Indigo production by Pseudomonas sp. J26, a marine naphthalene-degrading strain

Autores
Riva Mercadal, Juan Pablo; Isaac, Paula; Siñeriz, Faustino; Ferrero, Marcela Alejandra
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A technique developed to determine naphthalene dioxygenase (NDO) activity was optimized and used to study the biotransformation of indole to indigo by Pseudomonas sp. J26 whole cells. The maximum production of indigo was achieved at 25 °C using 2.5 mM indole when J26 was grown in the complex medium JPP, while indole concentrations higher than 4 mM proved toxic for cells. The maximum rate of indigo production was 0.56 nmol min–1 mg dry biomass–1, obtaining 75.5 μM of indigo after 8 h of incubation, while a maximal concentration (138.1 μM) of indigo was obtained after 20 h.
Fil: Riva Mercadal, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Isaac, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Siñeriz, Faustino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ferrero, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Materia
Dioxigenasas
Indigo
Indol
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41506
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Indigo production by Pseudomonas sp. J26, a marine naphthalene-degrading strainRiva Mercadal, Juan PabloIsaac, PaulaSiñeriz, FaustinoFerrero, Marcela AlejandraDioxigenasasIndigoIndolhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2A technique developed to determine naphthalene dioxygenase (NDO) activity was optimized and used to study the biotransformation of indole to indigo by Pseudomonas sp. J26 whole cells. The maximum production of indigo was achieved at 25 °C using 2.5 mM indole when J26 was grown in the complex medium JPP, while indole concentrations higher than 4 mM proved toxic for cells. The maximum rate of indigo production was 0.56 nmol min–1 mg dry biomass–1, obtaining 75.5 μM of indigo after 8 h of incubation, while a maximal concentration (138.1 μM) of indigo was obtained after 20 h.Fil: Riva Mercadal, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Isaac, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Siñeriz, Faustino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Ferrero, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaWiley VCH Verlag2010-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41506Riva Mercadal, Juan Pablo; Isaac, Paula; Siñeriz, Faustino; Ferrero, Marcela Alejandra; Indigo production by Pseudomonas sp. J26, a marine naphthalene-degrading strain; Wiley VCH Verlag; Journal of Basic Microbiology; 50; 3; 5-2010; 290-2930233-111XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jobm.200900276info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jobm.200900276info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:02Zoai:ri.conicet.gov.ar:11336/41506instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:02.351CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Indigo production by Pseudomonas sp. J26, a marine naphthalene-degrading strain
title Indigo production by Pseudomonas sp. J26, a marine naphthalene-degrading strain
spellingShingle Indigo production by Pseudomonas sp. J26, a marine naphthalene-degrading strain
Riva Mercadal, Juan Pablo
Dioxigenasas
Indigo
Indol
title_short Indigo production by Pseudomonas sp. J26, a marine naphthalene-degrading strain
title_full Indigo production by Pseudomonas sp. J26, a marine naphthalene-degrading strain
title_fullStr Indigo production by Pseudomonas sp. J26, a marine naphthalene-degrading strain
title_full_unstemmed Indigo production by Pseudomonas sp. J26, a marine naphthalene-degrading strain
title_sort Indigo production by Pseudomonas sp. J26, a marine naphthalene-degrading strain
dc.creator.none.fl_str_mv Riva Mercadal, Juan Pablo
Isaac, Paula
Siñeriz, Faustino
Ferrero, Marcela Alejandra
author Riva Mercadal, Juan Pablo
author_facet Riva Mercadal, Juan Pablo
Isaac, Paula
Siñeriz, Faustino
Ferrero, Marcela Alejandra
author_role author
author2 Isaac, Paula
Siñeriz, Faustino
Ferrero, Marcela Alejandra
author2_role author
author
author
dc.subject.none.fl_str_mv Dioxigenasas
Indigo
Indol
topic Dioxigenasas
Indigo
Indol
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv A technique developed to determine naphthalene dioxygenase (NDO) activity was optimized and used to study the biotransformation of indole to indigo by Pseudomonas sp. J26 whole cells. The maximum production of indigo was achieved at 25 °C using 2.5 mM indole when J26 was grown in the complex medium JPP, while indole concentrations higher than 4 mM proved toxic for cells. The maximum rate of indigo production was 0.56 nmol min–1 mg dry biomass–1, obtaining 75.5 μM of indigo after 8 h of incubation, while a maximal concentration (138.1 μM) of indigo was obtained after 20 h.
Fil: Riva Mercadal, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Isaac, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Siñeriz, Faustino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ferrero, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
description A technique developed to determine naphthalene dioxygenase (NDO) activity was optimized and used to study the biotransformation of indole to indigo by Pseudomonas sp. J26 whole cells. The maximum production of indigo was achieved at 25 °C using 2.5 mM indole when J26 was grown in the complex medium JPP, while indole concentrations higher than 4 mM proved toxic for cells. The maximum rate of indigo production was 0.56 nmol min–1 mg dry biomass–1, obtaining 75.5 μM of indigo after 8 h of incubation, while a maximal concentration (138.1 μM) of indigo was obtained after 20 h.
publishDate 2010
dc.date.none.fl_str_mv 2010-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41506
Riva Mercadal, Juan Pablo; Isaac, Paula; Siñeriz, Faustino; Ferrero, Marcela Alejandra; Indigo production by Pseudomonas sp. J26, a marine naphthalene-degrading strain; Wiley VCH Verlag; Journal of Basic Microbiology; 50; 3; 5-2010; 290-293
0233-111X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41506
identifier_str_mv Riva Mercadal, Juan Pablo; Isaac, Paula; Siñeriz, Faustino; Ferrero, Marcela Alejandra; Indigo production by Pseudomonas sp. J26, a marine naphthalene-degrading strain; Wiley VCH Verlag; Journal of Basic Microbiology; 50; 3; 5-2010; 290-293
0233-111X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/jobm.200900276
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jobm.200900276
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270066866913280
score 13.13397

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