Development of Amine Capillary Column Applied to the Analysis of Basic Compounds by Electrochromatography

Autores
Flor, Sabrina Andrea; Huala, Juan Martin; Tripodi, Valeria Paula; Lucangioli, Silvia Edith
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An amine capillary column was developed in a fused-silica capillary as stationary phase and appliedto separation of basic compounds by capillary electrochromatography (CEC). The functionalizedcapillary was prepared by inmobilization of 3-Aminopropyl)triethoxysilane (APTES). TheCEC conditions including APTES and buffer concentration, pH and applied voltage were investigatedto obtain the optimal CEC system for the separation of three anesthetics as basic test molecules.The capillary column provided an efficiency of up to 20,000 plates/m. Lidocaine, ketamineand xilacine were baseline separated under the running conditions with 10 mM Na2HPO4 pH 9.0 asBGE. The applied voltage was 5 kV temperature was set at 25˚C and UV detection was performed.The resolutions were 4.97 and 1.53 for ketamine, lidocaine and xilacine, respectively. The columnused in CEC mode showed better separation of the anesthetics compared with those used in thecapillary zone mode. The comparison with reversed stationary phases used in HPLC in terms ofresolution (Rs), sensitivity (LOD), efficiency (N), precision (RSD), asymmetry (T) was also performed.This study provided an alternative way for the CEC separation of basic compounds anddemonstrated the improvement in the chromatographic parameters
Fil: Flor, Sabrina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: Huala, Juan Martin. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: Tripodi, Valeria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: Lucangioli, Silvia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Materia
ELECTROCHROMATOGRAPHY
BASIC COMPOUNDS
FUNZIONALIZED CAPILLARIES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47859
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/47859
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of Amine Capillary Column Applied to the Analysis of Basic Compounds by ElectrochromatographyFlor, Sabrina AndreaHuala, Juan MartinTripodi, Valeria PaulaLucangioli, Silvia EdithELECTROCHROMATOGRAPHYBASIC COMPOUNDSFUNZIONALIZED CAPILLARIEShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1An amine capillary column was developed in a fused-silica capillary as stationary phase and appliedto separation of basic compounds by capillary electrochromatography (CEC). The functionalizedcapillary was prepared by inmobilization of 3-Aminopropyl)triethoxysilane (APTES). TheCEC conditions including APTES and buffer concentration, pH and applied voltage were investigatedto obtain the optimal CEC system for the separation of three anesthetics as basic test molecules.The capillary column provided an efficiency of up to 20,000 plates/m. Lidocaine, ketamineand xilacine were baseline separated under the running conditions with 10 mM Na2HPO4 pH 9.0 asBGE. The applied voltage was 5 kV temperature was set at 25˚C and UV detection was performed.The resolutions were 4.97 and 1.53 for ketamine, lidocaine and xilacine, respectively. The columnused in CEC mode showed better separation of the anesthetics compared with those used in thecapillary zone mode. The comparison with reversed stationary phases used in HPLC in terms ofresolution (Rs), sensitivity (LOD), efficiency (N), precision (RSD), asymmetry (T) was also performed.This study provided an alternative way for the CEC separation of basic compounds anddemonstrated the improvement in the chromatographic parametersFil: Flor, Sabrina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; ArgentinaFil: Huala, Juan Martin. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; ArgentinaFil: Tripodi, Valeria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; ArgentinaFil: Lucangioli, Silvia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; ArgentinaScientific Research2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47859Flor, Sabrina Andrea; Huala, Juan Martin; Tripodi, Valeria Paula; Lucangioli, Silvia Edith; Development of Amine Capillary Column Applied to the Analysis of Basic Compounds by Electrochromatography; Scientific Research; Journal of Analytical Sciences, Methods and Instrumentation; 05; 04; 12-2015; 66-732164-2753CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4236/jasmi.2015.54008info:eu-repo/semantics/altIdentifier/url/http://file.scirp.org/Html/3-1000184_62253.htminfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:01:55Zoai:ri.conicet.gov.ar:11336/47859instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:01:55.872CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of Amine Capillary Column Applied to the Analysis of Basic Compounds by Electrochromatography
title Development of Amine Capillary Column Applied to the Analysis of Basic Compounds by Electrochromatography
spellingShingle Development of Amine Capillary Column Applied to the Analysis of Basic Compounds by Electrochromatography
Flor, Sabrina Andrea
ELECTROCHROMATOGRAPHY
BASIC COMPOUNDS
FUNZIONALIZED CAPILLARIES
title_short Development of Amine Capillary Column Applied to the Analysis of Basic Compounds by Electrochromatography
title_full Development of Amine Capillary Column Applied to the Analysis of Basic Compounds by Electrochromatography
title_fullStr Development of Amine Capillary Column Applied to the Analysis of Basic Compounds by Electrochromatography
title_full_unstemmed Development of Amine Capillary Column Applied to the Analysis of Basic Compounds by Electrochromatography
title_sort Development of Amine Capillary Column Applied to the Analysis of Basic Compounds by Electrochromatography
dc.creator.none.fl_str_mv Flor, Sabrina Andrea
Huala, Juan Martin
Tripodi, Valeria Paula
Lucangioli, Silvia Edith
author Flor, Sabrina Andrea
author_facet Flor, Sabrina Andrea
Huala, Juan Martin
Tripodi, Valeria Paula
Lucangioli, Silvia Edith
author_role author
author2 Huala, Juan Martin
Tripodi, Valeria Paula
Lucangioli, Silvia Edith
author2_role author
author
author
dc.subject.none.fl_str_mv ELECTROCHROMATOGRAPHY
BASIC COMPOUNDS
FUNZIONALIZED CAPILLARIES
topic ELECTROCHROMATOGRAPHY
BASIC COMPOUNDS
FUNZIONALIZED CAPILLARIES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv An amine capillary column was developed in a fused-silica capillary as stationary phase and appliedto separation of basic compounds by capillary electrochromatography (CEC). The functionalizedcapillary was prepared by inmobilization of 3-Aminopropyl)triethoxysilane (APTES). TheCEC conditions including APTES and buffer concentration, pH and applied voltage were investigatedto obtain the optimal CEC system for the separation of three anesthetics as basic test molecules.The capillary column provided an efficiency of up to 20,000 plates/m. Lidocaine, ketamineand xilacine were baseline separated under the running conditions with 10 mM Na2HPO4 pH 9.0 asBGE. The applied voltage was 5 kV temperature was set at 25˚C and UV detection was performed.The resolutions were 4.97 and 1.53 for ketamine, lidocaine and xilacine, respectively. The columnused in CEC mode showed better separation of the anesthetics compared with those used in thecapillary zone mode. The comparison with reversed stationary phases used in HPLC in terms ofresolution (Rs), sensitivity (LOD), efficiency (N), precision (RSD), asymmetry (T) was also performed.This study provided an alternative way for the CEC separation of basic compounds anddemonstrated the improvement in the chromatographic parameters
Fil: Flor, Sabrina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: Huala, Juan Martin. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: Tripodi, Valeria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: Lucangioli, Silvia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
description An amine capillary column was developed in a fused-silica capillary as stationary phase and appliedto separation of basic compounds by capillary electrochromatography (CEC). The functionalizedcapillary was prepared by inmobilization of 3-Aminopropyl)triethoxysilane (APTES). TheCEC conditions including APTES and buffer concentration, pH and applied voltage were investigatedto obtain the optimal CEC system for the separation of three anesthetics as basic test molecules.The capillary column provided an efficiency of up to 20,000 plates/m. Lidocaine, ketamineand xilacine were baseline separated under the running conditions with 10 mM Na2HPO4 pH 9.0 asBGE. The applied voltage was 5 kV temperature was set at 25˚C and UV detection was performed.The resolutions were 4.97 and 1.53 for ketamine, lidocaine and xilacine, respectively. The columnused in CEC mode showed better separation of the anesthetics compared with those used in thecapillary zone mode. The comparison with reversed stationary phases used in HPLC in terms ofresolution (Rs), sensitivity (LOD), efficiency (N), precision (RSD), asymmetry (T) was also performed.This study provided an alternative way for the CEC separation of basic compounds anddemonstrated the improvement in the chromatographic parameters
publishDate 2015
dc.date.none.fl_str_mv 2015-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47859
Flor, Sabrina Andrea; Huala, Juan Martin; Tripodi, Valeria Paula; Lucangioli, Silvia Edith; Development of Amine Capillary Column Applied to the Analysis of Basic Compounds by Electrochromatography; Scientific Research; Journal of Analytical Sciences, Methods and Instrumentation; 05; 04; 12-2015; 66-73
2164-2753
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47859
identifier_str_mv Flor, Sabrina Andrea; Huala, Juan Martin; Tripodi, Valeria Paula; Lucangioli, Silvia Edith; Development of Amine Capillary Column Applied to the Analysis of Basic Compounds by Electrochromatography; Scientific Research; Journal of Analytical Sciences, Methods and Instrumentation; 05; 04; 12-2015; 66-73
2164-2753
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4236/jasmi.2015.54008
info:eu-repo/semantics/altIdentifier/url/http://file.scirp.org/Html/3-1000184_62253.htm
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Scientific Research
publisher.none.fl_str_mv Scientific Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782353355046912
score 12.982451

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