Dual and dynamic intracellular localization of Arabidopsis thaliana SnRK1.1

Autores
Blanco, Nicolás Ernesto; Liebsch, Daniela Elisa; Guinea Díaz, Manuel; Strand, Asa; Whelan, James
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sucrose non-fermenting 1 (SNF1)-related protein kinase 1.1 (SnRK1.1; also known as KIN10 or SnRK1α) has been identified as the catalytic subunit of the complex SnRK1, the Arabidopsis thaliana homologue of a central integrator of energy and stress signalling in eukaryotes dubbed AMPK/Snf1/SnRK1. A nuclear localization of SnRK1.1 has been previously described and is in line with its function as an integrator of energy and stress signals. Here, using two biological models (Nicotiana benthamiana and Arabidopsis thaliana), native regulatory sequences, different microscopy techniques, and manipulations of cellular energy status, it was found that SnRK1.1 is localized dynamically between the nucleus and endoplasmic reticulum (ER). This distribution was confirmed at a spatial and temporal level by co-localization studies with two different fluorescent ER markers, one of them being the SnRK1.1 phosphorylation target HMGR. The ER and nuclear localization displayed a dynamic behaviour in response to perturbations of the plastidic electron transport chain. These results suggest that an ER-associated SnRK1.1 fraction might be sensing the cellular energy status, being a point of crosstalk with other ER stress regulatory pathways.
Fil: Blanco, Nicolás Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad de Umea; Suecia
Fil: Liebsch, Daniela Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad de Umea; Suecia
Fil: Guinea Díaz, Manuel. University of Turku. Department of Biochemistry. Molecular Plant Biology; Finlandia
Fil: Strand, Asa. Universidad de Umea; Suecia
Fil: Whelan, James. La Trobe University; Australia
Materia
ARABIDOPSIS
CHLOROPLAST
DUAL LOCALIZATION
ENDOPLASMIC RETICULUM (ER)
ENERGY STATUS
ER LOCALIZATION
NICOTIANA BENTHAMIANA
NUCLEAR LOCALIZATION
RETROGRADE SIGNALLING
SNRK1.1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/149434
Acceder
id CONICETDig_c2c7feea5aa45b5d217bc427a93b7273
oai_identifier_str oai:ri.conicet.gov.ar:11336/149434
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dual and dynamic intracellular localization of Arabidopsis thaliana SnRK1.1Blanco, Nicolás ErnestoLiebsch, Daniela ElisaGuinea Díaz, ManuelStrand, AsaWhelan, JamesARABIDOPSISCHLOROPLASTDUAL LOCALIZATIONENDOPLASMIC RETICULUM (ER)ENERGY STATUSER LOCALIZATIONNICOTIANA BENTHAMIANANUCLEAR LOCALIZATIONRETROGRADE SIGNALLINGSNRK1.1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Sucrose non-fermenting 1 (SNF1)-related protein kinase 1.1 (SnRK1.1; also known as KIN10 or SnRK1α) has been identified as the catalytic subunit of the complex SnRK1, the Arabidopsis thaliana homologue of a central integrator of energy and stress signalling in eukaryotes dubbed AMPK/Snf1/SnRK1. A nuclear localization of SnRK1.1 has been previously described and is in line with its function as an integrator of energy and stress signals. Here, using two biological models (Nicotiana benthamiana and Arabidopsis thaliana), native regulatory sequences, different microscopy techniques, and manipulations of cellular energy status, it was found that SnRK1.1 is localized dynamically between the nucleus and endoplasmic reticulum (ER). This distribution was confirmed at a spatial and temporal level by co-localization studies with two different fluorescent ER markers, one of them being the SnRK1.1 phosphorylation target HMGR. The ER and nuclear localization displayed a dynamic behaviour in response to perturbations of the plastidic electron transport chain. These results suggest that an ER-associated SnRK1.1 fraction might be sensing the cellular energy status, being a point of crosstalk with other ER stress regulatory pathways.Fil: Blanco, Nicolás Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad de Umea; SueciaFil: Liebsch, Daniela Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad de Umea; SueciaFil: Guinea Díaz, Manuel. University of Turku. Department of Biochemistry. Molecular Plant Biology; FinlandiaFil: Strand, Asa. Universidad de Umea; SueciaFil: Whelan, James. La Trobe University; AustraliaOxford University Press2019-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/149434Blanco, Nicolás Ernesto; Liebsch, Daniela Elisa; Guinea Díaz, Manuel; Strand, Asa; Whelan, James; Dual and dynamic intracellular localization of Arabidopsis thaliana SnRK1.1; Oxford University Press; Journal of Experimental Botany; 70; 8; 4-2019; 2325-23380022-0957CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/article/70/8/2325/5308885info:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/erz023info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:08Zoai:ri.conicet.gov.ar:11336/149434instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:08.825CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dual and dynamic intracellular localization of Arabidopsis thaliana SnRK1.1
title Dual and dynamic intracellular localization of Arabidopsis thaliana SnRK1.1
spellingShingle Dual and dynamic intracellular localization of Arabidopsis thaliana SnRK1.1
Blanco, Nicolás Ernesto
ARABIDOPSIS
CHLOROPLAST
DUAL LOCALIZATION
ENDOPLASMIC RETICULUM (ER)
ENERGY STATUS
ER LOCALIZATION
NICOTIANA BENTHAMIANA
NUCLEAR LOCALIZATION
RETROGRADE SIGNALLING
SNRK1.1
title_short Dual and dynamic intracellular localization of Arabidopsis thaliana SnRK1.1
title_full Dual and dynamic intracellular localization of Arabidopsis thaliana SnRK1.1
title_fullStr Dual and dynamic intracellular localization of Arabidopsis thaliana SnRK1.1
title_full_unstemmed Dual and dynamic intracellular localization of Arabidopsis thaliana SnRK1.1
title_sort Dual and dynamic intracellular localization of Arabidopsis thaliana SnRK1.1
dc.creator.none.fl_str_mv Blanco, Nicolás Ernesto
Liebsch, Daniela Elisa
Guinea Díaz, Manuel
Strand, Asa
Whelan, James
author Blanco, Nicolás Ernesto
author_facet Blanco, Nicolás Ernesto
Liebsch, Daniela Elisa
Guinea Díaz, Manuel
Strand, Asa
Whelan, James
author_role author
author2 Liebsch, Daniela Elisa
Guinea Díaz, Manuel
Strand, Asa
Whelan, James
author2_role author
author
author
author
dc.subject.none.fl_str_mv ARABIDOPSIS
CHLOROPLAST
DUAL LOCALIZATION
ENDOPLASMIC RETICULUM (ER)
ENERGY STATUS
ER LOCALIZATION
NICOTIANA BENTHAMIANA
NUCLEAR LOCALIZATION
RETROGRADE SIGNALLING
SNRK1.1
topic ARABIDOPSIS
CHLOROPLAST
DUAL LOCALIZATION
ENDOPLASMIC RETICULUM (ER)
ENERGY STATUS
ER LOCALIZATION
NICOTIANA BENTHAMIANA
NUCLEAR LOCALIZATION
RETROGRADE SIGNALLING
SNRK1.1
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Sucrose non-fermenting 1 (SNF1)-related protein kinase 1.1 (SnRK1.1; also known as KIN10 or SnRK1α) has been identified as the catalytic subunit of the complex SnRK1, the Arabidopsis thaliana homologue of a central integrator of energy and stress signalling in eukaryotes dubbed AMPK/Snf1/SnRK1. A nuclear localization of SnRK1.1 has been previously described and is in line with its function as an integrator of energy and stress signals. Here, using two biological models (Nicotiana benthamiana and Arabidopsis thaliana), native regulatory sequences, different microscopy techniques, and manipulations of cellular energy status, it was found that SnRK1.1 is localized dynamically between the nucleus and endoplasmic reticulum (ER). This distribution was confirmed at a spatial and temporal level by co-localization studies with two different fluorescent ER markers, one of them being the SnRK1.1 phosphorylation target HMGR. The ER and nuclear localization displayed a dynamic behaviour in response to perturbations of the plastidic electron transport chain. These results suggest that an ER-associated SnRK1.1 fraction might be sensing the cellular energy status, being a point of crosstalk with other ER stress regulatory pathways.
Fil: Blanco, Nicolás Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad de Umea; Suecia
Fil: Liebsch, Daniela Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad de Umea; Suecia
Fil: Guinea Díaz, Manuel. University of Turku. Department of Biochemistry. Molecular Plant Biology; Finlandia
Fil: Strand, Asa. Universidad de Umea; Suecia
Fil: Whelan, James. La Trobe University; Australia
description Sucrose non-fermenting 1 (SNF1)-related protein kinase 1.1 (SnRK1.1; also known as KIN10 or SnRK1α) has been identified as the catalytic subunit of the complex SnRK1, the Arabidopsis thaliana homologue of a central integrator of energy and stress signalling in eukaryotes dubbed AMPK/Snf1/SnRK1. A nuclear localization of SnRK1.1 has been previously described and is in line with its function as an integrator of energy and stress signals. Here, using two biological models (Nicotiana benthamiana and Arabidopsis thaliana), native regulatory sequences, different microscopy techniques, and manipulations of cellular energy status, it was found that SnRK1.1 is localized dynamically between the nucleus and endoplasmic reticulum (ER). This distribution was confirmed at a spatial and temporal level by co-localization studies with two different fluorescent ER markers, one of them being the SnRK1.1 phosphorylation target HMGR. The ER and nuclear localization displayed a dynamic behaviour in response to perturbations of the plastidic electron transport chain. These results suggest that an ER-associated SnRK1.1 fraction might be sensing the cellular energy status, being a point of crosstalk with other ER stress regulatory pathways.
publishDate 2019
dc.date.none.fl_str_mv 2019-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/149434
Blanco, Nicolás Ernesto; Liebsch, Daniela Elisa; Guinea Díaz, Manuel; Strand, Asa; Whelan, James; Dual and dynamic intracellular localization of Arabidopsis thaliana SnRK1.1; Oxford University Press; Journal of Experimental Botany; 70; 8; 4-2019; 2325-2338
0022-0957
CONICET Digital
CONICET
url http://hdl.handle.net/11336/149434
identifier_str_mv Blanco, Nicolás Ernesto; Liebsch, Daniela Elisa; Guinea Díaz, Manuel; Strand, Asa; Whelan, James; Dual and dynamic intracellular localization of Arabidopsis thaliana SnRK1.1; Oxford University Press; Journal of Experimental Botany; 70; 8; 4-2019; 2325-2338
0022-0957
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/article/70/8/2325/5308885
info:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/erz023
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613906746572800
score 13.070432

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