Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis

Autores
Schelegueda, Laura Inés; Vallejo, Marisol; Gliemmo, María Fernanda; Marguet, Emilio Rogelio; Campos, Carmen Adriana
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aims of this study were to characterize an enterococci bacteriocinogenic strain and to evaluate its antimicrobial activity regarding its potential use for fish preservation. The bacterium, isolated from Odontesthes platensis in the Patagonian region of Argentine was identified as Enterococcus mundtii by means of biochemical test and 16S rDNA gene phylogenetic analyses. Furthermore, the gene that codifies mundticin KS was detected by PCR techniques. The bacteriocin was able to inhibit Gram positive and negative bacteria. In particular at pH 5.50, it was able to control the growth of fish bacterial flora. Construction of isobolograms and value of fractional inhibitory concentrations index showed that its use together with chitosan and sodium lactate (SL) exerted a synergic action on the inhibition of Listeria innocua, Shewanella putrefaciens and the psychrophilic flora isolated from fish. The use of the mentioned mixture of antimicrobials promoted strong changes on L. innocua and S. putrefaciens cellular components as shown by differential scanning calorimetry. Results suggest that the combination of the bacteriocin with chitosan and SL would be useful to reduce the amount of antimicrobials added to foods and to prevent the growth of spoilage bacteria and pathogenic microorganisms, such as Listeria monocytogenes.
Fil: Schelegueda, Laura Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina
Fil: Vallejo, Marisol. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia; Argentina
Fil: Gliemmo, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina
Fil: Marguet, Emilio Rogelio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia; Argentina
Fil: Campos, Carmen Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina
Materia
Bacteriocin
Enterococcus Mundtii
Fish Preservation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/45482
Acceder
id CONICETDig_0b7acaad59796d7cc872605b4b50f056
oai_identifier_str oai:ri.conicet.gov.ar:11336/45482
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensisSchelegueda, Laura InésVallejo, MarisolGliemmo, María FernandaMarguet, Emilio RogelioCampos, Carmen AdrianaBacteriocinEnterococcus MundtiiFish Preservationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aims of this study were to characterize an enterococci bacteriocinogenic strain and to evaluate its antimicrobial activity regarding its potential use for fish preservation. The bacterium, isolated from Odontesthes platensis in the Patagonian region of Argentine was identified as Enterococcus mundtii by means of biochemical test and 16S rDNA gene phylogenetic analyses. Furthermore, the gene that codifies mundticin KS was detected by PCR techniques. The bacteriocin was able to inhibit Gram positive and negative bacteria. In particular at pH 5.50, it was able to control the growth of fish bacterial flora. Construction of isobolograms and value of fractional inhibitory concentrations index showed that its use together with chitosan and sodium lactate (SL) exerted a synergic action on the inhibition of Listeria innocua, Shewanella putrefaciens and the psychrophilic flora isolated from fish. The use of the mentioned mixture of antimicrobials promoted strong changes on L. innocua and S. putrefaciens cellular components as shown by differential scanning calorimetry. Results suggest that the combination of the bacteriocin with chitosan and SL would be useful to reduce the amount of antimicrobials added to foods and to prevent the growth of spoilage bacteria and pathogenic microorganisms, such as Listeria monocytogenes.Fil: Schelegueda, Laura Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; ArgentinaFil: Vallejo, Marisol. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia; ArgentinaFil: Gliemmo, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; ArgentinaFil: Marguet, Emilio Rogelio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia; ArgentinaFil: Campos, Carmen Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; ArgentinaElsevier Science2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/45482Schelegueda, Laura Inés; Vallejo, Marisol; Gliemmo, María Fernanda; Marguet, Emilio Rogelio; Campos, Carmen Adriana; Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis; Elsevier Science; LWT - Food Science and Technology; 64; 2; 12-2015; 794-8010023-6438CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2015.06.017info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0023643815004442info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:34Zoai:ri.conicet.gov.ar:11336/45482instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:34.788CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis
title Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis
spellingShingle Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis
Schelegueda, Laura Inés
Bacteriocin
Enterococcus Mundtii
Fish Preservation
title_short Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis
title_full Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis
title_fullStr Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis
title_full_unstemmed Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis
title_sort Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis
dc.creator.none.fl_str_mv Schelegueda, Laura Inés
Vallejo, Marisol
Gliemmo, María Fernanda
Marguet, Emilio Rogelio
Campos, Carmen Adriana
author Schelegueda, Laura Inés
author_facet Schelegueda, Laura Inés
Vallejo, Marisol
Gliemmo, María Fernanda
Marguet, Emilio Rogelio
Campos, Carmen Adriana
author_role author
author2 Vallejo, Marisol
Gliemmo, María Fernanda
Marguet, Emilio Rogelio
Campos, Carmen Adriana
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bacteriocin
Enterococcus Mundtii
Fish Preservation
topic Bacteriocin
Enterococcus Mundtii
Fish Preservation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aims of this study were to characterize an enterococci bacteriocinogenic strain and to evaluate its antimicrobial activity regarding its potential use for fish preservation. The bacterium, isolated from Odontesthes platensis in the Patagonian region of Argentine was identified as Enterococcus mundtii by means of biochemical test and 16S rDNA gene phylogenetic analyses. Furthermore, the gene that codifies mundticin KS was detected by PCR techniques. The bacteriocin was able to inhibit Gram positive and negative bacteria. In particular at pH 5.50, it was able to control the growth of fish bacterial flora. Construction of isobolograms and value of fractional inhibitory concentrations index showed that its use together with chitosan and sodium lactate (SL) exerted a synergic action on the inhibition of Listeria innocua, Shewanella putrefaciens and the psychrophilic flora isolated from fish. The use of the mentioned mixture of antimicrobials promoted strong changes on L. innocua and S. putrefaciens cellular components as shown by differential scanning calorimetry. Results suggest that the combination of the bacteriocin with chitosan and SL would be useful to reduce the amount of antimicrobials added to foods and to prevent the growth of spoilage bacteria and pathogenic microorganisms, such as Listeria monocytogenes.
Fil: Schelegueda, Laura Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina
Fil: Vallejo, Marisol. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia; Argentina
Fil: Gliemmo, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina
Fil: Marguet, Emilio Rogelio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia; Argentina
Fil: Campos, Carmen Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina
description The aims of this study were to characterize an enterococci bacteriocinogenic strain and to evaluate its antimicrobial activity regarding its potential use for fish preservation. The bacterium, isolated from Odontesthes platensis in the Patagonian region of Argentine was identified as Enterococcus mundtii by means of biochemical test and 16S rDNA gene phylogenetic analyses. Furthermore, the gene that codifies mundticin KS was detected by PCR techniques. The bacteriocin was able to inhibit Gram positive and negative bacteria. In particular at pH 5.50, it was able to control the growth of fish bacterial flora. Construction of isobolograms and value of fractional inhibitory concentrations index showed that its use together with chitosan and sodium lactate (SL) exerted a synergic action on the inhibition of Listeria innocua, Shewanella putrefaciens and the psychrophilic flora isolated from fish. The use of the mentioned mixture of antimicrobials promoted strong changes on L. innocua and S. putrefaciens cellular components as shown by differential scanning calorimetry. Results suggest that the combination of the bacteriocin with chitosan and SL would be useful to reduce the amount of antimicrobials added to foods and to prevent the growth of spoilage bacteria and pathogenic microorganisms, such as Listeria monocytogenes.
publishDate 2015
dc.date.none.fl_str_mv 2015-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/45482
Schelegueda, Laura Inés; Vallejo, Marisol; Gliemmo, María Fernanda; Marguet, Emilio Rogelio; Campos, Carmen Adriana; Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis; Elsevier Science; LWT - Food Science and Technology; 64; 2; 12-2015; 794-801
0023-6438
CONICET Digital
CONICET
url http://hdl.handle.net/11336/45482
identifier_str_mv Schelegueda, Laura Inés; Vallejo, Marisol; Gliemmo, María Fernanda; Marguet, Emilio Rogelio; Campos, Carmen Adriana; Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis; Elsevier Science; LWT - Food Science and Technology; 64; 2; 12-2015; 794-801
0023-6438
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2015.06.017
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0023643815004442
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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