Beyond its preferential niche: Brucella abortus RNA down-modulates the IFN-γ-induced MHC-I expression in epithelial and endothelial cells
- Autores
- Serafino, Agustina; Bertinat, Yasmín Ayelén; Bueno, Jorgelina; Pittaluga, Jose; Birnberg Weiss, Federico; Milillo, María Ayelén; Barrionuevo, Paula
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Brucella abortus (Ba) is a pathogen that survives inside macrophages. Despite being itspreferential niche, Ba infects other cells, as shown by the multiple signs and symptomshumans present. This pathogen can evade our immune system. Ba displays a mechanismof down-modulating MHC-I on monocytes/macrophages in the presence of IFN-γ (whenTh1 response is triggered) without altering the total expression of MHC-I. The retainedMHC-I proteins are located within the Golgi Apparatus (GA). The RNA of Ba is one of thePAMPs that trigger this phenomenon. However, we acknowledged whether this event couldbe triggered in other cells relevant during Ba infection. Here, we demonstrate that Ba RNAreduced the surface expression of MHC-I induced by IFN-γ in the human bronchial epithelium(Calu-6), the human alveolar epithelium (A-549) and the endothelial microvasculature(HMEC) cell lines. In Calu-6 and HMEC cells, Ba RNA induces the retention of MHC-I in theGA. This phenomenon was not observed in A-549 cells. We then evaluated the effect of BaRNA on the secretion of IL-8, IL-6 and MCP-1, key cytokines in Ba infection. Contrary to ourexpectations, HMEC, Calu-6 and A-549 cells treated with Ba RNA had higher IL-8 and IL-6levels compared to untreated cells. In addition, we showed that Ba RNA down-modulatesthe MHC-I surface expression induced by IFN-γ on human monocytes/macrophages via thepathway of the Epidermal Growth Factor Receptor (EGFR). So, cells were stimulated withan EGFR ligand-blocking antibody (Cetuximab) and Ba RNA. Neutralization of the EGFR tosome extent reversed the down-modulation of MHC-I mediated by Ba RNA in HMEC and A-549 cells. In conclusion, this is the first study exploring a central immune evasion strategy,such as the downregulation of MHC-I surface expression, beyond monocytes and couldshed light on how it persists effectively within the host, enduring unseen and escaping CD8+T cell surveillance.
Fil: Serafino, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Bertinat, Yasmín Ayelén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Bueno, Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Pittaluga, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Birnberg Weiss, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Milillo, María Ayelén. Universidad Nacional de Río Negro. Sede Andina. Centro de Estudios en Ciencia, Tecnología, Cultura y Desarrollo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barrionuevo, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina - Materia
-
BRUCELLA ABORTUS
RNA
ENDOTHELIAL CELLS
EPITHELIAL CELLS
IMMUNE EVASION STRATEGIES - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/240939
Ver los metadatos del registro completo
id |
CONICETDig_73f54abcf5447c051cf3386e1b076d97 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/240939 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Beyond its preferential niche: Brucella abortus RNA down-modulates the IFN-γ-induced MHC-I expression in epithelial and endothelial cellsSerafino, AgustinaBertinat, Yasmín AyelénBueno, JorgelinaPittaluga, JoseBirnberg Weiss, FedericoMilillo, María AyelénBarrionuevo, PaulaBRUCELLA ABORTUSRNAENDOTHELIAL CELLSEPITHELIAL CELLSIMMUNE EVASION STRATEGIEShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Brucella abortus (Ba) is a pathogen that survives inside macrophages. Despite being itspreferential niche, Ba infects other cells, as shown by the multiple signs and symptomshumans present. This pathogen can evade our immune system. Ba displays a mechanismof down-modulating MHC-I on monocytes/macrophages in the presence of IFN-γ (whenTh1 response is triggered) without altering the total expression of MHC-I. The retainedMHC-I proteins are located within the Golgi Apparatus (GA). The RNA of Ba is one of thePAMPs that trigger this phenomenon. However, we acknowledged whether this event couldbe triggered in other cells relevant during Ba infection. Here, we demonstrate that Ba RNAreduced the surface expression of MHC-I induced by IFN-γ in the human bronchial epithelium(Calu-6), the human alveolar epithelium (A-549) and the endothelial microvasculature(HMEC) cell lines. In Calu-6 and HMEC cells, Ba RNA induces the retention of MHC-I in theGA. This phenomenon was not observed in A-549 cells. We then evaluated the effect of BaRNA on the secretion of IL-8, IL-6 and MCP-1, key cytokines in Ba infection. Contrary to ourexpectations, HMEC, Calu-6 and A-549 cells treated with Ba RNA had higher IL-8 and IL-6levels compared to untreated cells. In addition, we showed that Ba RNA down-modulatesthe MHC-I surface expression induced by IFN-γ on human monocytes/macrophages via thepathway of the Epidermal Growth Factor Receptor (EGFR). So, cells were stimulated withan EGFR ligand-blocking antibody (Cetuximab) and Ba RNA. Neutralization of the EGFR tosome extent reversed the down-modulation of MHC-I mediated by Ba RNA in HMEC and A-549 cells. In conclusion, this is the first study exploring a central immune evasion strategy,such as the downregulation of MHC-I surface expression, beyond monocytes and couldshed light on how it persists effectively within the host, enduring unseen and escaping CD8+T cell surveillance.Fil: Serafino, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Bertinat, Yasmín Ayelén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Bueno, Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Pittaluga, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Birnberg Weiss, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Milillo, María Ayelén. Universidad Nacional de Río Negro. Sede Andina. Centro de Estudios en Ciencia, Tecnología, Cultura y Desarrollo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barrionuevo, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaPublic Library of Science2024-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/240939Serafino, Agustina; Bertinat, Yasmín Ayelén; Bueno, Jorgelina; Pittaluga, Jose; Birnberg Weiss, Federico; et al.; Beyond its preferential niche: Brucella abortus RNA down-modulates the IFN-γ-induced MHC-I expression in epithelial and endothelial cells; Public Library of Science; Plos One; 19; 7; 7-2024; 1-161932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://dx.plos.org/10.1371/journal.pone.0306429info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0306429info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:59Zoai:ri.conicet.gov.ar:11336/240939instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:59.434CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Beyond its preferential niche: Brucella abortus RNA down-modulates the IFN-γ-induced MHC-I expression in epithelial and endothelial cells |
title |
Beyond its preferential niche: Brucella abortus RNA down-modulates the IFN-γ-induced MHC-I expression in epithelial and endothelial cells |
spellingShingle |
Beyond its preferential niche: Brucella abortus RNA down-modulates the IFN-γ-induced MHC-I expression in epithelial and endothelial cells Serafino, Agustina BRUCELLA ABORTUS RNA ENDOTHELIAL CELLS EPITHELIAL CELLS IMMUNE EVASION STRATEGIES |
title_short |
Beyond its preferential niche: Brucella abortus RNA down-modulates the IFN-γ-induced MHC-I expression in epithelial and endothelial cells |
title_full |
Beyond its preferential niche: Brucella abortus RNA down-modulates the IFN-γ-induced MHC-I expression in epithelial and endothelial cells |
title_fullStr |
Beyond its preferential niche: Brucella abortus RNA down-modulates the IFN-γ-induced MHC-I expression in epithelial and endothelial cells |
title_full_unstemmed |
Beyond its preferential niche: Brucella abortus RNA down-modulates the IFN-γ-induced MHC-I expression in epithelial and endothelial cells |
title_sort |
Beyond its preferential niche: Brucella abortus RNA down-modulates the IFN-γ-induced MHC-I expression in epithelial and endothelial cells |
dc.creator.none.fl_str_mv |
Serafino, Agustina Bertinat, Yasmín Ayelén Bueno, Jorgelina Pittaluga, Jose Birnberg Weiss, Federico Milillo, María Ayelén Barrionuevo, Paula |
author |
Serafino, Agustina |
author_facet |
Serafino, Agustina Bertinat, Yasmín Ayelén Bueno, Jorgelina Pittaluga, Jose Birnberg Weiss, Federico Milillo, María Ayelén Barrionuevo, Paula |
author_role |
author |
author2 |
Bertinat, Yasmín Ayelén Bueno, Jorgelina Pittaluga, Jose Birnberg Weiss, Federico Milillo, María Ayelén Barrionuevo, Paula |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
BRUCELLA ABORTUS RNA ENDOTHELIAL CELLS EPITHELIAL CELLS IMMUNE EVASION STRATEGIES |
topic |
BRUCELLA ABORTUS RNA ENDOTHELIAL CELLS EPITHELIAL CELLS IMMUNE EVASION STRATEGIES |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Brucella abortus (Ba) is a pathogen that survives inside macrophages. Despite being itspreferential niche, Ba infects other cells, as shown by the multiple signs and symptomshumans present. This pathogen can evade our immune system. Ba displays a mechanismof down-modulating MHC-I on monocytes/macrophages in the presence of IFN-γ (whenTh1 response is triggered) without altering the total expression of MHC-I. The retainedMHC-I proteins are located within the Golgi Apparatus (GA). The RNA of Ba is one of thePAMPs that trigger this phenomenon. However, we acknowledged whether this event couldbe triggered in other cells relevant during Ba infection. Here, we demonstrate that Ba RNAreduced the surface expression of MHC-I induced by IFN-γ in the human bronchial epithelium(Calu-6), the human alveolar epithelium (A-549) and the endothelial microvasculature(HMEC) cell lines. In Calu-6 and HMEC cells, Ba RNA induces the retention of MHC-I in theGA. This phenomenon was not observed in A-549 cells. We then evaluated the effect of BaRNA on the secretion of IL-8, IL-6 and MCP-1, key cytokines in Ba infection. Contrary to ourexpectations, HMEC, Calu-6 and A-549 cells treated with Ba RNA had higher IL-8 and IL-6levels compared to untreated cells. In addition, we showed that Ba RNA down-modulatesthe MHC-I surface expression induced by IFN-γ on human monocytes/macrophages via thepathway of the Epidermal Growth Factor Receptor (EGFR). So, cells were stimulated withan EGFR ligand-blocking antibody (Cetuximab) and Ba RNA. Neutralization of the EGFR tosome extent reversed the down-modulation of MHC-I mediated by Ba RNA in HMEC and A-549 cells. In conclusion, this is the first study exploring a central immune evasion strategy,such as the downregulation of MHC-I surface expression, beyond monocytes and couldshed light on how it persists effectively within the host, enduring unseen and escaping CD8+T cell surveillance. Fil: Serafino, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Bertinat, Yasmín Ayelén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Bueno, Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Pittaluga, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Birnberg Weiss, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Milillo, María Ayelén. Universidad Nacional de Río Negro. Sede Andina. Centro de Estudios en Ciencia, Tecnología, Cultura y Desarrollo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Barrionuevo, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina |
description |
Brucella abortus (Ba) is a pathogen that survives inside macrophages. Despite being itspreferential niche, Ba infects other cells, as shown by the multiple signs and symptomshumans present. This pathogen can evade our immune system. Ba displays a mechanismof down-modulating MHC-I on monocytes/macrophages in the presence of IFN-γ (whenTh1 response is triggered) without altering the total expression of MHC-I. The retainedMHC-I proteins are located within the Golgi Apparatus (GA). The RNA of Ba is one of thePAMPs that trigger this phenomenon. However, we acknowledged whether this event couldbe triggered in other cells relevant during Ba infection. Here, we demonstrate that Ba RNAreduced the surface expression of MHC-I induced by IFN-γ in the human bronchial epithelium(Calu-6), the human alveolar epithelium (A-549) and the endothelial microvasculature(HMEC) cell lines. In Calu-6 and HMEC cells, Ba RNA induces the retention of MHC-I in theGA. This phenomenon was not observed in A-549 cells. We then evaluated the effect of BaRNA on the secretion of IL-8, IL-6 and MCP-1, key cytokines in Ba infection. Contrary to ourexpectations, HMEC, Calu-6 and A-549 cells treated with Ba RNA had higher IL-8 and IL-6levels compared to untreated cells. In addition, we showed that Ba RNA down-modulatesthe MHC-I surface expression induced by IFN-γ on human monocytes/macrophages via thepathway of the Epidermal Growth Factor Receptor (EGFR). So, cells were stimulated withan EGFR ligand-blocking antibody (Cetuximab) and Ba RNA. Neutralization of the EGFR tosome extent reversed the down-modulation of MHC-I mediated by Ba RNA in HMEC and A-549 cells. In conclusion, this is the first study exploring a central immune evasion strategy,such as the downregulation of MHC-I surface expression, beyond monocytes and couldshed light on how it persists effectively within the host, enduring unseen and escaping CD8+T cell surveillance. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/240939 Serafino, Agustina; Bertinat, Yasmín Ayelén; Bueno, Jorgelina; Pittaluga, Jose; Birnberg Weiss, Federico; et al.; Beyond its preferential niche: Brucella abortus RNA down-modulates the IFN-γ-induced MHC-I expression in epithelial and endothelial cells; Public Library of Science; Plos One; 19; 7; 7-2024; 1-16 1932-6203 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/240939 |
identifier_str_mv |
Serafino, Agustina; Bertinat, Yasmín Ayelén; Bueno, Jorgelina; Pittaluga, Jose; Birnberg Weiss, Federico; et al.; Beyond its preferential niche: Brucella abortus RNA down-modulates the IFN-γ-induced MHC-I expression in epithelial and endothelial cells; Public Library of Science; Plos One; 19; 7; 7-2024; 1-16 1932-6203 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://dx.plos.org/10.1371/journal.pone.0306429 info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0306429 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613568858685440 |
score |
13.070432 |