Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum
- Autores
- Durruty, Ignacio; Gonzalez, Jorge Froilan; Wolski, Erika Alejandra
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- This research was undertaken to develop tools that facilitate the industrial application of an immobilized loofah-fungi system to degrade Direct Black 22 (DB22) azo dye. In laboratory-scale tests, the DB22, and loofah as support, were used. Assays without loofah were used as a free-cells control. The use of natural carriers to facilitate adhesion and growth of the fungi has shown favorable results. The degradation rate of immobilized cells increased twice as compared to free-cells control. At day 5 the decolorization was almost complete, while without loofah the total decolorization took more than 10 days. After 10 days, the extent of growth was nine times higher for the immobilized assays in comparison with the control flask. In subsequent experiments decolorization of DB22 was proven in a bench-scale reactor. A previously developed kinetic model was validated during the process. The model validation over free-cells assays gives an average normalized root mean squared error (ANRMSE) of 0.1659. Recalibration steps allowed prediction of the degradation with immobilized cells, resulting in an ANRMSE of 0.1891. A new calibration of the model during the scaling-up process yielded an ANRMSE of 0.1136 for DB22. The results presented encourage the use of this modeling tool in industrial scale facilities.
Fil: Durruty, Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Ingeniería Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gonzalez, Jorge Froilan. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Ingeniería Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wolski, Erika Alejandra. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Ingeniería Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
Bench-Scale Bioreactor
Direct Black 22
Fungi Immobilization
Model Validation - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/56274
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Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenumDurruty, IgnacioGonzalez, Jorge FroilanWolski, Erika AlejandraBench-Scale BioreactorDirect Black 22Fungi ImmobilizationModel Validationhttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1This research was undertaken to develop tools that facilitate the industrial application of an immobilized loofah-fungi system to degrade Direct Black 22 (DB22) azo dye. In laboratory-scale tests, the DB22, and loofah as support, were used. Assays without loofah were used as a free-cells control. The use of natural carriers to facilitate adhesion and growth of the fungi has shown favorable results. The degradation rate of immobilized cells increased twice as compared to free-cells control. At day 5 the decolorization was almost complete, while without loofah the total decolorization took more than 10 days. After 10 days, the extent of growth was nine times higher for the immobilized assays in comparison with the control flask. In subsequent experiments decolorization of DB22 was proven in a bench-scale reactor. A previously developed kinetic model was validated during the process. The model validation over free-cells assays gives an average normalized root mean squared error (ANRMSE) of 0.1659. Recalibration steps allowed prediction of the degradation with immobilized cells, resulting in an ANRMSE of 0.1891. A new calibration of the model during the scaling-up process yielded an ANRMSE of 0.1136 for DB22. The results presented encourage the use of this modeling tool in industrial scale facilities.Fil: Durruty, Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Ingeniería Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gonzalez, Jorge Froilan. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Ingeniería Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Wolski, Erika Alejandra. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Ingeniería Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaIWA Publishing2018-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/56274Durruty, Ignacio; Gonzalez, Jorge Froilan; Wolski, Erika Alejandra; Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum; IWA Publishing; Water Science And Technology; 77; 1; 1-2018; 17-260273-1223CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.2166/wst.2017.514info:eu-repo/semantics/altIdentifier/url/https://iwaponline.com/wst/article-abstract/77/1/17/38434/Scaling-up-and-kinetic-model-validation-of-Directinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:43:41Zoai:ri.conicet.gov.ar:11336/56274instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:43:41.486CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum |
title |
Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum |
spellingShingle |
Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum Durruty, Ignacio Bench-Scale Bioreactor Direct Black 22 Fungi Immobilization Model Validation |
title_short |
Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum |
title_full |
Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum |
title_fullStr |
Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum |
title_full_unstemmed |
Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum |
title_sort |
Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum |
dc.creator.none.fl_str_mv |
Durruty, Ignacio Gonzalez, Jorge Froilan Wolski, Erika Alejandra |
author |
Durruty, Ignacio |
author_facet |
Durruty, Ignacio Gonzalez, Jorge Froilan Wolski, Erika Alejandra |
author_role |
author |
author2 |
Gonzalez, Jorge Froilan Wolski, Erika Alejandra |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Bench-Scale Bioreactor Direct Black 22 Fungi Immobilization Model Validation |
topic |
Bench-Scale Bioreactor Direct Black 22 Fungi Immobilization Model Validation |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.5 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
This research was undertaken to develop tools that facilitate the industrial application of an immobilized loofah-fungi system to degrade Direct Black 22 (DB22) azo dye. In laboratory-scale tests, the DB22, and loofah as support, were used. Assays without loofah were used as a free-cells control. The use of natural carriers to facilitate adhesion and growth of the fungi has shown favorable results. The degradation rate of immobilized cells increased twice as compared to free-cells control. At day 5 the decolorization was almost complete, while without loofah the total decolorization took more than 10 days. After 10 days, the extent of growth was nine times higher for the immobilized assays in comparison with the control flask. In subsequent experiments decolorization of DB22 was proven in a bench-scale reactor. A previously developed kinetic model was validated during the process. The model validation over free-cells assays gives an average normalized root mean squared error (ANRMSE) of 0.1659. Recalibration steps allowed prediction of the degradation with immobilized cells, resulting in an ANRMSE of 0.1891. A new calibration of the model during the scaling-up process yielded an ANRMSE of 0.1136 for DB22. The results presented encourage the use of this modeling tool in industrial scale facilities. Fil: Durruty, Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Ingeniería Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gonzalez, Jorge Froilan. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Ingeniería Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Wolski, Erika Alejandra. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Ingeniería Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
This research was undertaken to develop tools that facilitate the industrial application of an immobilized loofah-fungi system to degrade Direct Black 22 (DB22) azo dye. In laboratory-scale tests, the DB22, and loofah as support, were used. Assays without loofah were used as a free-cells control. The use of natural carriers to facilitate adhesion and growth of the fungi has shown favorable results. The degradation rate of immobilized cells increased twice as compared to free-cells control. At day 5 the decolorization was almost complete, while without loofah the total decolorization took more than 10 days. After 10 days, the extent of growth was nine times higher for the immobilized assays in comparison with the control flask. In subsequent experiments decolorization of DB22 was proven in a bench-scale reactor. A previously developed kinetic model was validated during the process. The model validation over free-cells assays gives an average normalized root mean squared error (ANRMSE) of 0.1659. Recalibration steps allowed prediction of the degradation with immobilized cells, resulting in an ANRMSE of 0.1891. A new calibration of the model during the scaling-up process yielded an ANRMSE of 0.1136 for DB22. The results presented encourage the use of this modeling tool in industrial scale facilities. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/56274 Durruty, Ignacio; Gonzalez, Jorge Froilan; Wolski, Erika Alejandra; Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum; IWA Publishing; Water Science And Technology; 77; 1; 1-2018; 17-26 0273-1223 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/56274 |
identifier_str_mv |
Durruty, Ignacio; Gonzalez, Jorge Froilan; Wolski, Erika Alejandra; Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum; IWA Publishing; Water Science And Technology; 77; 1; 1-2018; 17-26 0273-1223 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.2166/wst.2017.514 info:eu-repo/semantics/altIdentifier/url/https://iwaponline.com/wst/article-abstract/77/1/17/38434/Scaling-up-and-kinetic-model-validation-of-Direct |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
IWA Publishing |
publisher.none.fl_str_mv |
IWA Publishing |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614472869609472 |
score |
13.070432 |