First report of Colletotrichum siamense and C. fructicola causing cladode brown spot in Nopalea cochenillifera in Brazil

Autores
Conforto, C.; Bernardi Lima, Nelson; Garcete-Gómez, J. M.; Câmara, M. P. S.; Michereff, S. J.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In 2015, cladodes of Nopalea cochenillifera showing brown and circular to elliptical spots were sampled from 12 fields of the State of Pernambuco, Brazil with a prevalence of 100%. Small pieces of symptomatic tissues were surface sterilized, plated onto potato dextrose agar, and kept at 28°C for 7 days. Four isolates (CMM 2118, CMM 2166, CMM 2207, CMM 2228), produced white colonies, reverse pale yellow, conidia, hyaline, fusiform, 10.4-18.4 × 2.8-4.3 μm (n = 50). One isolate (CMM 2159) presented white colonies, becoming grey at the centre, conidia hyaline, cylindrical, 11.13-17.3 × 3.9-5.4 μm (n = 50). These morphological characteristics are consistent with the descriptions of C. siamense and C. fructicola, respectively (Prihastuti et al., 2009). For molecular identification, the ITS, TUB-2 and ApMAT loci were amplified, sequenced and analyzed using Bayesian inference, including published ITS, TUB-2 and ApMAT data for Colletotrichum spp. (Sharma et al., 2014). The isolated fungi grouped with the C. siamense and C. fructicola clades. Sequences of the isolates were deposited in GenBank (ITS, KX129708 to KX129712; TUB-2, KX129713 to KX129717; and ApMAT, KX129718 to KX129722). Pathogenicity tests with all five isolates of Colletotrichum were conducted on 15 healthy superficially sterilized detached cladodes per isolate. Scalpel-wounded and unwounded cladodes were inoculated with 2 × 105 conidial suspensions. Untreated controls were inoculated with sterile water. Cladodes were kept in a humid chamber for 72 h at 25°C in the dark. Fifteen days after inoculation all cladodes showed brown spots, with an average length of 1.1 cm and depth of 0.5 cm. Control cladodes remained symptomless. To our knowledge, this is the first report of C. fructicola and C. siamense causing cladode brown spot in Nopalea cochenillifera in Brazil and worldwide.
Fil: Conforto, C.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba; Argentina
Fil: Bernardi Lima, Nelson. Conicet - Instituto Nacional de Tecnologia Agropecuaria; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Garcete-Gómez, J. M.. Universidad Federal Rural Pernambuco; Brasil
Fil: Câmara, M. P. S.. Universidad Federal Rural Pernambuco; Brasil
Fil: Michereff, S. J.. Universidad Federal Rural Pernambuco; Brasil
Materia
Nopalea Cochenillefera
Colletotrichum
Brazil
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/72913
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/72913
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First report of Colletotrichum siamense and C. fructicola causing cladode brown spot in Nopalea cochenillifera in BrazilConforto, C.Bernardi Lima, NelsonGarcete-Gómez, J. M.Câmara, M. P. S.Michereff, S. J.Nopalea CochenilleferaColletotrichumBrazilhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4In 2015, cladodes of Nopalea cochenillifera showing brown and circular to elliptical spots were sampled from 12 fields of the State of Pernambuco, Brazil with a prevalence of 100%. Small pieces of symptomatic tissues were surface sterilized, plated onto potato dextrose agar, and kept at 28°C for 7 days. Four isolates (CMM 2118, CMM 2166, CMM 2207, CMM 2228), produced white colonies, reverse pale yellow, conidia, hyaline, fusiform, 10.4-18.4 × 2.8-4.3 μm (n = 50). One isolate (CMM 2159) presented white colonies, becoming grey at the centre, conidia hyaline, cylindrical, 11.13-17.3 × 3.9-5.4 μm (n = 50). These morphological characteristics are consistent with the descriptions of C. siamense and C. fructicola, respectively (Prihastuti et al., 2009). For molecular identification, the ITS, TUB-2 and ApMAT loci were amplified, sequenced and analyzed using Bayesian inference, including published ITS, TUB-2 and ApMAT data for Colletotrichum spp. (Sharma et al., 2014). The isolated fungi grouped with the C. siamense and C. fructicola clades. Sequences of the isolates were deposited in GenBank (ITS, KX129708 to KX129712; TUB-2, KX129713 to KX129717; and ApMAT, KX129718 to KX129722). Pathogenicity tests with all five isolates of Colletotrichum were conducted on 15 healthy superficially sterilized detached cladodes per isolate. Scalpel-wounded and unwounded cladodes were inoculated with 2 × 105 conidial suspensions. Untreated controls were inoculated with sterile water. Cladodes were kept in a humid chamber for 72 h at 25°C in the dark. Fifteen days after inoculation all cladodes showed brown spots, with an average length of 1.1 cm and depth of 0.5 cm. Control cladodes remained symptomless. To our knowledge, this is the first report of C. fructicola and C. siamense causing cladode brown spot in Nopalea cochenillifera in Brazil and worldwide.Fil: Conforto, C.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba; ArgentinaFil: Bernardi Lima, Nelson. Conicet - Instituto Nacional de Tecnologia Agropecuaria; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Garcete-Gómez, J. M.. Universidad Federal Rural Pernambuco; BrasilFil: Câmara, M. P. S.. Universidad Federal Rural Pernambuco; BrasilFil: Michereff, S. J.. Universidad Federal Rural Pernambuco; BrasilEdizioni Ets2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/72913Conforto, C. ; Bernardi Lima, Nelson; Garcete-Gómez, J. M.; Câmara, M. P. S.; Michereff, S. J.; First report of Colletotrichum siamense and C. fructicola causing cladode brown spot in Nopalea cochenillifera in Brazil; Edizioni Ets; Journal Of Plant Pathology; 99; 3; 11-20171125-4653CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sipav.org/main/jpp/index.php/jpp/article/view/3974info:eu-repo/semantics/altIdentifier/doi/10.4454/jpp.v99i3.3974info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:45:03Zoai:ri.conicet.gov.ar:11336/72913instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:45:03.59CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First report of Colletotrichum siamense and C. fructicola causing cladode brown spot in Nopalea cochenillifera in Brazil
title First report of Colletotrichum siamense and C. fructicola causing cladode brown spot in Nopalea cochenillifera in Brazil
spellingShingle First report of Colletotrichum siamense and C. fructicola causing cladode brown spot in Nopalea cochenillifera in Brazil
Conforto, C.
Nopalea Cochenillefera
Colletotrichum
Brazil
title_short First report of Colletotrichum siamense and C. fructicola causing cladode brown spot in Nopalea cochenillifera in Brazil
title_full First report of Colletotrichum siamense and C. fructicola causing cladode brown spot in Nopalea cochenillifera in Brazil
title_fullStr First report of Colletotrichum siamense and C. fructicola causing cladode brown spot in Nopalea cochenillifera in Brazil
title_full_unstemmed First report of Colletotrichum siamense and C. fructicola causing cladode brown spot in Nopalea cochenillifera in Brazil
title_sort First report of Colletotrichum siamense and C. fructicola causing cladode brown spot in Nopalea cochenillifera in Brazil
dc.creator.none.fl_str_mv Conforto, C.
Bernardi Lima, Nelson
Garcete-Gómez, J. M.
Câmara, M. P. S.
Michereff, S. J.
author Conforto, C.
author_facet Conforto, C.
Bernardi Lima, Nelson
Garcete-Gómez, J. M.
Câmara, M. P. S.
Michereff, S. J.
author_role author
author2 Bernardi Lima, Nelson
Garcete-Gómez, J. M.
Câmara, M. P. S.
Michereff, S. J.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Nopalea Cochenillefera
Colletotrichum
Brazil
topic Nopalea Cochenillefera
Colletotrichum
Brazil
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv In 2015, cladodes of Nopalea cochenillifera showing brown and circular to elliptical spots were sampled from 12 fields of the State of Pernambuco, Brazil with a prevalence of 100%. Small pieces of symptomatic tissues were surface sterilized, plated onto potato dextrose agar, and kept at 28°C for 7 days. Four isolates (CMM 2118, CMM 2166, CMM 2207, CMM 2228), produced white colonies, reverse pale yellow, conidia, hyaline, fusiform, 10.4-18.4 × 2.8-4.3 μm (n = 50). One isolate (CMM 2159) presented white colonies, becoming grey at the centre, conidia hyaline, cylindrical, 11.13-17.3 × 3.9-5.4 μm (n = 50). These morphological characteristics are consistent with the descriptions of C. siamense and C. fructicola, respectively (Prihastuti et al., 2009). For molecular identification, the ITS, TUB-2 and ApMAT loci were amplified, sequenced and analyzed using Bayesian inference, including published ITS, TUB-2 and ApMAT data for Colletotrichum spp. (Sharma et al., 2014). The isolated fungi grouped with the C. siamense and C. fructicola clades. Sequences of the isolates were deposited in GenBank (ITS, KX129708 to KX129712; TUB-2, KX129713 to KX129717; and ApMAT, KX129718 to KX129722). Pathogenicity tests with all five isolates of Colletotrichum were conducted on 15 healthy superficially sterilized detached cladodes per isolate. Scalpel-wounded and unwounded cladodes were inoculated with 2 × 105 conidial suspensions. Untreated controls were inoculated with sterile water. Cladodes were kept in a humid chamber for 72 h at 25°C in the dark. Fifteen days after inoculation all cladodes showed brown spots, with an average length of 1.1 cm and depth of 0.5 cm. Control cladodes remained symptomless. To our knowledge, this is the first report of C. fructicola and C. siamense causing cladode brown spot in Nopalea cochenillifera in Brazil and worldwide.
Fil: Conforto, C.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba; Argentina
Fil: Bernardi Lima, Nelson. Conicet - Instituto Nacional de Tecnologia Agropecuaria; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Garcete-Gómez, J. M.. Universidad Federal Rural Pernambuco; Brasil
Fil: Câmara, M. P. S.. Universidad Federal Rural Pernambuco; Brasil
Fil: Michereff, S. J.. Universidad Federal Rural Pernambuco; Brasil
description In 2015, cladodes of Nopalea cochenillifera showing brown and circular to elliptical spots were sampled from 12 fields of the State of Pernambuco, Brazil with a prevalence of 100%. Small pieces of symptomatic tissues were surface sterilized, plated onto potato dextrose agar, and kept at 28°C for 7 days. Four isolates (CMM 2118, CMM 2166, CMM 2207, CMM 2228), produced white colonies, reverse pale yellow, conidia, hyaline, fusiform, 10.4-18.4 × 2.8-4.3 μm (n = 50). One isolate (CMM 2159) presented white colonies, becoming grey at the centre, conidia hyaline, cylindrical, 11.13-17.3 × 3.9-5.4 μm (n = 50). These morphological characteristics are consistent with the descriptions of C. siamense and C. fructicola, respectively (Prihastuti et al., 2009). For molecular identification, the ITS, TUB-2 and ApMAT loci were amplified, sequenced and analyzed using Bayesian inference, including published ITS, TUB-2 and ApMAT data for Colletotrichum spp. (Sharma et al., 2014). The isolated fungi grouped with the C. siamense and C. fructicola clades. Sequences of the isolates were deposited in GenBank (ITS, KX129708 to KX129712; TUB-2, KX129713 to KX129717; and ApMAT, KX129718 to KX129722). Pathogenicity tests with all five isolates of Colletotrichum were conducted on 15 healthy superficially sterilized detached cladodes per isolate. Scalpel-wounded and unwounded cladodes were inoculated with 2 × 105 conidial suspensions. Untreated controls were inoculated with sterile water. Cladodes were kept in a humid chamber for 72 h at 25°C in the dark. Fifteen days after inoculation all cladodes showed brown spots, with an average length of 1.1 cm and depth of 0.5 cm. Control cladodes remained symptomless. To our knowledge, this is the first report of C. fructicola and C. siamense causing cladode brown spot in Nopalea cochenillifera in Brazil and worldwide.
publishDate 2017
dc.date.none.fl_str_mv 2017-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/72913
Conforto, C. ; Bernardi Lima, Nelson; Garcete-Gómez, J. M.; Câmara, M. P. S.; Michereff, S. J.; First report of Colletotrichum siamense and C. fructicola causing cladode brown spot in Nopalea cochenillifera in Brazil; Edizioni Ets; Journal Of Plant Pathology; 99; 3; 11-2017
1125-4653
CONICET Digital
CONICET
url http://hdl.handle.net/11336/72913
identifier_str_mv Conforto, C. ; Bernardi Lima, Nelson; Garcete-Gómez, J. M.; Câmara, M. P. S.; Michereff, S. J.; First report of Colletotrichum siamense and C. fructicola causing cladode brown spot in Nopalea cochenillifera in Brazil; Edizioni Ets; Journal Of Plant Pathology; 99; 3; 11-2017
1125-4653
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sipav.org/main/jpp/index.php/jpp/article/view/3974
info:eu-repo/semantics/altIdentifier/doi/10.4454/jpp.v99i3.3974
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Edizioni Ets
publisher.none.fl_str_mv Edizioni Ets
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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