Energetic reserves and digestive enzyme activities in juveniles of the red claw crayfish Cherax quadricarinatus nearby the point-of-no-return

Autores
Calvo, Natalia Soledad; Stumpf, Liane; Sacristán, Hernán Javier; Lopez, Laura Susana
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cherax quadricarinatus displays biological attributes that make it suitable for commercial aquaculture and, as a freshwater species, it has high starvation resistance. Previous studies demonstrated that after 40 days of starvation only the 25% (PNR25) and after 50 days the 50% (PNR50) of one-gram juveniles died. The objective of this study is to characterize the pattern of use of energetic reserves, through the analysis of digestive enzyme activities and the occurrence of lipids, proteins and glycogen in the hepatopancreas and pleon muscle, nearby PNR25, PNR50 and after a feeding period. One-gram juveniles were randomly assigned to one of two feeding protocols: continuous feeding throughout 90-day (Control) and starvation until day 50 and then feeding for the following 40 days (Treatment). Juveniles from each feeding protocol were weighed, measured and sacrificed at day 40, 50 or 90. Total lipids, glycogen and proteins were determined on hepatopancreas and pleon; and lipase, amylase and protease activities were also estimated in the hepatopancreas. Growth stopped during starvation and resumed when food was supplied. Close to the point-of-no-return 50 pleon muscle began to degrade diminishing protein content and the lipid content decrease significantly in the hepatopancreas. However, after the feeding period both reserves were completely replenished. Although glycogen levels were not affected during starvation, a pronounced accumulation of this nutrient in the pleon was triggered when food was available. The lipase activity decreased during starvation suggesting that the lipase whose activity was measured may not be synthesized when food is not available. Although starvation had no a significant effect on the protease and amylase activities, they tended to decrease around the point-of-no-return and to increase after the feeding period. In this context, the present research provides new and relevant biological information on physiological and biochemical responses of crustaceans nearby PNR25 and PNR50.
Fil: Calvo, Natalia Soledad. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biologia Experimental. Laboratorio de Biologia de la Reproduccion y Crecimiento de Crustaceos Decapodos; Argentina
Fil: Stumpf, Liane. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biologia Experimental. Laboratorio de Biologia de la Reproduccion y Crecimiento de Crustaceos Decapodos; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biologia Experimental y Aplicada; Argentina
Fil: Sacristán, Hernán Javier. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biologia Experimental. Laboratorio de Biologia de la Reproduccion y Crecimiento de Crustaceos Decapodos; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biologia Experimental y Aplicada; Argentina
Fil: Lopez, Laura Susana. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biologia Experimental. Laboratorio de Biologia de la Reproduccion y Crecimiento de Crustaceos Decapodos; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biologia Experimental y Aplicada; Argentina
Materia
Pnr
Lipid
Hepatopancreas
Pleon
Energetic Reserves
Digestive Enzymes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11547
Acceder
id CONICETDig_7366949aa834c117f3094ac48b6854d2
oai_identifier_str oai:ri.conicet.gov.ar:11336/11547
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Energetic reserves and digestive enzyme activities in juveniles of the red claw crayfish Cherax quadricarinatus nearby the point-of-no-returnCalvo, Natalia SoledadStumpf, LianeSacristán, Hernán JavierLopez, Laura SusanaPnrLipidHepatopancreasPleonEnergetic ReservesDigestive Enzymeshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cherax quadricarinatus displays biological attributes that make it suitable for commercial aquaculture and, as a freshwater species, it has high starvation resistance. Previous studies demonstrated that after 40 days of starvation only the 25% (PNR25) and after 50 days the 50% (PNR50) of one-gram juveniles died. The objective of this study is to characterize the pattern of use of energetic reserves, through the analysis of digestive enzyme activities and the occurrence of lipids, proteins and glycogen in the hepatopancreas and pleon muscle, nearby PNR25, PNR50 and after a feeding period. One-gram juveniles were randomly assigned to one of two feeding protocols: continuous feeding throughout 90-day (Control) and starvation until day 50 and then feeding for the following 40 days (Treatment). Juveniles from each feeding protocol were weighed, measured and sacrificed at day 40, 50 or 90. Total lipids, glycogen and proteins were determined on hepatopancreas and pleon; and lipase, amylase and protease activities were also estimated in the hepatopancreas. Growth stopped during starvation and resumed when food was supplied. Close to the point-of-no-return 50 pleon muscle began to degrade diminishing protein content and the lipid content decrease significantly in the hepatopancreas. However, after the feeding period both reserves were completely replenished. Although glycogen levels were not affected during starvation, a pronounced accumulation of this nutrient in the pleon was triggered when food was available. The lipase activity decreased during starvation suggesting that the lipase whose activity was measured may not be synthesized when food is not available. Although starvation had no a significant effect on the protease and amylase activities, they tended to decrease around the point-of-no-return and to increase after the feeding period. In this context, the present research provides new and relevant biological information on physiological and biochemical responses of crustaceans nearby PNR25 and PNR50.Fil: Calvo, Natalia Soledad. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biologia Experimental. Laboratorio de Biologia de la Reproduccion y Crecimiento de Crustaceos Decapodos; ArgentinaFil: Stumpf, Liane. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biologia Experimental. Laboratorio de Biologia de la Reproduccion y Crecimiento de Crustaceos Decapodos; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biologia Experimental y Aplicada; ArgentinaFil: Sacristán, Hernán Javier. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biologia Experimental. Laboratorio de Biologia de la Reproduccion y Crecimiento de Crustaceos Decapodos; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biologia Experimental y Aplicada; ArgentinaFil: Lopez, Laura Susana. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biologia Experimental. Laboratorio de Biologia de la Reproduccion y Crecimiento de Crustaceos Decapodos; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biologia Experimental y Aplicada; ArgentinaElsevier Science2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11547Calvo, Natalia Soledad; Stumpf, Liane; Sacristán, Hernán Javier; Lopez, Laura Susana; Energetic reserves and digestive enzyme activities in juveniles of the red claw crayfish Cherax quadricarinatus nearby the point-of-no-return; Elsevier Science; Aquaculture; 416-417; 8-2013; 85-910044-8486enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0044848613004158info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2013.08.017info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:45Zoai:ri.conicet.gov.ar:11336/11547instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:46.535CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Energetic reserves and digestive enzyme activities in juveniles of the red claw crayfish Cherax quadricarinatus nearby the point-of-no-return
title Energetic reserves and digestive enzyme activities in juveniles of the red claw crayfish Cherax quadricarinatus nearby the point-of-no-return
spellingShingle Energetic reserves and digestive enzyme activities in juveniles of the red claw crayfish Cherax quadricarinatus nearby the point-of-no-return
Calvo, Natalia Soledad
Pnr
Lipid
Hepatopancreas
Pleon
Energetic Reserves
Digestive Enzymes
title_short Energetic reserves and digestive enzyme activities in juveniles of the red claw crayfish Cherax quadricarinatus nearby the point-of-no-return
title_full Energetic reserves and digestive enzyme activities in juveniles of the red claw crayfish Cherax quadricarinatus nearby the point-of-no-return
title_fullStr Energetic reserves and digestive enzyme activities in juveniles of the red claw crayfish Cherax quadricarinatus nearby the point-of-no-return
title_full_unstemmed Energetic reserves and digestive enzyme activities in juveniles of the red claw crayfish Cherax quadricarinatus nearby the point-of-no-return
title_sort Energetic reserves and digestive enzyme activities in juveniles of the red claw crayfish Cherax quadricarinatus nearby the point-of-no-return
dc.creator.none.fl_str_mv Calvo, Natalia Soledad
Stumpf, Liane
Sacristán, Hernán Javier
Lopez, Laura Susana
author Calvo, Natalia Soledad
author_facet Calvo, Natalia Soledad
Stumpf, Liane
Sacristán, Hernán Javier
Lopez, Laura Susana
author_role author
author2 Stumpf, Liane
Sacristán, Hernán Javier
Lopez, Laura Susana
author2_role author
author
author
dc.subject.none.fl_str_mv Pnr
Lipid
Hepatopancreas
Pleon
Energetic Reserves
Digestive Enzymes
topic Pnr
Lipid
Hepatopancreas
Pleon
Energetic Reserves
Digestive Enzymes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cherax quadricarinatus displays biological attributes that make it suitable for commercial aquaculture and, as a freshwater species, it has high starvation resistance. Previous studies demonstrated that after 40 days of starvation only the 25% (PNR25) and after 50 days the 50% (PNR50) of one-gram juveniles died. The objective of this study is to characterize the pattern of use of energetic reserves, through the analysis of digestive enzyme activities and the occurrence of lipids, proteins and glycogen in the hepatopancreas and pleon muscle, nearby PNR25, PNR50 and after a feeding period. One-gram juveniles were randomly assigned to one of two feeding protocols: continuous feeding throughout 90-day (Control) and starvation until day 50 and then feeding for the following 40 days (Treatment). Juveniles from each feeding protocol were weighed, measured and sacrificed at day 40, 50 or 90. Total lipids, glycogen and proteins were determined on hepatopancreas and pleon; and lipase, amylase and protease activities were also estimated in the hepatopancreas. Growth stopped during starvation and resumed when food was supplied. Close to the point-of-no-return 50 pleon muscle began to degrade diminishing protein content and the lipid content decrease significantly in the hepatopancreas. However, after the feeding period both reserves were completely replenished. Although glycogen levels were not affected during starvation, a pronounced accumulation of this nutrient in the pleon was triggered when food was available. The lipase activity decreased during starvation suggesting that the lipase whose activity was measured may not be synthesized when food is not available. Although starvation had no a significant effect on the protease and amylase activities, they tended to decrease around the point-of-no-return and to increase after the feeding period. In this context, the present research provides new and relevant biological information on physiological and biochemical responses of crustaceans nearby PNR25 and PNR50.
Fil: Calvo, Natalia Soledad. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biologia Experimental. Laboratorio de Biologia de la Reproduccion y Crecimiento de Crustaceos Decapodos; Argentina
Fil: Stumpf, Liane. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biologia Experimental. Laboratorio de Biologia de la Reproduccion y Crecimiento de Crustaceos Decapodos; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biologia Experimental y Aplicada; Argentina
Fil: Sacristán, Hernán Javier. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biologia Experimental. Laboratorio de Biologia de la Reproduccion y Crecimiento de Crustaceos Decapodos; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biologia Experimental y Aplicada; Argentina
Fil: Lopez, Laura Susana. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biologia Experimental. Laboratorio de Biologia de la Reproduccion y Crecimiento de Crustaceos Decapodos; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biologia Experimental y Aplicada; Argentina
description Cherax quadricarinatus displays biological attributes that make it suitable for commercial aquaculture and, as a freshwater species, it has high starvation resistance. Previous studies demonstrated that after 40 days of starvation only the 25% (PNR25) and after 50 days the 50% (PNR50) of one-gram juveniles died. The objective of this study is to characterize the pattern of use of energetic reserves, through the analysis of digestive enzyme activities and the occurrence of lipids, proteins and glycogen in the hepatopancreas and pleon muscle, nearby PNR25, PNR50 and after a feeding period. One-gram juveniles were randomly assigned to one of two feeding protocols: continuous feeding throughout 90-day (Control) and starvation until day 50 and then feeding for the following 40 days (Treatment). Juveniles from each feeding protocol were weighed, measured and sacrificed at day 40, 50 or 90. Total lipids, glycogen and proteins were determined on hepatopancreas and pleon; and lipase, amylase and protease activities were also estimated in the hepatopancreas. Growth stopped during starvation and resumed when food was supplied. Close to the point-of-no-return 50 pleon muscle began to degrade diminishing protein content and the lipid content decrease significantly in the hepatopancreas. However, after the feeding period both reserves were completely replenished. Although glycogen levels were not affected during starvation, a pronounced accumulation of this nutrient in the pleon was triggered when food was available. The lipase activity decreased during starvation suggesting that the lipase whose activity was measured may not be synthesized when food is not available. Although starvation had no a significant effect on the protease and amylase activities, they tended to decrease around the point-of-no-return and to increase after the feeding period. In this context, the present research provides new and relevant biological information on physiological and biochemical responses of crustaceans nearby PNR25 and PNR50.
publishDate 2013
dc.date.none.fl_str_mv 2013-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11547
Calvo, Natalia Soledad; Stumpf, Liane; Sacristán, Hernán Javier; Lopez, Laura Susana; Energetic reserves and digestive enzyme activities in juveniles of the red claw crayfish Cherax quadricarinatus nearby the point-of-no-return; Elsevier Science; Aquaculture; 416-417; 8-2013; 85-91
0044-8486
url http://hdl.handle.net/11336/11547
identifier_str_mv Calvo, Natalia Soledad; Stumpf, Liane; Sacristán, Hernán Javier; Lopez, Laura Susana; Energetic reserves and digestive enzyme activities in juveniles of the red claw crayfish Cherax quadricarinatus nearby the point-of-no-return; Elsevier Science; Aquaculture; 416-417; 8-2013; 85-91
0044-8486
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0044848613004158
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2013.08.017
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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