fil: Arias, Bárbara Soledad. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Kovacec, Verónica Ivana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Vigliarolo, Laura. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Suárez, Mariana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Tersigni, Carina Laura. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Müller, Loana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Lopardo, Horacio Angel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Bonofiglio, Laura. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Mollerach, Marta Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Materia
ANTIBIOTIC RESISTANCE
EPIDEMIOLOGY
FLUOROQUINOLONES
STREPTOCOCCUS AGALACTIAE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/130190
Acceder
id CONICETDig_8d6de3b9cb3a3e2c1d6673c3c1018135
oai_identifier_str oai:ri.conicet.gov.ar:11336/130190
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fluoroquinolone-resistant streptococcus agalactiae invasive isolates recovered in ArgentinaArias, Bárbara SoledadKovacec, Verónica IvanaVigliarolo, LauraSuárez, MarianaTersigni, Carina LauraMüller, LoanaLopardo, Horacio AngelBonofiglio, LauraMollerach, Marta EugeniaANTIBIOTIC RESISTANCEEPIDEMIOLOGYFLUOROQUINOLONESSTREPTOCOCCUS AGALACTIAEhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Background: Streptococcus agalactiae or group B Streptococcus (GBS) is an important pathogen in neonates and nonpregnant individuals. Epidemiological studies of GBS resistance to fluoroquinolones (FQs) in Latin America are scarce. This study aimed to determine the local prevalence of FQ resistance in the frame of a national, prospective multicenter study of invasive GBS infections and to investigate mechanisms of resistance, serotype distribution, and clonal relationships among resistant isolates. Methods: From July 2014 to July 2015, 162 invasive GBS isolates were collected from 86 health care centers in 32 Argentinean cities. All isolates were screened for FQ nonsusceptibility using a five-disc scheme: Levofloxacin (LVX), ciprofloxacin, norfloxacin (NOR), ofloxacin, and pefloxacin (PF). LVX minimal inhibitory concentration (MIC) was determined by the agar dilution method. Sequencing of internal regions of gyrA and parC genes was performed. Capsular typing and genetic characterization of nonsusceptible isolates were assessed by latex agglutination, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing. Results: Twenty-four of one hundred sixty-two GBS isolates exhibited no inhibition zones to all tested FQs with an MIC range of 16-32 mg/L for LVX, and one isolate with MIC = 1 mg/L showed no inhibition zones around NOR and PF discs. In all resistant isolates, point mutations were detected in both genes. Serotype Ib was prevalent (88%). One PFGE type accounted for 84% of the FQ-resistant isolates and belonged to serotype Ib, sequence type 10. Conclusions: The prevalence of FQ resistance was 14.8% likely to be associated with dissemination of an ST10/serotype Ib clone. The unexpected high rate of resistance emphasizes the relevance for continuous surveillance of GBS epidemiology and antibiotic susceptibility.Fil: Arias, Bárbara Soledad. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Kovacec, Verónica Ivana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Vigliarolo, Laura. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Suárez, Mariana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Tersigni, Carina Laura. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Müller, Loana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Lopardo, Horacio Angel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Bonofiglio, Laura. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Mollerach, Marta Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaMary Ann Liebert2019-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/130190Arias, Bárbara Soledad; Kovacec, Verónica Ivana; Vigliarolo, Laura; Suárez, Mariana; Tersigni, Carina Laura; et al.; Fluoroquinolone-resistant streptococcus agalactiae invasive isolates recovered in Argentina; Mary Ann Liebert; Microbial Drug Resistance: Mechanisms Epidemiology and Disease; 25; 5; 6-2019; 739-7431076-6294CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1089/mdr.2018.0246info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:22:49Zoai:ri.conicet.gov.ar:11336/130190instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:22:49.974CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fluoroquinolone-resistant streptococcus agalactiae invasive isolates recovered in Argentina
title Fluoroquinolone-resistant streptococcus agalactiae invasive isolates recovered in Argentina
spellingShingle Fluoroquinolone-resistant streptococcus agalactiae invasive isolates recovered in Argentina
Arias, Bárbara Soledad
ANTIBIOTIC RESISTANCE
EPIDEMIOLOGY
FLUOROQUINOLONES
STREPTOCOCCUS AGALACTIAE
title_short Fluoroquinolone-resistant streptococcus agalactiae invasive isolates recovered in Argentina
title_full Fluoroquinolone-resistant streptococcus agalactiae invasive isolates recovered in Argentina
title_fullStr Fluoroquinolone-resistant streptococcus agalactiae invasive isolates recovered in Argentina
title_full_unstemmed Fluoroquinolone-resistant streptococcus agalactiae invasive isolates recovered in Argentina
title_sort Fluoroquinolone-resistant streptococcus agalactiae invasive isolates recovered in Argentina
dc.creator.none.fl_str_mv Arias, Bárbara Soledad
Kovacec, Verónica Ivana
Vigliarolo, Laura
Suárez, Mariana
Tersigni, Carina Laura
Müller, Loana
Lopardo, Horacio Angel
Bonofiglio, Laura
Mollerach, Marta Eugenia
author Arias, Bárbara Soledad
author_facet Arias, Bárbara Soledad
Kovacec, Verónica Ivana
Vigliarolo, Laura
Suárez, Mariana
Tersigni, Carina Laura
Müller, Loana
Lopardo, Horacio Angel
Bonofiglio, Laura
Mollerach, Marta Eugenia
author_role author
author2 Kovacec, Verónica Ivana
Vigliarolo, Laura
Suárez, Mariana
Tersigni, Carina Laura
Müller, Loana
Lopardo, Horacio Angel
Bonofiglio, Laura
Mollerach, Marta Eugenia
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIBIOTIC RESISTANCE
EPIDEMIOLOGY
FLUOROQUINOLONES
STREPTOCOCCUS AGALACTIAE
topic ANTIBIOTIC RESISTANCE
EPIDEMIOLOGY
FLUOROQUINOLONES
STREPTOCOCCUS AGALACTIAE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Streptococcus agalactiae or group B Streptococcus (GBS) is an important pathogen in neonates and nonpregnant individuals. Epidemiological studies of GBS resistance to fluoroquinolones (FQs) in Latin America are scarce. This study aimed to determine the local prevalence of FQ resistance in the frame of a national, prospective multicenter study of invasive GBS infections and to investigate mechanisms of resistance, serotype distribution, and clonal relationships among resistant isolates. Methods: From July 2014 to July 2015, 162 invasive GBS isolates were collected from 86 health care centers in 32 Argentinean cities. All isolates were screened for FQ nonsusceptibility using a five-disc scheme: Levofloxacin (LVX), ciprofloxacin, norfloxacin (NOR), ofloxacin, and pefloxacin (PF). LVX minimal inhibitory concentration (MIC) was determined by the agar dilution method. Sequencing of internal regions of gyrA and parC genes was performed. Capsular typing and genetic characterization of nonsusceptible isolates were assessed by latex agglutination, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing. Results: Twenty-four of one hundred sixty-two GBS isolates exhibited no inhibition zones to all tested FQs with an MIC range of 16-32 mg/L for LVX, and one isolate with MIC = 1 mg/L showed no inhibition zones around NOR and PF discs. In all resistant isolates, point mutations were detected in both genes. Serotype Ib was prevalent (88%). One PFGE type accounted for 84% of the FQ-resistant isolates and belonged to serotype Ib, sequence type 10. Conclusions: The prevalence of FQ resistance was 14.8% likely to be associated with dissemination of an ST10/serotype Ib clone. The unexpected high rate of resistance emphasizes the relevance for continuous surveillance of GBS epidemiology and antibiotic susceptibility.
Fil: Arias, Bárbara Soledad. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Kovacec, Verónica Ivana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Vigliarolo, Laura. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Suárez, Mariana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Tersigni, Carina Laura. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Müller, Loana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Lopardo, Horacio Angel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Bonofiglio, Laura. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Mollerach, Marta Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
description Background: Streptococcus agalactiae or group B Streptococcus (GBS) is an important pathogen in neonates and nonpregnant individuals. Epidemiological studies of GBS resistance to fluoroquinolones (FQs) in Latin America are scarce. This study aimed to determine the local prevalence of FQ resistance in the frame of a national, prospective multicenter study of invasive GBS infections and to investigate mechanisms of resistance, serotype distribution, and clonal relationships among resistant isolates. Methods: From July 2014 to July 2015, 162 invasive GBS isolates were collected from 86 health care centers in 32 Argentinean cities. All isolates were screened for FQ nonsusceptibility using a five-disc scheme: Levofloxacin (LVX), ciprofloxacin, norfloxacin (NOR), ofloxacin, and pefloxacin (PF). LVX minimal inhibitory concentration (MIC) was determined by the agar dilution method. Sequencing of internal regions of gyrA and parC genes was performed. Capsular typing and genetic characterization of nonsusceptible isolates were assessed by latex agglutination, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing. Results: Twenty-four of one hundred sixty-two GBS isolates exhibited no inhibition zones to all tested FQs with an MIC range of 16-32 mg/L for LVX, and one isolate with MIC = 1 mg/L showed no inhibition zones around NOR and PF discs. In all resistant isolates, point mutations were detected in both genes. Serotype Ib was prevalent (88%). One PFGE type accounted for 84% of the FQ-resistant isolates and belonged to serotype Ib, sequence type 10. Conclusions: The prevalence of FQ resistance was 14.8% likely to be associated with dissemination of an ST10/serotype Ib clone. The unexpected high rate of resistance emphasizes the relevance for continuous surveillance of GBS epidemiology and antibiotic susceptibility.
publishDate 2019
dc.date.none.fl_str_mv 2019-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/130190
Arias, Bárbara Soledad; Kovacec, Verónica Ivana; Vigliarolo, Laura; Suárez, Mariana; Tersigni, Carina Laura; et al.; Fluoroquinolone-resistant streptococcus agalactiae invasive isolates recovered in Argentina; Mary Ann Liebert; Microbial Drug Resistance: Mechanisms Epidemiology and Disease; 25; 5; 6-2019; 739-743
1076-6294
CONICET Digital
CONICET
url http://hdl.handle.net/11336/130190
identifier_str_mv Arias, Bárbara Soledad; Kovacec, Verónica Ivana; Vigliarolo, Laura; Suárez, Mariana; Tersigni, Carina Laura; et al.; Fluoroquinolone-resistant streptococcus agalactiae invasive isolates recovered in Argentina; Mary Ann Liebert; Microbial Drug Resistance: Mechanisms Epidemiology and Disease; 25; 5; 6-2019; 739-743
1076-6294
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1089/mdr.2018.0246
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert
publisher.none.fl_str_mv Mary Ann Liebert
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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