Seasonal Changes and Regulation of the Glucocorticoid Receptor in the Testis of the Toad Rhinella arenarum

Autores
Regueira, Eleonora; Sassone, Alina Grisel; Scaia, María Florencia; Volonteri, María Clara; Ceballos, Nora Raquel
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Several studies indicate that wild free‐living vertebrates seasonally regulate plasma glucocorticoids. However, not only glucocorticoids but also the amount of receptors is important in determining biological responses. In this context, seasonal regulation of glucocorticoid receptor (GR) is crucial to modulate the response to glucocorticoids. Rhinella arenarum is an anuran exhibiting seasonal variations in plasma glucocorticoids and also in the number of binding sites (Bmax) of the testicular cytosolic GR. In this work, we evaluated if the annual pattern of GR protein in the testis varies seasonally and, by an in vitro approach, the role of glucocorticoids, androgens, and melatonin in the regulation of the GR Bmax and protein level. For this purpose, testes were treated with two physiological concentrations of melatonin (40 and 200 pg/ml), with or without luzindole (melatonin‐receptor antagonist); with testosterone, cyanoketone (inhibitor of steroidogenesis) or casodex (androgen‐receptor antagonist); or with dexamethasone or RU486 (GR antagonist). After treatments, Bmax and protein level were determined by the binding of [3 H]dexamethasone and Western blot, respectively. Results showed that GR protein decreases in the winter. The in vitro treatment with melatonin produced a biphasic effect on the Bmax with the lowest concentration decreasing this parameter by a receptor‐mediated mechanism. However, melatonin had no effect on the GR protein level. Conversely, a high concentration of dexamethasone up‐regulated the GR protein and androgens neither changed the Bmax nor the protein level. These findings suggest that seasonal changes in plasma melatonin and glucocorticoids modulate the effect of glucocorticoids in the testis of R. arenarum.
Fil: Regueira, Eleonora. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sassone, Alina Grisel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Scaia, María Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Volonteri, María Clara. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ceballos, Nora Raquel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Toad
Glucocorticoids
Glucocorticoid Receptor
Androgens
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/21301
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/21301
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Seasonal Changes and Regulation of the Glucocorticoid Receptor in the Testis of the Toad Rhinella arenarumRegueira, EleonoraSassone, Alina GriselScaia, María FlorenciaVolonteri, María ClaraCeballos, Nora RaquelToadGlucocorticoidsGlucocorticoid ReceptorAndrogenshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Several studies indicate that wild free‐living vertebrates seasonally regulate plasma glucocorticoids. However, not only glucocorticoids but also the amount of receptors is important in determining biological responses. In this context, seasonal regulation of glucocorticoid receptor (GR) is crucial to modulate the response to glucocorticoids. Rhinella arenarum is an anuran exhibiting seasonal variations in plasma glucocorticoids and also in the number of binding sites (Bmax) of the testicular cytosolic GR. In this work, we evaluated if the annual pattern of GR protein in the testis varies seasonally and, by an in vitro approach, the role of glucocorticoids, androgens, and melatonin in the regulation of the GR Bmax and protein level. For this purpose, testes were treated with two physiological concentrations of melatonin (40 and 200 pg/ml), with or without luzindole (melatonin‐receptor antagonist); with testosterone, cyanoketone (inhibitor of steroidogenesis) or casodex (androgen‐receptor antagonist); or with dexamethasone or RU486 (GR antagonist). After treatments, Bmax and protein level were determined by the binding of [3 H]dexamethasone and Western blot, respectively. Results showed that GR protein decreases in the winter. The in vitro treatment with melatonin produced a biphasic effect on the Bmax with the lowest concentration decreasing this parameter by a receptor‐mediated mechanism. However, melatonin had no effect on the GR protein level. Conversely, a high concentration of dexamethasone up‐regulated the GR protein and androgens neither changed the Bmax nor the protein level. These findings suggest that seasonal changes in plasma melatonin and glucocorticoids modulate the effect of glucocorticoids in the testis of R. arenarum.Fil: Regueira, Eleonora. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sassone, Alina Grisel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Scaia, María Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Volonteri, María Clara. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ceballos, Nora Raquel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley2012-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/21301Regueira, Eleonora; Sassone, Alina Grisel; Scaia, María Florencia; Volonteri, María Clara; Ceballos, Nora Raquel; Seasonal Changes and Regulation of the Glucocorticoid Receptor in the Testis of the Toad Rhinella arenarum; Wiley; Journal of Experimental Zoology Part A: Ecological Genetics and Physiology; 319; 1; 11-2012; 39-521932-5223CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jez.1772info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jez.1772/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:13:55Zoai:ri.conicet.gov.ar:11336/21301instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:13:55.801CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Seasonal Changes and Regulation of the Glucocorticoid Receptor in the Testis of the Toad Rhinella arenarum
title Seasonal Changes and Regulation of the Glucocorticoid Receptor in the Testis of the Toad Rhinella arenarum
spellingShingle Seasonal Changes and Regulation of the Glucocorticoid Receptor in the Testis of the Toad Rhinella arenarum
Regueira, Eleonora
Toad
Glucocorticoids
Glucocorticoid Receptor
Androgens
title_short Seasonal Changes and Regulation of the Glucocorticoid Receptor in the Testis of the Toad Rhinella arenarum
title_full Seasonal Changes and Regulation of the Glucocorticoid Receptor in the Testis of the Toad Rhinella arenarum
title_fullStr Seasonal Changes and Regulation of the Glucocorticoid Receptor in the Testis of the Toad Rhinella arenarum
title_full_unstemmed Seasonal Changes and Regulation of the Glucocorticoid Receptor in the Testis of the Toad Rhinella arenarum
title_sort Seasonal Changes and Regulation of the Glucocorticoid Receptor in the Testis of the Toad Rhinella arenarum
dc.creator.none.fl_str_mv Regueira, Eleonora
Sassone, Alina Grisel
Scaia, María Florencia
Volonteri, María Clara
Ceballos, Nora Raquel
author Regueira, Eleonora
author_facet Regueira, Eleonora
Sassone, Alina Grisel
Scaia, María Florencia
Volonteri, María Clara
Ceballos, Nora Raquel
author_role author
author2 Sassone, Alina Grisel
Scaia, María Florencia
Volonteri, María Clara
Ceballos, Nora Raquel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Toad
Glucocorticoids
Glucocorticoid Receptor
Androgens
topic Toad
Glucocorticoids
Glucocorticoid Receptor
Androgens
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Several studies indicate that wild free‐living vertebrates seasonally regulate plasma glucocorticoids. However, not only glucocorticoids but also the amount of receptors is important in determining biological responses. In this context, seasonal regulation of glucocorticoid receptor (GR) is crucial to modulate the response to glucocorticoids. Rhinella arenarum is an anuran exhibiting seasonal variations in plasma glucocorticoids and also in the number of binding sites (Bmax) of the testicular cytosolic GR. In this work, we evaluated if the annual pattern of GR protein in the testis varies seasonally and, by an in vitro approach, the role of glucocorticoids, androgens, and melatonin in the regulation of the GR Bmax and protein level. For this purpose, testes were treated with two physiological concentrations of melatonin (40 and 200 pg/ml), with or without luzindole (melatonin‐receptor antagonist); with testosterone, cyanoketone (inhibitor of steroidogenesis) or casodex (androgen‐receptor antagonist); or with dexamethasone or RU486 (GR antagonist). After treatments, Bmax and protein level were determined by the binding of [3 H]dexamethasone and Western blot, respectively. Results showed that GR protein decreases in the winter. The in vitro treatment with melatonin produced a biphasic effect on the Bmax with the lowest concentration decreasing this parameter by a receptor‐mediated mechanism. However, melatonin had no effect on the GR protein level. Conversely, a high concentration of dexamethasone up‐regulated the GR protein and androgens neither changed the Bmax nor the protein level. These findings suggest that seasonal changes in plasma melatonin and glucocorticoids modulate the effect of glucocorticoids in the testis of R. arenarum.
Fil: Regueira, Eleonora. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sassone, Alina Grisel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Scaia, María Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Volonteri, María Clara. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ceballos, Nora Raquel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Several studies indicate that wild free‐living vertebrates seasonally regulate plasma glucocorticoids. However, not only glucocorticoids but also the amount of receptors is important in determining biological responses. In this context, seasonal regulation of glucocorticoid receptor (GR) is crucial to modulate the response to glucocorticoids. Rhinella arenarum is an anuran exhibiting seasonal variations in plasma glucocorticoids and also in the number of binding sites (Bmax) of the testicular cytosolic GR. In this work, we evaluated if the annual pattern of GR protein in the testis varies seasonally and, by an in vitro approach, the role of glucocorticoids, androgens, and melatonin in the regulation of the GR Bmax and protein level. For this purpose, testes were treated with two physiological concentrations of melatonin (40 and 200 pg/ml), with or without luzindole (melatonin‐receptor antagonist); with testosterone, cyanoketone (inhibitor of steroidogenesis) or casodex (androgen‐receptor antagonist); or with dexamethasone or RU486 (GR antagonist). After treatments, Bmax and protein level were determined by the binding of [3 H]dexamethasone and Western blot, respectively. Results showed that GR protein decreases in the winter. The in vitro treatment with melatonin produced a biphasic effect on the Bmax with the lowest concentration decreasing this parameter by a receptor‐mediated mechanism. However, melatonin had no effect on the GR protein level. Conversely, a high concentration of dexamethasone up‐regulated the GR protein and androgens neither changed the Bmax nor the protein level. These findings suggest that seasonal changes in plasma melatonin and glucocorticoids modulate the effect of glucocorticoids in the testis of R. arenarum.
publishDate 2012
dc.date.none.fl_str_mv 2012-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/21301
Regueira, Eleonora; Sassone, Alina Grisel; Scaia, María Florencia; Volonteri, María Clara; Ceballos, Nora Raquel; Seasonal Changes and Regulation of the Glucocorticoid Receptor in the Testis of the Toad Rhinella arenarum; Wiley; Journal of Experimental Zoology Part A: Ecological Genetics and Physiology; 319; 1; 11-2012; 39-52
1932-5223
CONICET Digital
CONICET
url http://hdl.handle.net/11336/21301
identifier_str_mv Regueira, Eleonora; Sassone, Alina Grisel; Scaia, María Florencia; Volonteri, María Clara; Ceballos, Nora Raquel; Seasonal Changes and Regulation of the Glucocorticoid Receptor in the Testis of the Toad Rhinella arenarum; Wiley; Journal of Experimental Zoology Part A: Ecological Genetics and Physiology; 319; 1; 11-2012; 39-52
1932-5223
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/jez.1772
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jez.1772/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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