Tunneling Nanotube-like Structures in Giardia duodenalis

Autores
Midlej, Victor; Tenaglia, Albano Heraldo; Luján, Hugo D.; de Souza, Wanderley
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Giardia doudenalis (lamblia, intestinalis) is a protozoan parasite that inhabits the lumen of the upper small intestine of vertebrates, causing chronic abdominal pains and severe diarrhea, symptoms of giardiasis, a persistent and recurrent infection. This characteristic is mainly due to the presence of membrane variant-specific surface proteins (VSPs) that give this parasite the ability to successively infect the host through antigenic variation. Using high-resolution scanning microscopy (HR-SM), we observed the presence, formation, and extension of tunneling-nanotube-like surface structures in Giardia, especially following parasite challenges with VSP antibodies. They were seen all over the parasite surface, both in vitro and in vivo, showing that G. duodenalis nanotube formation occurs in complex environments such as the gut. In addition, we also observed that some of these nanotubes displayed a periodic strangulation that produces 100 nm vesicles that seemed to be released in a process similar to that previously observed in Trypanosoma brucei. The presence of nanotube-like structures in G. duodenalis highlights yet another strategy of cellular communication utilized by these parasites, whether between themselves or with the host cell.
Fil: Midlej, Victor. Instituto Oswaldo Cruz ; Fundacion Oswaldo Cruz ; Ministerio de Salud de Brasil;
Fil: Tenaglia, Albano Heraldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Luján, Hugo D.. Johannes Gutenberg Universitat Mainz; Alemania
Fil: de Souza, Wanderley. Universidade Federal Do Rio de Janeiro. Centro Nacional de Biologia Estrutural E Bioimagem.; Brasil
Materia
PROTOZOAN CELL
ULTRASTRUCTURE
TUNNELING NANOTUBES
HIGH-RESOLUTION MICROSCOPY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/257090
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Tunneling Nanotube-like Structures in Giardia duodenalisMidlej, VictorTenaglia, Albano HeraldoLuján, Hugo D.de Souza, WanderleyPROTOZOAN CELLULTRASTRUCTURETUNNELING NANOTUBESHIGH-RESOLUTION MICROSCOPYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Giardia doudenalis (lamblia, intestinalis) is a protozoan parasite that inhabits the lumen of the upper small intestine of vertebrates, causing chronic abdominal pains and severe diarrhea, symptoms of giardiasis, a persistent and recurrent infection. This characteristic is mainly due to the presence of membrane variant-specific surface proteins (VSPs) that give this parasite the ability to successively infect the host through antigenic variation. Using high-resolution scanning microscopy (HR-SM), we observed the presence, formation, and extension of tunneling-nanotube-like surface structures in Giardia, especially following parasite challenges with VSP antibodies. They were seen all over the parasite surface, both in vitro and in vivo, showing that G. duodenalis nanotube formation occurs in complex environments such as the gut. In addition, we also observed that some of these nanotubes displayed a periodic strangulation that produces 100 nm vesicles that seemed to be released in a process similar to that previously observed in Trypanosoma brucei. The presence of nanotube-like structures in G. duodenalis highlights yet another strategy of cellular communication utilized by these parasites, whether between themselves or with the host cell.Fil: Midlej, Victor. Instituto Oswaldo Cruz ; Fundacion Oswaldo Cruz ; Ministerio de Salud de Brasil;Fil: Tenaglia, Albano Heraldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Luján, Hugo D.. Johannes Gutenberg Universitat Mainz; AlemaniaFil: de Souza, Wanderley. Universidade Federal Do Rio de Janeiro. Centro Nacional de Biologia Estrutural E Bioimagem.; BrasilMDPI2024-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/257090Midlej, Victor; Tenaglia, Albano Heraldo; Luján, Hugo D.; de Souza, Wanderley; Tunneling Nanotube-like Structures in Giardia duodenalis; MDPI; Cells; 13; 18; 9-2024; 1-132073-44092073-4409CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4409/13/18/1538info:eu-repo/semantics/altIdentifier/doi/10.3390/cells13181538info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:01:24Zoai:ri.conicet.gov.ar:11336/257090instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:01:24.669CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Tunneling Nanotube-like Structures in Giardia duodenalis
title Tunneling Nanotube-like Structures in Giardia duodenalis
spellingShingle Tunneling Nanotube-like Structures in Giardia duodenalis
Midlej, Victor
PROTOZOAN CELL
ULTRASTRUCTURE
TUNNELING NANOTUBES
HIGH-RESOLUTION MICROSCOPY
title_short Tunneling Nanotube-like Structures in Giardia duodenalis
title_full Tunneling Nanotube-like Structures in Giardia duodenalis
title_fullStr Tunneling Nanotube-like Structures in Giardia duodenalis
title_full_unstemmed Tunneling Nanotube-like Structures in Giardia duodenalis
title_sort Tunneling Nanotube-like Structures in Giardia duodenalis
dc.creator.none.fl_str_mv Midlej, Victor
Tenaglia, Albano Heraldo
Luján, Hugo D.
de Souza, Wanderley
author Midlej, Victor
author_facet Midlej, Victor
Tenaglia, Albano Heraldo
Luján, Hugo D.
de Souza, Wanderley
author_role author
author2 Tenaglia, Albano Heraldo
Luján, Hugo D.
de Souza, Wanderley
author2_role author
author
author
dc.subject.none.fl_str_mv PROTOZOAN CELL
ULTRASTRUCTURE
TUNNELING NANOTUBES
HIGH-RESOLUTION MICROSCOPY
topic PROTOZOAN CELL
ULTRASTRUCTURE
TUNNELING NANOTUBES
HIGH-RESOLUTION MICROSCOPY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Giardia doudenalis (lamblia, intestinalis) is a protozoan parasite that inhabits the lumen of the upper small intestine of vertebrates, causing chronic abdominal pains and severe diarrhea, symptoms of giardiasis, a persistent and recurrent infection. This characteristic is mainly due to the presence of membrane variant-specific surface proteins (VSPs) that give this parasite the ability to successively infect the host through antigenic variation. Using high-resolution scanning microscopy (HR-SM), we observed the presence, formation, and extension of tunneling-nanotube-like surface structures in Giardia, especially following parasite challenges with VSP antibodies. They were seen all over the parasite surface, both in vitro and in vivo, showing that G. duodenalis nanotube formation occurs in complex environments such as the gut. In addition, we also observed that some of these nanotubes displayed a periodic strangulation that produces 100 nm vesicles that seemed to be released in a process similar to that previously observed in Trypanosoma brucei. The presence of nanotube-like structures in G. duodenalis highlights yet another strategy of cellular communication utilized by these parasites, whether between themselves or with the host cell.
Fil: Midlej, Victor. Instituto Oswaldo Cruz ; Fundacion Oswaldo Cruz ; Ministerio de Salud de Brasil;
Fil: Tenaglia, Albano Heraldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Luján, Hugo D.. Johannes Gutenberg Universitat Mainz; Alemania
Fil: de Souza, Wanderley. Universidade Federal Do Rio de Janeiro. Centro Nacional de Biologia Estrutural E Bioimagem.; Brasil
description Giardia doudenalis (lamblia, intestinalis) is a protozoan parasite that inhabits the lumen of the upper small intestine of vertebrates, causing chronic abdominal pains and severe diarrhea, symptoms of giardiasis, a persistent and recurrent infection. This characteristic is mainly due to the presence of membrane variant-specific surface proteins (VSPs) that give this parasite the ability to successively infect the host through antigenic variation. Using high-resolution scanning microscopy (HR-SM), we observed the presence, formation, and extension of tunneling-nanotube-like surface structures in Giardia, especially following parasite challenges with VSP antibodies. They were seen all over the parasite surface, both in vitro and in vivo, showing that G. duodenalis nanotube formation occurs in complex environments such as the gut. In addition, we also observed that some of these nanotubes displayed a periodic strangulation that produces 100 nm vesicles that seemed to be released in a process similar to that previously observed in Trypanosoma brucei. The presence of nanotube-like structures in G. duodenalis highlights yet another strategy of cellular communication utilized by these parasites, whether between themselves or with the host cell.
publishDate 2024
dc.date.none.fl_str_mv 2024-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/257090
Midlej, Victor; Tenaglia, Albano Heraldo; Luján, Hugo D.; de Souza, Wanderley; Tunneling Nanotube-like Structures in Giardia duodenalis; MDPI; Cells; 13; 18; 9-2024; 1-13
2073-4409
2073-4409
CONICET Digital
CONICET
url http://hdl.handle.net/11336/257090
identifier_str_mv Midlej, Victor; Tenaglia, Albano Heraldo; Luján, Hugo D.; de Souza, Wanderley; Tunneling Nanotube-like Structures in Giardia duodenalis; MDPI; Cells; 13; 18; 9-2024; 1-13
2073-4409
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4409/13/18/1538
info:eu-repo/semantics/altIdentifier/doi/10.3390/cells13181538
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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