Digestive flexibility in a euryhaline crab from a SW Atlantic coastal lagoon: Alkaline phosphatase activity sensitive to salinity in the hepatopancreas

Autores
Pinoni, Silvina Andrea; Méndez, Eugenia; Lopez Mañanes, Alejandra Antonia
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We studied biochemical characteristics and the response to low salinity at short and long-term after feeding of alkaline phosphatase (AP) activity in hepatopancreas of the osmoregulator crab Neohelice granulata from Mar Chiquita coastal lagoon (Buenos Aires Province, Argentina) (37°32′-37°45′S 57°19′-57°26′W). The hepatopancreas exhibited a levamisole-insensitive and a levamisole-sensitive AP activity with distinct characteristics. Levamisole-insensitive activity was similar within the range of pH 7.4-9.0 and exhibited a Michaelis-Menten kinetics. Levamisole-sensitive AP activity appeared to be maximal at pH 8.5 and appeared to exhibit an allosteric kinetics. In crabs acclimated to 10 psu (hyper-regulation conditions) levamisole-insensitive and levamisole-sensitive AP activity increased (about 16-fold) over time from short term (2-4 h) to long term (120 h) after feeding while no changes occurred in crabs acclimated to 35 psu (osmoconforming conditions). The changes of AP activity along with the higher values at 120 h after feeding in 10 psu compared with those in 35 psu, and the concomitant changes in proteolytic activity, suggest a role of AP in digestive and metabolic adjustments at the biochemical level upon hyper-regulatory conditions.
Fil: Pinoni, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Méndez, Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Lopez Mañanes, Alejandra Antonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Materia
Alkaline Phosphatase
Crabs
Digestive Flexibility
Hepatopancreas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/50164
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/50164
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Digestive flexibility in a euryhaline crab from a SW Atlantic coastal lagoon: Alkaline phosphatase activity sensitive to salinity in the hepatopancreasPinoni, Silvina AndreaMéndez, EugeniaLopez Mañanes, Alejandra AntoniaAlkaline PhosphataseCrabsDigestive FlexibilityHepatopancreashttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We studied biochemical characteristics and the response to low salinity at short and long-term after feeding of alkaline phosphatase (AP) activity in hepatopancreas of the osmoregulator crab Neohelice granulata from Mar Chiquita coastal lagoon (Buenos Aires Province, Argentina) (37°32′-37°45′S 57°19′-57°26′W). The hepatopancreas exhibited a levamisole-insensitive and a levamisole-sensitive AP activity with distinct characteristics. Levamisole-insensitive activity was similar within the range of pH 7.4-9.0 and exhibited a Michaelis-Menten kinetics. Levamisole-sensitive AP activity appeared to be maximal at pH 8.5 and appeared to exhibit an allosteric kinetics. In crabs acclimated to 10 psu (hyper-regulation conditions) levamisole-insensitive and levamisole-sensitive AP activity increased (about 16-fold) over time from short term (2-4 h) to long term (120 h) after feeding while no changes occurred in crabs acclimated to 35 psu (osmoconforming conditions). The changes of AP activity along with the higher values at 120 h after feeding in 10 psu compared with those in 35 psu, and the concomitant changes in proteolytic activity, suggest a role of AP in digestive and metabolic adjustments at the biochemical level upon hyper-regulatory conditions.Fil: Pinoni, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Méndez, Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Lopez Mañanes, Alejandra Antonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaCambridge University Press2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/50164Pinoni, Silvina Andrea; Méndez, Eugenia; Lopez Mañanes, Alejandra Antonia; Digestive flexibility in a euryhaline crab from a SW Atlantic coastal lagoon: Alkaline phosphatase activity sensitive to salinity in the hepatopancreas; Cambridge University Press; Journal of the Marine Biological Association of the United Kingdom; 95; 6; 9-2015; 1133-11400025-3154CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1017/S0025315415000570info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/journal-of-the-marine-biological-association-of-the-united-kingdom/article/digestive-flexibility-in-a-euryhaline-crab-from-a-sw-atlantic-coastal-lagoon-alkaline-phosphatase-activity-sensitive-to-salinity-in-the-hepatopancreas/73CBF3014201B19FB28155DF5814917Dinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:06Zoai:ri.conicet.gov.ar:11336/50164instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:07.199CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Digestive flexibility in a euryhaline crab from a SW Atlantic coastal lagoon: Alkaline phosphatase activity sensitive to salinity in the hepatopancreas
title Digestive flexibility in a euryhaline crab from a SW Atlantic coastal lagoon: Alkaline phosphatase activity sensitive to salinity in the hepatopancreas
spellingShingle Digestive flexibility in a euryhaline crab from a SW Atlantic coastal lagoon: Alkaline phosphatase activity sensitive to salinity in the hepatopancreas
Pinoni, Silvina Andrea
Alkaline Phosphatase
Crabs
Digestive Flexibility
Hepatopancreas
title_short Digestive flexibility in a euryhaline crab from a SW Atlantic coastal lagoon: Alkaline phosphatase activity sensitive to salinity in the hepatopancreas
title_full Digestive flexibility in a euryhaline crab from a SW Atlantic coastal lagoon: Alkaline phosphatase activity sensitive to salinity in the hepatopancreas
title_fullStr Digestive flexibility in a euryhaline crab from a SW Atlantic coastal lagoon: Alkaline phosphatase activity sensitive to salinity in the hepatopancreas
title_full_unstemmed Digestive flexibility in a euryhaline crab from a SW Atlantic coastal lagoon: Alkaline phosphatase activity sensitive to salinity in the hepatopancreas
title_sort Digestive flexibility in a euryhaline crab from a SW Atlantic coastal lagoon: Alkaline phosphatase activity sensitive to salinity in the hepatopancreas
dc.creator.none.fl_str_mv Pinoni, Silvina Andrea
Méndez, Eugenia
Lopez Mañanes, Alejandra Antonia
author Pinoni, Silvina Andrea
author_facet Pinoni, Silvina Andrea
Méndez, Eugenia
Lopez Mañanes, Alejandra Antonia
author_role author
author2 Méndez, Eugenia
Lopez Mañanes, Alejandra Antonia
author2_role author
author
dc.subject.none.fl_str_mv Alkaline Phosphatase
Crabs
Digestive Flexibility
Hepatopancreas
topic Alkaline Phosphatase
Crabs
Digestive Flexibility
Hepatopancreas
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We studied biochemical characteristics and the response to low salinity at short and long-term after feeding of alkaline phosphatase (AP) activity in hepatopancreas of the osmoregulator crab Neohelice granulata from Mar Chiquita coastal lagoon (Buenos Aires Province, Argentina) (37°32′-37°45′S 57°19′-57°26′W). The hepatopancreas exhibited a levamisole-insensitive and a levamisole-sensitive AP activity with distinct characteristics. Levamisole-insensitive activity was similar within the range of pH 7.4-9.0 and exhibited a Michaelis-Menten kinetics. Levamisole-sensitive AP activity appeared to be maximal at pH 8.5 and appeared to exhibit an allosteric kinetics. In crabs acclimated to 10 psu (hyper-regulation conditions) levamisole-insensitive and levamisole-sensitive AP activity increased (about 16-fold) over time from short term (2-4 h) to long term (120 h) after feeding while no changes occurred in crabs acclimated to 35 psu (osmoconforming conditions). The changes of AP activity along with the higher values at 120 h after feeding in 10 psu compared with those in 35 psu, and the concomitant changes in proteolytic activity, suggest a role of AP in digestive and metabolic adjustments at the biochemical level upon hyper-regulatory conditions.
Fil: Pinoni, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Méndez, Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Lopez Mañanes, Alejandra Antonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
description We studied biochemical characteristics and the response to low salinity at short and long-term after feeding of alkaline phosphatase (AP) activity in hepatopancreas of the osmoregulator crab Neohelice granulata from Mar Chiquita coastal lagoon (Buenos Aires Province, Argentina) (37°32′-37°45′S 57°19′-57°26′W). The hepatopancreas exhibited a levamisole-insensitive and a levamisole-sensitive AP activity with distinct characteristics. Levamisole-insensitive activity was similar within the range of pH 7.4-9.0 and exhibited a Michaelis-Menten kinetics. Levamisole-sensitive AP activity appeared to be maximal at pH 8.5 and appeared to exhibit an allosteric kinetics. In crabs acclimated to 10 psu (hyper-regulation conditions) levamisole-insensitive and levamisole-sensitive AP activity increased (about 16-fold) over time from short term (2-4 h) to long term (120 h) after feeding while no changes occurred in crabs acclimated to 35 psu (osmoconforming conditions). The changes of AP activity along with the higher values at 120 h after feeding in 10 psu compared with those in 35 psu, and the concomitant changes in proteolytic activity, suggest a role of AP in digestive and metabolic adjustments at the biochemical level upon hyper-regulatory conditions.
publishDate 2015
dc.date.none.fl_str_mv 2015-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/50164
Pinoni, Silvina Andrea; Méndez, Eugenia; Lopez Mañanes, Alejandra Antonia; Digestive flexibility in a euryhaline crab from a SW Atlantic coastal lagoon: Alkaline phosphatase activity sensitive to salinity in the hepatopancreas; Cambridge University Press; Journal of the Marine Biological Association of the United Kingdom; 95; 6; 9-2015; 1133-1140
0025-3154
CONICET Digital
CONICET
url http://hdl.handle.net/11336/50164
identifier_str_mv Pinoni, Silvina Andrea; Méndez, Eugenia; Lopez Mañanes, Alejandra Antonia; Digestive flexibility in a euryhaline crab from a SW Atlantic coastal lagoon: Alkaline phosphatase activity sensitive to salinity in the hepatopancreas; Cambridge University Press; Journal of the Marine Biological Association of the United Kingdom; 95; 6; 9-2015; 1133-1140
0025-3154
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1017/S0025315415000570
info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/journal-of-the-marine-biological-association-of-the-united-kingdom/article/digestive-flexibility-in-a-euryhaline-crab-from-a-sw-atlantic-coastal-lagoon-alkaline-phosphatase-activity-sensitive-to-salinity-in-the-hepatopancreas/73CBF3014201B19FB28155DF5814917D
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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