Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum

Autores
Durruty, Ignacio; Gonzalez, Jorge Froilan; Wolski, Erika Alejandra
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This research was undertaken to develop tools that facilitate the industrial application of an immobilized loofah-fungi system to degrade Direct Black 22 (DB22) azo dye. In laboratory-scale tests, the DB22, and loofah as support, were used. Assays without loofah were used as a free-cells control. The use of natural carriers to facilitate adhesion and growth of the fungi has shown favorable results. The degradation rate of immobilized cells increased twice as compared to free-cells control. At day 5 the decolorization was almost complete, while without loofah the total decolorization took more than 10 days. After 10 days, the extent of growth was nine times higher for the immobilized assays in comparison with the control flask. In subsequent experiments decolorization of DB22 was proven in a bench-scale reactor. A previously developed kinetic model was validated during the process. The model validation over free-cells assays gives an average normalized root mean squared error (ANRMSE) of 0.1659. Recalibration steps allowed prediction of the degradation with immobilized cells, resulting in an ANRMSE of 0.1891. A new calibration of the model during the scaling-up process yielded an ANRMSE of 0.1136 for DB22. The results presented encourage the use of this modeling tool in industrial scale facilities.
Fil: Durruty, Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Ingeniería Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gonzalez, Jorge Froilan. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Ingeniería Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wolski, Erika Alejandra. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Ingeniería Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Bench-Scale Bioreactor
Direct Black 22
Fungi Immobilization
Model Validation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/56274
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenumDurruty, IgnacioGonzalez, Jorge FroilanWolski, Erika AlejandraBench-Scale BioreactorDirect Black 22Fungi ImmobilizationModel Validationhttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1This research was undertaken to develop tools that facilitate the industrial application of an immobilized loofah-fungi system to degrade Direct Black 22 (DB22) azo dye. In laboratory-scale tests, the DB22, and loofah as support, were used. Assays without loofah were used as a free-cells control. The use of natural carriers to facilitate adhesion and growth of the fungi has shown favorable results. The degradation rate of immobilized cells increased twice as compared to free-cells control. At day 5 the decolorization was almost complete, while without loofah the total decolorization took more than 10 days. After 10 days, the extent of growth was nine times higher for the immobilized assays in comparison with the control flask. In subsequent experiments decolorization of DB22 was proven in a bench-scale reactor. A previously developed kinetic model was validated during the process. The model validation over free-cells assays gives an average normalized root mean squared error (ANRMSE) of 0.1659. Recalibration steps allowed prediction of the degradation with immobilized cells, resulting in an ANRMSE of 0.1891. A new calibration of the model during the scaling-up process yielded an ANRMSE of 0.1136 for DB22. The results presented encourage the use of this modeling tool in industrial scale facilities.Fil: Durruty, Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Ingeniería Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gonzalez, Jorge Froilan. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Ingeniería Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Wolski, Erika Alejandra. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Ingeniería Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaIWA Publishing2018-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/56274Durruty, Ignacio; Gonzalez, Jorge Froilan; Wolski, Erika Alejandra; Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum; IWA Publishing; Water Science And Technology; 77; 1; 1-2018; 17-260273-1223CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.2166/wst.2017.514info:eu-repo/semantics/altIdentifier/url/https://iwaponline.com/wst/article-abstract/77/1/17/38434/Scaling-up-and-kinetic-model-validation-of-Directinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:16:37Zoai:ri.conicet.gov.ar:11336/56274instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:16:37.975CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum
title Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum
spellingShingle Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum
Durruty, Ignacio
Bench-Scale Bioreactor
Direct Black 22
Fungi Immobilization
Model Validation
title_short Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum
title_full Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum
title_fullStr Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum
title_full_unstemmed Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum
title_sort Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum
dc.creator.none.fl_str_mv Durruty, Ignacio
Gonzalez, Jorge Froilan
Wolski, Erika Alejandra
author Durruty, Ignacio
author_facet Durruty, Ignacio
Gonzalez, Jorge Froilan
Wolski, Erika Alejandra
author_role author
author2 Gonzalez, Jorge Froilan
Wolski, Erika Alejandra
author2_role author
author
dc.subject.none.fl_str_mv Bench-Scale Bioreactor
Direct Black 22
Fungi Immobilization
Model Validation
topic Bench-Scale Bioreactor
Direct Black 22
Fungi Immobilization
Model Validation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This research was undertaken to develop tools that facilitate the industrial application of an immobilized loofah-fungi system to degrade Direct Black 22 (DB22) azo dye. In laboratory-scale tests, the DB22, and loofah as support, were used. Assays without loofah were used as a free-cells control. The use of natural carriers to facilitate adhesion and growth of the fungi has shown favorable results. The degradation rate of immobilized cells increased twice as compared to free-cells control. At day 5 the decolorization was almost complete, while without loofah the total decolorization took more than 10 days. After 10 days, the extent of growth was nine times higher for the immobilized assays in comparison with the control flask. In subsequent experiments decolorization of DB22 was proven in a bench-scale reactor. A previously developed kinetic model was validated during the process. The model validation over free-cells assays gives an average normalized root mean squared error (ANRMSE) of 0.1659. Recalibration steps allowed prediction of the degradation with immobilized cells, resulting in an ANRMSE of 0.1891. A new calibration of the model during the scaling-up process yielded an ANRMSE of 0.1136 for DB22. The results presented encourage the use of this modeling tool in industrial scale facilities.
Fil: Durruty, Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Ingeniería Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gonzalez, Jorge Froilan. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Ingeniería Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wolski, Erika Alejandra. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Ingeniería Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description This research was undertaken to develop tools that facilitate the industrial application of an immobilized loofah-fungi system to degrade Direct Black 22 (DB22) azo dye. In laboratory-scale tests, the DB22, and loofah as support, were used. Assays without loofah were used as a free-cells control. The use of natural carriers to facilitate adhesion and growth of the fungi has shown favorable results. The degradation rate of immobilized cells increased twice as compared to free-cells control. At day 5 the decolorization was almost complete, while without loofah the total decolorization took more than 10 days. After 10 days, the extent of growth was nine times higher for the immobilized assays in comparison with the control flask. In subsequent experiments decolorization of DB22 was proven in a bench-scale reactor. A previously developed kinetic model was validated during the process. The model validation over free-cells assays gives an average normalized root mean squared error (ANRMSE) of 0.1659. Recalibration steps allowed prediction of the degradation with immobilized cells, resulting in an ANRMSE of 0.1891. A new calibration of the model during the scaling-up process yielded an ANRMSE of 0.1136 for DB22. The results presented encourage the use of this modeling tool in industrial scale facilities.
publishDate 2018
dc.date.none.fl_str_mv 2018-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/56274
Durruty, Ignacio; Gonzalez, Jorge Froilan; Wolski, Erika Alejandra; Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum; IWA Publishing; Water Science And Technology; 77; 1; 1-2018; 17-26
0273-1223
CONICET Digital
CONICET
url http://hdl.handle.net/11336/56274
identifier_str_mv Durruty, Ignacio; Gonzalez, Jorge Froilan; Wolski, Erika Alejandra; Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum; IWA Publishing; Water Science And Technology; 77; 1; 1-2018; 17-26
0273-1223
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.2166/wst.2017.514
info:eu-repo/semantics/altIdentifier/url/https://iwaponline.com/wst/article-abstract/77/1/17/38434/Scaling-up-and-kinetic-model-validation-of-Direct
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv IWA Publishing
publisher.none.fl_str_mv IWA Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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