ATP and adenosine modulate acetylcholine release through P2Y and P1 receptors at The efferent-inner hair cell synapse in the developing inner ear

Autores
Alvarez Heduan, Facundo; Katz, Eleonora; Goutman, Juan Diego; Elgoyhen, Ana Belen
Año de publicación
2014
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Before the onset of hearing (postnatal day 12 in mice) inner hair cells (IHCs) are transiently innervated by medial olivocochlear (MOC) efferent fibers. Acetylcholine (ACh) released by these fibers activates alpha9alpha10 nicotinic receptors coupled to SK2 calcium-activated potassium channels, leading to inhibitory post synaptic currents (IPSCs). During this period, IHCs fire spontaneous sensory-independent action potentials that are required for normal development of the auditory pathway. This activity is driven and/or modulated by ATP released from cochlear supporting cells. ACh release from efferent fibers also contributes to this modulation. By electrically stimulating MOC fibers and recording IPSCs, we showed that ATP decreases ACh release in a reversible and concentration-dependent manner. In this work, we demonstrate that this effect is through P2Y receptor activation, as the specific P2Y agonist 2-MeSADP mimicked the effect of ATP. Moreover, the non-hydrolyzable ATP analog ATPgammaS decreased ACh release as well, indicating that this modulation is driven by ATP itself. We further tested if adenosine can also modulate ACh release. Adenosine reversibly decreased the quantal content. CGS15943, a specific P1 receptor antagonist, abolished the effect of adenosine. On the other hand NECA, a specific P1 agonist, decreased ACh release. Our results suggest that both ATP and adenosine inhibit ACh release at the MOC-IHC synapse through the activation of P2Y and P1 receptors, respectively.
Fil: Alvarez Heduan, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Katz, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Goutman, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
XXIX Annual Meeting and SAN-ISN small conference and course
Huerta Grande
Argentina
Sociedad Argentina de Investigación en Neurosciencias
Materia
ATP
IHC
MOC
ACH
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/215624
Acceder
id CONICETDig_71b9733d380a72eba8e5bd0c39d953e6
oai_identifier_str oai:ri.conicet.gov.ar:11336/215624
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling ATP and adenosine modulate acetylcholine release through P2Y and P1 receptors at The efferent-inner hair cell synapse in the developing inner earAlvarez Heduan, FacundoKatz, EleonoraGoutman, Juan DiegoElgoyhen, Ana BelenATPIHCMOCACHhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Before the onset of hearing (postnatal day 12 in mice) inner hair cells (IHCs) are transiently innervated by medial olivocochlear (MOC) efferent fibers. Acetylcholine (ACh) released by these fibers activates alpha9alpha10 nicotinic receptors coupled to SK2 calcium-activated potassium channels, leading to inhibitory post synaptic currents (IPSCs). During this period, IHCs fire spontaneous sensory-independent action potentials that are required for normal development of the auditory pathway. This activity is driven and/or modulated by ATP released from cochlear supporting cells. ACh release from efferent fibers also contributes to this modulation. By electrically stimulating MOC fibers and recording IPSCs, we showed that ATP decreases ACh release in a reversible and concentration-dependent manner. In this work, we demonstrate that this effect is through P2Y receptor activation, as the specific P2Y agonist 2-MeSADP mimicked the effect of ATP. Moreover, the non-hydrolyzable ATP analog ATPgammaS decreased ACh release as well, indicating that this modulation is driven by ATP itself. We further tested if adenosine can also modulate ACh release. Adenosine reversibly decreased the quantal content. CGS15943, a specific P1 receptor antagonist, abolished the effect of adenosine. On the other hand NECA, a specific P1 agonist, decreased ACh release. Our results suggest that both ATP and adenosine inhibit ACh release at the MOC-IHC synapse through the activation of P2Y and P1 receptors, respectively.Fil: Alvarez Heduan, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Katz, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Goutman, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaXXIX Annual Meeting and SAN-ISN small conference and courseHuerta GrandeArgentinaSociedad Argentina de Investigación en NeuroscienciasSociedad Argentina de Investigación en Neurociencias2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/215624ATP and adenosine modulate acetylcholine release through P2Y and P1 receptors at The efferent-inner hair cell synapse in the developing inner ear; XXIX Annual Meeting and SAN-ISN small conference and course; Huerta Grande; Argentina; 2014; 1-1CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/wp-content/uploads/2022/01/SAN2014_Huerta-Grande_libro-de-resumenes.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:55:28Zoai:ri.conicet.gov.ar:11336/215624instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:55:28.814CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv ATP and adenosine modulate acetylcholine release through P2Y and P1 receptors at The efferent-inner hair cell synapse in the developing inner ear
title ATP and adenosine modulate acetylcholine release through P2Y and P1 receptors at The efferent-inner hair cell synapse in the developing inner ear
spellingShingle ATP and adenosine modulate acetylcholine release through P2Y and P1 receptors at The efferent-inner hair cell synapse in the developing inner ear
Alvarez Heduan, Facundo
ATP
IHC
MOC
ACH
title_short ATP and adenosine modulate acetylcholine release through P2Y and P1 receptors at The efferent-inner hair cell synapse in the developing inner ear
title_full ATP and adenosine modulate acetylcholine release through P2Y and P1 receptors at The efferent-inner hair cell synapse in the developing inner ear
title_fullStr ATP and adenosine modulate acetylcholine release through P2Y and P1 receptors at The efferent-inner hair cell synapse in the developing inner ear
title_full_unstemmed ATP and adenosine modulate acetylcholine release through P2Y and P1 receptors at The efferent-inner hair cell synapse in the developing inner ear
title_sort ATP and adenosine modulate acetylcholine release through P2Y and P1 receptors at The efferent-inner hair cell synapse in the developing inner ear
dc.creator.none.fl_str_mv Alvarez Heduan, Facundo
Katz, Eleonora
Goutman, Juan Diego
Elgoyhen, Ana Belen
author Alvarez Heduan, Facundo
author_facet Alvarez Heduan, Facundo
Katz, Eleonora
Goutman, Juan Diego
Elgoyhen, Ana Belen
author_role author
author2 Katz, Eleonora
Goutman, Juan Diego
Elgoyhen, Ana Belen
author2_role author
author
author
dc.subject.none.fl_str_mv ATP
IHC
MOC
ACH
topic ATP
IHC
MOC
ACH
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Before the onset of hearing (postnatal day 12 in mice) inner hair cells (IHCs) are transiently innervated by medial olivocochlear (MOC) efferent fibers. Acetylcholine (ACh) released by these fibers activates alpha9alpha10 nicotinic receptors coupled to SK2 calcium-activated potassium channels, leading to inhibitory post synaptic currents (IPSCs). During this period, IHCs fire spontaneous sensory-independent action potentials that are required for normal development of the auditory pathway. This activity is driven and/or modulated by ATP released from cochlear supporting cells. ACh release from efferent fibers also contributes to this modulation. By electrically stimulating MOC fibers and recording IPSCs, we showed that ATP decreases ACh release in a reversible and concentration-dependent manner. In this work, we demonstrate that this effect is through P2Y receptor activation, as the specific P2Y agonist 2-MeSADP mimicked the effect of ATP. Moreover, the non-hydrolyzable ATP analog ATPgammaS decreased ACh release as well, indicating that this modulation is driven by ATP itself. We further tested if adenosine can also modulate ACh release. Adenosine reversibly decreased the quantal content. CGS15943, a specific P1 receptor antagonist, abolished the effect of adenosine. On the other hand NECA, a specific P1 agonist, decreased ACh release. Our results suggest that both ATP and adenosine inhibit ACh release at the MOC-IHC synapse through the activation of P2Y and P1 receptors, respectively.
Fil: Alvarez Heduan, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Katz, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Goutman, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
XXIX Annual Meeting and SAN-ISN small conference and course
Huerta Grande
Argentina
Sociedad Argentina de Investigación en Neurosciencias
description Before the onset of hearing (postnatal day 12 in mice) inner hair cells (IHCs) are transiently innervated by medial olivocochlear (MOC) efferent fibers. Acetylcholine (ACh) released by these fibers activates alpha9alpha10 nicotinic receptors coupled to SK2 calcium-activated potassium channels, leading to inhibitory post synaptic currents (IPSCs). During this period, IHCs fire spontaneous sensory-independent action potentials that are required for normal development of the auditory pathway. This activity is driven and/or modulated by ATP released from cochlear supporting cells. ACh release from efferent fibers also contributes to this modulation. By electrically stimulating MOC fibers and recording IPSCs, we showed that ATP decreases ACh release in a reversible and concentration-dependent manner. In this work, we demonstrate that this effect is through P2Y receptor activation, as the specific P2Y agonist 2-MeSADP mimicked the effect of ATP. Moreover, the non-hydrolyzable ATP analog ATPgammaS decreased ACh release as well, indicating that this modulation is driven by ATP itself. We further tested if adenosine can also modulate ACh release. Adenosine reversibly decreased the quantal content. CGS15943, a specific P1 receptor antagonist, abolished the effect of adenosine. On the other hand NECA, a specific P1 agonist, decreased ACh release. Our results suggest that both ATP and adenosine inhibit ACh release at the MOC-IHC synapse through the activation of P2Y and P1 receptors, respectively.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/215624
ATP and adenosine modulate acetylcholine release through P2Y and P1 receptors at The efferent-inner hair cell synapse in the developing inner ear; XXIX Annual Meeting and SAN-ISN small conference and course; Huerta Grande; Argentina; 2014; 1-1
CONICET Digital
CONICET
url http://hdl.handle.net/11336/215624
identifier_str_mv ATP and adenosine modulate acetylcholine release through P2Y and P1 receptors at The efferent-inner hair cell synapse in the developing inner ear; XXIX Annual Meeting and SAN-ISN small conference and course; Huerta Grande; Argentina; 2014; 1-1
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/wp-content/uploads/2022/01/SAN2014_Huerta-Grande_libro-de-resumenes.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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