From the isolation of bacteriocinogenic LAB strains to the application for fish paste biopreservation

Autores
Delcarlo, Sofía Belén; Parada, Romina Belén; Schelegueda, Laura Inés; Vallejo, Marisol; Marguet, Emilio Rogelio; Campos, Carmen Adriana
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aims were to isolate, characterize and quantifylactic acid bacteria?s (LAB) antimicrobial activity, and evaluate their application on fish paste. One hundred and thirty two LAB were isolated from mussels of the Argentine coast. From all, 22 LAB isolated which?s cell-free supernatant (CFS) presented activity against Listeria innocua and Lactobacillus plantarum were identified as Enterococcus mundtii by biochemical tests and 16S rDNA gene phylogenetic analyses. None of selected strains presented virulence factors. All of them presented the gene that codifies either for mundticin KS, or for mundticin KS and enterocin L50A. Their CFS showed activity against Gram positive bacteria. The interaction among binary mixtures of CFS was assessed against L. innocua, and an additive response was observed. The strain E. mundtii STw38 was selected because of its high capacity to grow and produce bacteriocins at refrigeration temperatures. It was applied as a protective culture to fish paste stored at 4°C. In a first stage fish paste-systems were air-packed, and then, vacuum-packed. In both cases, E. mundtii STw38 survived storage conditions and succeeded in reducing the development of native flora of fish paste. Results suggest that E. mundtii STw38 is a promising strain to be used for fish biopreservation.
Fil: Delcarlo, Sofía Belén. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Químicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Químicos; Argentina
Fil: Parada, Romina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina
Fil: Schelegueda, Laura Inés. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Químicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Químicos; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina
Fil: Vallejo, Marisol. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marguet, Emilio Rogelio. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Campos, Carmen Adriana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Químicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Químicos; Argentina
Materia
BACTERIOCIN
ENTEROCOCCUS MUNDTII
FISH BIOPRESERVATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/119067
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling From the isolation of bacteriocinogenic LAB strains to the application for fish paste biopreservationDelcarlo, Sofía BelénParada, Romina BelénSchelegueda, Laura InésVallejo, MarisolMarguet, Emilio RogelioCampos, Carmen AdrianaBACTERIOCINENTEROCOCCUS MUNDTIIFISH BIOPRESERVATIONhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2The aims were to isolate, characterize and quantifylactic acid bacteria?s (LAB) antimicrobial activity, and evaluate their application on fish paste. One hundred and thirty two LAB were isolated from mussels of the Argentine coast. From all, 22 LAB isolated which?s cell-free supernatant (CFS) presented activity against Listeria innocua and Lactobacillus plantarum were identified as Enterococcus mundtii by biochemical tests and 16S rDNA gene phylogenetic analyses. None of selected strains presented virulence factors. All of them presented the gene that codifies either for mundticin KS, or for mundticin KS and enterocin L50A. Their CFS showed activity against Gram positive bacteria. The interaction among binary mixtures of CFS was assessed against L. innocua, and an additive response was observed. The strain E. mundtii STw38 was selected because of its high capacity to grow and produce bacteriocins at refrigeration temperatures. It was applied as a protective culture to fish paste stored at 4°C. In a first stage fish paste-systems were air-packed, and then, vacuum-packed. In both cases, E. mundtii STw38 survived storage conditions and succeeded in reducing the development of native flora of fish paste. Results suggest that E. mundtii STw38 is a promising strain to be used for fish biopreservation.Fil: Delcarlo, Sofía Belén. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Químicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Químicos; ArgentinaFil: Parada, Romina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco"; ArgentinaFil: Schelegueda, Laura Inés. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Químicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Químicos; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; ArgentinaFil: Vallejo, Marisol. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marguet, Emilio Rogelio. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Campos, Carmen Adriana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Químicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Químicos; ArgentinaElsevier Science2019-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/119067Delcarlo, Sofía Belén; Parada, Romina Belén; Schelegueda, Laura Inés; Vallejo, Marisol; Marguet, Emilio Rogelio; et al.; From the isolation of bacteriocinogenic LAB strains to the application for fish paste biopreservation; Elsevier Science; LWT - Food Science and Technology; 110; 8-2019; 239-2460023-6438CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0023643819303913info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2019.04.079info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:18Zoai:ri.conicet.gov.ar:11336/119067instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:19.12CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv From the isolation of bacteriocinogenic LAB strains to the application for fish paste biopreservation
title From the isolation of bacteriocinogenic LAB strains to the application for fish paste biopreservation
spellingShingle From the isolation of bacteriocinogenic LAB strains to the application for fish paste biopreservation
Delcarlo, Sofía Belén
BACTERIOCIN
ENTEROCOCCUS MUNDTII
FISH BIOPRESERVATION
title_short From the isolation of bacteriocinogenic LAB strains to the application for fish paste biopreservation
title_full From the isolation of bacteriocinogenic LAB strains to the application for fish paste biopreservation
title_fullStr From the isolation of bacteriocinogenic LAB strains to the application for fish paste biopreservation
title_full_unstemmed From the isolation of bacteriocinogenic LAB strains to the application for fish paste biopreservation
title_sort From the isolation of bacteriocinogenic LAB strains to the application for fish paste biopreservation
dc.creator.none.fl_str_mv Delcarlo, Sofía Belén
Parada, Romina Belén
Schelegueda, Laura Inés
Vallejo, Marisol
Marguet, Emilio Rogelio
Campos, Carmen Adriana
author Delcarlo, Sofía Belén
author_facet Delcarlo, Sofía Belén
Parada, Romina Belén
Schelegueda, Laura Inés
Vallejo, Marisol
Marguet, Emilio Rogelio
Campos, Carmen Adriana
author_role author
author2 Parada, Romina Belén
Schelegueda, Laura Inés
Vallejo, Marisol
Marguet, Emilio Rogelio
Campos, Carmen Adriana
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BACTERIOCIN
ENTEROCOCCUS MUNDTII
FISH BIOPRESERVATION
topic BACTERIOCIN
ENTEROCOCCUS MUNDTII
FISH BIOPRESERVATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The aims were to isolate, characterize and quantifylactic acid bacteria?s (LAB) antimicrobial activity, and evaluate their application on fish paste. One hundred and thirty two LAB were isolated from mussels of the Argentine coast. From all, 22 LAB isolated which?s cell-free supernatant (CFS) presented activity against Listeria innocua and Lactobacillus plantarum were identified as Enterococcus mundtii by biochemical tests and 16S rDNA gene phylogenetic analyses. None of selected strains presented virulence factors. All of them presented the gene that codifies either for mundticin KS, or for mundticin KS and enterocin L50A. Their CFS showed activity against Gram positive bacteria. The interaction among binary mixtures of CFS was assessed against L. innocua, and an additive response was observed. The strain E. mundtii STw38 was selected because of its high capacity to grow and produce bacteriocins at refrigeration temperatures. It was applied as a protective culture to fish paste stored at 4°C. In a first stage fish paste-systems were air-packed, and then, vacuum-packed. In both cases, E. mundtii STw38 survived storage conditions and succeeded in reducing the development of native flora of fish paste. Results suggest that E. mundtii STw38 is a promising strain to be used for fish biopreservation.
Fil: Delcarlo, Sofía Belén. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Químicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Químicos; Argentina
Fil: Parada, Romina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina
Fil: Schelegueda, Laura Inés. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Químicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Químicos; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina
Fil: Vallejo, Marisol. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marguet, Emilio Rogelio. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Campos, Carmen Adriana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Químicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Químicos; Argentina
description The aims were to isolate, characterize and quantifylactic acid bacteria?s (LAB) antimicrobial activity, and evaluate their application on fish paste. One hundred and thirty two LAB were isolated from mussels of the Argentine coast. From all, 22 LAB isolated which?s cell-free supernatant (CFS) presented activity against Listeria innocua and Lactobacillus plantarum were identified as Enterococcus mundtii by biochemical tests and 16S rDNA gene phylogenetic analyses. None of selected strains presented virulence factors. All of them presented the gene that codifies either for mundticin KS, or for mundticin KS and enterocin L50A. Their CFS showed activity against Gram positive bacteria. The interaction among binary mixtures of CFS was assessed against L. innocua, and an additive response was observed. The strain E. mundtii STw38 was selected because of its high capacity to grow and produce bacteriocins at refrigeration temperatures. It was applied as a protective culture to fish paste stored at 4°C. In a first stage fish paste-systems were air-packed, and then, vacuum-packed. In both cases, E. mundtii STw38 survived storage conditions and succeeded in reducing the development of native flora of fish paste. Results suggest that E. mundtii STw38 is a promising strain to be used for fish biopreservation.
publishDate 2019
dc.date.none.fl_str_mv 2019-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/119067
Delcarlo, Sofía Belén; Parada, Romina Belén; Schelegueda, Laura Inés; Vallejo, Marisol; Marguet, Emilio Rogelio; et al.; From the isolation of bacteriocinogenic LAB strains to the application for fish paste biopreservation; Elsevier Science; LWT - Food Science and Technology; 110; 8-2019; 239-246
0023-6438
CONICET Digital
CONICET
url http://hdl.handle.net/11336/119067
identifier_str_mv Delcarlo, Sofía Belén; Parada, Romina Belén; Schelegueda, Laura Inés; Vallejo, Marisol; Marguet, Emilio Rogelio; et al.; From the isolation of bacteriocinogenic LAB strains to the application for fish paste biopreservation; Elsevier Science; LWT - Food Science and Technology; 110; 8-2019; 239-246
0023-6438
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0023643819303913
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2019.04.079
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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