Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura)

Autores
Czuchlej, Silvia Cristina; Volonteri, María Clara; Regueira, Eleonora; Ceballos, Nora Raquel
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In rat Leydig cells, glucocorticoids (GCs) inhibit testosterone production through the interaction with the glucocorticoid receptor (GR). However, the sensitivity of those cells to GCs is regulated by the enzyme 11β‐hydroxysteroid dehydrogenase Type 1 (11β‐HSD1). In the testes of the toad Rhinella arenarum, the presence of an 11β‐HSD similar to type 2 and a cytosolic GR has also been described. However, there is a lack of information regarding the effects of GCs on amphibian testicular steroidogenesis. In this study, the effects of corticosterone on androgen production, and the activity of two steroidogenic enzymes in toad testes were reported. Corticosterone inhibits androgen production via the GR because the GR antagonist RU486 prevents corticosterone‐induced inhibition of testosterone. Corticosterone also reduced the activity of the cytochrome P450 17‐hydroxylase, C17,20‐lyase (Cyp450c17) without affecting the 3β‐hydroxysteroid dehydrogenase/isomerase activity. This effect on Cyp450c17 was likewise inhibited by RU486. On the other hand, corticosterone had no effect on the amount of steroidogenic acute regulator protein. These results suggest that GCs inhibit steroidogenesis in toad testes by reducing of Cyp450c17 activity via a GR‐mediated mechanism.
Fil: Czuchlej, Silvia Cristina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Volonteri, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Diversidad y Evolución Austral; Argentina
Fil: Regueira, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Ceballos, Nora Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Materia
AMPHIBIANS
ANDROGENS
GLUCOCORTICOIDS (GC)
STEROIDOGENESIS
TESTES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/92310
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/92310
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura)Czuchlej, Silvia CristinaVolonteri, María ClaraRegueira, EleonoraCeballos, Nora RaquelAMPHIBIANSANDROGENSGLUCOCORTICOIDS (GC)STEROIDOGENESISTESTEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In rat Leydig cells, glucocorticoids (GCs) inhibit testosterone production through the interaction with the glucocorticoid receptor (GR). However, the sensitivity of those cells to GCs is regulated by the enzyme 11β‐hydroxysteroid dehydrogenase Type 1 (11β‐HSD1). In the testes of the toad Rhinella arenarum, the presence of an 11β‐HSD similar to type 2 and a cytosolic GR has also been described. However, there is a lack of information regarding the effects of GCs on amphibian testicular steroidogenesis. In this study, the effects of corticosterone on androgen production, and the activity of two steroidogenic enzymes in toad testes were reported. Corticosterone inhibits androgen production via the GR because the GR antagonist RU486 prevents corticosterone‐induced inhibition of testosterone. Corticosterone also reduced the activity of the cytochrome P450 17‐hydroxylase, C17,20‐lyase (Cyp450c17) without affecting the 3β‐hydroxysteroid dehydrogenase/isomerase activity. This effect on Cyp450c17 was likewise inhibited by RU486. On the other hand, corticosterone had no effect on the amount of steroidogenic acute regulator protein. These results suggest that GCs inhibit steroidogenesis in toad testes by reducing of Cyp450c17 activity via a GR‐mediated mechanism.Fil: Czuchlej, Silvia Cristina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Volonteri, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Diversidad y Evolución Austral; ArgentinaFil: Regueira, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Ceballos, Nora Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaJohn Wiley & Sons Inc2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/92310Czuchlej, Silvia Cristina; Volonteri, María Clara; Regueira, Eleonora; Ceballos, Nora Raquel; Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura); John Wiley & Sons Inc; Journal of Experimental Zoology Part A: Ecological and Integrative Physiology; 331; 1; 12-2018; 17-262471-56382471-5646CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/jez.2232info:eu-repo/semantics/altIdentifier/doi/10.1002/jez.2232info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:46Zoai:ri.conicet.gov.ar:11336/92310instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:46.743CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura)
title Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura)
spellingShingle Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura)
Czuchlej, Silvia Cristina
AMPHIBIANS
ANDROGENS
GLUCOCORTICOIDS (GC)
STEROIDOGENESIS
TESTES
title_short Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura)
title_full Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura)
title_fullStr Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura)
title_full_unstemmed Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura)
title_sort Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura)
dc.creator.none.fl_str_mv Czuchlej, Silvia Cristina
Volonteri, María Clara
Regueira, Eleonora
Ceballos, Nora Raquel
author Czuchlej, Silvia Cristina
author_facet Czuchlej, Silvia Cristina
Volonteri, María Clara
Regueira, Eleonora
Ceballos, Nora Raquel
author_role author
author2 Volonteri, María Clara
Regueira, Eleonora
Ceballos, Nora Raquel
author2_role author
author
author
dc.subject.none.fl_str_mv AMPHIBIANS
ANDROGENS
GLUCOCORTICOIDS (GC)
STEROIDOGENESIS
TESTES
topic AMPHIBIANS
ANDROGENS
GLUCOCORTICOIDS (GC)
STEROIDOGENESIS
TESTES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In rat Leydig cells, glucocorticoids (GCs) inhibit testosterone production through the interaction with the glucocorticoid receptor (GR). However, the sensitivity of those cells to GCs is regulated by the enzyme 11β‐hydroxysteroid dehydrogenase Type 1 (11β‐HSD1). In the testes of the toad Rhinella arenarum, the presence of an 11β‐HSD similar to type 2 and a cytosolic GR has also been described. However, there is a lack of information regarding the effects of GCs on amphibian testicular steroidogenesis. In this study, the effects of corticosterone on androgen production, and the activity of two steroidogenic enzymes in toad testes were reported. Corticosterone inhibits androgen production via the GR because the GR antagonist RU486 prevents corticosterone‐induced inhibition of testosterone. Corticosterone also reduced the activity of the cytochrome P450 17‐hydroxylase, C17,20‐lyase (Cyp450c17) without affecting the 3β‐hydroxysteroid dehydrogenase/isomerase activity. This effect on Cyp450c17 was likewise inhibited by RU486. On the other hand, corticosterone had no effect on the amount of steroidogenic acute regulator protein. These results suggest that GCs inhibit steroidogenesis in toad testes by reducing of Cyp450c17 activity via a GR‐mediated mechanism.
Fil: Czuchlej, Silvia Cristina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Volonteri, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Diversidad y Evolución Austral; Argentina
Fil: Regueira, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Ceballos, Nora Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
description In rat Leydig cells, glucocorticoids (GCs) inhibit testosterone production through the interaction with the glucocorticoid receptor (GR). However, the sensitivity of those cells to GCs is regulated by the enzyme 11β‐hydroxysteroid dehydrogenase Type 1 (11β‐HSD1). In the testes of the toad Rhinella arenarum, the presence of an 11β‐HSD similar to type 2 and a cytosolic GR has also been described. However, there is a lack of information regarding the effects of GCs on amphibian testicular steroidogenesis. In this study, the effects of corticosterone on androgen production, and the activity of two steroidogenic enzymes in toad testes were reported. Corticosterone inhibits androgen production via the GR because the GR antagonist RU486 prevents corticosterone‐induced inhibition of testosterone. Corticosterone also reduced the activity of the cytochrome P450 17‐hydroxylase, C17,20‐lyase (Cyp450c17) without affecting the 3β‐hydroxysteroid dehydrogenase/isomerase activity. This effect on Cyp450c17 was likewise inhibited by RU486. On the other hand, corticosterone had no effect on the amount of steroidogenic acute regulator protein. These results suggest that GCs inhibit steroidogenesis in toad testes by reducing of Cyp450c17 activity via a GR‐mediated mechanism.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/92310
Czuchlej, Silvia Cristina; Volonteri, María Clara; Regueira, Eleonora; Ceballos, Nora Raquel; Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura); John Wiley & Sons Inc; Journal of Experimental Zoology Part A: Ecological and Integrative Physiology; 331; 1; 12-2018; 17-26
2471-5638
2471-5646
CONICET Digital
CONICET
url http://hdl.handle.net/11336/92310
identifier_str_mv Czuchlej, Silvia Cristina; Volonteri, María Clara; Regueira, Eleonora; Ceballos, Nora Raquel; Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura); John Wiley & Sons Inc; Journal of Experimental Zoology Part A: Ecological and Integrative Physiology; 331; 1; 12-2018; 17-26
2471-5638
2471-5646
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/jez.2232
info:eu-repo/semantics/altIdentifier/doi/10.1002/jez.2232
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Inc
publisher.none.fl_str_mv John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268880564649984
score 13.13397

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