Phytoremediation of Phenol at pilot scale by tobacco hairy roots

Autores
Sosa Alderete, Lucas Gastón; Ibañez, Sabrina Guadalupe; Agostini, Elizabeth; Medina, Maria Ines
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Phenol is a toxic compound commonly found in the wastewater released from several industries. In the present work, we optimized the use of tobacco hairy roots (HRs), wild type (WT) and double transgenic (DT) for two peroxidases, as an alternative strategy for the treatment of this pollutant. The optimal conditions for the removal process were evaluated using different phenol (100-800 mg L-1) and H2O2 (2,5-10 mM) concentrations. Tobacco HRs were able to remove solutions with increasing phenol concentrations. Regarding the effect of exposition time, prolonged time of treatment improved phenol remediation when high pollutant concentrations were used. A decrease in basic peroxidase (Px) isoenzymes was detected after phenol treatment. Kinetic studies were performed using different Px extracts, such as soluble Px (SPx) and ionically bound Px (IBPx) from WT and DT HRs. SPx and IBPx extracts from DT HRs, showed high affinity towards phenol as substrate and higher catalytic efficiency than extracts derived from WT HRs. Moreover, IBPx extract from DT HRs, showed the highest catalytic efficiency, demonstrating that these HRs could provide an adequate matrix for the successful phenol removal.
Fil: Sosa Alderete, Lucas Gastón. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Ibañez, Sabrina Guadalupe. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Medina, Maria Ines. Universidad Nacional de Río Cuarto; Argentina
Materia
PHYTOREMEDIATION
ORGANIC POLLUTANTS
TRANSGENIC PEROXIDASES
KINETIC PARAMETERS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/229789
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Phytoremediation of Phenol at pilot scale by tobacco hairy rootsSosa Alderete, Lucas GastónIbañez, Sabrina GuadalupeAgostini, ElizabethMedina, Maria InesPHYTOREMEDIATIONORGANIC POLLUTANTSTRANSGENIC PEROXIDASESKINETIC PARAMETERShttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Phenol is a toxic compound commonly found in the wastewater released from several industries. In the present work, we optimized the use of tobacco hairy roots (HRs), wild type (WT) and double transgenic (DT) for two peroxidases, as an alternative strategy for the treatment of this pollutant. The optimal conditions for the removal process were evaluated using different phenol (100-800 mg L-1) and H2O2 (2,5-10 mM) concentrations. Tobacco HRs were able to remove solutions with increasing phenol concentrations. Regarding the effect of exposition time, prolonged time of treatment improved phenol remediation when high pollutant concentrations were used. A decrease in basic peroxidase (Px) isoenzymes was detected after phenol treatment. Kinetic studies were performed using different Px extracts, such as soluble Px (SPx) and ionically bound Px (IBPx) from WT and DT HRs. SPx and IBPx extracts from DT HRs, showed high affinity towards phenol as substrate and higher catalytic efficiency than extracts derived from WT HRs. Moreover, IBPx extract from DT HRs, showed the highest catalytic efficiency, demonstrating that these HRs could provide an adequate matrix for the successful phenol removal.Fil: Sosa Alderete, Lucas Gastón. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Ibañez, Sabrina Guadalupe. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Medina, Maria Ines. Universidad Nacional de Río Cuarto; ArgentinaIntegrated Publishing Association2012-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/229789Sosa Alderete, Lucas Gastón; Ibañez, Sabrina Guadalupe; Agostini, Elizabeth; Medina, Maria Ines; Phytoremediation of Phenol at pilot scale by tobacco hairy roots; Integrated Publishing Association; International Journal of Environmental Sciences; 3; 1; 7-2012; 398-4070976-4402CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.indianjournals.com/ijor.aspx?target=ijor:ijes&volume=3&issue=1&article=038&type=pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:43:16Zoai:ri.conicet.gov.ar:11336/229789instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:43:16.435CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phytoremediation of Phenol at pilot scale by tobacco hairy roots
title Phytoremediation of Phenol at pilot scale by tobacco hairy roots
spellingShingle Phytoremediation of Phenol at pilot scale by tobacco hairy roots
Sosa Alderete, Lucas Gastón
PHYTOREMEDIATION
ORGANIC POLLUTANTS
TRANSGENIC PEROXIDASES
KINETIC PARAMETERS
title_short Phytoremediation of Phenol at pilot scale by tobacco hairy roots
title_full Phytoremediation of Phenol at pilot scale by tobacco hairy roots
title_fullStr Phytoremediation of Phenol at pilot scale by tobacco hairy roots
title_full_unstemmed Phytoremediation of Phenol at pilot scale by tobacco hairy roots
title_sort Phytoremediation of Phenol at pilot scale by tobacco hairy roots
dc.creator.none.fl_str_mv Sosa Alderete, Lucas Gastón
Ibañez, Sabrina Guadalupe
Agostini, Elizabeth
Medina, Maria Ines
author Sosa Alderete, Lucas Gastón
author_facet Sosa Alderete, Lucas Gastón
Ibañez, Sabrina Guadalupe
Agostini, Elizabeth
Medina, Maria Ines
author_role author
author2 Ibañez, Sabrina Guadalupe
Agostini, Elizabeth
Medina, Maria Ines
author2_role author
author
author
dc.subject.none.fl_str_mv PHYTOREMEDIATION
ORGANIC POLLUTANTS
TRANSGENIC PEROXIDASES
KINETIC PARAMETERS
topic PHYTOREMEDIATION
ORGANIC POLLUTANTS
TRANSGENIC PEROXIDASES
KINETIC PARAMETERS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Phenol is a toxic compound commonly found in the wastewater released from several industries. In the present work, we optimized the use of tobacco hairy roots (HRs), wild type (WT) and double transgenic (DT) for two peroxidases, as an alternative strategy for the treatment of this pollutant. The optimal conditions for the removal process were evaluated using different phenol (100-800 mg L-1) and H2O2 (2,5-10 mM) concentrations. Tobacco HRs were able to remove solutions with increasing phenol concentrations. Regarding the effect of exposition time, prolonged time of treatment improved phenol remediation when high pollutant concentrations were used. A decrease in basic peroxidase (Px) isoenzymes was detected after phenol treatment. Kinetic studies were performed using different Px extracts, such as soluble Px (SPx) and ionically bound Px (IBPx) from WT and DT HRs. SPx and IBPx extracts from DT HRs, showed high affinity towards phenol as substrate and higher catalytic efficiency than extracts derived from WT HRs. Moreover, IBPx extract from DT HRs, showed the highest catalytic efficiency, demonstrating that these HRs could provide an adequate matrix for the successful phenol removal.
Fil: Sosa Alderete, Lucas Gastón. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Ibañez, Sabrina Guadalupe. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Medina, Maria Ines. Universidad Nacional de Río Cuarto; Argentina
description Phenol is a toxic compound commonly found in the wastewater released from several industries. In the present work, we optimized the use of tobacco hairy roots (HRs), wild type (WT) and double transgenic (DT) for two peroxidases, as an alternative strategy for the treatment of this pollutant. The optimal conditions for the removal process were evaluated using different phenol (100-800 mg L-1) and H2O2 (2,5-10 mM) concentrations. Tobacco HRs were able to remove solutions with increasing phenol concentrations. Regarding the effect of exposition time, prolonged time of treatment improved phenol remediation when high pollutant concentrations were used. A decrease in basic peroxidase (Px) isoenzymes was detected after phenol treatment. Kinetic studies were performed using different Px extracts, such as soluble Px (SPx) and ionically bound Px (IBPx) from WT and DT HRs. SPx and IBPx extracts from DT HRs, showed high affinity towards phenol as substrate and higher catalytic efficiency than extracts derived from WT HRs. Moreover, IBPx extract from DT HRs, showed the highest catalytic efficiency, demonstrating that these HRs could provide an adequate matrix for the successful phenol removal.
publishDate 2012
dc.date.none.fl_str_mv 2012-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/229789
Sosa Alderete, Lucas Gastón; Ibañez, Sabrina Guadalupe; Agostini, Elizabeth; Medina, Maria Ines; Phytoremediation of Phenol at pilot scale by tobacco hairy roots; Integrated Publishing Association; International Journal of Environmental Sciences; 3; 1; 7-2012; 398-407
0976-4402
CONICET Digital
CONICET
url http://hdl.handle.net/11336/229789
identifier_str_mv Sosa Alderete, Lucas Gastón; Ibañez, Sabrina Guadalupe; Agostini, Elizabeth; Medina, Maria Ines; Phytoremediation of Phenol at pilot scale by tobacco hairy roots; Integrated Publishing Association; International Journal of Environmental Sciences; 3; 1; 7-2012; 398-407
0976-4402
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.indianjournals.com/ijor.aspx?target=ijor:ijes&volume=3&issue=1&article=038&type=pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Integrated Publishing Association
publisher.none.fl_str_mv Integrated Publishing Association
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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