Transmission of Furcraea necrotic streak virus (FNSV) by Olpidium virulentus

Autores
Barrera, Gloria Patricia; Molano, Gina Marcela; Beltrán, Camilo; Alfonso Patarroyo, Manuel; Cerrudo, Carolina Susana; Belaich, Mariano Nicolas
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Furcraea necrotic streak virus (FNSV) is the causative agent of necrotic streak disease, also known as macana in fique crops (Furcraea spp.) resulting in damage to leaf fibres and economic losses. The rhizospheric fungus Olpidium spp. is present in the roots of affected plants and may play a role in the disease transmission. FNSV infection and the role of fungi were examined in macanavirus diseased plants in Colombia. Spherical, and icosahedral (24.7 ± 1.98 nm), non-enveloped virions with a granular surface were isolated from field fique plants showing signs of macana disease, and the viral genome was completely sequenced. Taxonomic status was assigned through sequence analysis (Macanavirus genus in Tombusviridae family). The variability of the virus pangenome was evaluated in diseased fique plants from ecologically different Andean regions that showed low gene flow. Olpidium virulentus zoospores, identified by internal transcribed spacer (ITS) sequencing and microscopic analysis, were associated with viral particles and resting spores in diseased fique roots. An in vitro virion?zoospore binding assay showed that FNSV and O. virulentus zoospores interacted. Transmission assays in lettuce (Lactuca sativa), a model plant used to study this virus, showed 100% infection when a preincubated mixture of zoospores and FNSV was added to roots, whereas only 33% infection occurred when FNSV was added alone. This demonstrated the ability of O. virulentus to act as a vector for FNSV, potentially enhancing viral transmissibility in field fique crops. This is the first report of FNSV being transmitted by O. virulentus, a rhizosphere fungus.
Fil: Barrera, Gloria Patricia. Centro de Investigación Tibaitatá; Colombia
Fil: Molano, Gina Marcela. Centro de Investigación Tibaitatá; Colombia
Fil: Beltrán, Camilo. Centro de Investigación Tibaitatá; Colombia
Fil: Alfonso Patarroyo, Manuel. Universidad Nacional de Colombia. Facultad de Medicina; Colombia
Fil: Cerrudo, Carolina Susana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; Argentina
Fil: Belaich, Mariano Nicolas. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Furcraea necrotic streak virus (FNSV)
Furcraea spp.
macana disease
Olpidium virulentus
Tombusviridae
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/237443
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/237443
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Transmission of Furcraea necrotic streak virus (FNSV) by Olpidium virulentusBarrera, Gloria PatriciaMolano, Gina MarcelaBeltrán, CamiloAlfonso Patarroyo, ManuelCerrudo, Carolina SusanaBelaich, Mariano NicolasFurcraea necrotic streak virus (FNSV)Furcraea spp.macana diseaseOlpidium virulentusTombusviridaehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Furcraea necrotic streak virus (FNSV) is the causative agent of necrotic streak disease, also known as macana in fique crops (Furcraea spp.) resulting in damage to leaf fibres and economic losses. The rhizospheric fungus Olpidium spp. is present in the roots of affected plants and may play a role in the disease transmission. FNSV infection and the role of fungi were examined in macanavirus diseased plants in Colombia. Spherical, and icosahedral (24.7 ± 1.98 nm), non-enveloped virions with a granular surface were isolated from field fique plants showing signs of macana disease, and the viral genome was completely sequenced. Taxonomic status was assigned through sequence analysis (Macanavirus genus in Tombusviridae family). The variability of the virus pangenome was evaluated in diseased fique plants from ecologically different Andean regions that showed low gene flow. Olpidium virulentus zoospores, identified by internal transcribed spacer (ITS) sequencing and microscopic analysis, were associated with viral particles and resting spores in diseased fique roots. An in vitro virion?zoospore binding assay showed that FNSV and O. virulentus zoospores interacted. Transmission assays in lettuce (Lactuca sativa), a model plant used to study this virus, showed 100% infection when a preincubated mixture of zoospores and FNSV was added to roots, whereas only 33% infection occurred when FNSV was added alone. This demonstrated the ability of O. virulentus to act as a vector for FNSV, potentially enhancing viral transmissibility in field fique crops. This is the first report of FNSV being transmitted by O. virulentus, a rhizosphere fungus.Fil: Barrera, Gloria Patricia. Centro de Investigación Tibaitatá; ColombiaFil: Molano, Gina Marcela. Centro de Investigación Tibaitatá; ColombiaFil: Beltrán, Camilo. Centro de Investigación Tibaitatá; ColombiaFil: Alfonso Patarroyo, Manuel. Universidad Nacional de Colombia. Facultad de Medicina; ColombiaFil: Cerrudo, Carolina Susana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; ArgentinaFil: Belaich, Mariano Nicolas. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley Blackwell Publishing, Inc2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/237443Barrera, Gloria Patricia; Molano, Gina Marcela; Beltrán, Camilo; Alfonso Patarroyo, Manuel; Cerrudo, Carolina Susana; et al.; Transmission of Furcraea necrotic streak virus (FNSV) by Olpidium virulentus; Wiley Blackwell Publishing, Inc; Plant Pathology; 72; 8; 5-2023; 1393-14050032-0862CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/ppa.13765info:eu-repo/semantics/altIdentifier/url/https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/ppa.13765info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:26:13Zoai:ri.conicet.gov.ar:11336/237443instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:26:13.685CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transmission of Furcraea necrotic streak virus (FNSV) by Olpidium virulentus
title Transmission of Furcraea necrotic streak virus (FNSV) by Olpidium virulentus
spellingShingle Transmission of Furcraea necrotic streak virus (FNSV) by Olpidium virulentus
Barrera, Gloria Patricia
Furcraea necrotic streak virus (FNSV)
Furcraea spp.
macana disease
Olpidium virulentus
Tombusviridae
title_short Transmission of Furcraea necrotic streak virus (FNSV) by Olpidium virulentus
title_full Transmission of Furcraea necrotic streak virus (FNSV) by Olpidium virulentus
title_fullStr Transmission of Furcraea necrotic streak virus (FNSV) by Olpidium virulentus
title_full_unstemmed Transmission of Furcraea necrotic streak virus (FNSV) by Olpidium virulentus
title_sort Transmission of Furcraea necrotic streak virus (FNSV) by Olpidium virulentus
dc.creator.none.fl_str_mv Barrera, Gloria Patricia
Molano, Gina Marcela
Beltrán, Camilo
Alfonso Patarroyo, Manuel
Cerrudo, Carolina Susana
Belaich, Mariano Nicolas
author Barrera, Gloria Patricia
author_facet Barrera, Gloria Patricia
Molano, Gina Marcela
Beltrán, Camilo
Alfonso Patarroyo, Manuel
Cerrudo, Carolina Susana
Belaich, Mariano Nicolas
author_role author
author2 Molano, Gina Marcela
Beltrán, Camilo
Alfonso Patarroyo, Manuel
Cerrudo, Carolina Susana
Belaich, Mariano Nicolas
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Furcraea necrotic streak virus (FNSV)
Furcraea spp.
macana disease
Olpidium virulentus
Tombusviridae
topic Furcraea necrotic streak virus (FNSV)
Furcraea spp.
macana disease
Olpidium virulentus
Tombusviridae
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Furcraea necrotic streak virus (FNSV) is the causative agent of necrotic streak disease, also known as macana in fique crops (Furcraea spp.) resulting in damage to leaf fibres and economic losses. The rhizospheric fungus Olpidium spp. is present in the roots of affected plants and may play a role in the disease transmission. FNSV infection and the role of fungi were examined in macanavirus diseased plants in Colombia. Spherical, and icosahedral (24.7 ± 1.98 nm), non-enveloped virions with a granular surface were isolated from field fique plants showing signs of macana disease, and the viral genome was completely sequenced. Taxonomic status was assigned through sequence analysis (Macanavirus genus in Tombusviridae family). The variability of the virus pangenome was evaluated in diseased fique plants from ecologically different Andean regions that showed low gene flow. Olpidium virulentus zoospores, identified by internal transcribed spacer (ITS) sequencing and microscopic analysis, were associated with viral particles and resting spores in diseased fique roots. An in vitro virion?zoospore binding assay showed that FNSV and O. virulentus zoospores interacted. Transmission assays in lettuce (Lactuca sativa), a model plant used to study this virus, showed 100% infection when a preincubated mixture of zoospores and FNSV was added to roots, whereas only 33% infection occurred when FNSV was added alone. This demonstrated the ability of O. virulentus to act as a vector for FNSV, potentially enhancing viral transmissibility in field fique crops. This is the first report of FNSV being transmitted by O. virulentus, a rhizosphere fungus.
Fil: Barrera, Gloria Patricia. Centro de Investigación Tibaitatá; Colombia
Fil: Molano, Gina Marcela. Centro de Investigación Tibaitatá; Colombia
Fil: Beltrán, Camilo. Centro de Investigación Tibaitatá; Colombia
Fil: Alfonso Patarroyo, Manuel. Universidad Nacional de Colombia. Facultad de Medicina; Colombia
Fil: Cerrudo, Carolina Susana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; Argentina
Fil: Belaich, Mariano Nicolas. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Furcraea necrotic streak virus (FNSV) is the causative agent of necrotic streak disease, also known as macana in fique crops (Furcraea spp.) resulting in damage to leaf fibres and economic losses. The rhizospheric fungus Olpidium spp. is present in the roots of affected plants and may play a role in the disease transmission. FNSV infection and the role of fungi were examined in macanavirus diseased plants in Colombia. Spherical, and icosahedral (24.7 ± 1.98 nm), non-enveloped virions with a granular surface were isolated from field fique plants showing signs of macana disease, and the viral genome was completely sequenced. Taxonomic status was assigned through sequence analysis (Macanavirus genus in Tombusviridae family). The variability of the virus pangenome was evaluated in diseased fique plants from ecologically different Andean regions that showed low gene flow. Olpidium virulentus zoospores, identified by internal transcribed spacer (ITS) sequencing and microscopic analysis, were associated with viral particles and resting spores in diseased fique roots. An in vitro virion?zoospore binding assay showed that FNSV and O. virulentus zoospores interacted. Transmission assays in lettuce (Lactuca sativa), a model plant used to study this virus, showed 100% infection when a preincubated mixture of zoospores and FNSV was added to roots, whereas only 33% infection occurred when FNSV was added alone. This demonstrated the ability of O. virulentus to act as a vector for FNSV, potentially enhancing viral transmissibility in field fique crops. This is the first report of FNSV being transmitted by O. virulentus, a rhizosphere fungus.
publishDate 2023
dc.date.none.fl_str_mv 2023-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/237443
Barrera, Gloria Patricia; Molano, Gina Marcela; Beltrán, Camilo; Alfonso Patarroyo, Manuel; Cerrudo, Carolina Susana; et al.; Transmission of Furcraea necrotic streak virus (FNSV) by Olpidium virulentus; Wiley Blackwell Publishing, Inc; Plant Pathology; 72; 8; 5-2023; 1393-1405
0032-0862
CONICET Digital
CONICET
url http://hdl.handle.net/11336/237443
identifier_str_mv Barrera, Gloria Patricia; Molano, Gina Marcela; Beltrán, Camilo; Alfonso Patarroyo, Manuel; Cerrudo, Carolina Susana; et al.; Transmission of Furcraea necrotic streak virus (FNSV) by Olpidium virulentus; Wiley Blackwell Publishing, Inc; Plant Pathology; 72; 8; 5-2023; 1393-1405
0032-0862
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/ppa.13765
info:eu-repo/semantics/altIdentifier/url/https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/ppa.13765
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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