SnRK1 subcellular localization is linked to TOR signaling, chloroplast function, and salt stress tolerance in mature Arabidopsis leaves

Autores
Lando, Ana Paula; Maritano, Paula Catalina; Barbieri, Giuliano; Danilo, Daloso; Martínez Noël, Giselle María Astrid; Rodriguez, Marianela Soledad
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Soil salinity can impair carbon fixation and ultimately decrease crop yield. To counteract this detrimental effect and maintain cellular homeostasis and productivity under stress, plants rely on metabolic and growth adaptations. Sucrose non-fermenting Related Kinase 1 (SnRK1) and Target of Rapamycin (TOR) kinases coordinate cellular metabolism and stress signaling. Despite substantial progress, major gaps persist regarding how SnRK1 subcellular localization affects TOR activity, chloroplast function, and overall metabolic balance under salinity in source leaves. We analyzed Arabidopsis mature leaves of plants expressing SnRK1α1 targeted to the nucleus (NLS-α1) or membrane/myristoylation (βMYR-α1) subjected to prolonged salinity (150 mM NaCl, 14 days). Under control conditions, SnRK1 activity was low during the light period in wild-type (WT) leaves, however under salinity, both total and nuclear SnRK1 activity rose markedly at midday. Remarkably, the NLS-α1 plants exhibited increased tolerance, showing 10% less shoot biomass reduction than WT plants under salt stress. This may be related to the fact that mature leaves of NLS-α1 genotype showed intact chloroplast ultrastructure, higher photosynthetic performance, and elevated levels of threonine and stress-related metabolites under NaCl long-term exposure. Conversely, βMYR-α1 plants exhibited misregulated TOR activity, disrupted thylakoid structure with reduced photosynthetic efficiency and photodamage at PSII level, and the accumulation of photorespiratory intermediates. Overall, SnRK1 localization emerges as a spatial factor associated with organelle performance, metabolic reprogramming, and TOR signaling patterns during salt stress. Modulating SnRK1 intracellular distribution may, therefore, be a useful strategy for breeding or biotechnological approaches toward plant salinity tolerance.
Fil: Lando, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Maritano, Paula Catalina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Estudios Agropecuarios; Argentina
Fil: Barbieri, Giuliano. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Estudios Agropecuarios; Argentina
Fil: Danilo, Daloso. Universidade Federal do Ceara; Brasil
Fil: Martínez Noël, Giselle María Astrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Rodriguez, Marianela Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Estudios Agropecuarios; Argentina
Materia
SnRK1 kinase
salt-stress
TOR kinase
chloroplast
metabolism
sugar signal
chlorophyll fluorescence
Arabidopsis
Nivel de accesibilidad
acceso embargado
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/287889
Acceder
id CONICETDig_75f64db60da4fe60d0547c5a54bb23b7
oai_identifier_str oai:ri.conicet.gov.ar:11336/287889
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling SnRK1 subcellular localization is linked to TOR signaling, chloroplast function, and salt stress tolerance in mature Arabidopsis leavesLando, Ana PaulaMaritano, Paula CatalinaBarbieri, GiulianoDanilo, DalosoMartínez Noël, Giselle María AstridRodriguez, Marianela SoledadSnRK1 kinasesalt-stressTOR kinasechloroplastmetabolismsugar signalchlorophyll fluorescenceArabidopsishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Soil salinity can impair carbon fixation and ultimately decrease crop yield. To counteract this detrimental effect and maintain cellular homeostasis and productivity under stress, plants rely on metabolic and growth adaptations. Sucrose non-fermenting Related Kinase 1 (SnRK1) and Target of Rapamycin (TOR) kinases coordinate cellular metabolism and stress signaling. Despite substantial progress, major gaps persist regarding how SnRK1 subcellular localization affects TOR activity, chloroplast function, and overall metabolic balance under salinity in source leaves. We analyzed Arabidopsis mature leaves of plants expressing SnRK1α1 targeted to the nucleus (NLS-α1) or membrane/myristoylation (βMYR-α1) subjected to prolonged salinity (150 mM NaCl, 14 days). Under control conditions, SnRK1 activity was low during the light period in wild-type (WT) leaves, however under salinity, both total and nuclear SnRK1 activity rose markedly at midday. Remarkably, the NLS-α1 plants exhibited increased tolerance, showing 10% less shoot biomass reduction than WT plants under salt stress. This may be related to the fact that mature leaves of NLS-α1 genotype showed intact chloroplast ultrastructure, higher photosynthetic performance, and elevated levels of threonine and stress-related metabolites under NaCl long-term exposure. Conversely, βMYR-α1 plants exhibited misregulated TOR activity, disrupted thylakoid structure with reduced photosynthetic efficiency and photodamage at PSII level, and the accumulation of photorespiratory intermediates. Overall, SnRK1 localization emerges as a spatial factor associated with organelle performance, metabolic reprogramming, and TOR signaling patterns during salt stress. Modulating SnRK1 intracellular distribution may, therefore, be a useful strategy for breeding or biotechnological approaches toward plant salinity tolerance.Fil: Lando, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Maritano, Paula Catalina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Estudios Agropecuarios; ArgentinaFil: Barbieri, Giuliano. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Estudios Agropecuarios; ArgentinaFil: Danilo, Daloso. Universidade Federal do Ceara; BrasilFil: Martínez Noël, Giselle María Astrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Rodriguez, Marianela Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Estudios Agropecuarios; ArgentinaElsevier Ireland2026-03info:eu-repo/date/embargoEnd/2026-09-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/287889Lando, Ana Paula; Maritano, Paula Catalina; Barbieri, Giuliano; Danilo, Daloso; Martínez Noël, Giselle María Astrid; et al.; SnRK1 subcellular localization is linked to TOR signaling, chloroplast function, and salt stress tolerance in mature Arabidopsis leaves; Elsevier Ireland; Plant Science; 3-2026; 1-410168-9452CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0168945226001573info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2026.113129info:eu-repo/semantics/embargoedAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-06-17T09:37:26Zoai:ri.conicet.gov.ar:11336/287889instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-06-17 09:37:26.789CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv SnRK1 subcellular localization is linked to TOR signaling, chloroplast function, and salt stress tolerance in mature Arabidopsis leaves
title SnRK1 subcellular localization is linked to TOR signaling, chloroplast function, and salt stress tolerance in mature Arabidopsis leaves
spellingShingle SnRK1 subcellular localization is linked to TOR signaling, chloroplast function, and salt stress tolerance in mature Arabidopsis leaves
Lando, Ana Paula
SnRK1 kinase
salt-stress
TOR kinase
chloroplast
metabolism
sugar signal
chlorophyll fluorescence
Arabidopsis
title_short SnRK1 subcellular localization is linked to TOR signaling, chloroplast function, and salt stress tolerance in mature Arabidopsis leaves
title_full SnRK1 subcellular localization is linked to TOR signaling, chloroplast function, and salt stress tolerance in mature Arabidopsis leaves
title_fullStr SnRK1 subcellular localization is linked to TOR signaling, chloroplast function, and salt stress tolerance in mature Arabidopsis leaves
title_full_unstemmed SnRK1 subcellular localization is linked to TOR signaling, chloroplast function, and salt stress tolerance in mature Arabidopsis leaves
title_sort SnRK1 subcellular localization is linked to TOR signaling, chloroplast function, and salt stress tolerance in mature Arabidopsis leaves
dc.creator.none.fl_str_mv Lando, Ana Paula
Maritano, Paula Catalina
Barbieri, Giuliano
Danilo, Daloso
Martínez Noël, Giselle María Astrid
Rodriguez, Marianela Soledad
author Lando, Ana Paula
author_facet Lando, Ana Paula
Maritano, Paula Catalina
Barbieri, Giuliano
Danilo, Daloso
Martínez Noël, Giselle María Astrid
Rodriguez, Marianela Soledad
author_role author
author2 Maritano, Paula Catalina
Barbieri, Giuliano
Danilo, Daloso
Martínez Noël, Giselle María Astrid
Rodriguez, Marianela Soledad
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv SnRK1 kinase
salt-stress
TOR kinase
chloroplast
metabolism
sugar signal
chlorophyll fluorescence
Arabidopsis
topic SnRK1 kinase
salt-stress
TOR kinase
chloroplast
metabolism
sugar signal
chlorophyll fluorescence
Arabidopsis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Soil salinity can impair carbon fixation and ultimately decrease crop yield. To counteract this detrimental effect and maintain cellular homeostasis and productivity under stress, plants rely on metabolic and growth adaptations. Sucrose non-fermenting Related Kinase 1 (SnRK1) and Target of Rapamycin (TOR) kinases coordinate cellular metabolism and stress signaling. Despite substantial progress, major gaps persist regarding how SnRK1 subcellular localization affects TOR activity, chloroplast function, and overall metabolic balance under salinity in source leaves. We analyzed Arabidopsis mature leaves of plants expressing SnRK1α1 targeted to the nucleus (NLS-α1) or membrane/myristoylation (βMYR-α1) subjected to prolonged salinity (150 mM NaCl, 14 days). Under control conditions, SnRK1 activity was low during the light period in wild-type (WT) leaves, however under salinity, both total and nuclear SnRK1 activity rose markedly at midday. Remarkably, the NLS-α1 plants exhibited increased tolerance, showing 10% less shoot biomass reduction than WT plants under salt stress. This may be related to the fact that mature leaves of NLS-α1 genotype showed intact chloroplast ultrastructure, higher photosynthetic performance, and elevated levels of threonine and stress-related metabolites under NaCl long-term exposure. Conversely, βMYR-α1 plants exhibited misregulated TOR activity, disrupted thylakoid structure with reduced photosynthetic efficiency and photodamage at PSII level, and the accumulation of photorespiratory intermediates. Overall, SnRK1 localization emerges as a spatial factor associated with organelle performance, metabolic reprogramming, and TOR signaling patterns during salt stress. Modulating SnRK1 intracellular distribution may, therefore, be a useful strategy for breeding or biotechnological approaches toward plant salinity tolerance.
Fil: Lando, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Maritano, Paula Catalina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Estudios Agropecuarios; Argentina
Fil: Barbieri, Giuliano. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Estudios Agropecuarios; Argentina
Fil: Danilo, Daloso. Universidade Federal do Ceara; Brasil
Fil: Martínez Noël, Giselle María Astrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Rodriguez, Marianela Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Estudios Agropecuarios; Argentina
description Soil salinity can impair carbon fixation and ultimately decrease crop yield. To counteract this detrimental effect and maintain cellular homeostasis and productivity under stress, plants rely on metabolic and growth adaptations. Sucrose non-fermenting Related Kinase 1 (SnRK1) and Target of Rapamycin (TOR) kinases coordinate cellular metabolism and stress signaling. Despite substantial progress, major gaps persist regarding how SnRK1 subcellular localization affects TOR activity, chloroplast function, and overall metabolic balance under salinity in source leaves. We analyzed Arabidopsis mature leaves of plants expressing SnRK1α1 targeted to the nucleus (NLS-α1) or membrane/myristoylation (βMYR-α1) subjected to prolonged salinity (150 mM NaCl, 14 days). Under control conditions, SnRK1 activity was low during the light period in wild-type (WT) leaves, however under salinity, both total and nuclear SnRK1 activity rose markedly at midday. Remarkably, the NLS-α1 plants exhibited increased tolerance, showing 10% less shoot biomass reduction than WT plants under salt stress. This may be related to the fact that mature leaves of NLS-α1 genotype showed intact chloroplast ultrastructure, higher photosynthetic performance, and elevated levels of threonine and stress-related metabolites under NaCl long-term exposure. Conversely, βMYR-α1 plants exhibited misregulated TOR activity, disrupted thylakoid structure with reduced photosynthetic efficiency and photodamage at PSII level, and the accumulation of photorespiratory intermediates. Overall, SnRK1 localization emerges as a spatial factor associated with organelle performance, metabolic reprogramming, and TOR signaling patterns during salt stress. Modulating SnRK1 intracellular distribution may, therefore, be a useful strategy for breeding or biotechnological approaches toward plant salinity tolerance.
publishDate 2026
dc.date.none.fl_str_mv 2026-03
info:eu-repo/date/embargoEnd/2026-09-22
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/287889
Lando, Ana Paula; Maritano, Paula Catalina; Barbieri, Giuliano; Danilo, Daloso; Martínez Noël, Giselle María Astrid; et al.; SnRK1 subcellular localization is linked to TOR signaling, chloroplast function, and salt stress tolerance in mature Arabidopsis leaves; Elsevier Ireland; Plant Science; 3-2026; 1-41
0168-9452
CONICET Digital
CONICET
url http://hdl.handle.net/11336/287889
identifier_str_mv Lando, Ana Paula; Maritano, Paula Catalina; Barbieri, Giuliano; Danilo, Daloso; Martínez Noël, Giselle María Astrid; et al.; SnRK1 subcellular localization is linked to TOR signaling, chloroplast function, and salt stress tolerance in mature Arabidopsis leaves; Elsevier Ireland; Plant Science; 3-2026; 1-41
0168-9452
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0168945226001573
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2026.113129
dc.rights.none.fl_str_mv info:eu-repo/semantics/embargoedAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv embargoedAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.040872

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