Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxia
- Autores
- Lombardo, Tomás; Peralta, Diego Matias; Kornblihtt, L.; Blanco, Guillermo Armando C.
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Themiste petricola is a marine intertidal endolithic worm that experiences transient hypoxia within its habitat, owing to natural sediment movements or increased organic enrichment. We characterized and quantified the cytotoxic effect of H2O2 in celomocytes of the sipunculan Themiste petricola under normoxia and hypoxia (O2 < 0.1 %) through the median effect method. The 50 % cell death H2O2 dose at 24 h (EC50) under normoxia was 1.5 mM. The range EC10-EC90 was 0.6 mM - 3.9 mM. The fraction of cells having collapsed mitochondrial membrane potential (MMP) was increased dose-dependently after 3 h exposure with 24 h cytotoxic doses of H2O2 from EC10 to EC90. The 24 h cytotoxic dose inducing 50 % of cells with collapsed MMP at 3 h was 3.67 mM. Intracellular superoxide anion production was increased dose-dependently, while reduced glutathione was decreased dose-dependently at 3 h with H2O2 from EC10 to EC90. Exposure to 24 h hypoxia did not cause cell death but induced intracellular acidification. The 24 h EC50 of H2O2 under hypoxia was increased to 4.7 mM while the range EC10-EC90 was increased to 0.9 mM - 25.1 mM. We conclude that hypoxia induces anaerobic metabolism and increases tolerance to H2O2-induced cell death in celomocytes of Themiste petricola preserving the immune functions and providing an advantage to survive under low oxygen tension.
Fil: Lombardo, Tomás. Consejo Nacional de Investigaciones Cientiâficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Fil: Peralta, Diego Matias. Consejo Nacional de Investigaciones Cientiâficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Fil: Kornblihtt, L.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Blanco, Guillermo Armando C.. Consejo Nacional de Investigaciones Cientiâficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina - Materia
-
Sipuncula
Hypoxia
Hydrogen peroxide
ROS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/8042
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Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxiaLombardo, TomásPeralta, Diego MatiasKornblihtt, L.Blanco, Guillermo Armando C.SipunculaHypoxiaHydrogen peroxideROShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Themiste petricola is a marine intertidal endolithic worm that experiences transient hypoxia within its habitat, owing to natural sediment movements or increased organic enrichment. We characterized and quantified the cytotoxic effect of H2O2 in celomocytes of the sipunculan Themiste petricola under normoxia and hypoxia (O2 < 0.1 %) through the median effect method. The 50 % cell death H2O2 dose at 24 h (EC50) under normoxia was 1.5 mM. The range EC10-EC90 was 0.6 mM - 3.9 mM. The fraction of cells having collapsed mitochondrial membrane potential (MMP) was increased dose-dependently after 3 h exposure with 24 h cytotoxic doses of H2O2 from EC10 to EC90. The 24 h cytotoxic dose inducing 50 % of cells with collapsed MMP at 3 h was 3.67 mM. Intracellular superoxide anion production was increased dose-dependently, while reduced glutathione was decreased dose-dependently at 3 h with H2O2 from EC10 to EC90. Exposure to 24 h hypoxia did not cause cell death but induced intracellular acidification. The 24 h EC50 of H2O2 under hypoxia was increased to 4.7 mM while the range EC10-EC90 was increased to 0.9 mM - 25.1 mM. We conclude that hypoxia induces anaerobic metabolism and increases tolerance to H2O2-induced cell death in celomocytes of Themiste petricola preserving the immune functions and providing an advantage to survive under low oxygen tension.Fil: Lombardo, Tomás. Consejo Nacional de Investigaciones Cientiâficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; ArgentinaFil: Peralta, Diego Matias. Consejo Nacional de Investigaciones Cientiâficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; ArgentinaFil: Kornblihtt, L.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Blanco, Guillermo Armando C.. Consejo Nacional de Investigaciones Cientiâficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; ArgentinaUniversidad de Modena2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8042Lombardo, Tomás; Peralta, Diego Matias; Kornblihtt, L.; Blanco, Guillermo Armando C.; Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxia; Universidad de Modena; Invertebrate Survival Journal; 11; 4-2014; 87-1021824-307Xenginfo:eu-repo/semantics/altIdentifier/url/http://www.isj.unimo.it/articoli/ISJ318.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:10:01Zoai:ri.conicet.gov.ar:11336/8042instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:10:01.41CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxia |
title |
Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxia |
spellingShingle |
Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxia Lombardo, Tomás Sipuncula Hypoxia Hydrogen peroxide ROS |
title_short |
Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxia |
title_full |
Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxia |
title_fullStr |
Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxia |
title_full_unstemmed |
Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxia |
title_sort |
Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxia |
dc.creator.none.fl_str_mv |
Lombardo, Tomás Peralta, Diego Matias Kornblihtt, L. Blanco, Guillermo Armando C. |
author |
Lombardo, Tomás |
author_facet |
Lombardo, Tomás Peralta, Diego Matias Kornblihtt, L. Blanco, Guillermo Armando C. |
author_role |
author |
author2 |
Peralta, Diego Matias Kornblihtt, L. Blanco, Guillermo Armando C. |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Sipuncula Hypoxia Hydrogen peroxide ROS |
topic |
Sipuncula Hypoxia Hydrogen peroxide ROS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Themiste petricola is a marine intertidal endolithic worm that experiences transient hypoxia within its habitat, owing to natural sediment movements or increased organic enrichment. We characterized and quantified the cytotoxic effect of H2O2 in celomocytes of the sipunculan Themiste petricola under normoxia and hypoxia (O2 < 0.1 %) through the median effect method. The 50 % cell death H2O2 dose at 24 h (EC50) under normoxia was 1.5 mM. The range EC10-EC90 was 0.6 mM - 3.9 mM. The fraction of cells having collapsed mitochondrial membrane potential (MMP) was increased dose-dependently after 3 h exposure with 24 h cytotoxic doses of H2O2 from EC10 to EC90. The 24 h cytotoxic dose inducing 50 % of cells with collapsed MMP at 3 h was 3.67 mM. Intracellular superoxide anion production was increased dose-dependently, while reduced glutathione was decreased dose-dependently at 3 h with H2O2 from EC10 to EC90. Exposure to 24 h hypoxia did not cause cell death but induced intracellular acidification. The 24 h EC50 of H2O2 under hypoxia was increased to 4.7 mM while the range EC10-EC90 was increased to 0.9 mM - 25.1 mM. We conclude that hypoxia induces anaerobic metabolism and increases tolerance to H2O2-induced cell death in celomocytes of Themiste petricola preserving the immune functions and providing an advantage to survive under low oxygen tension. Fil: Lombardo, Tomás. Consejo Nacional de Investigaciones Cientiâficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina Fil: Peralta, Diego Matias. Consejo Nacional de Investigaciones Cientiâficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina Fil: Kornblihtt, L.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina Fil: Blanco, Guillermo Armando C.. Consejo Nacional de Investigaciones Cientiâficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina |
description |
Themiste petricola is a marine intertidal endolithic worm that experiences transient hypoxia within its habitat, owing to natural sediment movements or increased organic enrichment. We characterized and quantified the cytotoxic effect of H2O2 in celomocytes of the sipunculan Themiste petricola under normoxia and hypoxia (O2 < 0.1 %) through the median effect method. The 50 % cell death H2O2 dose at 24 h (EC50) under normoxia was 1.5 mM. The range EC10-EC90 was 0.6 mM - 3.9 mM. The fraction of cells having collapsed mitochondrial membrane potential (MMP) was increased dose-dependently after 3 h exposure with 24 h cytotoxic doses of H2O2 from EC10 to EC90. The 24 h cytotoxic dose inducing 50 % of cells with collapsed MMP at 3 h was 3.67 mM. Intracellular superoxide anion production was increased dose-dependently, while reduced glutathione was decreased dose-dependently at 3 h with H2O2 from EC10 to EC90. Exposure to 24 h hypoxia did not cause cell death but induced intracellular acidification. The 24 h EC50 of H2O2 under hypoxia was increased to 4.7 mM while the range EC10-EC90 was increased to 0.9 mM - 25.1 mM. We conclude that hypoxia induces anaerobic metabolism and increases tolerance to H2O2-induced cell death in celomocytes of Themiste petricola preserving the immune functions and providing an advantage to survive under low oxygen tension. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/8042 Lombardo, Tomás; Peralta, Diego Matias; Kornblihtt, L.; Blanco, Guillermo Armando C.; Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxia; Universidad de Modena; Invertebrate Survival Journal; 11; 4-2014; 87-102 1824-307X |
url |
http://hdl.handle.net/11336/8042 |
identifier_str_mv |
Lombardo, Tomás; Peralta, Diego Matias; Kornblihtt, L.; Blanco, Guillermo Armando C.; Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxia; Universidad de Modena; Invertebrate Survival Journal; 11; 4-2014; 87-102 1824-307X |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.isj.unimo.it/articoli/ISJ318.pdf |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidad de Modena |
publisher.none.fl_str_mv |
Universidad de Modena |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613985280720896 |
score |
13.070432 |