Presence of Fusarium graminearum sensu stricto associated with triticale (x. Triticosecale Wittmack) in Argentina

Autores
Dinolfo, María Inés; Martínez, Mauro; Castañares, Eliana; Stenglein, Sebastian Alberto
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Triticale is a promising crop resulting for crosses between wheat and rye. This crop has been used as animal feed but also for human food in the preparation of baking products. As regards to triticale diseases, few reports have evaluated the severity of pathogens on this crop. Fusarium Head Blight (FHB) affects several crops including triticale parent crops, with Fusarium graminearum sensu stricto (s.s.) being the most commonly isolated pathogen from plants showing FHB symptoms. The objective of this study was to report the occurrence of F. graminearum s.s. on triticale grains from field samples in Argentina. For this purpose, a total of 40 samples from different crops (barley, rye, triticale, and wheat) showing FHB symptoms were taken during 2017/2018 harvest season. Two Fusarium isolates were identified morphologically as F.graminearum , which were confirmed molecularly by PCR using the specific Fg16 F/R primers pair and by sequencing redand tri101 genes. The sequences obtained were compared with those available in the NCBI database by using BLAST tools showing 99-100% homology with those belonging to F. graminearum s.s.. The potential to produce DON, 15-ADON, and ZEA was determined. Also, the Koch´s postulates were carried out to evaluate the pathogenic capacity of the F. graminearum s.s. isolates in triticale. The results demonstrated that F. graminearum s.s. isolates were pathogenic when triticale spikes were inoculated by spraying under greenhouse conditions. To our knowledge, this is the first time that the presence of F. graminearum s.s. associated with 18 triticale from fields in Argentina was reported.
Fil: Dinolfo, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina
Fil: Martínez, Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina
Fil: Castañares, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina
Fil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina
Materia
FUSARIUM
TRITICALE
MYCOTOXINS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/107079
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Presence of Fusarium graminearum sensu stricto associated with triticale (x. Triticosecale Wittmack) in ArgentinaDinolfo, María InésMartínez, MauroCastañares, ElianaStenglein, Sebastian AlbertoFUSARIUMTRITICALEMYCOTOXINShttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Triticale is a promising crop resulting for crosses between wheat and rye. This crop has been used as animal feed but also for human food in the preparation of baking products. As regards to triticale diseases, few reports have evaluated the severity of pathogens on this crop. Fusarium Head Blight (FHB) affects several crops including triticale parent crops, with Fusarium graminearum sensu stricto (s.s.) being the most commonly isolated pathogen from plants showing FHB symptoms. The objective of this study was to report the occurrence of F. graminearum s.s. on triticale grains from field samples in Argentina. For this purpose, a total of 40 samples from different crops (barley, rye, triticale, and wheat) showing FHB symptoms were taken during 2017/2018 harvest season. Two Fusarium isolates were identified morphologically as F.graminearum , which were confirmed molecularly by PCR using the specific Fg16 F/R primers pair and by sequencing redand tri101 genes. The sequences obtained were compared with those available in the NCBI database by using BLAST tools showing 99-100% homology with those belonging to F. graminearum s.s.. The potential to produce DON, 15-ADON, and ZEA was determined. Also, the Koch´s postulates were carried out to evaluate the pathogenic capacity of the F. graminearum s.s. isolates in triticale. The results demonstrated that F. graminearum s.s. isolates were pathogenic when triticale spikes were inoculated by spraying under greenhouse conditions. To our knowledge, this is the first time that the presence of F. graminearum s.s. associated with 18 triticale from fields in Argentina was reported.Fil: Dinolfo, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; ArgentinaFil: Martínez, Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; ArgentinaFil: Castañares, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; ArgentinaFil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; ArgentinaSpanish National Institute for Agriculture and Food Research and Technology2020-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/107079Dinolfo, María Inés; Martínez, Mauro; Castañares, Eliana; Stenglein, Sebastian Alberto; Presence of Fusarium graminearum sensu stricto associated with triticale (x. Triticosecale Wittmack) in Argentina; Spanish National Institute for Agriculture and Food Research and Technology; Spanish Journal Of Agricultural Research; 18; 1-20201695-971XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.5424/sjar/2020181-14796info:eu-repo/semantics/altIdentifier/url/https://revistas.inia.es/index.php/sjar/article/view/14796info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:37:32Zoai:ri.conicet.gov.ar:11336/107079instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:37:32.799CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Presence of Fusarium graminearum sensu stricto associated with triticale (x. Triticosecale Wittmack) in Argentina
title Presence of Fusarium graminearum sensu stricto associated with triticale (x. Triticosecale Wittmack) in Argentina
spellingShingle Presence of Fusarium graminearum sensu stricto associated with triticale (x. Triticosecale Wittmack) in Argentina
Dinolfo, María Inés
FUSARIUM
TRITICALE
MYCOTOXINS
title_short Presence of Fusarium graminearum sensu stricto associated with triticale (x. Triticosecale Wittmack) in Argentina
title_full Presence of Fusarium graminearum sensu stricto associated with triticale (x. Triticosecale Wittmack) in Argentina
title_fullStr Presence of Fusarium graminearum sensu stricto associated with triticale (x. Triticosecale Wittmack) in Argentina
title_full_unstemmed Presence of Fusarium graminearum sensu stricto associated with triticale (x. Triticosecale Wittmack) in Argentina
title_sort Presence of Fusarium graminearum sensu stricto associated with triticale (x. Triticosecale Wittmack) in Argentina
dc.creator.none.fl_str_mv Dinolfo, María Inés
Martínez, Mauro
Castañares, Eliana
Stenglein, Sebastian Alberto
author Dinolfo, María Inés
author_facet Dinolfo, María Inés
Martínez, Mauro
Castañares, Eliana
Stenglein, Sebastian Alberto
author_role author
author2 Martínez, Mauro
Castañares, Eliana
Stenglein, Sebastian Alberto
author2_role author
author
author
dc.subject.none.fl_str_mv FUSARIUM
TRITICALE
MYCOTOXINS
topic FUSARIUM
TRITICALE
MYCOTOXINS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Triticale is a promising crop resulting for crosses between wheat and rye. This crop has been used as animal feed but also for human food in the preparation of baking products. As regards to triticale diseases, few reports have evaluated the severity of pathogens on this crop. Fusarium Head Blight (FHB) affects several crops including triticale parent crops, with Fusarium graminearum sensu stricto (s.s.) being the most commonly isolated pathogen from plants showing FHB symptoms. The objective of this study was to report the occurrence of F. graminearum s.s. on triticale grains from field samples in Argentina. For this purpose, a total of 40 samples from different crops (barley, rye, triticale, and wheat) showing FHB symptoms were taken during 2017/2018 harvest season. Two Fusarium isolates were identified morphologically as F.graminearum , which were confirmed molecularly by PCR using the specific Fg16 F/R primers pair and by sequencing redand tri101 genes. The sequences obtained were compared with those available in the NCBI database by using BLAST tools showing 99-100% homology with those belonging to F. graminearum s.s.. The potential to produce DON, 15-ADON, and ZEA was determined. Also, the Koch´s postulates were carried out to evaluate the pathogenic capacity of the F. graminearum s.s. isolates in triticale. The results demonstrated that F. graminearum s.s. isolates were pathogenic when triticale spikes were inoculated by spraying under greenhouse conditions. To our knowledge, this is the first time that the presence of F. graminearum s.s. associated with 18 triticale from fields in Argentina was reported.
Fil: Dinolfo, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina
Fil: Martínez, Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina
Fil: Castañares, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina
Fil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina
description Triticale is a promising crop resulting for crosses between wheat and rye. This crop has been used as animal feed but also for human food in the preparation of baking products. As regards to triticale diseases, few reports have evaluated the severity of pathogens on this crop. Fusarium Head Blight (FHB) affects several crops including triticale parent crops, with Fusarium graminearum sensu stricto (s.s.) being the most commonly isolated pathogen from plants showing FHB symptoms. The objective of this study was to report the occurrence of F. graminearum s.s. on triticale grains from field samples in Argentina. For this purpose, a total of 40 samples from different crops (barley, rye, triticale, and wheat) showing FHB symptoms were taken during 2017/2018 harvest season. Two Fusarium isolates were identified morphologically as F.graminearum , which were confirmed molecularly by PCR using the specific Fg16 F/R primers pair and by sequencing redand tri101 genes. The sequences obtained were compared with those available in the NCBI database by using BLAST tools showing 99-100% homology with those belonging to F. graminearum s.s.. The potential to produce DON, 15-ADON, and ZEA was determined. Also, the Koch´s postulates were carried out to evaluate the pathogenic capacity of the F. graminearum s.s. isolates in triticale. The results demonstrated that F. graminearum s.s. isolates were pathogenic when triticale spikes were inoculated by spraying under greenhouse conditions. To our knowledge, this is the first time that the presence of F. graminearum s.s. associated with 18 triticale from fields in Argentina was reported.
publishDate 2020
dc.date.none.fl_str_mv 2020-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/107079
Dinolfo, María Inés; Martínez, Mauro; Castañares, Eliana; Stenglein, Sebastian Alberto; Presence of Fusarium graminearum sensu stricto associated with triticale (x. Triticosecale Wittmack) in Argentina; Spanish National Institute for Agriculture and Food Research and Technology; Spanish Journal Of Agricultural Research; 18; 1-2020
1695-971X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/107079
identifier_str_mv Dinolfo, María Inés; Martínez, Mauro; Castañares, Eliana; Stenglein, Sebastian Alberto; Presence of Fusarium graminearum sensu stricto associated with triticale (x. Triticosecale Wittmack) in Argentina; Spanish National Institute for Agriculture and Food Research and Technology; Spanish Journal Of Agricultural Research; 18; 1-2020
1695-971X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.5424/sjar/2020181-14796
info:eu-repo/semantics/altIdentifier/url/https://revistas.inia.es/index.php/sjar/article/view/14796
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Spanish National Institute for Agriculture and Food Research and Technology
publisher.none.fl_str_mv Spanish National Institute for Agriculture and Food Research and Technology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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