Brucella abortus RNA diminishes IFN-gamma-induced MHC-I molecules in different line regular and tumor cells

Autores
Serafino, Agustina; Bertinat, Yasmín Ayelén; Bueno, Jorgelina; Milillo, María Ayelén; Barrionuevo, Paula
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Brucella abortus (Ba) is an intracellular pathogen capable of surviving inside macrophages. Since the disease is presented in multiple forms, many different cells are susceptible to be infected by Ba. We have previously demonstrated that Ba RNA diminishes the IFN-ɤ-induced MHC-I surface expression in human macrophages, retaining them within the Golgi Apparatus (GA). However, we acknowledged whether this event could be triggered in other cells able to be infected with Ba. So, we stimulated the lung epithelium cell line (Calu-6) and the endothelial microvasculature cell line (HMEC) with different doses of Ba RNA in the presence of IFN-ɤ. MHC-I expression was assessed by flow cytometry. Ba RNA (10 ug/ml) diminished the IFN-ɤ-induced MHC-I surface expression (p<0.05) in both cell lines. Next, by confocal microscopy, we observed colocalization of MHC-I and GA marker GM130 in CALU-6 cells treated for 48h, although to a lesser extent than in human macrophages (p<0.05). Conversely to what we expected, supernatants from Calu-6-Ba RNA-treated cells had higher IL-8 production compared to those from untreated cells (p<0.05). In addition, a decrease in MHC-I on the surface could be accompanied by an activation of Natural Killer (NK) cells. These cells are key for the defense against multiple tumors. Therefore, a question of great importance is: what is the relevance of MHC-I modulation in the context of other pathologies in which NK response is critical? For this we started by stimulating the human glioblastoma cells (U251) with different doses of Ba RNA in the presence of IFN-ɤ. MHC-I expression was assessed by flow cytometry. Ba RNA diminished the IFN-ɤ-induced MHC-I surface expression (p<0.05) in U251. Together these results show that Ba could persist successfully within the host, remaining unnoticed and evading CD8+ T cell surveillance. On the other hand, the decrease in MHC-I could enhance the subsequent cytotoxic response against multiple tumors.
Fil: Serafino, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Bertinat, Yasmín Ayelén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Bueno, Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Milillo, María Ayelén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Barrionuevo, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Reunión Anual de Sociedades de Biociencias 2022; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad argentina de inmunología; Reunión anual 2022 de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Materia
MODULATION OF MHC-I
RNA
BRUCELLA ABORTUS
GOLGI APPARATUS
CALU-6
U251
TUMORS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/237490
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Brucella abortus RNA diminishes IFN-gamma-induced MHC-I molecules in different line regular and tumor cellsSerafino, AgustinaBertinat, Yasmín AyelénBueno, JorgelinaMilillo, María AyelénBarrionuevo, PaulaMODULATION OF MHC-IRNABRUCELLA ABORTUSGOLGI APPARATUSCALU-6U251TUMORShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Brucella abortus (Ba) is an intracellular pathogen capable of surviving inside macrophages. Since the disease is presented in multiple forms, many different cells are susceptible to be infected by Ba. We have previously demonstrated that Ba RNA diminishes the IFN-ɤ-induced MHC-I surface expression in human macrophages, retaining them within the Golgi Apparatus (GA). However, we acknowledged whether this event could be triggered in other cells able to be infected with Ba. So, we stimulated the lung epithelium cell line (Calu-6) and the endothelial microvasculature cell line (HMEC) with different doses of Ba RNA in the presence of IFN-ɤ. MHC-I expression was assessed by flow cytometry. Ba RNA (10 ug/ml) diminished the IFN-ɤ-induced MHC-I surface expression (p<0.05) in both cell lines. Next, by confocal microscopy, we observed colocalization of MHC-I and GA marker GM130 in CALU-6 cells treated for 48h, although to a lesser extent than in human macrophages (p<0.05). Conversely to what we expected, supernatants from Calu-6-Ba RNA-treated cells had higher IL-8 production compared to those from untreated cells (p<0.05). In addition, a decrease in MHC-I on the surface could be accompanied by an activation of Natural Killer (NK) cells. These cells are key for the defense against multiple tumors. Therefore, a question of great importance is: what is the relevance of MHC-I modulation in the context of other pathologies in which NK response is critical? For this we started by stimulating the human glioblastoma cells (U251) with different doses of Ba RNA in the presence of IFN-ɤ. MHC-I expression was assessed by flow cytometry. Ba RNA diminished the IFN-ɤ-induced MHC-I surface expression (p<0.05) in U251. Together these results show that Ba could persist successfully within the host, remaining unnoticed and evading CD8+ T cell surveillance. On the other hand, the decrease in MHC-I could enhance the subsequent cytotoxic response against multiple tumors.Fil: Serafino, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Bertinat, Yasmín Ayelén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Bueno, Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Milillo, María Ayelén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Barrionuevo, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaReunión Anual de Sociedades de Biociencias 2022; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad argentina de inmunología; Reunión anual 2022 de la Sociedad Argentina de FisiologíaMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaFundación Revista Medicina2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/237490Brucella abortus RNA diminishes IFN-gamma-induced MHC-I molecules in different line regular and tumor cells; Reunión Anual de Sociedades de Biociencias 2022; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad argentina de inmunología; Reunión anual 2022 de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2022; 150-1501669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol82-22/s5/1s5.pdfinfo:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2020/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:37:27Zoai:ri.conicet.gov.ar:11336/237490instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:37:27.748CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Brucella abortus RNA diminishes IFN-gamma-induced MHC-I molecules in different line regular and tumor cells
title Brucella abortus RNA diminishes IFN-gamma-induced MHC-I molecules in different line regular and tumor cells
spellingShingle Brucella abortus RNA diminishes IFN-gamma-induced MHC-I molecules in different line regular and tumor cells
Serafino, Agustina
MODULATION OF MHC-I
RNA
BRUCELLA ABORTUS
GOLGI APPARATUS
CALU-6
U251
TUMORS
title_short Brucella abortus RNA diminishes IFN-gamma-induced MHC-I molecules in different line regular and tumor cells
title_full Brucella abortus RNA diminishes IFN-gamma-induced MHC-I molecules in different line regular and tumor cells
title_fullStr Brucella abortus RNA diminishes IFN-gamma-induced MHC-I molecules in different line regular and tumor cells
title_full_unstemmed Brucella abortus RNA diminishes IFN-gamma-induced MHC-I molecules in different line regular and tumor cells
title_sort Brucella abortus RNA diminishes IFN-gamma-induced MHC-I molecules in different line regular and tumor cells
dc.creator.none.fl_str_mv Serafino, Agustina
Bertinat, Yasmín Ayelén
Bueno, Jorgelina
Milillo, María Ayelén
Barrionuevo, Paula
author Serafino, Agustina
author_facet Serafino, Agustina
Bertinat, Yasmín Ayelén
Bueno, Jorgelina
Milillo, María Ayelén
Barrionuevo, Paula
author_role author
author2 Bertinat, Yasmín Ayelén
Bueno, Jorgelina
Milillo, María Ayelén
Barrionuevo, Paula
author2_role author
author
author
author
dc.subject.none.fl_str_mv MODULATION OF MHC-I
RNA
BRUCELLA ABORTUS
GOLGI APPARATUS
CALU-6
U251
TUMORS
topic MODULATION OF MHC-I
RNA
BRUCELLA ABORTUS
GOLGI APPARATUS
CALU-6
U251
TUMORS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Brucella abortus (Ba) is an intracellular pathogen capable of surviving inside macrophages. Since the disease is presented in multiple forms, many different cells are susceptible to be infected by Ba. We have previously demonstrated that Ba RNA diminishes the IFN-ɤ-induced MHC-I surface expression in human macrophages, retaining them within the Golgi Apparatus (GA). However, we acknowledged whether this event could be triggered in other cells able to be infected with Ba. So, we stimulated the lung epithelium cell line (Calu-6) and the endothelial microvasculature cell line (HMEC) with different doses of Ba RNA in the presence of IFN-ɤ. MHC-I expression was assessed by flow cytometry. Ba RNA (10 ug/ml) diminished the IFN-ɤ-induced MHC-I surface expression (p<0.05) in both cell lines. Next, by confocal microscopy, we observed colocalization of MHC-I and GA marker GM130 in CALU-6 cells treated for 48h, although to a lesser extent than in human macrophages (p<0.05). Conversely to what we expected, supernatants from Calu-6-Ba RNA-treated cells had higher IL-8 production compared to those from untreated cells (p<0.05). In addition, a decrease in MHC-I on the surface could be accompanied by an activation of Natural Killer (NK) cells. These cells are key for the defense against multiple tumors. Therefore, a question of great importance is: what is the relevance of MHC-I modulation in the context of other pathologies in which NK response is critical? For this we started by stimulating the human glioblastoma cells (U251) with different doses of Ba RNA in the presence of IFN-ɤ. MHC-I expression was assessed by flow cytometry. Ba RNA diminished the IFN-ɤ-induced MHC-I surface expression (p<0.05) in U251. Together these results show that Ba could persist successfully within the host, remaining unnoticed and evading CD8+ T cell surveillance. On the other hand, the decrease in MHC-I could enhance the subsequent cytotoxic response against multiple tumors.
Fil: Serafino, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Bertinat, Yasmín Ayelén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Bueno, Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Milillo, María Ayelén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Barrionuevo, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Reunión Anual de Sociedades de Biociencias 2022; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad argentina de inmunología; Reunión anual 2022 de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
description Brucella abortus (Ba) is an intracellular pathogen capable of surviving inside macrophages. Since the disease is presented in multiple forms, many different cells are susceptible to be infected by Ba. We have previously demonstrated that Ba RNA diminishes the IFN-ɤ-induced MHC-I surface expression in human macrophages, retaining them within the Golgi Apparatus (GA). However, we acknowledged whether this event could be triggered in other cells able to be infected with Ba. So, we stimulated the lung epithelium cell line (Calu-6) and the endothelial microvasculature cell line (HMEC) with different doses of Ba RNA in the presence of IFN-ɤ. MHC-I expression was assessed by flow cytometry. Ba RNA (10 ug/ml) diminished the IFN-ɤ-induced MHC-I surface expression (p<0.05) in both cell lines. Next, by confocal microscopy, we observed colocalization of MHC-I and GA marker GM130 in CALU-6 cells treated for 48h, although to a lesser extent than in human macrophages (p<0.05). Conversely to what we expected, supernatants from Calu-6-Ba RNA-treated cells had higher IL-8 production compared to those from untreated cells (p<0.05). In addition, a decrease in MHC-I on the surface could be accompanied by an activation of Natural Killer (NK) cells. These cells are key for the defense against multiple tumors. Therefore, a question of great importance is: what is the relevance of MHC-I modulation in the context of other pathologies in which NK response is critical? For this we started by stimulating the human glioblastoma cells (U251) with different doses of Ba RNA in the presence of IFN-ɤ. MHC-I expression was assessed by flow cytometry. Ba RNA diminished the IFN-ɤ-induced MHC-I surface expression (p<0.05) in U251. Together these results show that Ba could persist successfully within the host, remaining unnoticed and evading CD8+ T cell surveillance. On the other hand, the decrease in MHC-I could enhance the subsequent cytotoxic response against multiple tumors.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/237490
Brucella abortus RNA diminishes IFN-gamma-induced MHC-I molecules in different line regular and tumor cells; Reunión Anual de Sociedades de Biociencias 2022; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad argentina de inmunología; Reunión anual 2022 de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2022; 150-150
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/237490
identifier_str_mv Brucella abortus RNA diminishes IFN-gamma-induced MHC-I molecules in different line regular and tumor cells; Reunión Anual de Sociedades de Biociencias 2022; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad argentina de inmunología; Reunión anual 2022 de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2022; 150-150
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2020/
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dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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