Galectin-1 Markedly Reduces the Incidence of Resorptions in Mice Missing Immunophilin FKBP52

Autores
Hirota, Yasushi; Burnum, Kristin E.; Acar, Nuray; Rabinovich, Gabriel Adrián; Daikoku, Takiko; Dey, Sudhansu K.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Progesterone (P4) signaling is critical for pregnancy.We previously showed that immunopilin FK506 binding protein (FKBP)52 serves as a cochaperone to optimize progesterone receptor (PR) function in the uterus, and its deficiency leads to P4 resistance in a pregnancy stage-specific and genetic background-dependent manner in mice. In particular, sc placement of SILASTIC implants carrying P4 rescued implantation failure in CD1 Fkbp52 / mice, but the resorption rate was substantially high at midgestation due to reduced P4 responsiveness. Because downstream targets of P4- FKBP52-PR signaling in the uterus to support pregnancy are not clearly understood, we performed proteomic analysis using Fkbp52 / , PR-deficient (Pgr / ), and wild-type (WT) uteri.We found that the expression of galectin-1 (Gal1), an evolutionarily conserved glycan-binding protein, was significantly down-regulated in both Fkbp52 / and Pgr / uteri compared with WT uteri. During early gestation, Lgals1, which encodes Gal1, was distinctly expressed in stromal and decidual cells. Lgals1 expression was much lower in d 4 Fkbp52 / uteri compared with WT uteri, and this reduction was reversed by P4 supplementation. More interestingly, concomitant supplementation of recombinant Gal1 significantly suppressed the high resorption rate and leukocyte infiltration at implantation sites in CD1 Fkbp52 / females carrying P4 SILASTIC implants. These findings suggest that uterine Gal1 is an important downstream target of P4-FKBP52-PR signaling in the uterus to support P4 responsiveness during pregnancy.
Fil: Hirota, Yasushi. University of Tokyo; Japón
Fil: Burnum, Kristin E.. Vanderbilt University; Estados Unidos
Fil: Acar, Nuray. Cincinnati Children's Hospital Medical Center; Estados Unidos
Fil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Daikoku, Takiko. Cincinnati Children's Hospital Medical Center; Estados Unidos
Fil: Dey, Sudhansu K.. Cincinnati Children's Hospital Medical Center; Estados Unidos
Materia
Tacrolimus Binding Proteins
Pregnancy
Galectin-1
Immunophilins
Signal Traduction
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/22248
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/22248
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Galectin-1 Markedly Reduces the Incidence of Resorptions in Mice Missing Immunophilin FKBP52Hirota, YasushiBurnum, Kristin E.Acar, NurayRabinovich, Gabriel AdriánDaikoku, TakikoDey, Sudhansu K.Tacrolimus Binding ProteinsPregnancyGalectin-1ImmunophilinsSignal Traductionhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Progesterone (P4) signaling is critical for pregnancy.We previously showed that immunopilin FK506 binding protein (FKBP)52 serves as a cochaperone to optimize progesterone receptor (PR) function in the uterus, and its deficiency leads to P4 resistance in a pregnancy stage-specific and genetic background-dependent manner in mice. In particular, sc placement of SILASTIC implants carrying P4 rescued implantation failure in CD1 Fkbp52 / mice, but the resorption rate was substantially high at midgestation due to reduced P4 responsiveness. Because downstream targets of P4- FKBP52-PR signaling in the uterus to support pregnancy are not clearly understood, we performed proteomic analysis using Fkbp52 / , PR-deficient (Pgr / ), and wild-type (WT) uteri.We found that the expression of galectin-1 (Gal1), an evolutionarily conserved glycan-binding protein, was significantly down-regulated in both Fkbp52 / and Pgr / uteri compared with WT uteri. During early gestation, Lgals1, which encodes Gal1, was distinctly expressed in stromal and decidual cells. Lgals1 expression was much lower in d 4 Fkbp52 / uteri compared with WT uteri, and this reduction was reversed by P4 supplementation. More interestingly, concomitant supplementation of recombinant Gal1 significantly suppressed the high resorption rate and leukocyte infiltration at implantation sites in CD1 Fkbp52 / females carrying P4 SILASTIC implants. These findings suggest that uterine Gal1 is an important downstream target of P4-FKBP52-PR signaling in the uterus to support P4 responsiveness during pregnancy.Fil: Hirota, Yasushi. University of Tokyo; JapónFil: Burnum, Kristin E.. Vanderbilt University; Estados UnidosFil: Acar, Nuray. Cincinnati Children's Hospital Medical Center; Estados UnidosFil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Daikoku, Takiko. Cincinnati Children's Hospital Medical Center; Estados UnidosFil: Dey, Sudhansu K.. Cincinnati Children's Hospital Medical Center; Estados UnidosOxford University Press2012-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22248Hirota, Yasushi; Burnum, Kristin E.; Acar, Nuray; Rabinovich, Gabriel Adrián; Daikoku, Takiko; et al.; Galectin-1 Markedly Reduces the Incidence of Resorptions in Mice Missing Immunophilin FKBP52; Oxford University Press; Endocrinology; 153; 5; 5-2012; 2486-24930013-72271945-7170CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/endo/article-lookup/doi/10.1210/en.2012-1035info:eu-repo/semantics/altIdentifier/doi/10.1210/en.2012-1035info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3339653/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:31Zoai:ri.conicet.gov.ar:11336/22248instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:31.281CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Galectin-1 Markedly Reduces the Incidence of Resorptions in Mice Missing Immunophilin FKBP52
title Galectin-1 Markedly Reduces the Incidence of Resorptions in Mice Missing Immunophilin FKBP52
spellingShingle Galectin-1 Markedly Reduces the Incidence of Resorptions in Mice Missing Immunophilin FKBP52
Hirota, Yasushi
Tacrolimus Binding Proteins
Pregnancy
Galectin-1
Immunophilins
Signal Traduction
title_short Galectin-1 Markedly Reduces the Incidence of Resorptions in Mice Missing Immunophilin FKBP52
title_full Galectin-1 Markedly Reduces the Incidence of Resorptions in Mice Missing Immunophilin FKBP52
title_fullStr Galectin-1 Markedly Reduces the Incidence of Resorptions in Mice Missing Immunophilin FKBP52
title_full_unstemmed Galectin-1 Markedly Reduces the Incidence of Resorptions in Mice Missing Immunophilin FKBP52
title_sort Galectin-1 Markedly Reduces the Incidence of Resorptions in Mice Missing Immunophilin FKBP52
dc.creator.none.fl_str_mv Hirota, Yasushi
Burnum, Kristin E.
Acar, Nuray
Rabinovich, Gabriel Adrián
Daikoku, Takiko
Dey, Sudhansu K.
author Hirota, Yasushi
author_facet Hirota, Yasushi
Burnum, Kristin E.
Acar, Nuray
Rabinovich, Gabriel Adrián
Daikoku, Takiko
Dey, Sudhansu K.
author_role author
author2 Burnum, Kristin E.
Acar, Nuray
Rabinovich, Gabriel Adrián
Daikoku, Takiko
Dey, Sudhansu K.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Tacrolimus Binding Proteins
Pregnancy
Galectin-1
Immunophilins
Signal Traduction
topic Tacrolimus Binding Proteins
Pregnancy
Galectin-1
Immunophilins
Signal Traduction
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Progesterone (P4) signaling is critical for pregnancy.We previously showed that immunopilin FK506 binding protein (FKBP)52 serves as a cochaperone to optimize progesterone receptor (PR) function in the uterus, and its deficiency leads to P4 resistance in a pregnancy stage-specific and genetic background-dependent manner in mice. In particular, sc placement of SILASTIC implants carrying P4 rescued implantation failure in CD1 Fkbp52 / mice, but the resorption rate was substantially high at midgestation due to reduced P4 responsiveness. Because downstream targets of P4- FKBP52-PR signaling in the uterus to support pregnancy are not clearly understood, we performed proteomic analysis using Fkbp52 / , PR-deficient (Pgr / ), and wild-type (WT) uteri.We found that the expression of galectin-1 (Gal1), an evolutionarily conserved glycan-binding protein, was significantly down-regulated in both Fkbp52 / and Pgr / uteri compared with WT uteri. During early gestation, Lgals1, which encodes Gal1, was distinctly expressed in stromal and decidual cells. Lgals1 expression was much lower in d 4 Fkbp52 / uteri compared with WT uteri, and this reduction was reversed by P4 supplementation. More interestingly, concomitant supplementation of recombinant Gal1 significantly suppressed the high resorption rate and leukocyte infiltration at implantation sites in CD1 Fkbp52 / females carrying P4 SILASTIC implants. These findings suggest that uterine Gal1 is an important downstream target of P4-FKBP52-PR signaling in the uterus to support P4 responsiveness during pregnancy.
Fil: Hirota, Yasushi. University of Tokyo; Japón
Fil: Burnum, Kristin E.. Vanderbilt University; Estados Unidos
Fil: Acar, Nuray. Cincinnati Children's Hospital Medical Center; Estados Unidos
Fil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Daikoku, Takiko. Cincinnati Children's Hospital Medical Center; Estados Unidos
Fil: Dey, Sudhansu K.. Cincinnati Children's Hospital Medical Center; Estados Unidos
description Progesterone (P4) signaling is critical for pregnancy.We previously showed that immunopilin FK506 binding protein (FKBP)52 serves as a cochaperone to optimize progesterone receptor (PR) function in the uterus, and its deficiency leads to P4 resistance in a pregnancy stage-specific and genetic background-dependent manner in mice. In particular, sc placement of SILASTIC implants carrying P4 rescued implantation failure in CD1 Fkbp52 / mice, but the resorption rate was substantially high at midgestation due to reduced P4 responsiveness. Because downstream targets of P4- FKBP52-PR signaling in the uterus to support pregnancy are not clearly understood, we performed proteomic analysis using Fkbp52 / , PR-deficient (Pgr / ), and wild-type (WT) uteri.We found that the expression of galectin-1 (Gal1), an evolutionarily conserved glycan-binding protein, was significantly down-regulated in both Fkbp52 / and Pgr / uteri compared with WT uteri. During early gestation, Lgals1, which encodes Gal1, was distinctly expressed in stromal and decidual cells. Lgals1 expression was much lower in d 4 Fkbp52 / uteri compared with WT uteri, and this reduction was reversed by P4 supplementation. More interestingly, concomitant supplementation of recombinant Gal1 significantly suppressed the high resorption rate and leukocyte infiltration at implantation sites in CD1 Fkbp52 / females carrying P4 SILASTIC implants. These findings suggest that uterine Gal1 is an important downstream target of P4-FKBP52-PR signaling in the uterus to support P4 responsiveness during pregnancy.
publishDate 2012
dc.date.none.fl_str_mv 2012-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22248
Hirota, Yasushi; Burnum, Kristin E.; Acar, Nuray; Rabinovich, Gabriel Adrián; Daikoku, Takiko; et al.; Galectin-1 Markedly Reduces the Incidence of Resorptions in Mice Missing Immunophilin FKBP52; Oxford University Press; Endocrinology; 153; 5; 5-2012; 2486-2493
0013-7227
1945-7170
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22248
identifier_str_mv Hirota, Yasushi; Burnum, Kristin E.; Acar, Nuray; Rabinovich, Gabriel Adrián; Daikoku, Takiko; et al.; Galectin-1 Markedly Reduces the Incidence of Resorptions in Mice Missing Immunophilin FKBP52; Oxford University Press; Endocrinology; 153; 5; 5-2012; 2486-2493
0013-7227
1945-7170
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/endo/article-lookup/doi/10.1210/en.2012-1035
info:eu-repo/semantics/altIdentifier/doi/10.1210/en.2012-1035
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3339653/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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