Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxia

Autores
Lombardo, Tomás; Peralta, Diego Matias; Kornblihtt, L.; Blanco, Guillermo Armando C.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Themiste petricola is a marine intertidal endolithic worm that experiences transient hypoxia within its habitat, owing to natural sediment movements or increased organic enrichment. We characterized and quantified the cytotoxic effect of H2O2 in celomocytes of the sipunculan Themiste petricola under normoxia and hypoxia (O2 < 0.1 %) through the median effect method. The 50 % cell death H2O2 dose at 24 h (EC50) under normoxia was 1.5 mM. The range EC10-EC90 was 0.6 mM - 3.9 mM. The fraction of cells having collapsed mitochondrial membrane potential (MMP) was increased dose-dependently after 3 h exposure with 24 h cytotoxic doses of H2O2 from EC10 to EC90. The 24 h cytotoxic dose inducing 50 % of cells with collapsed MMP at 3 h was 3.67 mM. Intracellular superoxide anion production was increased dose-dependently, while reduced glutathione was decreased dose-dependently at 3 h with H2O2 from EC10 to EC90. Exposure to 24 h hypoxia did not cause cell death but induced intracellular acidification. The 24 h EC50 of H2O2 under hypoxia was increased to 4.7 mM while the range EC10-EC90 was increased to 0.9 mM - 25.1 mM. We conclude that hypoxia induces anaerobic metabolism and increases tolerance to H2O2-induced cell death in celomocytes of Themiste petricola preserving the immune functions and providing an advantage to survive under low oxygen tension.
Fil: Lombardo, Tomás. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Fil: Peralta, Diego Matias. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Fil: Kornblihtt, L.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Blanco, Guillermo Armando C.. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Materia
Sipuncula
Hypoxia
Hydrogen peroxide
ROS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8042

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network_name_str CONICET Digital (CONICET)
spelling Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxiaLombardo, TomásPeralta, Diego MatiasKornblihtt, L.Blanco, Guillermo Armando C.SipunculaHypoxiaHydrogen peroxideROShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Themiste petricola is a marine intertidal endolithic worm that experiences transient hypoxia within its habitat, owing to natural sediment movements or increased organic enrichment. We characterized and quantified the cytotoxic effect of H2O2 in celomocytes of the sipunculan Themiste petricola under normoxia and hypoxia (O2 < 0.1 %) through the median effect method. The 50 % cell death H2O2 dose at 24 h (EC50) under normoxia was 1.5 mM. The range EC10-EC90 was 0.6 mM - 3.9 mM. The fraction of cells having collapsed mitochondrial membrane potential (MMP) was increased dose-dependently after 3 h exposure with 24 h cytotoxic doses of H2O2 from EC10 to EC90. The 24 h cytotoxic dose inducing 50 % of cells with collapsed MMP at 3 h was 3.67 mM. Intracellular superoxide anion production was increased dose-dependently, while reduced glutathione was decreased dose-dependently at 3 h with H2O2 from EC10 to EC90. Exposure to 24 h hypoxia did not cause cell death but induced intracellular acidification. The 24 h EC50 of H2O2 under hypoxia was increased to 4.7 mM while the range EC10-EC90 was increased to 0.9 mM - 25.1 mM. We conclude that hypoxia induces anaerobic metabolism and increases tolerance to H2O2-induced cell death in celomocytes of Themiste petricola preserving the immune functions and providing an advantage to survive under low oxygen tension.Fil: Lombardo, Tomás. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; ArgentinaFil: Peralta, Diego Matias. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; ArgentinaFil: Kornblihtt, L.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Blanco, Guillermo Armando C.. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; ArgentinaUniversidad de Modena2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8042Lombardo, Tomás; Peralta, Diego Matias; Kornblihtt, L.; Blanco, Guillermo Armando C.; Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxia; Universidad de Modena; Invertebrate Survival Journal; 11; 4-2014; 87-1021824-307Xenginfo:eu-repo/semantics/altIdentifier/url/http://www.isj.unimo.it/articoli/ISJ318.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:10:01Zoai:ri.conicet.gov.ar:11336/8042instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:10:01.41CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxia
title Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxia
spellingShingle Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxia
Lombardo, Tomás
Sipuncula
Hypoxia
Hydrogen peroxide
ROS
title_short Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxia
title_full Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxia
title_fullStr Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxia
title_full_unstemmed Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxia
title_sort Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxia
dc.creator.none.fl_str_mv Lombardo, Tomás
Peralta, Diego Matias
Kornblihtt, L.
Blanco, Guillermo Armando C.
author Lombardo, Tomás
author_facet Lombardo, Tomás
Peralta, Diego Matias
Kornblihtt, L.
Blanco, Guillermo Armando C.
author_role author
author2 Peralta, Diego Matias
Kornblihtt, L.
Blanco, Guillermo Armando C.
author2_role author
author
author
dc.subject.none.fl_str_mv Sipuncula
Hypoxia
Hydrogen peroxide
ROS
topic Sipuncula
Hypoxia
Hydrogen peroxide
ROS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Themiste petricola is a marine intertidal endolithic worm that experiences transient hypoxia within its habitat, owing to natural sediment movements or increased organic enrichment. We characterized and quantified the cytotoxic effect of H2O2 in celomocytes of the sipunculan Themiste petricola under normoxia and hypoxia (O2 < 0.1 %) through the median effect method. The 50 % cell death H2O2 dose at 24 h (EC50) under normoxia was 1.5 mM. The range EC10-EC90 was 0.6 mM - 3.9 mM. The fraction of cells having collapsed mitochondrial membrane potential (MMP) was increased dose-dependently after 3 h exposure with 24 h cytotoxic doses of H2O2 from EC10 to EC90. The 24 h cytotoxic dose inducing 50 % of cells with collapsed MMP at 3 h was 3.67 mM. Intracellular superoxide anion production was increased dose-dependently, while reduced glutathione was decreased dose-dependently at 3 h with H2O2 from EC10 to EC90. Exposure to 24 h hypoxia did not cause cell death but induced intracellular acidification. The 24 h EC50 of H2O2 under hypoxia was increased to 4.7 mM while the range EC10-EC90 was increased to 0.9 mM - 25.1 mM. We conclude that hypoxia induces anaerobic metabolism and increases tolerance to H2O2-induced cell death in celomocytes of Themiste petricola preserving the immune functions and providing an advantage to survive under low oxygen tension.
Fil: Lombardo, Tomás. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Fil: Peralta, Diego Matias. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Fil: Kornblihtt, L.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Blanco, Guillermo Armando C.. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
description Themiste petricola is a marine intertidal endolithic worm that experiences transient hypoxia within its habitat, owing to natural sediment movements or increased organic enrichment. We characterized and quantified the cytotoxic effect of H2O2 in celomocytes of the sipunculan Themiste petricola under normoxia and hypoxia (O2 < 0.1 %) through the median effect method. The 50 % cell death H2O2 dose at 24 h (EC50) under normoxia was 1.5 mM. The range EC10-EC90 was 0.6 mM - 3.9 mM. The fraction of cells having collapsed mitochondrial membrane potential (MMP) was increased dose-dependently after 3 h exposure with 24 h cytotoxic doses of H2O2 from EC10 to EC90. The 24 h cytotoxic dose inducing 50 % of cells with collapsed MMP at 3 h was 3.67 mM. Intracellular superoxide anion production was increased dose-dependently, while reduced glutathione was decreased dose-dependently at 3 h with H2O2 from EC10 to EC90. Exposure to 24 h hypoxia did not cause cell death but induced intracellular acidification. The 24 h EC50 of H2O2 under hypoxia was increased to 4.7 mM while the range EC10-EC90 was increased to 0.9 mM - 25.1 mM. We conclude that hypoxia induces anaerobic metabolism and increases tolerance to H2O2-induced cell death in celomocytes of Themiste petricola preserving the immune functions and providing an advantage to survive under low oxygen tension.
publishDate 2014
dc.date.none.fl_str_mv 2014-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8042
Lombardo, Tomás; Peralta, Diego Matias; Kornblihtt, L.; Blanco, Guillermo Armando C.; Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxia; Universidad de Modena; Invertebrate Survival Journal; 11; 4-2014; 87-102
1824-307X
url http://hdl.handle.net/11336/8042
identifier_str_mv Lombardo, Tomás; Peralta, Diego Matias; Kornblihtt, L.; Blanco, Guillermo Armando C.; Sipunculan celomocytes increase the resistance to H2O2-induced cell death under hypoxia; Universidad de Modena; Invertebrate Survival Journal; 11; 4-2014; 87-102
1824-307X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.isj.unimo.it/articoli/ISJ318.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidad de Modena
publisher.none.fl_str_mv Universidad de Modena
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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