First report of Colletotrichum siamense causing apple bitter rot in central Argentina

Autores
Fernandez, Laura Noemí; Alaniz, Sandra; Mondino, Pedro; Roeschlin, Roxana Andrea; Maumary, Roxana Lorena; Gariglio, Norberto Francisco; Favaro, María Alejandra
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The introduction of low-chill apple cultivars in Argentina allowed the expansion of production into warmer northern and central areas. Bitter rot, caused by Colletotrichum species, is one of the prevalent apple diseases worldwide (Baroncelli et al. 2014, Munir et al. 2016, Velho et al. 2015). In December 2014, bitter rot symptoms were observed on apple fruits cvs. ?Eva?, ?Caricia? and ?Princesa? in four orchards of Santa Fe province. The rot began as brown, 1-2 mm circular spots which enlarged rapidly and became sunken and extended toward the fruit core in a V-shaped pattern. Under high humidity conditions concentric rings of pinhead-size salmon acervuli formed in the lesions. Causal agent was isolated by touching acervuli with a sterile needle and monosporic cultures were obtained on PDA after 7 days at 25°C, with a 12-h light period. Colonies were white to gray on the top and pink on the underside, where concentric rings of salmon acervuli were clearly distinguished. The width and length of one hundred conidia were examined in three isolates (E3, E8 and E9), ranging from 3.37 to 5.54 μm (avg. 4.46), and from 11 to 17.85 μm (avg. 14.58), respectively. Conidia were mainly cylindrical, with rounded ends. After germination, conidia formed oval appressoria ranging from 9.17 to 10.31 μm (avg. 9.65), and from 6.88 to 7.81 μm (avg. 7.39). These morphological characteristics correspond to species belonging to C. gloesporioides complex (Weir et al. 2012). To accurately identify the species, DNA was extracted from isolates and genes corresponding to glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ß-tubulin (TUB2) and calmodulin (CAL) were partially amplified and sequenced. CAL and GAPDH sequences presented a 100% of identity with species of Colletotrichum siamense, whereas TUB2 sequences showed between 99 and 100 % of identity with the same species. The nucleotides sequences were deposited in GenBank (KY656675-KY656677, GAPDH; KY656678-KY656680, TUB2; and MF476801-MF476803, CAL). Multilocus phylogenetic analyses performed with references sequences (Weir et al. 2012) showed that the three isolates clustered with C. siamense, in accordance with BLAST results. To confirm pathogenicity, each isolate was inoculated in eight fruits of the cultivar from which it was originally obtained. Two drops of 10 μl of conidial suspension (1x105 conidia per ml) were deposited in wounded and non-wounded areas on fruits previously disinfested with 1% sodium hypochlorite solution for 1 min and rinsed twice with sterile distilled water. Drops of sterile water were deposited in eight fruits as control. Pathogenicity tests were repeated twice. Fruits were kept under high humidity conditions at 25°C for ten days. First symptoms appeared 3 days after inoculation (DAI) in wounded areas and 5 DAI in non-wounded areas. After that, all of the isolates produced symptoms identical to those previously described, whereas the controls remain symptomless. The pathogen was re-isolated from lesions, and identified as C. siamense by morphological characteristics and based on the CAL sequences, as previously described. To our knowledge, this is the first report of C. siamense in Argentina causing bitter rot on apple. C. siamense was previously reported to be more aggressive than other Colletotrichum species, but it is also more sensitive to fungicides (Munir et al. 2016), which encourages the development of species-specific management strategies for this pathogen in central Argentina.
Fil: Fernandez, Laura Noemí. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Alaniz, Sandra. Universidad de la Republica. Facultad de Agricultura; Uruguay
Fil: Mondino, Pedro. Universidad de la Republica. Facultad de Agricultura; Uruguay
Fil: Roeschlin, Roxana Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Reconquista; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Maumary, Roxana Lorena. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Gariglio, Norberto Francisco. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina
Fil: Favaro, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina
Materia
APPLE
BITTER ROT
MULTILOCUS PHYLOGENETIC ANALYSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/91309

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network_name_str CONICET Digital (CONICET)
spelling First report of Colletotrichum siamense causing apple bitter rot in central ArgentinaFernandez, Laura NoemíAlaniz, SandraMondino, PedroRoeschlin, Roxana AndreaMaumary, Roxana LorenaGariglio, Norberto FranciscoFavaro, María AlejandraAPPLEBITTER ROTMULTILOCUS PHYLOGENETIC ANALYSIShttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4The introduction of low-chill apple cultivars in Argentina allowed the expansion of production into warmer northern and central areas. Bitter rot, caused by Colletotrichum species, is one of the prevalent apple diseases worldwide (Baroncelli et al. 2014, Munir et al. 2016, Velho et al. 2015). In December 2014, bitter rot symptoms were observed on apple fruits cvs. ?Eva?, ?Caricia? and ?Princesa? in four orchards of Santa Fe province. The rot began as brown, 1-2 mm circular spots which enlarged rapidly and became sunken and extended toward the fruit core in a V-shaped pattern. Under high humidity conditions concentric rings of pinhead-size salmon acervuli formed in the lesions. Causal agent was isolated by touching acervuli with a sterile needle and monosporic cultures were obtained on PDA after 7 days at 25°C, with a 12-h light period. Colonies were white to gray on the top and pink on the underside, where concentric rings of salmon acervuli were clearly distinguished. The width and length of one hundred conidia were examined in three isolates (E3, E8 and E9), ranging from 3.37 to 5.54 μm (avg. 4.46), and from 11 to 17.85 μm (avg. 14.58), respectively. Conidia were mainly cylindrical, with rounded ends. After germination, conidia formed oval appressoria ranging from 9.17 to 10.31 μm (avg. 9.65), and from 6.88 to 7.81 μm (avg. 7.39). These morphological characteristics correspond to species belonging to C. gloesporioides complex (Weir et al. 2012). To accurately identify the species, DNA was extracted from isolates and genes corresponding to glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ß-tubulin (TUB2) and calmodulin (CAL) were partially amplified and sequenced. CAL and GAPDH sequences presented a 100% of identity with species of Colletotrichum siamense, whereas TUB2 sequences showed between 99 and 100 % of identity with the same species. The nucleotides sequences were deposited in GenBank (KY656675-KY656677, GAPDH; KY656678-KY656680, TUB2; and MF476801-MF476803, CAL). Multilocus phylogenetic analyses performed with references sequences (Weir et al. 2012) showed that the three isolates clustered with C. siamense, in accordance with BLAST results. To confirm pathogenicity, each isolate was inoculated in eight fruits of the cultivar from which it was originally obtained. Two drops of 10 μl of conidial suspension (1x105 conidia per ml) were deposited in wounded and non-wounded areas on fruits previously disinfested with 1% sodium hypochlorite solution for 1 min and rinsed twice with sterile distilled water. Drops of sterile water were deposited in eight fruits as control. Pathogenicity tests were repeated twice. Fruits were kept under high humidity conditions at 25°C for ten days. First symptoms appeared 3 days after inoculation (DAI) in wounded areas and 5 DAI in non-wounded areas. After that, all of the isolates produced symptoms identical to those previously described, whereas the controls remain symptomless. The pathogen was re-isolated from lesions, and identified as C. siamense by morphological characteristics and based on the CAL sequences, as previously described. To our knowledge, this is the first report of C. siamense in Argentina causing bitter rot on apple. C. siamense was previously reported to be more aggressive than other Colletotrichum species, but it is also more sensitive to fungicides (Munir et al. 2016), which encourages the development of species-specific management strategies for this pathogen in central Argentina.Fil: Fernandez, Laura Noemí. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Alaniz, Sandra. Universidad de la Republica. Facultad de Agricultura; UruguayFil: Mondino, Pedro. Universidad de la Republica. Facultad de Agricultura; UruguayFil: Roeschlin, Roxana Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Reconquista; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Maumary, Roxana Lorena. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Gariglio, Norberto Francisco. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; ArgentinaFil: Favaro, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; ArgentinaAmerican Phytopathological Society2018-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/91309Fernandez, Laura Noemí; Alaniz, Sandra; Mondino, Pedro; Roeschlin, Roxana Andrea; Maumary, Roxana Lorena; et al.; First report of Colletotrichum siamense causing apple bitter rot in central Argentina; American Phytopathological Society; Plant Disease; 102; 1; 1-2018; 1-20191-29171943-7692CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://apsjournals.apsnet.org/doi/full/10.1094/PDIS-07-17-1071-PDNinfo:eu-repo/semantics/altIdentifier/doi/10.1094/PDIS-07-17-1071-PDNinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:08Zoai:ri.conicet.gov.ar:11336/91309instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:08.894CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First report of Colletotrichum siamense causing apple bitter rot in central Argentina
title First report of Colletotrichum siamense causing apple bitter rot in central Argentina
spellingShingle First report of Colletotrichum siamense causing apple bitter rot in central Argentina
Fernandez, Laura Noemí
APPLE
BITTER ROT
MULTILOCUS PHYLOGENETIC ANALYSIS
title_short First report of Colletotrichum siamense causing apple bitter rot in central Argentina
title_full First report of Colletotrichum siamense causing apple bitter rot in central Argentina
title_fullStr First report of Colletotrichum siamense causing apple bitter rot in central Argentina
title_full_unstemmed First report of Colletotrichum siamense causing apple bitter rot in central Argentina
title_sort First report of Colletotrichum siamense causing apple bitter rot in central Argentina
dc.creator.none.fl_str_mv Fernandez, Laura Noemí
Alaniz, Sandra
Mondino, Pedro
Roeschlin, Roxana Andrea
Maumary, Roxana Lorena
Gariglio, Norberto Francisco
Favaro, María Alejandra
author Fernandez, Laura Noemí
author_facet Fernandez, Laura Noemí
Alaniz, Sandra
Mondino, Pedro
Roeschlin, Roxana Andrea
Maumary, Roxana Lorena
Gariglio, Norberto Francisco
Favaro, María Alejandra
author_role author
author2 Alaniz, Sandra
Mondino, Pedro
Roeschlin, Roxana Andrea
Maumary, Roxana Lorena
Gariglio, Norberto Francisco
Favaro, María Alejandra
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv APPLE
BITTER ROT
MULTILOCUS PHYLOGENETIC ANALYSIS
topic APPLE
BITTER ROT
MULTILOCUS PHYLOGENETIC ANALYSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The introduction of low-chill apple cultivars in Argentina allowed the expansion of production into warmer northern and central areas. Bitter rot, caused by Colletotrichum species, is one of the prevalent apple diseases worldwide (Baroncelli et al. 2014, Munir et al. 2016, Velho et al. 2015). In December 2014, bitter rot symptoms were observed on apple fruits cvs. ?Eva?, ?Caricia? and ?Princesa? in four orchards of Santa Fe province. The rot began as brown, 1-2 mm circular spots which enlarged rapidly and became sunken and extended toward the fruit core in a V-shaped pattern. Under high humidity conditions concentric rings of pinhead-size salmon acervuli formed in the lesions. Causal agent was isolated by touching acervuli with a sterile needle and monosporic cultures were obtained on PDA after 7 days at 25°C, with a 12-h light period. Colonies were white to gray on the top and pink on the underside, where concentric rings of salmon acervuli were clearly distinguished. The width and length of one hundred conidia were examined in three isolates (E3, E8 and E9), ranging from 3.37 to 5.54 μm (avg. 4.46), and from 11 to 17.85 μm (avg. 14.58), respectively. Conidia were mainly cylindrical, with rounded ends. After germination, conidia formed oval appressoria ranging from 9.17 to 10.31 μm (avg. 9.65), and from 6.88 to 7.81 μm (avg. 7.39). These morphological characteristics correspond to species belonging to C. gloesporioides complex (Weir et al. 2012). To accurately identify the species, DNA was extracted from isolates and genes corresponding to glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ß-tubulin (TUB2) and calmodulin (CAL) were partially amplified and sequenced. CAL and GAPDH sequences presented a 100% of identity with species of Colletotrichum siamense, whereas TUB2 sequences showed between 99 and 100 % of identity with the same species. The nucleotides sequences were deposited in GenBank (KY656675-KY656677, GAPDH; KY656678-KY656680, TUB2; and MF476801-MF476803, CAL). Multilocus phylogenetic analyses performed with references sequences (Weir et al. 2012) showed that the three isolates clustered with C. siamense, in accordance with BLAST results. To confirm pathogenicity, each isolate was inoculated in eight fruits of the cultivar from which it was originally obtained. Two drops of 10 μl of conidial suspension (1x105 conidia per ml) were deposited in wounded and non-wounded areas on fruits previously disinfested with 1% sodium hypochlorite solution for 1 min and rinsed twice with sterile distilled water. Drops of sterile water were deposited in eight fruits as control. Pathogenicity tests were repeated twice. Fruits were kept under high humidity conditions at 25°C for ten days. First symptoms appeared 3 days after inoculation (DAI) in wounded areas and 5 DAI in non-wounded areas. After that, all of the isolates produced symptoms identical to those previously described, whereas the controls remain symptomless. The pathogen was re-isolated from lesions, and identified as C. siamense by morphological characteristics and based on the CAL sequences, as previously described. To our knowledge, this is the first report of C. siamense in Argentina causing bitter rot on apple. C. siamense was previously reported to be more aggressive than other Colletotrichum species, but it is also more sensitive to fungicides (Munir et al. 2016), which encourages the development of species-specific management strategies for this pathogen in central Argentina.
Fil: Fernandez, Laura Noemí. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Alaniz, Sandra. Universidad de la Republica. Facultad de Agricultura; Uruguay
Fil: Mondino, Pedro. Universidad de la Republica. Facultad de Agricultura; Uruguay
Fil: Roeschlin, Roxana Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Reconquista; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Maumary, Roxana Lorena. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Gariglio, Norberto Francisco. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina
Fil: Favaro, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina
description The introduction of low-chill apple cultivars in Argentina allowed the expansion of production into warmer northern and central areas. Bitter rot, caused by Colletotrichum species, is one of the prevalent apple diseases worldwide (Baroncelli et al. 2014, Munir et al. 2016, Velho et al. 2015). In December 2014, bitter rot symptoms were observed on apple fruits cvs. ?Eva?, ?Caricia? and ?Princesa? in four orchards of Santa Fe province. The rot began as brown, 1-2 mm circular spots which enlarged rapidly and became sunken and extended toward the fruit core in a V-shaped pattern. Under high humidity conditions concentric rings of pinhead-size salmon acervuli formed in the lesions. Causal agent was isolated by touching acervuli with a sterile needle and monosporic cultures were obtained on PDA after 7 days at 25°C, with a 12-h light period. Colonies were white to gray on the top and pink on the underside, where concentric rings of salmon acervuli were clearly distinguished. The width and length of one hundred conidia were examined in three isolates (E3, E8 and E9), ranging from 3.37 to 5.54 μm (avg. 4.46), and from 11 to 17.85 μm (avg. 14.58), respectively. Conidia were mainly cylindrical, with rounded ends. After germination, conidia formed oval appressoria ranging from 9.17 to 10.31 μm (avg. 9.65), and from 6.88 to 7.81 μm (avg. 7.39). These morphological characteristics correspond to species belonging to C. gloesporioides complex (Weir et al. 2012). To accurately identify the species, DNA was extracted from isolates and genes corresponding to glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ß-tubulin (TUB2) and calmodulin (CAL) were partially amplified and sequenced. CAL and GAPDH sequences presented a 100% of identity with species of Colletotrichum siamense, whereas TUB2 sequences showed between 99 and 100 % of identity with the same species. The nucleotides sequences were deposited in GenBank (KY656675-KY656677, GAPDH; KY656678-KY656680, TUB2; and MF476801-MF476803, CAL). Multilocus phylogenetic analyses performed with references sequences (Weir et al. 2012) showed that the three isolates clustered with C. siamense, in accordance with BLAST results. To confirm pathogenicity, each isolate was inoculated in eight fruits of the cultivar from which it was originally obtained. Two drops of 10 μl of conidial suspension (1x105 conidia per ml) were deposited in wounded and non-wounded areas on fruits previously disinfested with 1% sodium hypochlorite solution for 1 min and rinsed twice with sterile distilled water. Drops of sterile water were deposited in eight fruits as control. Pathogenicity tests were repeated twice. Fruits were kept under high humidity conditions at 25°C for ten days. First symptoms appeared 3 days after inoculation (DAI) in wounded areas and 5 DAI in non-wounded areas. After that, all of the isolates produced symptoms identical to those previously described, whereas the controls remain symptomless. The pathogen was re-isolated from lesions, and identified as C. siamense by morphological characteristics and based on the CAL sequences, as previously described. To our knowledge, this is the first report of C. siamense in Argentina causing bitter rot on apple. C. siamense was previously reported to be more aggressive than other Colletotrichum species, but it is also more sensitive to fungicides (Munir et al. 2016), which encourages the development of species-specific management strategies for this pathogen in central Argentina.
publishDate 2018
dc.date.none.fl_str_mv 2018-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/91309
Fernandez, Laura Noemí; Alaniz, Sandra; Mondino, Pedro; Roeschlin, Roxana Andrea; Maumary, Roxana Lorena; et al.; First report of Colletotrichum siamense causing apple bitter rot in central Argentina; American Phytopathological Society; Plant Disease; 102; 1; 1-2018; 1-2
0191-2917
1943-7692
CONICET Digital
CONICET
url http://hdl.handle.net/11336/91309
identifier_str_mv Fernandez, Laura Noemí; Alaniz, Sandra; Mondino, Pedro; Roeschlin, Roxana Andrea; Maumary, Roxana Lorena; et al.; First report of Colletotrichum siamense causing apple bitter rot in central Argentina; American Phytopathological Society; Plant Disease; 102; 1; 1-2018; 1-2
0191-2917
1943-7692
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv American Phytopathological Society
publisher.none.fl_str_mv American Phytopathological Society
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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